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Nature protocols, ISSN 1754-2189, 2006, Volume 1, Issue 6, pp. 3111 - 3120
We describe a rapid method for the production of fusion PCR products that can be used, generally without band purification, to transform Aspergillus nidulans.... 
Gene Targeting - methods | Gene Transfer Techniques | Aspergillus nidulans - genetics | Protoplasts | Polymerase Chain Reaction - methods
Journal Article
Genome Biology, ISSN 1474-7596, 05/2018, Volume 19, Issue 1, pp. 59 - 9
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved... 
SgRNA forms | Herbicide resistance | Wheat | Cas9-adenosine deaminase | Rice | sgRNA forms | CRISPR | RESISTANT | ENDONUCLEASE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | GENOMIC DNA | Flow cytometry | Adenosine | tRNA | Base pairs | Thymine | Protoplasts | Adenine | Genomes | Adenosine deaminase | Conversion | Efficiency | Point mutation | Cytosine | Mutation | Deoxyribonucleic acid--DNA
Journal Article
PLoS ONE, ISSN 1932-6203, 04/2015, Volume 10, Issue 4, p. e0121875
Epichloe species (including the former genus Neotyphodium) are fungal symbionts of many agronomically important forage grasses, and provide their grass hosts... 
CONIDIAL ANASTOMOSIS TUBES | ASPERGILLUS-NIDULANS | MULTIDISCIPLINARY SCIENCES | FUSARIUM-OXYSPORUM | TALL FESCUE | NEOTYPHODIUM ENDOPHYTES | VERTICAL TRANSMISSION | HORIZONTAL GENE-TRANSFER | FILAMENTOUS FUNGI | FUNGAL ENDOPHYTES | INTERSPECIFIC HYBRIDIZATION | Hyphae - genetics | Symbiosis - physiology | Epichloe - classification | Epichloe - ultrastructure | Green Fluorescent Proteins - genetics | Phylogeny | Nuclear Fusion | Protoplasts - metabolism | Cell Nucleus - metabolism | Hybridization, Genetic | Protoplasts - ultrastructure | Endophytes | Spores, Fungal - genetics | Genes, Reporter | Gene Expression Regulation, Fungal | Green Fluorescent Proteins - metabolism | Poaceae - microbiology | Hyphae - ultrastructure | Bacterial Proteins - genetics | Poaceae - physiology | Epichloe - genetics | Fungal Proteins - genetics | Cell Nucleus - ultrastructure | Plasmids - metabolism | Cell Nucleus - genetics | Hyphae - metabolism | Epichloe - metabolism | Spores, Fungal - metabolism | Spores, Fungal - ultrastructure | Plasmids - chemistry | Bacterial Proteins - metabolism | Luminescent Proteins - genetics | Fungal Proteins - metabolism | Luminescent Proteins - metabolism | Protoplast fusion | Hybrids | Recombination | Genes | Plant biology | Interspecific | Genomes | Hybridization | Grasses | Nuclei | Fungi | Symbionts | Nuclear fusion | Plasmids | Agronomy | Hyphae | Phylogenetics | Evolution | Genetic engineering | Herbivores | Species | Chromosomes | Cytoplasm
Journal Article
BioMed Research International, ISSN 2314-6133, 01/2020, Volume 2020, pp. 1 - 10
Saccharomyces cerevisiae is a typical fermentation yeast in beer production. Improving ethanol tolerance of S. cerevisiae will increase fermentation... 
Protoplast fusion | Yeasts | Enzymes | Yeast | Ethanol | Cloning | Genomes | Capital | Glucose | Fermentation | Sulfur dioxide | Beer | Capital costs | Drug tolerance | Genetic engineering | Evolution & development | Potassium
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2018, Volume 8, Issue 1, pp. 2922 - 10
Aspergillus oryzae is an industrially important filamentous fungus used for Japanese traditional food fermentation and heterologous protein production.... 
TRANSFORMATION | CONIDIAL ANASTOMOSIS TUBES | EPICHLOE-FESTUCAE | HYPHAL FUSION | GENE | RECOGNITION | MULTIDISCIPLINARY SCIENCES | BIOLOGY | SCLEROTIA | NEUROSPORA-CRASSA | DELETION | Protoplast fusion | Morphology | Anastomosis | Fermented food | Cytology | Cell fusion | Fusion protein | Fermentation | Growth stage
Journal Article
FEMS Microbiology Letters, ISSN 0378-1097, 04/2019, Volume 366, Issue 8
In our previous study, we showed that cell fusion occurred in spheroplasts of Deinococcus grandis at 200 mM calcium chloride in the incubation medium.... 
Extra-huge cell | Spheroplast enlargement | Calcium ion | Deinococcus grandis | Outer membrane fusion | outer membrane fusion | LIPOPOLYSACCHARIDE | BACTERIUM | calcium ion | PROTOPLASTS | MICROBIOLOGY | ERYTHROBACTER-LITORALIS | extra-huge cell | spheroplast enlargement
Journal Article
Applied Physics Letters, ISSN 0003-6951, 07/2013, Volume 103, Issue 3, p. 33701
We report a study on the laser-induced fusion of human embryonic stem cells (hESCs) at the single-cell level. Cells were manipulated by optical tweezers and... 
SOMATIC-CELLS | PLANT-PROTOPLASTS | PHYSICS, APPLIED | FREQUENCY | LASER RADIATION | IRRADIATION | BIOPHYSICS | FLUORESCENCE | CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS | BIOLOGICAL EFFECTS | CELL DIFFERENTIATION | STEM CELLS | PROTEINS | RADIATION PRESSURE | EFFICIENCY | PULSES
Journal Article
Journal Article
Plant Cell, Tissue and Organ Culture (PCTOC), ISSN 0167-6857, 10/2017, Volume 131, Issue 1, pp. 1 - 14
Somatic hybridization by protoplast fusion is used in breeding programs to obtain plant material that has inherited valuable traits from two different species,... 
Life Sciences | Plant Pathology | Nuclear DNA content | Plant Genetics and Genomics | AFLP | Plant Physiology | Gentian | ISSR | Somatic hybridization | Plant Sciences | CAPS | MOLECULAR CHARACTERIZATION | RFLP ANALYSIS | PONCIRUS-TRIFOLIATA