X
Search Filters
Format Format
Subjects Subjects
Subjects Subjects
X
Sort by Item Count (A-Z)
Filter by Count
glycosylation (109) 109
biochemistry & molecular biology (108) 108
animals (95) 95
humans (90) 90
pngase f (85) 85
index medicus (57) 57
molecular sequence data (55) 55
research article (46) 46
pngase f, peptide n-glycosidase f (45) 45
proteins (44) 44
amino acid sequence (43) 43
glycoproteins (39) 39
biophysics (37) 37
deglycosylation (37) 37
cell biology (35) 35
cell line (35) 35
mice (34) 34
er, endoplasmic reticulum (27) 27
glycoproteins - chemistry (27) 27
identification (27) 27
mass spectrometry (25) 25
carbohydrate sequence (24) 24
oligosaccharides (22) 22
pngase-f (22) 22
cricetinae (20) 20
endo h, endoglycosidase h (20) 20
expression (20) 20
glycoproteins - metabolism (20) 20
enzymes (19) 19
glycoprotein (19) 19
recombinant proteins - metabolism (19) 19
biochemical research methods (18) 18
peptide-n4- asparagine amidase (18) 18
peptide-n4- asparagine amidase - metabolism (18) 18
peptides (18) 18
protein binding (18) 18
glycans (17) 17
recombinant proteins - chemistry (17) 17
electrophoresis, polyacrylamide gel (16) 16
endoplasmic-reticulum (16) 16
mass-spectrometry (16) 16
polysaccharides - metabolism (16) 16
rats (16) 16
biotechnology & applied microbiology (15) 15
n-linked glycosylation (15) 15
polysaccharides - chemistry (15) 15
article (14) 14
binding sites (14) 14
hrp, horseradish peroxidase (14) 14
n-glycosylation (14) 14
pngase (14) 14
protein processing, post-translational (14) 14
purification (14) 14
sequence homology, amino acid (14) 14
analysis (13) 13
chromatography, high pressure liquid (13) 13
gfp, green fluorescent protein (13) 13
protein (13) 13
protein structure, tertiary (13) 13
carbohydrates (12) 12
dmem, dulbecco's modified eagle's medium (12) 12
gene (12) 12
polysaccharides (12) 12
protein transport (12) 12
cattle (11) 11
cell membrane - metabolism (11) 11
cells (11) 11
life sciences (11) 11
molecular weight (11) 11
mutation (11) 11
recombinant proteins - genetics (11) 11
sequence (11) 11
amidohydrolases - metabolism (10) 10
base sequence (10) 10
cells, cultured (10) 10
cleavage (10) 10
endoplasmic reticulum (10) 10
gene expression (10) 10
n-glycan (10) 10
peptide (10) 10
peptide-n4- asparagine amidase - chemistry (10) 10
polysaccharides - analysis (10) 10
recombinant proteins (10) 10
sequence alignment (10) 10
substrate specificity (10) 10
tunicamycin (10) 10
binding (9) 9
glycan (9) 9
glycoside hydrolases - metabolism (9) 9
lectins (9) 9
molecular-cloning (9) 9
amidase (8) 8
blotting, western (8) 8
carbohydrate conformation (8) 8
cell line, tumor (8) 8
chemistry, analytical (8) 8
cho cells (8) 8
endoglycosidase (8) 8
hydrolysis (8) 8
mab, monoclonal antibody (8) 8
more...
Language Language
Publication Date Publication Date
Click on a bar to filter by decade
Slide to change publication date range


Scientific Reports, ISSN 2045-2322, 12/2019, Volume 9, Issue 1, pp. 4865 - 13
Enzyme immobilization is widely applied in biocatalysis to improve stability and facilitate recovery and reuse of enzymes. However, high cost of supporting... 
3-DIMENSIONAL STRUCTURE | MICROWAVE-ASSISTED DEGLYCOSYLATION | IN-VITRO | DISPLAY | PNGASE-F | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | PROTEIN EXPRESSION | GLYCANS | MALDI-MS | MASS-SPECTROMETRY | Deglycosylation | Enzymes | Capsid protein | Peptides | Centrifugation | Phages | Immobilization | Industrial applications | Nanoparticles | Capsids | Self-assembly | Catalysis | N-glycosidase | Binding sites
Journal Article
Journal of Chromatography A, ISSN 0021-9673, 12/2013, Volume 1322, pp. 54 - 61
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid,... 
PNGase F | Deglycosylation | Enzyme microreactor | Oriented immobilization | Monolith | CHEMISTRY, ANALYTICAL | GLYCANS | TRYPSIN | BIOCHEMICAL RESEARCH METHODS | GLYCOPROTEINS | MICROREACTOR | RELEASE | CHROMATOGRAPHY | MASS-SPECTROMETRY | POROUS POLYMER MONOLITH | REACTOR | PEPTIDE-N-4-(N-ACETYL-BETA-D-GLUCOSAMINYL)ASPARAGINE AMIDASE
Journal Article
International Dairy Journal, ISSN 0958-6946, 10/2014, Volume 38, Issue 2, pp. 169 - 173
Healthier dairy products can be obtained by reducing fat content, but removal of fat from fermented milk products causes a reduction in gel firmness. To... 
YOGURT | PNGASE-F | FOOD SCIENCE & TECHNOLOGY | Enzymes | Milk
Journal Article
Plant Biotechnology Journal, ISSN 1467-7644, 09/2012, Volume 10, Issue 7, pp. 773 - 782
Summary Application of tools of molecular biology and genomics is increasingly leading towards the development of recombinant protein‐based biologics. As such,... 
PNGase F | recombinant proteins | deglycosylation | malaria vaccine candidate Pfs48/45 | plant transient expression | N‐linked glycosylation | Deglycosylation | N-linked glycosylation | Malaria vaccine candidate Pfs48/45 | Recombinant proteins | Plant transient expression | 45 | ANTIGEN PFS48/45 | MONOCLONAL-ANTIBODY | N-GLYCOSYLATION | TUNICAMYCIN | malaria vaccine candidate Pfs48 | VECTOR | PLANT SCIENCES | CHALLENGE | CLONING | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PLASMODIUM-FALCIPARUM | SEQUENCE | RECEPTORS | Enzyme-Linked Immunosorbent Assay | Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase - isolation & purification | Tobacco - metabolism | Bacterial Toxins - immunology | Electrophoresis, Polyacrylamide Gel | Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase - metabolism | Antigens, Bacterial - immunology | Solubility | Glycosylation | Recombinant Proteins - biosynthesis | Blotting, Western | Polysaccharides - metabolism | Biotechnology - methods | Protozoan Proteins - metabolism | Peptide Mapping | Mass Spectrometry | Plants, Genetically Modified | Protein Binding | Bacteria - enzymology | Plasmodium falciparum - metabolism | Protozoan Proteins - chemistry | Antibodies, Monoclonal - immunology | Plasmodium falciparum | Enzymes | Polysaccharides | Peptides | Malaria | Malaria vaccine | Genomics | Monoclonal antibodies | Antigenic determinants
Journal Article
Journal Article
Analytical Biochemistry, ISSN 0003-2697, 8/2012, Volume 427, Issue 1, pp. 33 - 35
Common de-N-glycosylation protocols usually require a lengthy incubation time. Although pressure cycling-technology or scientific microwave reactors can... 
PNGase F | De-N-glycosylation | glycoprotein | N-glycan | domestic microwave
Journal Article
Protein Expression and Purification, ISSN 1046-5928, 05/2014, Volume 97, pp. 17 - 22
Journal Article
Molecular Biotechnology, ISSN 1073-6085, 8/2015, Volume 57, Issue 8, pp. 735 - 745
Journal Article
BIOENGINEERED, ISSN 2165-5979, 09/2013, Volume 4, Issue 5, pp. 338 - 342
At present, several eukaryotic expression systems including yeast, insect and mammalian cells and plants are used for the production of recombinant proteins.... 
ANTIBODIES | PNGase F | recombinant proteins | ANTIGEN PFS48/45 | ABERRANT N-GLYCOSYLATION | plant transient expression | TUNICAMYCIN | CHALLENGE | deglycosylation | malaria vaccine candidate Pfs48/45 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PLASMODIUM-FALCIPARUM | CELL-MIGRATION | MICE | N-linked glycosylation | SUBUNIT
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 02/2013, Volume 12, Issue 2, pp. 573 - 584
Journal Article
Journal Article
Biochemical Journal, ISSN 0264-6021, 08/2005, Volume 390, Issue 1, pp. 263 - 271
The Lass (longevity-assurance homologue) family members, which are highly conserved among eukaryotes, function in ceramide synthesis. In the mouse, there are... 
Sphingolipid | Topology | Ceramide synthase | Longevity assurance homologue (Lass) | N-glycosylation | RAT-LIVER | CYTOSOLIC SURFACE | sphingolipid | longevity assurance homologue (Lass) | topology | BIOCHEMISTRY & MOLECULAR BIOLOGY | ceramide synthase | MEMBRANE-PROTEIN | ASSURANCE GENE LAG1 | SPHINGOLIPIDS | YEAST | GLUCOSYLCERAMIDE SYNTHESIS | ENDOPLASMIC-RETICULUM | DIFFERENTIATION | Multigene Family - genetics | Amino Acid Sequence | Cell Line | Multigene Family - physiology | Oxidoreductases - metabolism | Membrane Proteins - genetics | Humans | Gene Expression Regulation - physiology | Molecular Sequence Data | Substrate Specificity | Phylogeny | RNA, Messenger - metabolism | Intracellular Membranes - chemistry | Ceramides - biosynthesis | Tissue Distribution | Endoplasmic Reticulum - chemistry | Animals | Cloning, Molecular | Membrane Proteins - metabolism | Mice | Endo Hf, endoglycosidase H | CHO, Chinese hamster ovary | Lass, longevity-assurance homologue | Lag, longevity-assurance gene | HEK, human embryonic kidney | HOX, homeobox | Lag1p | EST, expressed sequence tag | TRH, TRAM homologue | PNGase F, N-glycosidase F | Lip1p, Lag1p | TLC domain, TRAM | GFP, green fluorescent protein | ORF, open reading frame | TRAM, translocating chain-associating membrane | HA, haemagglutinin | LAG | CLN8 (ceroid-lipofuscinoses, neuronal 8) domain | TM1 (etc.), transmembrane segment 1 (etc.) | ER, endoplasmic reticulum | FB1, fumonisin B1 | Lac1p-interacting protein
Journal Article
mAbs, ISSN 1942-0862, 07/2015, Volume 7, Issue 4, pp. 732 - 742
To monitor the Fc glycosylation of therapeutic immunoglobulin G in bioprocess development, product characterization and release analytics, reliable techniques... 
IgG glycosylation | LC-MS | method comparison | HILIC-UPLC | mass spectrometry | ESI-MS | MALDI-MS | monoclonal antibody (mAb) | glycan analysis | Glycan analysis | Monoclonal antibody (mAb) | Method comparison | Mass spectrometry | ANTIBODIES | MEDICINE, RESEARCH & EXPERIMENTAL | 2-aminobenzamide | proteolytic enzyme like protease from Streptococcus pyrogenes | PNGase F | IgG | GLYCOPROTEINS | 2-AB | N-GLYCOSYLATION | capillary electrophoresis | high-performance anion exchange chromatography with pulsed amperometric detection | IdeS protease | GLYCOPEPTIDES | immunoglobulin G | OLIGOSACCHARIDES | ACID | liquid chromatography-mass spectrometry | fragment crystallizable | Fab | MALDI | GLYCANS | HILIC-UHPLC | Peptide-N-Glycosidase F | HPAEC-PAD | PGC-MS | IDENTIFICATION | total ion chromatogram | mAb | TIC | RP-HPLC | hydrophilic interaction liquid chromatography-ultra high performance liquid chromatography | fragment antigen-binding | porous graphitized carbon chromatography- mass spectrometry | electrospray ionization-mass spectrometry | reversed phase high performance liquid chromatography | monoclonal antibody | GLYCOMICS | matrix assisted laser desorption ionization | LCMS | Cricetinae | Animals | Cricetulus | Humans | Immunoglobulin G - chemistry | Recombinant Proteins - chemistry | Sialic Acids - analysis | Immunoglobulin Fc Fragments - chemistry | Glycosylation | Antibodies, Monoclonal - chemistry | CHO Cells
Journal Article
Biochemical Journal, ISSN 0264-6021, 12/2008, Volume 416, Issue 2, pp. 297 - 305
Disease-related PrPSc [pathogenic PrP (prion protein)] is classically distinguished from its normal cellular precursor, PrPC(cellular PrP) by its detergent...