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biochemistry & molecular biology (32) 32
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African Journal of Biotechnology, ISSN 1684-5315, 12/2011, Volume 10, Issue 81, pp. 18829 - 18832
In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant... 
Ananas comosus | Bromelain | Ni-NTA his•bind resin | Fast protein liquid chromatography (FPLC) | Ni-NTA His center dot Bind resin | fast protein liquid chromatography (FPLC) | BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal Article
Turkish Journal of Veterinary and Animal Sciences, ISSN 1300-0128, 2017, Volume 41, Issue 5, pp. 656 - 666
Glutathione reductase (GR) is highly conserved among diverse taxa and has important biochemical functions. These functions may facilitate survival in harsh... 
PGEM-T Easy vector | Partial characterization | Recombinant glutathione reductase | Camelus dromedaries liver | Ni-NTA resin | partial characterization | recombinant glutathione reductase | RESPONSES | pGEM-T Easy vector | ERYTHROCYTES | METABOLISM | ENZYMES | PURIFICATION | VETERINARY SCIENCES | AFFINITY-CHROMATOGRAPHY | STRESS | EXPOSURE
Journal Article
BBA - Bioenergetics, ISSN 0005-2728, 2005, Volume 1706, Issue 1, pp. 110 - 116
His-tagged cysteine-less F F ATP synthase from was purified using Ni-NTA affinity chromatography. During the purification procedure the loss of total ATPase... 
ACMA | Reconstitution | Escherichia coli F 1F o ATP synthase | Protein purification | Steady-state kinetics | Ni-NTA resin | ATP synthase | F | Escherichia coli F
Journal Article
Journal Article
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, ISSN 0005-2728, 01/2005, Volume 1706, Issue 1-2, pp. 110 - 116
His-tagged cysteine-less F1F0 ATP synthase from Escherichia coli was purified using Ni-NTA affinity chromatography. During the purification procedure the loss... 
reconstitution | SUBMITOCHONDRIAL PARTICLES | F1-ATPASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | STOICHIOMETRY | protein purification | ACMA | HYDROLYSIS | MEMBRANES | ADENOSINE-TRIPHOSPHATASE | steady-state kinetics | EPSILON-SUBUNIT | ATP synthase | BIOPHYSICS | Ni-NTA resin | Escherichia coli F1F0 | H+-ATPASE | ROTARY MOTOR | LIPOSOMES
Journal Article
Journal Article
Journal of Biological Chemistry, ISSN 0021-9258, 02/2003, Volume 278, Issue 9, pp. 6719 - 6730
Journal Article
Journal Article
Journal of Biochemistry, ISSN 0021-924X, 1996, Volume 119, Issue 4, pp. 667 - 673
Journal Article
Journal Article
MEDICAL MICROBIOLOGY AND IMMUNOLOGY, ISSN 0300-8584, 11/1996, Volume 185, Issue 3, pp. 153 - 155
A 33-kDa protein (TB33) was isolated from a delipidated cell sonicate (CS) of Mycobacterium tuberculosis H(37)Rv (grown in Middlebrook 7H9 broth supplemented... 
ANTIBODIES | M-tuberculosis | K QU MICROBIOLOGY | PEPTIDES | delipidified | Ni-NTA resin | K NI IMMUNOLOGY | MICROBIOLOGY | IMMUNOLOGY | LEPRAE | immobilized metal affinity chromatography | EPITOPES
Journal Article
Medical Microbiology and Immunology, ISSN 0300-8584, 1996, Volume 185, Issue 3, pp. 153 - 155
A 33-kDa protein (TB33) was isolated from a delipidated cell sonicate (CS) of Mycobacterium tuberculosis H37Rv (grown in Middlebrook 7H9 broth supplemented... 
Immobilized metal affinity chromatography | Delipidified | M. tuberculosis | Ni-NTA resin | Animals | Chromatography, Affinity | Molecular Weight | Bacterial Proteins - chemistry | Lipids | Mycobacterium tuberculosis - chemistry | Mice | Bacterial Proteins - isolation & purification
Journal Article
生長激素是由脊椎動物的腦下垂腺所分泌.天然的生長激素生長不易,因此以基因工程 的方式來大量生產生長激素.從100 ml 的菌液中可以得到81.67 mg的內涵體,從其中可以 得到30.19 mg 折疊完全的生長激素.為簡化純化蛋白質的步驟,以Ni-NTA resin的方法來 純化帶有His-tag... 
生長激素 | 黃鰭鯛 | microencapsulate | 大腸桿菌 | Ni-NTA resin | growth hormone | 鎳離子親和性管柱 | yp porgy | E. coli | 微膠囊
Dissertation
Analytical Biochemistry, ISSN 0003-2697, 10/2019, Volume 582, p. 113347
The widespread use of immobilized metal-affinity chromatography (IMAC) for fast and efficient purification of recombinant proteins has brought potentially... 
Immobilized metal-affinity chromatography | Metal contamination | Ni-NTA | Hydroxynaphtol blue
Journal Article
Analytical Biochemistry, ISSN 0003-2697, 02/2020, Volume 590, p. 113518
Heat shock protein 90 (HSP90) plays essential roles in the normal physiology and comprises four distinct domains, including NH -terminal (N), charged linker... 
ATR-FTIR | Chaperone | Trx-his-S tag | Yield | Affinity purification | Mass spectrometry | Ni-NTA | Heat shock protein 90
Journal Article
Jundishapur Journal of Microbiology, ISSN 2008-3645, 08/2015, Volume 8, Issue 8, p. e23218
Background: Nontypeable Haemophilus influenzae (NTHi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and... 
Recombinant protein | Haemophilus influenzae | Ni-NTA | Expression vector | INFECTIONS | GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE | ANTIBODY | MICROBIOLOGY | MODEL | BIOLOGIC ACTIVITY | LIPOPROTEIN | Recombinant Protein | Expression Vector | GENE | D-BINDING PROTEIN | Haemophilus influenzue | IMMUNOGLOBULIN-D
Journal Article
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