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biochemistry & molecular biology (61) 61
glycosylation (53) 53
animals (48) 48
humans (46) 46
pngase f, peptide n-glycosidase f (41) 41
research article (39) 39
index medicus (35) 35
pngase f (34) 34
amino acid sequence (27) 27
molecular sequence data (27) 27
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proteins (21) 21
er, endoplasmic reticulum (20) 20
mice (20) 20
deglycosylation (18) 18
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mass spectrometry (16) 16
cell biology (15) 15
endo h, endoglycosidase h (15) 15
expression (14) 14
protein binding (13) 13
cricetinae (11) 11
gfp, green fluorescent protein (11) 11
hrp, horseradish peroxidase (11) 11
identification (11) 11
protein transport (11) 11
rats (11) 11
dmem, dulbecco's modified eagle's medium (10) 10
mutation (10) 10
n-linked glycosylation (10) 10
peptide-n4- asparagine amidase - metabolism (10) 10
peptides (10) 10
polysaccharides - metabolism (10) 10
cells (9) 9
electrophoresis, polyacrylamide gel (9) 9
oligosaccharides (9) 9
pngase-f (9) 9
protein structure, tertiary (9) 9
sequence homology, amino acid (9) 9
biochemical research methods (8) 8
biophysics (8) 8
cell membrane - metabolism (8) 8
glycoproteins - chemistry (8) 8
mass-spectrometry (8) 8
n-glycosylation (8) 8
polysaccharides (8) 8
protein (8) 8
article (7) 7
base sequence (7) 7
cells, cultured (7) 7
enzymes (7) 7
glycans (7) 7
life sciences (7) 7
mab, monoclonal antibody (7) 7
protein processing, post-translational (7) 7
purification (7) 7
recombinant proteins - metabolism (7) 7
sequence alignment (7) 7
biotechnology & applied microbiology (6) 6
carbohydrate sequence (6) 6
cleavage (6) 6
endoplasmic reticulum (6) 6
endoplasmic-reticulum (6) 6
gene (6) 6
gene expression (6) 6
glycoprotein (6) 6
glycoproteins - metabolism (6) 6
gpi, glycosylphosphatidylinositol (6) 6
microbiology (6) 6
mutagenesis, site-directed (6) 6
peptide (6) 6
peptide-n4- asparagine amidase (6) 6
recombinant proteins - chemistry (6) 6
tunicamycin (6) 6
wt, wild-type (6) 6
amidohydrolases - metabolism (5) 5
carbohydrates (5) 5
cattle (5) 5
cho cells (5) 5
disease (5) 5
endoglycosidase (5) 5
escherichia-coli (5) 5
fbs, fetal bovine serum (5) 5
gene expression regulation (5) 5
hek293 cells (5) 5
hela cells (5) 5
igg (5) 5
kinetics (5) 5
lectins (5) 5
life (5) 5
original paper (5) 5
peptide-n4- asparagine amidase - chemistry (5) 5
prion protein (5) 5
protein conformation (5) 5
protein folding (5) 5
proteolysis (5) 5
proteomics (5) 5
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Scientific Reports, ISSN 2045-2322, 12/2019, Volume 9, Issue 1, pp. 4865 - 13
Enzyme immobilization is widely applied in biocatalysis to improve stability and facilitate recovery and reuse of enzymes. However, high cost of supporting... 
3-DIMENSIONAL STRUCTURE | MICROWAVE-ASSISTED DEGLYCOSYLATION | IN-VITRO | DISPLAY | PNGASE-F | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | PROTEIN EXPRESSION | GLYCANS | MALDI-MS | MASS-SPECTROMETRY | Deglycosylation | Enzymes | Capsid protein | Peptides | Centrifugation | Phages | Immobilization | Industrial applications | Nanoparticles | Capsids | Self-assembly | Catalysis | N-glycosidase | Binding sites
Journal Article
Journal of Chromatography A, ISSN 0021-9673, 12/2013, Volume 1322, pp. 54 - 61
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid,... 
PNGase F | Deglycosylation | Enzyme microreactor | Oriented immobilization | Monolith | REACTORS | CHEMISTRY, ANALYTICAL | GLYCANS | TRYPSIN | BIOCHEMICAL RESEARCH METHODS | GLYCOPROTEINS | ESI-MS | CHROMATOGRAPHY | MASS-SPECTROMETRY | POROUS POLYMER MONOLITH | PEPTIDE-N-4-(N-ACETYL-BETA-D-GLUCOSAMINYL)ASPARAGINE AMIDASE
Journal Article
Molecular Biotechnology, ISSN 1073-6085, 8/2015, Volume 57, Issue 8, pp. 735 - 745
Journal Article
Protein Expression and Purification, ISSN 1046-5928, 05/2014, Volume 97, pp. 17 - 22
Journal Article
mAbs, ISSN 1942-0862, 07/2015, Volume 7, Issue 4, pp. 732 - 742
To monitor the Fc glycosylation of therapeutic immunoglobulin G in bioprocess development, product characterization and release analytics, reliable techniques... 
IgG glycosylation | LC-MS | method comparison | HILIC-UPLC | mass spectrometry | ESI-MS | MALDI-MS | monoclonal antibody (mAb) | glycan analysis | Glycan analysis | Monoclonal antibody (mAb) | Method comparison | Mass spectrometry | ANTIBODIES | MEDICINE, RESEARCH & EXPERIMENTAL | 2-aminobenzamide | proteolytic enzyme like protease from Streptococcus pyrogenes | PNGase F | IgG | GLYCOPROTEINS | 2-AB | N-GLYCOSYLATION | capillary electrophoresis | high-performance anion exchange chromatography with pulsed amperometric detection | IdeS protease | GLYCOPEPTIDES | immunoglobulin G | OLIGOSACCHARIDES | ACID | liquid chromatography-mass spectrometry | fragment crystallizable | Fab | MALDI | GLYCANS | HILIC-UHPLC | Peptide-N-Glycosidase F | HPAEC-PAD | PGC-MS | IDENTIFICATION | total ion chromatogram | mAb | TIC | RP-HPLC | hydrophilic interaction liquid chromatography-ultra high performance liquid chromatography | fragment antigen-binding | porous graphitized carbon chromatography- mass spectrometry | electrospray ionization-mass spectrometry | reversed phase high performance liquid chromatography | monoclonal antibody | GLYCOMICS | matrix assisted laser desorption ionization | LCMS | Cricetinae | Animals | Cricetulus | Humans | Immunoglobulin G - chemistry | Recombinant Proteins - chemistry | Sialic Acids - analysis | Immunoglobulin Fc Fragments - chemistry | Glycosylation | Antibodies, Monoclonal - chemistry | CHO Cells
Journal Article
Journal Article
Biochemical Journal, ISSN 0264-6021, 04/2015, Volume 467, Issue 1, pp. 127 - 139
ATP-binding cassette, subfamily B (ABCB) 6 is a homodimeric ATP-binding cassette (ABC) transporter present in the plasma membrane and in the intracellular... 
ATP-binding cassette (ABC) transporter | Trafficking | Adenosine 5′-triphosphatase (ATPase) | Lysosome | ATP-binding cassette | Transmembrane domain | Subfamily B (ABCB) 6 | adenosine 5 '-triphosphatase (ATPase) | B6 ABCB6 | MEMBRANE | ATPASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | CONDUCTANCE REGULATOR | INSECT CELLS | P-GLYCOPROTEIN | BINDING CASSETTE TRANSPORTER | MHC CLASS-I | subfamily B (ABCB) 6 | transmembrane domain | MUTATIONS | MULTIDRUG-RESISTANCE | lysosome | trafficking | Humans | Recombinant Fusion Proteins - metabolism | ATP-Binding Cassette Transporters - chemistry | Endocytosis | Lysosomes - metabolism | ATP-Binding Cassette Transporters - genetics | Adenosine Triphosphate - metabolism | ATP-Binding Cassette Transporters - metabolism | Cell Membrane - metabolism | Dimerization | Peptide Fragments - genetics | Protein Structure, Tertiary | Microscopy, Electron, Transmission | Peptide Fragments - metabolism | Multivesicular Bodies - ultrastructure | Mutant Proteins - metabolism | Cell Membrane - ultrastructure | Recombinant Fusion Proteins - chemistry | Protein Folding | Protein Sorting Signals | Protein Transport | Hydrolysis | Lysosomes - ultrastructure | Peptide Fragments - chemistry | Mutant Proteins - chemistry | K562 Cells | Multivesicular Bodies - metabolism | HeLa Cells | Amino Acid Substitution | Lan, Langereis | P-gp, P-glycoprotein | NP-40, nonyl phenoxypolyethoxylethanol-40 | PAG, Protein A–gold | TfR, transferrin receptor | TMD0, N-terminal transmembrane domain | ABC, ATP-binding cassette | HA, human influenza haemagglutinin | MRP, multidrug resistance-associated protein | NBD, nucleotide-binding domain | HRP, horseradish peroxidase | TMD, transmembrane domain | RT, room temperature | NEM, N-ethylmaleimide | NA, numerical aperture | adenosine 5′-triphosphatase (ATPase) | PNGase-F, peptide N-glycosidase F | MDR, multidrug resistance protein | DPBS, Dulbecco’s modified phosphate-buffered saline | PFA, paraformaldehyde
Journal Article
Biochemical Journal, ISSN 0264-6021, 03/2007, Volume 402, Issue 3, pp. 515 - 523
Wnt-5a is a representative ligand that activates a beta-catenin-independent pathway in Writ signalling. In the present paper, the roles of the... 
Posttranslational modification | Frizzled 5 (Fz5) | Glycosylation | Palmitoylation | Wnt-5a | PROTEIN | PHOSPHORYLATION | frizzled 5 (Fz5) | BIOCHEMISTRY & MOLECULAR BIOLOGY | glycosylation | post-translational modification | BETA-CATENIN | INVASION | PATHWAY | CELL-MIGRATION | GROWTH | FOCAL ADHESION KINASE | DEPENDENT ACTIVATION | EXPRESSION | palmitoylation | Proto-Oncogene Proteins - metabolism | Amino Acid Sequence | Cell Line | Signal Transduction | Humans | Molecular Sequence Data | Proto-Oncogene Proteins - genetics | Proto-Oncogene Proteins - chemistry | Wnt-5a Protein | Wnt Proteins - metabolism | Transcription Factor 7-Like 2 Protein | Wnt Proteins - chemistry | Wnt Proteins - isolation & purification | TCF Transcription Factors - metabolism | Sequence Alignment | Animals | Wnt Proteins - genetics | Fatty Acids, Monounsaturated - metabolism | Protein Binding | Mice | Protein Processing, Post-Translational | Proto-Oncogene Proteins - isolation & purification | Cell Movement | HEK-293T cells, HEK-293 cells expressing the large T-antigen of SV40 (simian virus 40) | CM, conditioned medium | WT, wild-type | ECM, extracellular matrix | NLK, Nemo-like kinase | LRP5 | JNK, c-Jun N-terminal kinase | GFP, green fluorescent protein | Fz, Frizzled | PKC, protein kinase C | FAK, focal adhesion kinase | CRD, cysteine-rich domain | Lef, lymphoid enhancer factor | MALDI–TOF-MS, matrix-assisted laser-desorption ionization–time-of-flight MS | 6, lipoprotein receptor-related protein 5 | siRNA, small interfering RNA | Tcf, T-cell factor | PNGase F, peptide N-glycosidase F | HEK-293, human embryonic kidney-293 | TFA, trifluoroacetic acid
Journal Article
Journal Article
Biochemical Journal, ISSN 0264-6021, 08/2012, Volume 445, Issue 3, pp. 403 - 411
N-linked 21ycosylation is a critical determinant of protein structure and function, regulating processes such as protein folding, stability and localization,... 
Type II transforming growth factor β receptor (TβRII) | N-linked glycosylation | Cell surface transport | Transforming growth factor β sensitivity (TGF-β sensitivity) | Transforming growth factor β signalling (TGF-β signalling) | transforming growth factor beta signalling (TGF-beta signalling) | OLIGOSACCHARIDES | MEMBRANE | STABILITY | BIOCHEMISTRY & MOLECULAR BIOLOGY | PHENOTYPE | MECHANISMS | type II transforming growth factor beta receptor (T beta RII) | transforming growth factor beta sensitivity (TGF-beta sensitivity) | CELL-SURFACE TRANSPORT | BIOSYNTHESIS | TUMOR-SUPPRESSOR | cell surface transport | PROTEINS | EXPRESSION | Receptors, Transforming Growth Factor beta - genetics | Receptors, Transforming Growth Factor beta - chemistry | Humans | Molecular Sequence Data | Glycosylation - drug effects | HEK293 Cells | Conserved Sequence | Cell Membrane - metabolism | Protein-Serine-Threonine Kinases - metabolism | Recombinant Proteins - metabolism | Amino Acid Sequence | Cell Line | Alkaloids - pharmacology | Mutagenesis, Site-Directed | Protein-Serine-Threonine Kinases - genetics | Recombinant Proteins - chemistry | Recombinant Proteins - genetics | Hep G2 Cells | Sequence Homology, Amino Acid | Receptors, Transforming Growth Factor beta - metabolism | Signal Transduction - drug effects | Tunicamycin - pharmacology | Protein-Serine-Threonine Kinases - chemistry | HeLa Cells | Transforming Growth Factor beta - metabolism | Amino Acid Substitution | Asparagine - chemistry | transforming growth factor β sensitivity (TGF-β sensitivity) | KIF, kifunensine | dNG, deficient N-linked glycosylation | TβRII, type II TGF-β receptor | WT, wild-type | Tun, tunicamycin | FBS, fetal bovine serum | TGF-β, transforming growth factor β | Endo H, endoglycosidase H | HEK, human embryonic kidney | DAPI, 4′,6-diamidino-2-phenylindole | type II transforming growth factor β receptor (TβRII) | PNGase F, peptide N-glycosidase F | PEI, polyethyleneimine | GlcNAc, N-acetylglucosamine | transforming growth factor β signalling (TGF-β signalling) | TβRI, type I TGF-β receptor | ER, endoplasmic reticulum | PDI, protein disulfide-isomerase | RT, room temperature
Journal Article
BBA - Molecular Cell Research, ISSN 0167-4889, 12/2014, Volume 1843, Issue 12, pp. 2900 - 2912
Mammalian cellular repressor of E1A-stimulated genes is a lysosomal glycoprotein implicated in cellular growth and differentiation. The genome of the fruit fly... 
Cathepsin | Proteolytic maturation | Protein targeting | Mannose 6-phosphate | CREG | Lysosome | CANCER-CELLS | FACTOR-II RECEPTOR | BIOCHEMISTRY & MOLECULAR BIOLOGY | PROCATHEPSIN-B | GLYCOSYLATION | CELL BIOLOGY | IN-VITRO | ENZYME | SIGNALING PATHWAY | CARDIAC-HYPERTROPHY | GROWTH | Physiological aspects | Genetic research | Fruit-flies | Genes | Drosophila | Cells
Journal Article
Journal Article
Journal of Structural Biology, ISSN 1047-8477, 04/2016, Volume 194, Issue 1, pp. 1 - 7
We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or... 
X-ray crystallography | Mammalian cell expression | Glycoproteins | Molecular replacement | Crystallization | Maltose-binding protein fusion | SYSTEM | STRUCTURE REFINEMENT | DENSITY MODIFICATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | GLYCOSYLATION | CELL BIOLOGY | HIGH-LEVEL EXPRESSION | BIOPHYSICS | PURIFICATION | GLYCOPROTEIN | FUSION PROTEINS | Amino Acid Sequence | Cricetinae | Gene Expression | Cricetulus | Humans | Bacterial Proteins - chemistry | Bacterial Proteins - genetics | Models, Molecular | Crystallography, X-Ray | Maltose-Binding Proteins - metabolism | Recombinant Fusion Proteins - chemistry | Recombinant Fusion Proteins - metabolism | Sf9 Cells | Animals | Base Sequence | HEK293 Cells | Maltose-Binding Proteins - chemistry | Recombinant Fusion Proteins - genetics | Bacterial Proteins - metabolism | Protein Conformation | Mutation | Maltose-Binding Proteins - genetics | CHO Cells | Proteins | Chemical properties | Cells | Protein binding | Tyrosine | Phosphatases | Proteases | Chromatography | Sugars | CHO, Chinese hamster ovary | Crypα, cell adhesion molecule-like tyrosine phosphatase α | HEK, human embryonic kidney | Endo H, Endoglycosidase H | UMOD, uromodulin | AKH, adipokinetic hormone | SEC, size-exclusion chromatography | PEI, polyethylenimine | PDB, Protein Data Bank | VERL, vitelline envelope receptor for lysin | Technical Note | TEVCS, tobacco etch virus protease cleavage site | ORF, open reading frame | TEV, tobacco etch virus | mMBP, mammalianized maltose-binding protein | ENG, endoglin | PNGase F, peptide N-glycosidase F | IMAC, immobilized metal affinity chromatography | CD105 | MBP, maltose-binding protein
Journal Article
Biochemical Journal, ISSN 0264-6021, 05/2007, Volume 403, Issue 3, pp. 583 - 591
Wheat (Triticum aestivum) contains a previously unknown type of xylanase (EC 3.2.1.8) inhibitor, which is described in the present paper for the first time.... 
Slow tight binding | Wheat (Triticum aestivum) | Thaumatin-like protein (TLP) | Thaumatin-like xylanase inhibitor (TLXI) | Xylanase inhibitor | Heterologous expression | ASPARTIC PROTEASE INHIBITOR | XIP-I | BIOCHEMISTRY & MOLECULAR BIOLOGY | heterologous expression | wheat (Triticum aestivum) | thaumatin-like xylanase inhibitor (TLXI) | TAXI-TYPE | thaumatin-like protein (TLP) | TIGHT-BINDING-INHIBITION | STRUCTURAL BASIS | slow tight binding | PLANT DEFENSE | BARLEY | DIFFERENT CEREALS | xylanase inhibitor | ENDOXYLANASE INHIBITORS | PROTEINS | Amino Acid Sequence | Endo-1,4-beta Xylanases - antagonists & inhibitors | Xylans - metabolism | Electrophoresis, Polyacrylamide Gel | Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase - metabolism | Molecular Sequence Data | Glycosylation | Mesylates - chemistry | Enzyme Inhibitors - isolation & purification | Time Factors | Plant Proteins - chemistry | Base Sequence | Enzyme Inhibitors - chemistry | Mass Spectrometry | Cloning, Molecular | Kinetics | Triticum - chemistry | 4-MUX2, 4-methylumbelliferyl-β-D-xylobioside | (r)TLXI, (recombinant) thaumatin-like xylanase inhibitor | TFMSA, trifluoromethanesulfonic acid | GH, glycoside hydrolase family | I | [E]50, ratio of inhibitor concentration to enzyme concentration necessary to obtain 50% inhibition | EST, expressed sequence tag | PNGase F, peptide N-glycosidase F | XIP, xylanase inhibitor protein | TAXI, Triticum aestivum xylanase inhibitor | RACE, rapid amplification of cDNA ends | TLP, thaumatin-like protein | SPR, surface plasmon resonance | CEC, cation-exchange chromatography
Journal Article