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biochemistry & molecular biology (127) 127
glycosylation (122) 122
animals (104) 104
humans (95) 95
pngase f (85) 85
index medicus (64) 64
molecular sequence data (62) 62
research article (49) 49
proteins (46) 46
amino acid sequence (45) 45
pngase f, peptide n-glycosidase f (45) 45
glycoproteins (44) 44
deglycosylation (42) 42
biophysics (39) 39
cell line (38) 38
mice (38) 38
pngase (37) 37
cell biology (36) 36
glycoproteins - chemistry (32) 32
carbohydrate sequence (30) 30
identification (30) 30
er, endoplasmic reticulum (28) 28
mass spectrometry (28) 28
peptide-n4- asparagine amidase (25) 25
endoplasmic-reticulum (24) 24
glycoproteins - metabolism (23) 23
oligosaccharides (23) 23
peptide-n4- asparagine amidase - metabolism (23) 23
enzymes (22) 22
expression (22) 22
glycans (22) 22
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pngase-f (22) 22
cricetinae (20) 20
endo h, endoglycosidase h (20) 20
peptides (20) 20
recombinant proteins - metabolism (20) 20
biochemical research methods (19) 19
purification (19) 19
protein binding (18) 18
electrophoresis, polyacrylamide gel (17) 17
recombinant proteins - chemistry (17) 17
substrate specificity (17) 17
mass-spectrometry (16) 16
n-linked glycosylation (16) 16
polysaccharides - chemistry (16) 16
polysaccharides - metabolism (16) 16
rats (16) 16
analysis (15) 15
article (15) 15
biotechnology & applied microbiology (15) 15
hrp, horseradish peroxidase (15) 15
protein structure, tertiary (15) 15
amidohydrolases - metabolism (14) 14
binding sites (14) 14
chromatography, high pressure liquid (14) 14
n-glycosylation (14) 14
protein (14) 14
protein processing, post-translational (14) 14
protein transport (14) 14
sequence homology, amino acid (14) 14
carbohydrates (13) 13
cells (13) 13
cells, cultured (13) 13
endoplasmic reticulum (13) 13
gfp, green fluorescent protein (13) 13
glycoside hydrolases - metabolism (13) 13
mutation (13) 13
peptide (13) 13
peptide-n4- asparagine amidase - chemistry (13) 13
polysaccharides (13) 13
base sequence (12) 12
cleavage (12) 12
dmem, dulbecco's modified eagle's medium (12) 12
gene (12) 12
n-glycan (12) 12
polysaccharides - analysis (12) 12
recombinant proteins - genetics (12) 12
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amidase (11) 11
cattle (11) 11
cell membrane - metabolism (11) 11
de-n-glycosylation (11) 11
endoplasmic reticulum - metabolism (11) 11
life sciences (11) 11
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carbohydrate conformation (10) 10
engase (10) 10
gene expression (10) 10
lectins (10) 10
oligosaccharides - chemistry (10) 10
protein conformation (10) 10
recombinant proteins (10) 10
sequence alignment (10) 10
transfection (10) 10
tunicamycin (10) 10
yeast (10) 10
binding (9) 9
blotting, western (9) 9
female (9) 9
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Bioscience, Biotechnology, and Biochemistry, ISSN 0916-8451, 07/2018, Volume 82, Issue 7, pp. 1172 - 1175
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 02/2013, Volume 12, Issue 2, pp. 573 - 584
Journal Article
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, ISSN 1000-3061, 03/2018, Volume 34, Issue 3, pp. 421 - 428
Journal Article
International Dairy Journal, ISSN 0958-6946, 10/2014, Volume 38, Issue 2, pp. 169 - 173
Healthier dairy products can be obtained by reducing fat content, but removal of fat from fermented milk products causes a reduction in gel firmness. To... 
YOGURT | PNGASE-F | FOOD SCIENCE & TECHNOLOGY | Enzymes | Milk
Journal Article
Biochemical Journal, ISSN 0264-6021, 11/2006, Volume 400, Issue 1, pp. 33 - 41
The endoplasmic-reticulum-associated degradation of misfolded (glyco)proteins ensures that only functional, correctly folded proteins exit from the endoplasmic... 
Peptide:N-glycanase (PNGase) | N-glycan | Non-lysosomal degradation | α-mannosidase | Cytosol | Free oligosaccharide | RAT-LIVER | non-lysosomal degradation | cytosol | BIOCHEMISTRY & MOLECULAR BIOLOGY | FULL-LENGTH CDNA | alpha-mannosidase | SUBSTRATE-SPECIFICITY | free oligosaccharide | peptide : N-glycanase (PNGase) | PEPTIDE-N-GLYCANASE | HEPG2 CELLS | POLYMANNOSE-TYPE OLIGOSACCHARIDES | ENDOPLASMIC-RETICULUM | HEN OVIDUCT | EXPRESSION | JAPANESE-QUAIL OVIDUCT | Immunohistochemistry | Membrane Glycoproteins - metabolism | Oligopeptides | Humans | alpha-Mannosidase - metabolism | Cytosol - drug effects | Peptides - genetics | alpha-Mannosidase - antagonists & inhibitors | Green Fluorescent Proteins - genetics | Mannosidases - metabolism | Chromatography, High Pressure Liquid | Recombinant Fusion Proteins - metabolism | Cytosol - enzymology | Peptides - metabolism | Transfection | Cobalt - pharmacology | RNA Interference | Oligosaccharides - chemistry | Mannosidases - genetics | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | Swainsonine - pharmacology | Cell Line | Green Fluorescent Proteins - metabolism | Alkaloids - pharmacology | Mannosidases - antagonists & inhibitors | Oligosaccharides - metabolism | Blotting, Western | Gene Expression Regulation, Enzymologic | Recombinant Fusion Proteins - genetics | Cytosol - metabolism | alpha-Mannosidase - genetics | EGFP, enhanced green fluorescent protein | KIF, kifunensine | RNAi, RNA interference | 2-AA, 2-aminobenzoic acid | PNGase, peptide:N-glycanase | DMM, deoxymannojirimycin | siRNA, small interfering RNA | pNP-α-Man, p-nitrophenyl-α-D-mannoside | peptide:N-glycanase (PNGase) | PA, pyridylamino | MALDI–TOF MS, matrix-assisted laser desorption ionization–time-of-flight MS | ERAD, ER-associated degradation | RNase B, ribonuclease B | ENGase, endo-β-N-acetylglucosaminidase | SW, swainsonine | ER, endoplasmic reticulum | PDI, protein disulfide-isomerase | GAPDH, glyceraldehyde-3-phosphate dehydrogenase | TFA, trifluoroacetic acid
Journal Article
Bioscience, Biotechnology, and Biochemistry, ISSN 0916-8451, 07/2019, Volume 83, Issue 7, pp. 1310 - 1314
Free N-glycans (FNGs) are ubiquitous in growing plants. Further, acidic peptide:N-glycanase is believed to be involved in the production of plant complex-type... 
xylem sap | solanum lycopersicum | deglycosylation | PNGase | Free N-glycan | Free -glycan | MOLECULAR CHARACTERIZATION | ALPHA-MANNOSIDASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | FOOD SCIENCE & TECHNOLOGY | MEMBER | SEEDS | SUBSTRATE-SPECIFICITY | AMIDASE | FRUIT | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PURIFICATION | CHEMISTRY, APPLIED | EXPRESSION
Journal Article
Journal of Chromatography A, ISSN 0021-9673, 12/2013, Volume 1322, pp. 54 - 61
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid,... 
PNGase F | Deglycosylation | Enzyme microreactor | Oriented immobilization | Monolith | CHEMISTRY, ANALYTICAL | GLYCANS | TRYPSIN | BIOCHEMICAL RESEARCH METHODS | GLYCOPROTEINS | MICROREACTOR | RELEASE | CHROMATOGRAPHY | MASS-SPECTROMETRY | POROUS POLYMER MONOLITH | REACTOR | PEPTIDE-N-4-(N-ACETYL-BETA-D-GLUCOSAMINYL)ASPARAGINE AMIDASE
Journal Article
Journal Article
Carbohydrate Research, ISSN 0008-6215, 01/2019, Volume 472, pp. 58 - 64
PNGases are crucial targets and valuable tools in analyzing asparagine-linked carbohydrate moieties (N-glycans) of glycoproteins. Activity tests of PNGases... 
Colorimetric assay | N-glycan | Kinetic analysis | PNGase | WST-1 | CELLS | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, ORGANIC | IDENTIFICATION | N-GLYCOSIDASE-F | AMIDASE | CLONING | RECOMBINANT PROTEINS | PLANTS | CHEMISTRY, APPLIED | GLYCANASE | EXPRESSION | TOOLS
Journal Article
Mass Spectrometry Letters, ISSN 2233-4203, 03/2017, Volume 8, Issue 1, pp. 14 - 17
Journal Article
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2019, Volume 9, Issue 1, pp. 4865 - 13
Enzyme immobilization is widely applied in biocatalysis to improve stability and facilitate recovery and reuse of enzymes. However, high cost of supporting... 
3-DIMENSIONAL STRUCTURE | MICROWAVE-ASSISTED DEGLYCOSYLATION | IN-VITRO | DISPLAY | PNGASE-F | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | PROTEIN EXPRESSION | GLYCANS | MALDI-MS | MASS-SPECTROMETRY | Deglycosylation | Enzymes | Capsid protein | Peptides | Centrifugation | Phages | Immobilization | Industrial applications | Nanoparticles | Capsids | Self-assembly | Catalysis | N-glycosidase | Binding sites
Journal Article
Journal of Visualized Experiments, ISSN 1940-087X, 06/2018, Volume 2018, Issue 136
In recent years, the carbohydrate moieties of plants have received considerable attention, as they are a potential source of cross-reactive, allergy-provoking... 
Issue 136 | MALDI-TOF | Core fucosylation | Biochemistry | PNGase H | N-linked glycosylation | Raphanus sativus | HPLC | PROTEIN | MULTIDISCIPLINARY SCIENCES | GLYCOSYLATION | RELEASE | AMIDASE | core fucosylation
Journal Article