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1. Full Text Validation Study of a Quantitative Multigene Reverse Transcriptase–Polymerase Chain Reaction Assay for Assessment of Recurrence Risk in Patients With Stage II Colon Cancer
by Richard G. Gray and Philip Quirke and Kelly Handley and Margarita Lopatin and Laura Magill and Frederick L. Baehner and Claire Beaumont and Kim M. Clark-Langone and Carl N. Yoshizawa and Mark Lee and Drew Watson and Steven Shak and David J. Kerr
Journal of Clinical Oncology, ISSN 0732-183X, 12/2011, Volume 29, Issue 35, pp. 4611 - 4619
Purpose We developed quantitative gene expression assays to assess recurrence risk and benefits from chemotherapy in patients with stage II colon cancer.... 
ADJUVANT CHEMOTHERAPY | BREAST-CANCER | TUMOR GENE-EXPRESSION | ONCOLOGY | DEFECTIVE MISMATCH REPAIR | COLORECTAL-CANCER | RAS MUTATIONS | DUKES-B | PREDICT RECURRENCE | MICROSATELLITE-INSTABILITY | FLUOROURACIL | Colonic Neoplasms - genetics | Gene Expression | Genetic Predisposition to Disease | Prospective Studies | Colonic Neoplasms - drug therapy | Humans | Middle Aged | Male | Neoplasm Recurrence, Local - pathology | Disease-Free Survival | Reverse Transcriptase Polymerase Chain Reaction - standards | Colonic Neoplasms - pathology | Reverse Transcriptase Polymerase Chain Reaction - methods | Aged, 80 and over | Adult | Female | Neoplasm Recurrence, Local - genetics | Aged | Neoplasm Staging | Index Medicus
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2. Full Text The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
by Bustin, Stephen A and Benes, Vladimir and Garson, Jeremy A and Hellemans, Jan and Huggett, Jim and Kubista, Mikael and Mueller, Reinhold and Nolan, Tania and Pfaffl, Michael W and Shipley, Gregory L and Vandesompele, Jo and Wittwer, Carl T
Clinical Chemistry, ISSN 0009-9147, 04/2009, Volume 55, Issue 4, pp. 611 - 622
BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is... 
POLYMERASE-CHAIN-REACTION | MULTIPLEX PCR | RELATIVE QUANTIFICATION | MESSENGER-RNA | INTERNAL CONTROL | EXTERNAL QUALITY-CONTROL | RT-PCR | GENE-EXPRESSION | DNA AMPLIFICATION | MEDICAL LABORATORY TECHNOLOGY | PROBE DATABASE | Molecular Diagnostic Techniques | Nucleic Acids - chemistry | Time Factors | Humans | Polymerase Chain Reaction - standards | Publishing - standards | Terminology as Topic | Nucleic Acids - genetics | Reverse Transcription - genetics | Polymerase Chain Reaction - methods | Polymerase chain reaction | Usage | Methodology | Methods | Biological variation | Publications | Genetically altered foods | Risk assessment | Nonfiction | Life sciences | Experiments | Product differentiation | Index Medicus
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3. Full Text A diagnostic polymerase chain reaction assay for Zika virus
by Balm, Michelle N.D and Lee, Chun Kiat and Lee, Hong Kai and Chiu, Lily and Koay, Evelyn S.C and Tang, Julian W
Journal of Medical Virology, ISSN 0146-6615, 09/2012, Volume 84, Issue 9, pp. 1501 - 1505
Zika virus (ZIKV) is a mosquito‐borne flavivirus. Infection results in a dengue‐like illness with fever, headache, malaise, and a maculopapular rash. Nearly... 
arthralgia | Chikungunya | surveillance | febrile illness | dengue | Febrile illness | Surveillance | Arthralgia | Dengue | INFECTIONS | STATE | MICRONESIA | VIROLOGY | ASIA | DISEASE | PCR | FEVER | Genome, Viral | Limit of Detection | Singapore | Zika Virus - genetics | Humans | Fever - diagnosis | Male | Fever - virology | Zika Virus Infection - virology | Reference Standards | RNA, Viral - genetics | Reverse Transcriptase Polymerase Chain Reaction - standards | Sequence Analysis, RNA | Zika Virus Infection - diagnosis | Adult | Female | Molecular Diagnostic Techniques - standards | RNA, Viral - isolation & purification | Polymerase chain reaction | Virus diseases | Medical colleges | Mosquitoes | Circuit design | RNA | Health aspects | Public health | Immunoassay | Virology | Index Medicus
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4. Full Text MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
by Bustin, S.A and Beaulieu, J.F and Huggett, J and Jaggi, R and Kibenge, F.S and Olsvik, P.A and Penning, L.C.|info:eu-repo/dai/nl/110369181 and Toegel, S
BMC Molecular Biology [E], ISSN 1471-2199, 2010, Volume 11, Issue 1, pp. 74 - 74
MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments Stephen A Bustin1 ,... 
BIOCHEMISTRY & MOLECULAR BIOLOGY | Fluorescence | Polymerase Chain Reaction - standards | Publishing - standards | Peer Review, Research - standards | Polymerase Chain Reaction - instrumentation | Periodicals as Topic - standards | Polymerase Chain Reaction - methods | Polymerase chain reaction | Molecular biology | Analysis | Medical research | Health sciences | Publications | Colleges & universities | Green chemistry | Veterinary medicine | Experiments | Methods | Deoxyribonucleic acid--DNA | Index Medicus
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5. Full Text The digital MIQE guidelines: Minimum information for publication of quantitative digital PCR experiments
by Huggett, Jim F and Foy, Carole A and Benes, Vladimir and Emslie, Kerry and Garson, Jeremy A and Haynes, Ross and Hellemans, Jan and Kubista, Mikael and Mueller, Reinhold D and Nolan, Tania and Pfaffl, Michael W and Shipley, Gregory L and Vandesompele, Jo and Wittwer, Carl T and Bustin, Stephen A
Clinical Chemistry, ISSN 0009-9147, 06/2013, Volume 59, Issue 6, pp. 892 - 902
There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the... 
REAL-TIME PCR | PLATFORM | SYSTEM | DESIGN | POLYMERASE-CHAIN-REACTION | ABSOLUTE QUANTITATION | RT-PCR | DNA | QUANTIFICATION | MEDICAL LABORATORY TECHNOLOGY | COPY NUMBER VARIATION | Computers - standards | Computers - statistics & numerical data | Real-Time Polymerase Chain Reaction - standards | Guidelines as Topic | Polymerase chain reaction | Medicine, Experimental | Medical research | Research | Studies | Automation | Mutation | Reporting requirements | Experiments | Methods | Index Medicus
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6. Quantification of mRNA using real-time RT-PCR
by Nolan, Tania and Hands, Rebecca E and Bustin, Stephen A
Nature Protocols, ISSN 1754-2189, 08/2006, Volume 1, Issue 3, pp. 1559 - 1582
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine,... 
POLYMERASE-CHAIN-REACTION | REVERSE-TRANSCRIPTASE | BIOCHEMICAL RESEARCH METHODS | QUANTITATIVE PCR | DNA AMPLIFICATION | SENSITIVITY | SAMPLES | NORMALIZATION | INHIBITORS | HOUSEKEEPING GENES | EXPRESSION | RNA, Messenger - analysis | Gene Expression Profiling - methods | Data Interpretation, Statistical | Reverse Transcriptase Polymerase Chain Reaction - standards | Reverse Transcriptase Polymerase Chain Reaction - methods
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7. Full Text Dried Blood Spot Real-time Polymerase Chain Reaction Assays to Screen Newborns for Congenital Cytomegalovirus Infection
by Boppana, Suresh B and Ross, Shannon A and Novak, Zdenek and Shimamura, Masako and Tolan, Robert W and Palmer, April L and Ahmed, Amina and Michaels, Marian G and Sánchez, Pablo J and Bernstein, David I and Britt, William J and Fowler, Karen B and National Institute on Deafness and Other Communication Disorders CMV and Hearing Multicenter Screening (CHIMES) Study, for the and Natl Inst Deafness Other Commun and CMV Hearing Multictr Screening and National Institute on Deafness and Other Communication Disorders CMV and Hearing Multicenter Screening (CHIMES) Study
JAMA, ISSN 0098-7484, 04/2010, Volume 303, Issue 14, pp. 1375 - 1382
CONTEXT Reliable methods to screen newborns for congenital cytomegalovirus (CMV) infection are needed for identification of infants at increased risk of... 
DNA DETECTION | MEDICINE, GENERAL & INTERNAL | VIRUS | SENSORINEURAL HEARING-LOSS | DISEASE | VIRAL-LOAD | CMV INFECTION | FILTER-PAPER | PREVALENCE | RAPID DETECTION | CHILDREN | Cytomegalovirus - genetics | Cytomegalovirus - isolation & purification | Reproducibility of Results | Humans | DNA, Viral - analysis | Cytomegalovirus Infections - diagnosis | Neonatal Screening | Polymerase Chain Reaction - methods | Saliva - virology | Sensitivity and Specificity | Polymerase Chain Reaction - standards | Cytomegalovirus Infections - congenital | Infant, Newborn | Polymerase chain reaction | Infants (Newborn) | Evaluation | Usage | Care and treatment | Cytomegalovirus infections | Diagnosis | Medical screening | Congenital diseases | Newborn babies | Biological assays | Herpes viruses | Index Medicus | Abridged Index Medicus
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8. Full Text Absolute quantification by droplet digital PCR versus analog real-time PCR
by Hindson, Christopher M and Chevillet, John R and Briggs, Hilary A and Gallichotte, Emily N and Ruf, Ingrid K and Hindson, Benjamin J and Vessella, Robert L and Tewari, Muneesh
Nature Methods, ISSN 1548-7091, 10/2013, Volume 10, Issue 10, pp. 1003 - 1005
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of... 
DNA COPY NUMBER | RNA | QUANTITATION | BIOCHEMICAL RESEARCH METHODS | SERUM | MICRORNAS | CANCER | Limit of Detection | Reproducibility of Results | Biomarkers, Tumor - analysis | Humans | Real-Time Polymerase Chain Reaction - methods | Male | Real-Time Polymerase Chain Reaction - standards | Prostatic Neoplasms - diagnosis | Biomarkers, Tumor - blood | MicroRNAs - analysis | Sensitivity and Specificity | Prostatic Neoplasms - blood | MicroRNAs - blood | Polymerase chain reaction | Comparative analysis | Methods | Quantitative genetics | Biomarkers | Gene expression | MicroRNAs | Index Medicus
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9. Full Text A novel and universal method for microRNA RT-qPCR data normalization
by Mestdagh, Pieter and Van Vlierberghe, Pieter and De Weer, An and Muth, Daniel and Westermann, Frank and Speleman, Frank and Vandesompele, Jo
Genome Biology, ISSN 1474-7596, 06/2009, Volume 10, Issue 6, pp. R64 - R64
Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all... 
REFERENCE RNA | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | QUANTIFICATION | TRANSCRIPTION | GENETICS & HEREDITY | GENE-EXPRESSION | PATTERNS | NEUROBLASTOMA | TIME QUANTITATIVE PCR | TISSUES | DIFFERENTIATION | PROBES | Oncogene Proteins - genetics | Humans | Neuroblastoma - genetics | Gene Expression Regulation, Neoplastic | Oncogene Proteins - metabolism | Nuclear Proteins - metabolism | False Positive Reactions | Down-Regulation - genetics | Algorithms | Reverse Transcriptase Polymerase Chain Reaction - standards | Reverse Transcriptase Polymerase Chain Reaction - methods | Statistics, Nonparametric | Cell Line, Tumor | MicroRNAs - genetics | N-Myc Proto-Oncogene Protein | Nuclear Proteins - genetics | Index Medicus | Method
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10. Full Text Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
by Ramakers, Christian and Ruijter, Jan M and Deprez, Ronald H.Lekanne and Moorman, Antoon F.M
Neuroscience Letters, ISSN 0304-3940, 2003, Volume 339, Issue 1, pp. 62 - 66
Quantification of mRNAs using real-time polymerase chain reaction (PCR) by monitoring the product formation with the fluorescent dye SYBR Green I is being... 
Quantitative polymerase chain reaction (PCR) | SYBR ® green I | Real-time PCR | mRNA quantification | Linear regression | PCR efficiency | Standard curve method | Comparative Ct method | method | SYBR® green I | Comparative C | quantitative polymerase chain reaction (PCR) | SYBR (R) green I | linear regression | comparative C-t method | real-time PCR | standard curve method | NEUROSCIENCES | DNA, Complementary - analysis | Time Factors | Organic Chemicals | Polymerase Chain Reaction - standards | RNA, Messenger - analysis | Linear Models | Calibration | Polymerase Chain Reaction - statistics & numerical data | Fluorescent Dyes | Index Medicus
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11. Full Text Telomere length measurement by a novel monochrome multiplex quantitative PCR method
by Cawthon, Richard M
Nucleic Acids Research, ISSN 0305-1048, 2/2009, Volume 37, Issue 3, pp. e21 - e21
The current quantitative polymerase chain reaction (QPCR) assay of telomere length measures telomere (T) signals in experimental DNA samples in one set of... 
POLYMERASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | Telomere - chemistry | Reference Standards | Reproducibility of Results | Temperature | Humans | Polymerase Chain Reaction - standards | Gene Dosage | Tandem Repeat Sequences | DNA Primers | Albumins - genetics