Nature Methods, ISSN 1548-7091, 03/2015, Volume 12, Issue 4, pp. 351 - 356
Speed, single-base sensitivity and long read lengths make nanopores a promising technology for high-throughput sequencing. We evaluated and optimized the...
BASIC LOCAL ALIGNMENT | DNA | GENOME | BIOCHEMICAL RESEARCH METHODS | MULTIPLE ALIGNMENT | Gene Dosage | Algorithms | Neoplasms - genetics | High-Throughput Nucleotide Sequencing - methods | Nanopores | Humans | Chromosome mapping | Nucleotide sequencing | Nanotechnology | Methods | Innovations | DNA sequencing | Nanoscience | Data analysis | Genes | Deoxyribonucleic acid--DNA
BASIC LOCAL ALIGNMENT | DNA | GENOME | BIOCHEMICAL RESEARCH METHODS | MULTIPLE ALIGNMENT | Gene Dosage | Algorithms | Neoplasms - genetics | High-Throughput Nucleotide Sequencing - methods | Nanopores | Humans | Chromosome mapping | Nucleotide sequencing | Nanotechnology | Methods | Innovations | DNA sequencing | Nanoscience | Data analysis | Genes | Deoxyribonucleic acid--DNA
Journal Article
Molecular Ecology Resources, ISSN 1755-098X, 11/2014, Volume 14, Issue 6, pp. 1097 - 1102
Oxford Nanopore's third‐generation single‐molecule sequencing platform promises to decrease costs for reagents and instrumentation. After a 2‐year hiatus...
nanopore sequencing | genomics | next‐generation sequencing | bioinformatics | Next-generation sequencing | Nanopore sequencing | Bioinformatics | Genomics | next-generation sequencing | BIOCHEMISTRY & MOLECULAR BIOLOGY | PLATFORMS | BASIC LOCAL ALIGNMENT | DISCOVERY | EVOLUTIONARY BIOLOGY | HUMAN GENOME | DNA | FRAMEWORK | ECOLOGY | PACIFIC BIOSCIENCES | DNA, Viral - chemistry | Sequence Analysis, DNA - instrumentation | Animals | Exocrine Glands - chemistry | Transcriptome | Bacteriophage lambda - genetics | High-Throughput Nucleotide Sequencing - instrumentation | DNA, Viral - genetics | High-Throughput Nucleotide Sequencing - methods | Sequence Analysis, DNA - methods | Snakes | Nucleotide sequencing | DNA sequencing
nanopore sequencing | genomics | next‐generation sequencing | bioinformatics | Next-generation sequencing | Nanopore sequencing | Bioinformatics | Genomics | next-generation sequencing | BIOCHEMISTRY & MOLECULAR BIOLOGY | PLATFORMS | BASIC LOCAL ALIGNMENT | DISCOVERY | EVOLUTIONARY BIOLOGY | HUMAN GENOME | DNA | FRAMEWORK | ECOLOGY | PACIFIC BIOSCIENCES | DNA, Viral - chemistry | Sequence Analysis, DNA - instrumentation | Animals | Exocrine Glands - chemistry | Transcriptome | Bacteriophage lambda - genetics | High-Throughput Nucleotide Sequencing - instrumentation | DNA, Viral - genetics | High-Throughput Nucleotide Sequencing - methods | Sequence Analysis, DNA - methods | Snakes | Nucleotide sequencing | DNA sequencing
Journal Article
GigaScience, ISSN 2047-217X, 10/2014, Volume 3, Issue 1, p. 22
Background: The MinION (TM) is a new, portable single-molecule sequencer developed by Oxford Nanopore Technologies. It measures four inches in length and is...
Nanopore sequencing | Genomics | DNA | MULTIDISCIPLINARY SCIENCES
Nanopore sequencing | Genomics | DNA | MULTIDISCIPLINARY SCIENCES
Journal Article
Fertility and Sterility, ISSN 0015-0282, 10/2018, Volume 110, Issue 5, pp. 910 - 916.e2
Objective: To determine if a handheld, nanopore-based DNA sequencer can be used for rapid preimplantation genetic screening (PGS). Design: Laboratory study....
handheld nanopore sequencer | Rapid preimplantation genetic screening | aneuploidy screening | onsite assay | VALIDATION | HUMAN BLASTOCYSTS | OBSTETRICS & GYNECOLOGY | ANEUPLOIDY | REPRODUCTIVE BIOLOGY | IN-VITRO FERTILIZATION | TECHNOLOGY | EMBRYOS | PRENATAL-DIAGNOSIS | Usage | Genomic libraries | Analysis | DNA | Nucleotide sequencing | Genetic screening | DNA sequencing
handheld nanopore sequencer | Rapid preimplantation genetic screening | aneuploidy screening | onsite assay | VALIDATION | HUMAN BLASTOCYSTS | OBSTETRICS & GYNECOLOGY | ANEUPLOIDY | REPRODUCTIVE BIOLOGY | IN-VITRO FERTILIZATION | TECHNOLOGY | EMBRYOS | PRENATAL-DIAGNOSIS | Usage | Genomic libraries | Analysis | DNA | Nucleotide sequencing | Genetic screening | DNA sequencing
Journal Article
BMC Infectious Diseases, ISSN 1471-2334, 09/2017, Volume 17, Issue 1, pp. 621 - 9
Background: A simple and accurate molecular diagnostic method for malaria is urgently needed due to the limitations of conventional microscopic examination. In...
Malaria | Plasmodium ovale wallikeri | Plasmodium ovale curtisi | MinION | Diagnosis | LAMP | Nanopore sequencer | PARASITES | INFECTIOUS DISEASES | INFECTIONS | ACCURATE | ASSAY | PERFORMANCE | SENSITIVE DETECTION | MICROSCOPY | IDENTIFICATION | MinION (TM) | POLYMERASE-CHAIN-REACTION | REAL-TIME | Limit of Detection | Molecular Diagnostic Techniques - methods | Nucleic Acid Amplification Techniques - methods | Humans | Molecular Diagnostic Techniques - instrumentation | Plasmodium - genetics | Malaria - diagnosis | Malaria, Vivax - diagnosis | DNA Primers | Nanopores | Malaria, Falciparum - diagnosis | Polymerase Chain Reaction | Nucleic Acid Amplification Techniques - instrumentation | RNA, Ribosomal, 18S | Indonesia | Plasmodium falciparum | Care and treatment | Research | Nucleotide sequencing | DNA sequencing | Vector-borne diseases | Analytical methods | Parasites | Polymerase chain reaction | Amplification | Microscopy | Plasmids | Primers | Diagnostic systems | Mutation | Porosity | Detection limits | rRNA 18S | Species | Methods
Malaria | Plasmodium ovale wallikeri | Plasmodium ovale curtisi | MinION | Diagnosis | LAMP | Nanopore sequencer | PARASITES | INFECTIOUS DISEASES | INFECTIONS | ACCURATE | ASSAY | PERFORMANCE | SENSITIVE DETECTION | MICROSCOPY | IDENTIFICATION | MinION (TM) | POLYMERASE-CHAIN-REACTION | REAL-TIME | Limit of Detection | Molecular Diagnostic Techniques - methods | Nucleic Acid Amplification Techniques - methods | Humans | Molecular Diagnostic Techniques - instrumentation | Plasmodium - genetics | Malaria - diagnosis | Malaria, Vivax - diagnosis | DNA Primers | Nanopores | Malaria, Falciparum - diagnosis | Polymerase Chain Reaction | Nucleic Acid Amplification Techniques - instrumentation | RNA, Ribosomal, 18S | Indonesia | Plasmodium falciparum | Care and treatment | Research | Nucleotide sequencing | DNA sequencing | Vector-borne diseases | Analytical methods | Parasites | Polymerase chain reaction | Amplification | Microscopy | Plasmids | Primers | Diagnostic systems | Mutation | Porosity | Detection limits | rRNA 18S | Species | Methods
Journal Article
GigaScience, ISSN 2047-217X, 2016, Volume 5, Issue 1, p. 4
Background: The miniaturised and portable DNA sequencer MinION (TM) has been released to the scientific community within the framework of an early access...
Long-read sequencing | Microbial diversity | MinION | Nanopore sequencer | 16S rDNA amplicon sequencing | DATABASE | MULTIDISCIPLINARY SCIENCES | BACTERIAL | Reproducibility of Results | Nucleic Acid Amplification Techniques - methods | Species Specificity | DNA, Bacterial - chemistry | Nanopores | Bacteria - genetics | Bacterial Typing Techniques - methods | DNA, Ribosomal - chemistry | DNA, Bacterial - genetics | Bacteria - classification | RNA, Ribosomal, 16S - genetics | Sequence Analysis, DNA - methods | DNA, Ribosomal - genetics
Long-read sequencing | Microbial diversity | MinION | Nanopore sequencer | 16S rDNA amplicon sequencing | DATABASE | MULTIDISCIPLINARY SCIENCES | BACTERIAL | Reproducibility of Results | Nucleic Acid Amplification Techniques - methods | Species Specificity | DNA, Bacterial - chemistry | Nanopores | Bacteria - genetics | Bacterial Typing Techniques - methods | DNA, Ribosomal - chemistry | DNA, Bacterial - genetics | Bacteria - classification | RNA, Ribosomal, 16S - genetics | Sequence Analysis, DNA - methods | DNA, Ribosomal - genetics
Journal Article
Optics and Laser Technology, ISSN 0030-3992, 01/2019, Volume 109, pp. 199 - 211
Our research group is investigating a novel DNA sequencing methodology based on an electrophoretic plasmonic nanopore (EPIGeneS) biochip system used to control...
Electrophoretic DNA translocation | Fractal nanopores | Plasmonic nanopores | Surface enhance Raman spectroscopy | DNA sequencing | PHYSICS, APPLIED | DNA | OPTICS
Electrophoretic DNA translocation | Fractal nanopores | Plasmonic nanopores | Surface enhance Raman spectroscopy | DNA sequencing | PHYSICS, APPLIED | DNA | OPTICS
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1802, pp. 155 - 162
Nanopore sequencing, a paradigm change in sequencing technologies, offers a new cost-effective and scalable platform for HLA genotyping. Among the new...
Full-length | MinION | Genotyping | HLA | Nanopore sequencing | Gene Library | Humans | Histocompatibility Antigens Class I - genetics | High-Throughput Nucleotide Sequencing - instrumentation | Genotyping Techniques - methods | High-Throughput Nucleotide Sequencing - methods | Nanopores
Full-length | MinION | Genotyping | HLA | Nanopore sequencing | Gene Library | Humans | Histocompatibility Antigens Class I - genetics | High-Throughput Nucleotide Sequencing - instrumentation | Genotyping Techniques - methods | High-Throughput Nucleotide Sequencing - methods | Nanopores
Journal Article
GigaScience, ISSN 2047-217X, 03/2015, Volume 4, Issue 1, pp. 12 - 12
Background: The MinION (TM) nanopore sequencer was recently released to a community of alpha-testers for evaluation using a variety of sequencing applications....
Poxvirus | Amplicon | Whole genome sequencing | Escherichia coli | Long-read sequencing | MinION | Oxford Nanopore | Pathogen detection | Nanopore | NGS | SINGLE | MULTIDISCIPLINARY SCIENCES | MinION (TM) | DISCRIMINATION | DNA | Classification - methods | Viruses - genetics | Nanopores | Bacteria - genetics | Sequence Analysis, DNA - methods
Poxvirus | Amplicon | Whole genome sequencing | Escherichia coli | Long-read sequencing | MinION | Oxford Nanopore | Pathogen detection | Nanopore | NGS | SINGLE | MULTIDISCIPLINARY SCIENCES | MinION (TM) | DISCRIMINATION | DNA | Classification - methods | Viruses - genetics | Nanopores | Bacteria - genetics | Sequence Analysis, DNA - methods
Journal Article
JOURNAL OF MEDICAL MICROBIOLOGY, ISSN 0022-2615, 08/2019, Volume 68, Issue 8, pp. 1219 - 1226
Purpose. The new third-generation sequencing platform MinION is an attractive maintenance-free and disposable portable tool that can perform long-read and...
nanopore sequencer | 16S rRNA | MinION | MICROBIOLOGY | bloodstream infection | TIME | CEPHALOSPORINS | extended-spectrum beta-lactamase | DNA EXTRACTION | MALDI-TOF MS | EXPANSION | RESISTANCE | CULTURES | RAPID DETECTION | EPIDEMIOLOGY | ENTEROBACTERIACEAE | PATHOGENS
nanopore sequencer | 16S rRNA | MinION | MICROBIOLOGY | bloodstream infection | TIME | CEPHALOSPORINS | extended-spectrum beta-lactamase | DNA EXTRACTION | MALDI-TOF MS | EXPANSION | RESISTANCE | CULTURES | RAPID DETECTION | EPIDEMIOLOGY | ENTEROBACTERIACEAE | PATHOGENS
Journal Article
SCIENCE, ISSN 0036-8075, 02/2016, Volume 351, Issue 6275, pp. 800 - 801
Journal Article
GENOME BIOLOGY, ISSN 1474-760X, 07/2019, Volume 20, Issue 1, pp. 134 - 9
We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules....
Copy number variation | Nanopore sequencing | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | Long-read sequencing | TIME | Read-counting applications | GENOME
Copy number variation | Nanopore sequencing | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | Long-read sequencing | TIME | Read-counting applications | GENOME
Journal Article
Journal of medical microbiology, ISSN 0022-2615, 11/2018, Volume 67, Issue 11, pp. 1589 - 1595
Purpose. Bloodstream infections are major causes of morbidity and mortality that lead to prolonged hospital stays and higher medical costs. In this study, we...
16S rRNA | ITS | MinION | bloodstream infection | candidemia | sequencing | BACTERIA | CANDIDA | ESCHERICHIA-COLI | MICROBIOLOGY | STREAM INFECTIONS | DNA EXTRACTION | FILMARRAY | FLIGHT MASS-SPECTROMETRY | POLYMERASE-CHAIN-REACTION | DESORPTION IONIZATION-TIME | SPECIES-LEVEL | DNA, Fungal - genetics | Humans | Fungi - isolation & purification | DNA, Fungal - isolation & purification | Fungemia - blood | Nanopores | Fungi - genetics | Polymerase Chain Reaction - methods | Bacteremia - microbiology | Sequence Analysis, DNA - instrumentation | Blood Culture - methods | Bacteremia - blood | Bacteria - classification | Fungi - pathogenicity | Candida - genetics | DNA, Bacterial - isolation & purification | Bacteremia - diagnosis | Sequence Analysis, DNA - statistics & numerical data | Fungemia - microbiology | Bacteria - genetics | Candida - isolation & purification | Fungemia - diagnosis | Blood Culture - instrumentation | Bacteria - isolation & purification | DNA, Bacterial - genetics | Candida - pathogenicity | Escherichia coli - genetics | Escherichia coli - isolation & purification | Bacteria - pathogenicity | High-Throughput Nucleotide Sequencing | Sequence Analysis, DNA - economics | Sequence Analysis, DNA - methods
16S rRNA | ITS | MinION | bloodstream infection | candidemia | sequencing | BACTERIA | CANDIDA | ESCHERICHIA-COLI | MICROBIOLOGY | STREAM INFECTIONS | DNA EXTRACTION | FILMARRAY | FLIGHT MASS-SPECTROMETRY | POLYMERASE-CHAIN-REACTION | DESORPTION IONIZATION-TIME | SPECIES-LEVEL | DNA, Fungal - genetics | Humans | Fungi - isolation & purification | DNA, Fungal - isolation & purification | Fungemia - blood | Nanopores | Fungi - genetics | Polymerase Chain Reaction - methods | Bacteremia - microbiology | Sequence Analysis, DNA - instrumentation | Blood Culture - methods | Bacteremia - blood | Bacteria - classification | Fungi - pathogenicity | Candida - genetics | DNA, Bacterial - isolation & purification | Bacteremia - diagnosis | Sequence Analysis, DNA - statistics & numerical data | Fungemia - microbiology | Bacteria - genetics | Candida - isolation & purification | Fungemia - diagnosis | Blood Culture - instrumentation | Bacteria - isolation & purification | DNA, Bacterial - genetics | Candida - pathogenicity | Escherichia coli - genetics | Escherichia coli - isolation & purification | Bacteria - pathogenicity | High-Throughput Nucleotide Sequencing | Sequence Analysis, DNA - economics | Sequence Analysis, DNA - methods
Journal Article
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Full Text
Pores for thought: preimplantation genetic testing using a nanopore-based DNA sequencer
Fertility and Sterility, ISSN 0015-0282, 10/2018, Volume 110, Issue 5, pp. 853 - 855
Journal Article
15.
Full Text
Real-time DNA sequencing in the antarctic dry valleys using the Oxford nanopore sequencer
Journal of Biomolecular Techniques, ISSN 1524-0215, 04/2017, Volume 28, Issue 1, pp. 2 - 7
The ability to sequence DNA outside of the laboratory setting has enabled novel research questions to be addressed in the field in diverse areas, ranging from...
MinION | Antarctica | Nanopore sequencing | Extremophiles | Antarctic Regions | Sequence Analysis, DNA - instrumentation | Reference Standards | Environmental Microbiology | Bacteriophage lambda - genetics | High-Throughput Nucleotide Sequencing - instrumentation | Desert Climate | Molecular Typing - instrumentation | extremophiles | Short Communication
MinION | Antarctica | Nanopore sequencing | Extremophiles | Antarctic Regions | Sequence Analysis, DNA - instrumentation | Reference Standards | Environmental Microbiology | Bacteriophage lambda - genetics | High-Throughput Nucleotide Sequencing - instrumentation | Desert Climate | Molecular Typing - instrumentation | extremophiles | Short Communication
Journal Article
Antimicrobial Agents and Chemotherapy, ISSN 0066-4804, 11/2016, Volume 60, Issue 11, pp. 6780 - 6786
Two A/C incompatibility group (IncA/C family) plasmids from the 1960s have been sequenced and classified into the A/C2 type 1 group. R16a and IP40a contain...
ELEMENT | GENES | SALMONELLA | MICROBIOLOGY | PHARMACOLOGY & PHARMACY | BACTERIAL | IDENTIFICATION | COMPLETE NUCLEOTIDE-SEQUENCE | ANTIMICROBIAL RESISTANCE | NANOPORE SEQUENCER | GENOME | STREPTOMYCIN | Conjugation, Genetic | Genome, Bacterial | Plasmids - history | Escherichia coli - drug effects | History, 20th Century | Genomic Islands | Plasmids - metabolism | Sequence Analysis, DNA | DNA Transposable Elements | Automation, Laboratory | DNA, Bacterial - genetics | beta-Lactamases - genetics | Escherichia coli - genetics | Plasmids - chemistry | Anti-Bacterial Agents - pharmacology | Escherichia coli - growth & development | Drug Resistance, Multiple, Bacterial - genetics
ELEMENT | GENES | SALMONELLA | MICROBIOLOGY | PHARMACOLOGY & PHARMACY | BACTERIAL | IDENTIFICATION | COMPLETE NUCLEOTIDE-SEQUENCE | ANTIMICROBIAL RESISTANCE | NANOPORE SEQUENCER | GENOME | STREPTOMYCIN | Conjugation, Genetic | Genome, Bacterial | Plasmids - history | Escherichia coli - drug effects | History, 20th Century | Genomic Islands | Plasmids - metabolism | Sequence Analysis, DNA | DNA Transposable Elements | Automation, Laboratory | DNA, Bacterial - genetics | beta-Lactamases - genetics | Escherichia coli - genetics | Plasmids - chemistry | Anti-Bacterial Agents - pharmacology | Escherichia coli - growth & development | Drug Resistance, Multiple, Bacterial - genetics
Journal Article
FEBS Open Bio, ISSN 2211-5463, 03/2019, Volume 9, Issue 3, pp. 548 - 557
Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic treatment, which significantly improves patient outcomes. 16S...
nanopore sequencer | 16S rRNA | MinION | bacterial identification | direct PCR | PHYLOGENETIC ANALYSIS | BIOCHEMISTRY & MOLECULAR BIOLOGY | DIVERSITY | ENVIRONMENTAL-SAMPLES | CLINICAL MICROBIOLOGY | PATHOGENS
nanopore sequencer | 16S rRNA | MinION | bacterial identification | direct PCR | PHYLOGENETIC ANALYSIS | BIOCHEMISTRY & MOLECULAR BIOLOGY | DIVERSITY | ENVIRONMENTAL-SAMPLES | CLINICAL MICROBIOLOGY | PATHOGENS
Journal Article
Malaria Journal, ISSN 1475-2875, 05/2018, Volume 17, Issue 1, pp. 217 - 11
Background: The recent spread of artemisinin (ART)-resistant Plasmodium falciparum represents an emerging global threat to public health. In Southeast Asia,...
Artemisinin resistance | Plasmodium falciparum | Malaria | MinION | kelch 13 | LAMP | Nanopore sequencer | INFECTIOUS DISEASES | ASSAY | MEDIATED ISOTHERMAL AMPLIFICATION | DENGUE VIRUS SEROTYPES | ELIMINATION | HAEMOPHILUS-INFLUENZAE | TROPICAL MEDICINE | MinION (TM) | POLYMERASE-CHAIN-REACTION | MALARIA DIAGNOSIS | RAPID DETECTION | PARASITOLOGY | Malaria, Falciparum - parasitology | Nucleic Acid Amplification Techniques - methods | Humans | Plasmodium falciparum - genetics | Plasmodium falciparum - isolation & purification | Malaria, Falciparum - classification | Mutation | Nanopores | Protozoan Proteins - genetics | Indonesia | Usage | Gene mutations | Causes of | Genetic aspects | Nucleotide sequencing | Research | Risk factors | DNA sequencing | Protocols | Methodology | Laboratories | Artemisinin | Erythrocytes | Developing countries | Procedures | Parasites | Nucleotides | Developing countries--LDCs | Sequencing | Public health | Deoxyribonucleic acid--DNA | Vector-borne diseases | Evaluation | Mutations | Human diseases | Nucleotide sequence | Health risks | Polymerase chain reaction | Plasmids | DNA | Detection
Artemisinin resistance | Plasmodium falciparum | Malaria | MinION | kelch 13 | LAMP | Nanopore sequencer | INFECTIOUS DISEASES | ASSAY | MEDIATED ISOTHERMAL AMPLIFICATION | DENGUE VIRUS SEROTYPES | ELIMINATION | HAEMOPHILUS-INFLUENZAE | TROPICAL MEDICINE | MinION (TM) | POLYMERASE-CHAIN-REACTION | MALARIA DIAGNOSIS | RAPID DETECTION | PARASITOLOGY | Malaria, Falciparum - parasitology | Nucleic Acid Amplification Techniques - methods | Humans | Plasmodium falciparum - genetics | Plasmodium falciparum - isolation & purification | Malaria, Falciparum - classification | Mutation | Nanopores | Protozoan Proteins - genetics | Indonesia | Usage | Gene mutations | Causes of | Genetic aspects | Nucleotide sequencing | Research | Risk factors | DNA sequencing | Protocols | Methodology | Laboratories | Artemisinin | Erythrocytes | Developing countries | Procedures | Parasites | Nucleotides | Developing countries--LDCs | Sequencing | Public health | Deoxyribonucleic acid--DNA | Vector-borne diseases | Evaluation | Mutations | Human diseases | Nucleotide sequence | Health risks | Polymerase chain reaction | Plasmids | DNA | Detection
Journal Article
SCIENCE, ISSN 0036-8075, 02/2014, Volume 343, Issue 6173, pp. 829 - 830