PROTEOMICS, ISSN 1615-9853, 02/2002, Volume 2, Issue 2, pp. 151 - 156
A novel free‐flow protein purification technique based on isoelectric electrophoresis is presented, where the proteins are purified in solution without the...
Prefractionation | Isoelectric focusing | Isoelectric purification
Prefractionation | Isoelectric focusing | Isoelectric purification
Journal Article
Journal of Cell Biology, ISSN 0021-9525, 07/2011, Volume 194, Issue 2, pp. 165 - 175
Centrioles/basal bodies (CBBs) are microtubule-based cylindrical organelles that nucleate the formation of centrosomes, cilia, and flagella. CBBs, cilia, and...
CENTROSOME BIOGENESIS | EPITHELIAL-CELLS | LENGTH CONTROL | ACCESSORY STRUCTURES | LC-MS/MS IDENTIFICATION | IPG STRIP PREFRACTIONATION | BASAL BODY | PROTEOMIC ANALYSIS | ANCESTRAL EUKARYOTE | INTRAFLAGELLAR TRANSPORT | CELL BIOLOGY | Physiological aspects | Centrioles | Cilia and ciliary motion | Flagella (Microbiology) | Reviews
CENTROSOME BIOGENESIS | EPITHELIAL-CELLS | LENGTH CONTROL | ACCESSORY STRUCTURES | LC-MS/MS IDENTIFICATION | IPG STRIP PREFRACTIONATION | BASAL BODY | PROTEOMIC ANALYSIS | ANCESTRAL EUKARYOTE | INTRAFLAGELLAR TRANSPORT | CELL BIOLOGY | Physiological aspects | Centrioles | Cilia and ciliary motion | Flagella (Microbiology) | Reviews
Journal Article
PROTEOMICS, ISSN 1615-9853, 03/2009, Volume 9, Issue 6, pp. 1420 - 1427
The plasma proteome has proven to be one of the most challenging proteomes to profile using currently available proteomics technologies. A plethora of...
Plasma | Prefractionation | Affinity prefractionation | OXIDATIVE STRESS | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | SIALIC-ACID | HUMAN SERUM | ION-TRAP | BREAST-CANCER | HYDRAZIDE CHEMISTRY | IMMUNOAFFINITY FRACTIONATION | BIOMARKER DISCOVERY | LOW-ABUNDANCE PROTEINS | DESORPTION/IONIZATION MASS-SPECTROMETRY | Blood Proteins - analysis | Peptides - blood | Peptides - isolation & purification | Animals | Peptides - chemistry | Humans | Mass Spectrometry | Proteomics - methods | Chemical Fractionation - methods | Chromatography, Affinity - methods
Plasma | Prefractionation | Affinity prefractionation | OXIDATIVE STRESS | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | SIALIC-ACID | HUMAN SERUM | ION-TRAP | BREAST-CANCER | HYDRAZIDE CHEMISTRY | IMMUNOAFFINITY FRACTIONATION | BIOMARKER DISCOVERY | LOW-ABUNDANCE PROTEINS | DESORPTION/IONIZATION MASS-SPECTROMETRY | Blood Proteins - analysis | Peptides - blood | Peptides - isolation & purification | Animals | Peptides - chemistry | Humans | Mass Spectrometry | Proteomics - methods | Chemical Fractionation - methods | Chromatography, Affinity - methods
Journal Article
International Journal of Pharmacy and Pharmaceutical Sciences, 2009, Issue 1, pp. 1 - 23
Journal Article
Petroleum Processing and Petrochemicals, ISSN 1005-2399, 11/2015, Volume 46, Issue 11, pp. 52 - 55
Journal Article
PROTEOMICS, ISSN 1615-9853, 08/2003, Volume 3, Issue 8, pp. 1397 - 1407
The present review deals with a number of prefractionation protocols in preparation for two‐dimensional map analysis, both in the fields of chromatography and...
Prefractionation techniques | Proteome analyses | Review | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | SOLUTION ISOELECTROFOCUSING PRIOR | HAEMOPHILUS-INFLUENZAE | 2-DIMENSIONAL GEL-ELECTROPHORESIS | CARRIER AMPHOLYTES | MASS-SPECTROMETRY | proteome analyses | PREPARATIVE PURIFICATION | prefractionation techniques | FREE-FLOW ELECTROPHORESIS | SAMPLE PREFRACTIONATION | HEPARIN CHROMATOGRAPHY | review | IMMOBILIZED PH GRADIENTS | Electrophoresis, Gel, Two-Dimensional | Proteome | Chromatography, Liquid - methods | Chemical Fractionation
Prefractionation techniques | Proteome analyses | Review | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | SOLUTION ISOELECTROFOCUSING PRIOR | HAEMOPHILUS-INFLUENZAE | 2-DIMENSIONAL GEL-ELECTROPHORESIS | CARRIER AMPHOLYTES | MASS-SPECTROMETRY | proteome analyses | PREPARATIVE PURIFICATION | prefractionation techniques | FREE-FLOW ELECTROPHORESIS | SAMPLE PREFRACTIONATION | HEPARIN CHROMATOGRAPHY | review | IMMOBILIZED PH GRADIENTS | Electrophoresis, Gel, Two-Dimensional | Proteome | Chromatography, Liquid - methods | Chemical Fractionation
Journal Article
ELECTROPHORESIS, ISSN 0173-0835, 06/2013, Volume 34, Issue 11, pp. 1519 - 1525
The article presents a new concept of preparative solution IEF where time requirements and efficiency are similar to gel‐based IEF whereas simple fraction...
Micropreparative | Caseinomacropeptide | Whey | Solution isoelectric focusing | Bioactive peptides | CHEMISTRY, ANALYTICAL | ACIDIC PH | BIOCHEMICAL RESEARCH METHODS | 2-DIMENSIONAL ELECTROPHORESIS | PREPARATIVE ANALYSIS | AMPHOLYTIC DYES | MASS-SPECTROMETRY | FREE-FLOW ELECTROPHORESIS | SAMPLE PREFRACTIONATION | PROTEOME ANALYSIS | PREFRACTIONATION TECHNIQUES | MULTICOMPARTMENT ELECTROLYZER | Isoelectric Focusing - instrumentation | Milk Proteins - isolation & purification | Isoelectric Focusing - methods | Peptides - isolation & purification | Whey Proteins | Equipment Design | Proteins | Strip | Separation | Peptides | Focusing | Raw | Channels
Micropreparative | Caseinomacropeptide | Whey | Solution isoelectric focusing | Bioactive peptides | CHEMISTRY, ANALYTICAL | ACIDIC PH | BIOCHEMICAL RESEARCH METHODS | 2-DIMENSIONAL ELECTROPHORESIS | PREPARATIVE ANALYSIS | AMPHOLYTIC DYES | MASS-SPECTROMETRY | FREE-FLOW ELECTROPHORESIS | SAMPLE PREFRACTIONATION | PROTEOME ANALYSIS | PREFRACTIONATION TECHNIQUES | MULTICOMPARTMENT ELECTROLYZER | Isoelectric Focusing - instrumentation | Milk Proteins - isolation & purification | Isoelectric Focusing - methods | Peptides - isolation & purification | Whey Proteins | Equipment Design | Proteins | Strip | Separation | Peptides | Focusing | Raw | Channels
Journal Article
Journal of Proteomics, ISSN 1874-3919, 02/2013, Volume 79, pp. 114 - 122
The male gamete (sperm) can fertilize an egg, and pass the male genetic information to the offspring. It has long been thought that sperm had a simple protein...
Drug | Human | Proteome | Signaling pathway | Sperm | Mass spectrometry | RNA | DROSOPHILA-MELANOGASTER | BIOCHEMICAL RESEARCH METHODS | ALPHA | IPG STRIP PREFRACTIONATION | IDENTIFICATION | ORIGIN | CAPACITATION | EXPRESSION | ACROSOME REACTION | MOTILITY | Bone morphogenetic proteins | Spermatozoa | Analysis
Drug | Human | Proteome | Signaling pathway | Sperm | Mass spectrometry | RNA | DROSOPHILA-MELANOGASTER | BIOCHEMICAL RESEARCH METHODS | ALPHA | IPG STRIP PREFRACTIONATION | IDENTIFICATION | ORIGIN | CAPACITATION | EXPRESSION | ACROSOME REACTION | MOTILITY | Bone morphogenetic proteins | Spermatozoa | Analysis
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 01/2014, Volume 13, Issue 1, pp. 60 - 75
The kidney, urine, and plasma proteomes are intimately related: proteins and metabolic waste products are filtered from the plasma by the kidney and excreted...
kidney | LC-MS/MS | database | urine | PeptideAtlas | Human Proteome Project | plasma | proteome comparison | TANDEM | PREFRACTIONATION | BIOCHEMICAL RESEARCH METHODS | IDENTIFICATION | COMBINATION | ANNOTATION | EPIDERMAL-GROWTH-FACTOR | HIGH-CONFIDENCE | PROTEINS | EXPRESSION | Chromatography, Liquid | Tandem Mass Spectrometry | Proteome | Proteins - metabolism | Humans | LC-MS
kidney | LC-MS/MS | database | urine | PeptideAtlas | Human Proteome Project | plasma | proteome comparison | TANDEM | PREFRACTIONATION | BIOCHEMICAL RESEARCH METHODS | IDENTIFICATION | COMBINATION | ANNOTATION | EPIDERMAL-GROWTH-FACTOR | HIGH-CONFIDENCE | PROTEINS | EXPRESSION | Chromatography, Liquid | Tandem Mass Spectrometry | Proteome | Proteins - metabolism | Humans | LC-MS
Journal Article
Reproduction, ISSN 1470-1626, 02/2014, Volume 147, Issue 2, pp. R27 - R42
Testicular spermatozoa acquire fertility only after 1 or 2 weeks of transit through the epididymis. At the end of this several meters long epididymal tubule,...
CARBONIC-ANHYDRASE-XIV | REPRODUCTIVE BIOLOGY | PROTEIN PHOSPHATASE 1 | ZONA-PELLUCIDA-BINDING | SOLUBLE ADENYLYL-CYCLASE | TYROSINE PHOSPHORYLATION | MOUSE SPERM | LC-MS/MS IDENTIFICATION | IPG STRIP PREFRACTIONATION | DEVELOPMENTAL BIOLOGY | PROTEOMIC ANALYSIS | MAMMALIAN SPERMATOZOA | Proteins - physiology | Spermatozoa - chemistry | Phosphorylation | Humans | Male | Proteins - analysis | Epididymis - physiology | Sperm Transport - physiology | Sperm Capacitation - physiology | Epididymis - chemistry | Sperm-Ovum Interactions | Animals | Calcium - physiology | Sperm Motility - physiology | Fertility | Proteomics | Bicarbonates - metabolism | Spermatozoa - physiology | Sperm Maturation - physiology | Life Sciences | Reproductive Biology | Other
CARBONIC-ANHYDRASE-XIV | REPRODUCTIVE BIOLOGY | PROTEIN PHOSPHATASE 1 | ZONA-PELLUCIDA-BINDING | SOLUBLE ADENYLYL-CYCLASE | TYROSINE PHOSPHORYLATION | MOUSE SPERM | LC-MS/MS IDENTIFICATION | IPG STRIP PREFRACTIONATION | DEVELOPMENTAL BIOLOGY | PROTEOMIC ANALYSIS | MAMMALIAN SPERMATOZOA | Proteins - physiology | Spermatozoa - chemistry | Phosphorylation | Humans | Male | Proteins - analysis | Epididymis - physiology | Sperm Transport - physiology | Sperm Capacitation - physiology | Epididymis - chemistry | Sperm-Ovum Interactions | Animals | Calcium - physiology | Sperm Motility - physiology | Fertility | Proteomics | Bicarbonates - metabolism | Spermatozoa - physiology | Sperm Maturation - physiology | Life Sciences | Reproductive Biology | Other
Journal Article
Theriogenology, ISSN 0093-691X, 01/2020, Volume 141, pp. 68 - 81
The binding of seminal plasma (SP) proteins by spermatozoa plays an important role in the regulation of sperm epididymal maturation, motility gaining in female...
Proteins | Spermatozoa | Seminal plasma | Proteomics | Cat | PROLACTIN-INDUCIBLE PROTEIN | HUMAN SERUM-ALBUMIN | GLUTATHIONE-PEROXIDASE | SPERM PROTEOME | SEMINAL PLASMA-PROTEINS | IPG STRIP PREFRACTIONATION | MAIN PROTEIN | FERTILITY | REPRODUCTIVE BIOLOGY | VETERINARY SCIENCES | RAT SPERM | BINDING
Proteins | Spermatozoa | Seminal plasma | Proteomics | Cat | PROLACTIN-INDUCIBLE PROTEIN | HUMAN SERUM-ALBUMIN | GLUTATHIONE-PEROXIDASE | SPERM PROTEOME | SEMINAL PLASMA-PROTEINS | IPG STRIP PREFRACTIONATION | MAIN PROTEIN | FERTILITY | REPRODUCTIVE BIOLOGY | VETERINARY SCIENCES | RAT SPERM | BINDING
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2017, Volume 7, Issue 1, pp. 3024 - 13
Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic...
ACUTE KIDNEY INJURY | GLOMERULAR-BASEMENT-MEMBRANE | DIFFERENTIAL EXPRESSION | BIOMARKER DISCOVERY | MULTIDISCIPLINARY SCIENCES | NON-PREFRACTIONATION | DIABETIC-NEPHROPATHY | TANDEM MASS-SPECTROMETRY | ACUTE RENAL INJURY | LARGE GENE LISTS | FOCAL SEGMENTAL GLOMERULOSCLEROSIS | Tandem Mass Spectrometry | Computational Biology - methods | Biomarkers - urine | Databases, Protein | Humans | Molecular Sequence Annotation | Chromatography, Liquid | Proteome | Chromatography, High Pressure Liquid | Proteomics - methods | Urine | Proteins | Mass spectroscopy | Liquid chromatography | Isoelectric focusing
ACUTE KIDNEY INJURY | GLOMERULAR-BASEMENT-MEMBRANE | DIFFERENTIAL EXPRESSION | BIOMARKER DISCOVERY | MULTIDISCIPLINARY SCIENCES | NON-PREFRACTIONATION | DIABETIC-NEPHROPATHY | TANDEM MASS-SPECTROMETRY | ACUTE RENAL INJURY | LARGE GENE LISTS | FOCAL SEGMENTAL GLOMERULOSCLEROSIS | Tandem Mass Spectrometry | Computational Biology - methods | Biomarkers - urine | Databases, Protein | Humans | Molecular Sequence Annotation | Chromatography, Liquid | Proteome | Chromatography, High Pressure Liquid | Proteomics - methods | Urine | Proteins | Mass spectroscopy | Liquid chromatography | Isoelectric focusing
Journal Article
PROTEOMICS, ISSN 1615-9853, 07/2011, Volume 11, Issue 13, pp. 2714 - 2726
Generating a catalogue of sperm nuclear proteins is an important first step towards the clarification of the function of the paternal chromatin transmitted to...
Cell biology | Chromatin | Spermatozoa | Sperm nucleus | HUMAN SPERMATOZOA | LOCALIZATION | PROTEIN | CYTOSKELETON | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | IPG STRIP PREFRACTIONATION | HISTONE | ORGANIZATION | LC-MS/MS IDENTIFICATION | EXPRESSION | Nuclear Proteins - analysis | Humans | Male | Cell Nucleus - chemistry | Spermatozoa - cytology | Proteome - analysis | Proteomics - methods | Cell Nucleus - ultrastructure | Chromatography, Liquid - methods | Tandem Mass Spectrometry - methods | Electrophoresis, Polyacrylamide Gel - methods | Proteins | Microscopy | Sperm
Cell biology | Chromatin | Spermatozoa | Sperm nucleus | HUMAN SPERMATOZOA | LOCALIZATION | PROTEIN | CYTOSKELETON | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | IPG STRIP PREFRACTIONATION | HISTONE | ORGANIZATION | LC-MS/MS IDENTIFICATION | EXPRESSION | Nuclear Proteins - analysis | Humans | Male | Cell Nucleus - chemistry | Spermatozoa - cytology | Proteome - analysis | Proteomics - methods | Cell Nucleus - ultrastructure | Chromatography, Liquid - methods | Tandem Mass Spectrometry - methods | Electrophoresis, Polyacrylamide Gel - methods | Proteins | Microscopy | Sperm
Journal Article
Microscopy Research and Technique, ISSN 1059-910X, 04/2010, Volume 73, Issue 4, pp. 241 - 278
Spermatogenesis, a study of germ cell development, is a long, orderly, and well‐defined process occurring in seminiferous tubules of the testis. It is a...
stem cell renewal | meiosis | Sertoli | stages | synaptonemal complex | human | sex body | Human | Sex body | Stem cell renewal | Stages | Synaptonemal complex | Meiosis | C-KIT RECEPTOR | ANATOMY & MORPHOLOGY | IPG STRIP PREFRACTIONATION | TESTIS-SPECIFIC EXPRESSION | PEPTIDYL-PROLYL ISOMERASE | MICROSCOPY | MEIOTIC PROPHASE-I | PLURIPOTENT STEM-CELLS | DNA MISMATCH REPAIR | BIOLOGY | LIGAND GROWTH-FACTOR | MOUSE SERTOLI CELLS | CENTROMERE PROTEIN INCENP | Cell Proliferation | Spermatogenesis | Humans | Rats | Male | Germ Cells - growth & development | Spermatogonia - physiology | Spermatocytes - physiology | Animals | Spermatids - physiology | Germ Cells - cytology | Mice | Spermatozoa | Phases | Genes | Markers | Stem cells | Genetics | Division
stem cell renewal | meiosis | Sertoli | stages | synaptonemal complex | human | sex body | Human | Sex body | Stem cell renewal | Stages | Synaptonemal complex | Meiosis | C-KIT RECEPTOR | ANATOMY & MORPHOLOGY | IPG STRIP PREFRACTIONATION | TESTIS-SPECIFIC EXPRESSION | PEPTIDYL-PROLYL ISOMERASE | MICROSCOPY | MEIOTIC PROPHASE-I | PLURIPOTENT STEM-CELLS | DNA MISMATCH REPAIR | BIOLOGY | LIGAND GROWTH-FACTOR | MOUSE SERTOLI CELLS | CENTROMERE PROTEIN INCENP | Cell Proliferation | Spermatogenesis | Humans | Rats | Male | Germ Cells - growth & development | Spermatogonia - physiology | Spermatocytes - physiology | Animals | Spermatids - physiology | Germ Cells - cytology | Mice | Spermatozoa | Phases | Genes | Markers | Stem cells | Genetics | Division
Journal Article