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biochemical research methods (190) 190
index medicus (153) 153
prefractionation (152) 152
humans (134) 134
proteins (121) 121
proteomics (120) 120
proteomics - methods (104) 104
chemistry, analytical (103) 103
mass spectrometry (100) 100
animals (98) 98
mass-spectrometry (87) 87
biochemistry & molecular biology (73) 73
identification (72) 72
male (60) 60
sample prefractionation (60) 60
ipg strip prefractionation (57) 57
proteome (57) 57
isoelectric focusing - methods (56) 56
isoelectric focusing (51) 51
2-dimensional electrophoresis (49) 49
electrophoresis, gel, two-dimensional (49) 49
electrophoresis, gel, two-dimensional - methods (49) 49
hydrogen-ion concentration (49) 49
analysis (44) 44
proteome - analysis (43) 43
separation (43) 43
chromatography (42) 42
fractionation (40) 40
electrophoresis (38) 38
lc-ms/ms identification (37) 37
2-dimensional gel-electrophoresis (36) 36
spectrometry, mass, matrix-assisted laser desorption-ionization (35) 35
electrophoresis, polyacrylamide gel (34) 34
prefractionation techniques (33) 33
immobilized ph gradients (31) 31
spermatozoa (31) 31
two-dimensional gel electrophoresis (31) 31
proteins - isolation & purification (30) 30
mice (29) 29
proteins - analysis (29) 29
gel-electrophoresis (28) 28
proteome analysis (28) 28
tandem mass spectrometry (28) 28
chromatography, liquid (26) 26
isoelectric focusing - instrumentation (26) 26
peptides (26) 26
proteins - chemistry (24) 24
proteomic analysis (24) 24
solution isoelectrofocusing prior (24) 24
spermatozoa - metabolism (23) 23
expression (22) 22
membrane-proteins (22) 22
chemical fractionation - methods (21) 21
global analysis (21) 21
chromatography, liquid - methods (20) 20
female (20) 20
mass spectrometry - methods (20) 20
membranes (20) 20
proteome - metabolism (20) 20
purification (20) 20
liquid-chromatography (19) 19
sperm (19) 19
tandem mass-spectrometry (19) 19
chemical fractionation (18) 18
depletion (18) 18
free-flow electrophoresis (18) 18
proteome - isolation & purification (18) 18
reproducibility of results (18) 18
research (18) 18
isoelectric point (17) 17
phosphorylation (17) 17
proteome - chemistry (17) 17
rats (17) 17
article (16) 16
blood proteins - analysis (16) 16
cell biology (16) 16
reproductive biology (16) 16
albumin (15) 15
biomarkers (15) 15
blood proteins - chemistry (15) 15
human spermatozoa (15) 15
immobiline membranes (15) 15
plasma (15) 15
proteins - metabolism (15) 15
shotgun proteomics (15) 15
blood proteins - isolation & purification (14) 14
gels (14) 14
liquid chromatography (14) 14
molecular sequence data (14) 14
peptides - chemistry (14) 14
spermatozoa - chemistry (14) 14
amino acid sequence (13) 13
life sciences (13) 13
physiological aspects (13) 13
technology (13) 13
cancer (12) 12
cell line, tumor (12) 12
cells (12) 12
fertilization (12) 12
isoelectric trapping (12) 12
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1. Full Text Protein purification by Off‐Gel electrophoresis
by Ros, Alexandra and Faupel, Michel and Mees, Hervé and Oostrum, Jan van and Ferrigno, Rosaria and Reymond, Frédéric and Michel, Philippe and Rossier, Joël S and Girault, Hubert H
PROTEOMICS, ISSN 1615-9853, 02/2002, Volume 2, Issue 2, pp. 151 - 156
A novel free‐flow protein purification technique based on isoelectric electrophoresis is presented, where the proteins are purified in solution without the... 
Prefractionation | Isoelectric focusing | Isoelectric purification
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2. Full Text Tracing the origins of centrioles, cilia, and flagella
by Carvalho-Santos, Zita and Azimzadeh, Juliette and Pereira-Leal, José. B and Bettencourt-Dias, Mónica
Journal of Cell Biology, ISSN 0021-9525, 07/2011, Volume 194, Issue 2, pp. 165 - 175
Centrioles/basal bodies (CBBs) are microtubule-based cylindrical organelles that nucleate the formation of centrosomes, cilia, and flagella. CBBs, cilia, and... 
CENTROSOME BIOGENESIS | EPITHELIAL-CELLS | LENGTH CONTROL | ACCESSORY STRUCTURES | LC-MS/MS IDENTIFICATION | IPG STRIP PREFRACTIONATION | BASAL BODY | PROTEOMIC ANALYSIS | ANCESTRAL EUKARYOTE | INTRAFLAGELLAR TRANSPORT | CELL BIOLOGY | Physiological aspects | Centrioles | Cilia and ciliary motion | Flagella (Microbiology) | Reviews
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3. Full Text Affinity prefractionation for MS‐based plasma proteomics
by Pernemalm, Maria and Lewensohn, Rolf and Lehtiö, Janne
PROTEOMICS, ISSN 1615-9853, 03/2009, Volume 9, Issue 6, pp. 1420 - 1427
The plasma proteome has proven to be one of the most challenging proteomes to profile using currently available proteomics technologies. A plethora of... 
Plasma | Prefractionation | Affinity prefractionation | OXIDATIVE STRESS | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | SIALIC-ACID | HUMAN SERUM | ION-TRAP | BREAST-CANCER | HYDRAZIDE CHEMISTRY | IMMUNOAFFINITY FRACTIONATION | BIOMARKER DISCOVERY | LOW-ABUNDANCE PROTEINS | DESORPTION/IONIZATION MASS-SPECTROMETRY | Blood Proteins - analysis | Peptides - blood | Peptides - isolation & purification | Animals | Peptides - chemistry | Humans | Mass Spectrometry | Proteomics - methods | Chemical Fractionation - methods | Chromatography, Affinity - methods
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4. Plants in traditional medicinal system - Future source of new drugs
by Rout, Soumya Prakash and Choudary, K.A and Kar, D.M and Das, Lopamudra and Jain, Avijeet
International Journal of Pharmacy and Pharmaceutical Sciences, 2009, Issue 1, pp. 1 - 23
Bioprospecting | Combinational chemistry | Prefractionation | HTS | Ethnomedicine
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5. Application of feed prefractionation technology for reducing benzene in reforming gasoline
by Lang, Bao and Wang, Xuechuan and Ma, Sidan and Zhang, Ting
Petroleum Processing and Petrochemicals, ISSN 1005-2399, 11/2015, Volume 46, Issue 11, pp. 52 - 55
Benzene | Gasoiine | Reforming | Prefractionation | Optimization
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6. Full Text Prefractionation techniques in proteome analysis
by Righetti, Pier Giorgio and Castagna, Annalisa and Herbert, Ben and Reymond, Frederic and Rossier, Joël S
PROTEOMICS, ISSN 1615-9853, 08/2003, Volume 3, Issue 8, pp. 1397 - 1407
The present review deals with a number of prefractionation protocols in preparation for two‐dimensional map analysis, both in the fields of chromatography and... 
Prefractionation techniques | Proteome analyses | Review | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | SOLUTION ISOELECTROFOCUSING PRIOR | HAEMOPHILUS-INFLUENZAE | 2-DIMENSIONAL GEL-ELECTROPHORESIS | CARRIER AMPHOLYTES | MASS-SPECTROMETRY | proteome analyses | PREPARATIVE PURIFICATION | prefractionation techniques | FREE-FLOW ELECTROPHORESIS | SAMPLE PREFRACTIONATION | HEPARIN CHROMATOGRAPHY | review | IMMOBILIZED PH GRADIENTS | Electrophoresis, Gel, Two-Dimensional | Proteome | Chromatography, Liquid - methods | Chemical Fractionation
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7. Full Text New solution IEF device for micropreparative separation of peptides and proteins
by Duša, Filip and Šlais, Karel
ELECTROPHORESIS, ISSN 0173-0835, 06/2013, Volume 34, Issue 11, pp. 1519 - 1525
The article presents a new concept of preparative solution IEF where time requirements and efficiency are similar to gel‐based IEF whereas simple fraction... 
Micropreparative | Caseinomacropeptide | Whey | Solution isoelectric focusing | Bioactive peptides | CHEMISTRY, ANALYTICAL | ACIDIC PH | BIOCHEMICAL RESEARCH METHODS | 2-DIMENSIONAL ELECTROPHORESIS | PREPARATIVE ANALYSIS | AMPHOLYTIC DYES | MASS-SPECTROMETRY | FREE-FLOW ELECTROPHORESIS | SAMPLE PREFRACTIONATION | PROTEOME ANALYSIS | PREFRACTIONATION TECHNIQUES | MULTICOMPARTMENT ELECTROLYZER | Isoelectric Focusing - instrumentation | Milk Proteins - isolation & purification | Isoelectric Focusing - methods | Peptides - isolation & purification | Whey Proteins | Equipment Design | Proteins | Strip | Separation | Peptides | Focusing | Raw | Channels
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8. Full Text In-depth proteomic analysis of the human sperm reveals complex protein compositions
by Wang, Jing and Wang, Gaigai and Guo, Xuejiang and Guo, Yueshuai and Zhou, Tao and Zhou, Zuomin and Zhou, Quan and Shi, Xiaodan and Yu, Jun and Yu, Chunmei and Yang, Ye and Wu, Yibo and Liu, Mingxi and Chen, Xin and Tu, Wenjiao and Zeng, Yan and Jiang, Min and Li, Suying and Zhang, Pan and Zheng, Bo and Sha, Jiahao
Journal of Proteomics, ISSN 1874-3919, 02/2013, Volume 79, pp. 114 - 122
The male gamete (sperm) can fertilize an egg, and pass the male genetic information to the offspring. It has long been thought that sperm had a simple protein... 
Drug | Human | Proteome | Signaling pathway | Sperm | Mass spectrometry | RNA | DROSOPHILA-MELANOGASTER | BIOCHEMICAL RESEARCH METHODS | ALPHA | IPG STRIP PREFRACTIONATION | IDENTIFICATION | ORIGIN | CAPACITATION | EXPRESSION | ACROSOME REACTION | MOTILITY | Bone morphogenetic proteins | Spermatozoa | Analysis
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9. Full Text State of the Human Proteome in 2013 as Viewed through PeptideAtlas: Comparing the Kidney, Urine, and Plasma Proteomes for the Biology- and Disease-Driven Human Proteome Project
by Farrah, Terry and Deutsch, Eric W and Omenn, Gilbert S and Sun, Zhi and Watts, Julian D and Yamamoto, Tadashi and Shteynberg, David and Harris, Micheleen M and Moritz, Robert L
Journal of Proteome Research, ISSN 1535-3893, 01/2014, Volume 13, Issue 1, pp. 60 - 75
The kidney, urine, and plasma proteomes are intimately related: proteins and metabolic waste products are filtered from the plasma by the kidney and excreted... 
kidney | LC-MS/MS | database | urine | PeptideAtlas | Human Proteome Project | plasma | proteome comparison | TANDEM | PREFRACTIONATION | BIOCHEMICAL RESEARCH METHODS | IDENTIFICATION | COMBINATION | ANNOTATION | EPIDERMAL-GROWTH-FACTOR | HIGH-CONFIDENCE | PROTEINS | EXPRESSION | Chromatography, Liquid | Tandem Mass Spectrometry | Proteome | Proteins - metabolism | Humans | LC-MS
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10. Full Text New insights into epididymal function in relation to sperm maturation
by Dacheux, Jean-Louis and Dacheux, Françoise
Reproduction, ISSN 1470-1626, 02/2014, Volume 147, Issue 2, pp. R27 - R42
Testicular spermatozoa acquire fertility only after 1 or 2 weeks of transit through the epididymis. At the end of this several meters long epididymal tubule,... 
CARBONIC-ANHYDRASE-XIV | REPRODUCTIVE BIOLOGY | PROTEIN PHOSPHATASE 1 | ZONA-PELLUCIDA-BINDING | SOLUBLE ADENYLYL-CYCLASE | TYROSINE PHOSPHORYLATION | MOUSE SPERM | LC-MS/MS IDENTIFICATION | IPG STRIP PREFRACTIONATION | DEVELOPMENTAL BIOLOGY | PROTEOMIC ANALYSIS | MAMMALIAN SPERMATOZOA | Proteins - physiology | Spermatozoa - chemistry | Phosphorylation | Humans | Male | Proteins - analysis | Epididymis - physiology | Sperm Transport - physiology | Sperm Capacitation - physiology | Epididymis - chemistry | Sperm-Ovum Interactions | Animals | Calcium - physiology | Sperm Motility - physiology | Fertility | Proteomics | Bicarbonates - metabolism | Spermatozoa - physiology | Sperm Maturation - physiology | Life Sciences | Reproductive Biology | Other
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11. Full Text Proteome of cat semen obtained after urethral catheterization
by Mogielnicka-Brzozowska, Marzena and Prochowska, Sylwia and Niżański, Wojciech and Bromke, Mariusz A and Wiśniewski, Jerzy and Olejnik, Beata and Kuzborska, Anna and Fraser, Leyland and Młynarz, Piotr and Kordan, Władysław
Theriogenology, ISSN 0093-691X, 01/2020, Volume 141, pp. 68 - 81
The binding of seminal plasma (SP) proteins by spermatozoa plays an important role in the regulation of sperm epididymal maturation, motility gaining in female... 
Proteins | Spermatozoa | Seminal plasma | Proteomics | Cat | PROLACTIN-INDUCIBLE PROTEIN | HUMAN SERUM-ALBUMIN | GLUTATHIONE-PEROXIDASE | SPERM PROTEOME | SEMINAL PLASMA-PROTEINS | IPG STRIP PREFRACTIONATION | MAIN PROTEIN | FERTILITY | REPRODUCTIVE BIOLOGY | VETERINARY SCIENCES | RAT SPERM | BINDING
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12. Full Text A comprehensive analysis and annotation of human normal urinary proteome
by Zhao, Mindi and Li, Menglin and Yang, Yehong and Guo, Zhengguang and Sun, Ying and Shao, Chen and Li, Mingxi and Sun, Wei and Gao, Youhe
Scientific Reports, ISSN 2045-2322, 12/2017, Volume 7, Issue 1, pp. 3024 - 13
Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic... 
ACUTE KIDNEY INJURY | GLOMERULAR-BASEMENT-MEMBRANE | DIFFERENTIAL EXPRESSION | BIOMARKER DISCOVERY | MULTIDISCIPLINARY SCIENCES | NON-PREFRACTIONATION | DIABETIC-NEPHROPATHY | TANDEM MASS-SPECTROMETRY | ACUTE RENAL INJURY | LARGE GENE LISTS | FOCAL SEGMENTAL GLOMERULOSCLEROSIS | Tandem Mass Spectrometry | Computational Biology - methods | Biomarkers - urine | Databases, Protein | Humans | Molecular Sequence Annotation | Chromatography, Liquid | Proteome | Chromatography, High Pressure Liquid | Proteomics - methods | Urine | Proteins | Mass spectroscopy | Liquid chromatography | Isoelectric focusing
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13. Full Text Proteomic characterization of the human sperm nucleus
by de Mateo, Sara and Castillo, Judit and Estanyol, Josep Maria and Ballescà, José Luis and Oliva, Rafael
PROTEOMICS, ISSN 1615-9853, 07/2011, Volume 11, Issue 13, pp. 2714 - 2726
Generating a catalogue of sperm nuclear proteins is an important first step towards the clarification of the function of the paternal chromatin transmitted to... 
Cell biology | Chromatin | Spermatozoa | Sperm nucleus | HUMAN SPERMATOZOA | LOCALIZATION | PROTEIN | CYTOSKELETON | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | IPG STRIP PREFRACTIONATION | HISTONE | ORGANIZATION | LC-MS/MS IDENTIFICATION | EXPRESSION | Nuclear Proteins - analysis | Humans | Male | Cell Nucleus - chemistry | Spermatozoa - cytology | Proteome - analysis | Proteomics - methods | Cell Nucleus - ultrastructure | Chromatography, Liquid - methods | Tandem Mass Spectrometry - methods | Electrophoresis, Polyacrylamide Gel - methods | Proteins | Microscopy | Sperm
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14. Full Text Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: Background to spermatogenesis, spermatogonia, and spermatocytes
by Hermo, Louis and Pelletier, R.‐Marc and Cyr, Daniel G and Smith, Charles E and Pelletier, R.‐Marc
Microscopy Research and Technique, ISSN 1059-910X, 04/2010, Volume 73, Issue 4, pp. 241 - 278
Spermatogenesis, a study of germ cell development, is a long, orderly, and well‐defined process occurring in seminiferous tubules of the testis. It is a... 
stem cell renewal | meiosis | Sertoli | stages | synaptonemal complex | human | sex body | Human | Sex body | Stem cell renewal | Stages | Synaptonemal complex | Meiosis | C-KIT RECEPTOR | ANATOMY & MORPHOLOGY | IPG STRIP PREFRACTIONATION | TESTIS-SPECIFIC EXPRESSION | PEPTIDYL-PROLYL ISOMERASE | MICROSCOPY | MEIOTIC PROPHASE-I | PLURIPOTENT STEM-CELLS | DNA MISMATCH REPAIR | BIOLOGY | LIGAND GROWTH-FACTOR | MOUSE SERTOLI CELLS | CENTROMERE PROTEIN INCENP | Cell Proliferation | Spermatogenesis | Humans | Rats | Male | Germ Cells - growth & development | Spermatogonia - physiology | Spermatocytes - physiology | Animals | Spermatids - physiology | Germ Cells - cytology | Mice | Spermatozoa | Phases | Genes | Markers | Stem cells | Genetics | Division
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