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Journal of Biological Chemistry, ISSN 0021-9258, 03/2006, Volume 281, Issue 11, pp. 6904 - 6909
Journal Article
Journal Article
Journal Article
Journal Article
Analytical Biochemistry, ISSN 0003-2697, 12/2015, Volume 490, pp. 66 - 72
Hedgehog signaling is critical for correct embryogenesis and tissue development. However, on maturation, signaling is also found to be aberrantly activated in... 
MBOAT | Click chemistry | Hedgehog acyltransferase | Protein palmitoylation | CHEMISTRY, ANALYTICAL | HUMAN-CELLS | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | PROBES | PROTEIN LIPIDATION | PATHWAY | INHIBITORS | CANCER PROGRESSION | SONIC-HEDGEHOG | Acyltransferases - antagonists & inhibitors | Hedgehog Proteins - chemistry | Detergents - chemistry | Humans | Hedgehog Proteins - metabolism | Substrate Specificity | Acyltransferases - metabolism | Acyltransferases - genetics | Recombinant Fusion Proteins - metabolism | Maltose - chemistry | Protein Processing, Post-Translational - drug effects | HEK293 Cells | Palmitoyl Coenzyme A - analogs & derivatives | Oligopeptides - chemistry | Streptavidin - chemistry | Peptide Fragments - metabolism | Biotinylation | Immobilized Proteins - chemistry | Enzyme-Linked Immunosorbent Assay | Click Chemistry | Enzyme Inhibitors - pharmacology | Lipoylation - drug effects | Solubility | Palmitoyl Coenzyme A - metabolism | Fatty Acids, Unsaturated - pharmacology | Oligopeptides - metabolism | Recombinant Fusion Proteins - chemistry | Peptide Fragments - chemistry | Maltose - analogs & derivatives | Streptavidin - metabolism | Immobilized Proteins - metabolism | Acyltransferases - chemistry | Measurement | Post-translational modification | Embryonic development | Detergents, Synthetic | Thiols | Peptides | Analysis | Fatty acids | Enzyme-linked immunosorbent assay | Acylation | OTG, n-octyl β-d-glucopyranoside | PTM, posttranslational modification | SDS, sodium dodecyl sulfate | GOAT, ghrelin-O-acyltransferase | YnC15, heptadec-16-ynoic acid | click–ELISA, click chemistry armed enzyme-linked immunosorbent assay | TBTA, tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine | MBOAT, membrane bound O-acyltransferase | HRP, horseradish peroxidase | PBS, phosphate-buffered saline | Hh, hedgehog | PAGE, polyacrylamide gel electrophoresis | HEK293a, human embryonic kidney 293a | RT, room temperature | BSA, bovine serum albumin | TCEP, tris(2-carboxyethyl)phosphine | CoA, coenzyme A | Shh, sonic hedgehog | CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | HTS, high-throughput screen | cat–ELCCA, catalytic assay using an enzyme-linked click chemistry assay | DDM, n-dodecyl β-d-maltopyranoside | Hhat, hedgehog acyltransferase | PCR, polymerase chain reaction
Journal Article