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1. Real-time PCR
: advanced technologies and applications
by Saunders, Nick A and Lee, Martin A
2013, ISBN 1908230223, vi, 283 p., A9
Polymerase chain reaction | Methodology
Book
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2. Real-time PCR
: current technology and applications
by Logan, Julie and Edwards, Kirstin and Saunders, Nick
2009, ISBN 1904455395, vii, 284 p., [4] p. of plates
Polymerase chain reaction
Book
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3. Real-time PCR
by Dorak, M. Tevfik
2006, Advanced Methods, ISBN 9780415377348, xxvi, 333
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these... 
Polymerase chain reaction | Réaction en chaîne de la polymérase | Biology, life sciences | Biotechnology | Mathematical Biology | Polymerase Chain Reaction - methods
Book
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4. Real-time PCR
: an essential guide
by Saunders, Nick (Nick A.) and Logan, Julie (Julie M. J.) and Edwards, Kirstin (Kirstin J.)
2004, ISBN 095452327X, vi, 346
Polymerase chain reaction | Réaction en chaîne de la polymérase | Méthodes | Polymerase chain reaction -- Methods
Book
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5. Full Text Absolute quantification by droplet digital PCR versus analog real-time PCR
by Hindson, Christopher M and Chevillet, John R and Briggs, Hilary A and Gallichotte, Emily N and Ruf, Ingrid K and Hindson, Benjamin J and Vessella, Robert L and Tewari, Muneesh
Nature Methods, ISSN 1548-7091, 10/2013, Volume 10, Issue 10, pp. 1003 - 1005
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of... 
DNA COPY NUMBER | RNA | QUANTITATION | BIOCHEMICAL RESEARCH METHODS | SERUM | MICRORNAS | CANCER | Limit of Detection | Reproducibility of Results | Biomarkers, Tumor - analysis | Humans | Real-Time Polymerase Chain Reaction - methods | Male | Real-Time Polymerase Chain Reaction - standards | Prostatic Neoplasms - diagnosis | Biomarkers, Tumor - blood | MicroRNAs - analysis | Sensitivity and Specificity | Prostatic Neoplasms - blood | MicroRNAs - blood | Polymerase chain reaction | Comparative analysis | Methods | Quantitative genetics | Biomarkers | Gene expression | MicroRNAs | Index Medicus
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6. Full Text Cross-platform comparison of SYBR® Green real-time PCR with TaqMan PCR, microarrays and other gene expression measurement technologies evaluated in the MicroArray Quality Control (MAQC) study
by Arikawa, Emi and Sun, Yanyang and Wang, Jie and Zhou, Qiong and Ning, Baitang and Dial, Stacey L and Guo, Lei and Yang, Jingping
BMC Genomics, ISSN 1471-2164, 07/2008, Volume 9, Issue 1, pp. 328 - 328
Background: The MicroArray Quality Control (MAQC) project evaluated the inter-and intra-platform reproducibility of seven microarray platforms and three... 
PROJECT | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | DNA MICROARRAY | PERFORMANCE | GENETICS & HEREDITY | QUANTITATIVE PCR | ARRAYS | Reproducibility of Results | Oligonucleotide Array Sequence Analysis - standards | RNA - analysis | Oligonucleotide Array Sequence Analysis - methods | Humans | Organic Chemicals - chemistry | Gene Expression Profiling - methods | Polymerase Chain Reaction - methods | Reference Standards | Sensitivity and Specificity | Polymerase Chain Reaction - standards | Gene Expression Profiling - standards | Quality Control | Index Medicus
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7. Full Text Performance of a Real-time PCR–based Approach and Droplet Digital PCR in Detecting Human Parechovirus Type 3 RNA
by Aizawa, Yuta and Koyama, Akihide and Ishihara, Tomohiko and Onodera, Osamu and Saitoh, Akihiko, M.D., Ph.D
Journal of Clinical Virology, ISSN 1386-6532, 2016, Volume 84, pp. 27 - 31
Highlights • Real-time RT-PCR detection limits for HPeV3 have not been fully evaluated. • RT-ddPCR is the novel technique with precise quantitation of low-copy... 
Infectious Disease | Allergy and Immunology | Neonates | Digital PCR | Human parechovirus type 3 | Infants | Cerebrospinal fluid | Real-time PCR | QUANTITATIVE DETECTION | VIRUS | YOUNG INFANTS | CHILDREN | ENTEROVIRUS | VIROLOGY | ENCEPHALITIS | NERVOUS-SYSTEM INFECTIONS | SAMPLES | DIAGNOSTICS | Limit of Detection | Parechovirus - genetics | Meningoencephalitis - diagnosis | Humans | Real-Time Polymerase Chain Reaction - methods | Sepsis - diagnosis | Genotype | Infant | Male | RNA, Viral - blood | Parechovirus - isolation & purification | Picornaviridae Infections - diagnosis | Picornaviridae Infections - virology | Viral Load | Sepsis - virology | RNA, Viral - genetics | Meningoencephalitis - virology | Female | RNA, Viral - cerebrospinal fluid | RNA, Viral - isolation & purification | Infant, Newborn | RNA | Neurosciences | Infants (Newborn) | Medicine, Experimental | Medical research | Health aspects | Analysis | Index Medicus
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8. Real-time PCR in microbiology
: from diagnosis to characterisation
by Mackay, Ian M
2007, ISBN 1904455182, x, 454
Polymerase chain reaction | Medical microbiology | Molecular diagnosis
Book
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9. Full Text Linear-after-the-Exponential (LATE)-PCR: An Advanced Method of Asymmetric PCR and Its Uses in Quantitative Real-Time Analysis
by J. Aquiles Sanchez and Kenneth E. Pierce and John E. Rice and Lawrence J. Wangh and Gregory A. Petsko
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 2/2004, Volume 101, Issue 7, pp. 1933 - 1938
Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the... 
Polymerase chain reaction | Biological Sciences | Annealing | Melting | DNA probes | Beacons | Temperature probes | Alleles | Kinetics | Signal detection | Signal amplification | CELLS | THERMODYNAMICS | AMPLIFICATION | MULTIDISCIPLINARY SCIENCES | POLYMERASE CHAIN-REACTION | MOLECULAR BEACONS | HYBRIDIZATION | MISMATCHES | SINGLE-STRANDED-DNA | MUTATIONS | PROBES | Cell Line | Reproducibility of Results | Temperature | Time Factors | Cystic Fibrosis - genetics | Humans | Sensitivity and Specificity | DNA Primers - genetics | Research Design | Tay-Sachs Disease - genetics | Polymerase Chain Reaction - methods | Cells | Research | Quantitative genetics | Real time | Analysis | Methods | Index Medicus
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10. Full Text Development of a rapid PCR-Nucleic Acid Lateral Flow Immunoassay (PCR-NALFIA) based on rDNA IGS sequence analysis for the detection of Macrophomina phaseolina in soil
by Pecchia, Susanna and Da Lio, Daniele
Journal of Microbiological Methods, ISSN 0167-7012, 08/2018, Volume 151, pp. 118 - 128
The ‘ ’ (NALFIA) using a generic ‘ ’ (LFD), combined with PCR employing labelled primers (PCR-NALFIA), enables to circumvent the use of electrophoresis, making... 
rDNA-IGS region | Nucleic acid lateral flow immunoassay (NALFIA) | Molecular diagnosis | Soil-borne pathogen | Botryosphaeriaceae | PCR | MICROBIAL DNA | BOTRYTIS-CINEREA | INTERGENIC SPACER REGION | FUNGAL PATHOGENS | BIOCHEMICAL RESEARCH METHODS | MICROBIOLOGY | NATURAL SOILS | TIME QUANTITATIVE PCR | DRAFT GENOME SEQUENCES | VERTICILLIUM-DAHLIAE | FUSARIUM-GRAMINEARUM | RIBOSOMAL-DNA | DNA, Intergenic | Ascomycota - isolation & purification | Molecular Diagnostic Techniques - methods | Species Specificity | Soil Microbiology | DNA Primers - genetics | DNA, Fungal | Nucleic Acids | Polymerase Chain Reaction - methods | Sensitivity and Specificity | Ascomycota - genetics | Soil - chemistry | Immunoassay - methods | Sequence Analysis | DNA, Ribosomal - genetics | Analysis | Nucleic acids
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11. A-Z of quantitative PCR
by Bustin, Stephen A
2004, IUL biotechnology series, ISBN 9780963681782, Volume 5., xxix, 882
Polymerase chain reaction | Quantitative genetics | Réaction en chaîne de la polymérase | Analyse | Acides nucléiques | Génétique quantitative | Analysis | polynumerase chain reaction | Nucleic acids | Nucleic acids -- Analysis
Book
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12. Full Text Minimal Residual Disease Detection by Droplet Digital PCR in Multiple Myeloma, Mantle Cell Lymphoma, and Follicular Lymphoma: A Comparison with Real-Time PCR
by Drandi, Daniela and Kubiczkova-Besse, Lenka and Ferrero, Simone and Dani, Nadia and Passera, Roberto and Mantoan, Barbara and Gambella, Manuela and Monitillo, Luigia and Saraci, Elona and Ghione, Paola and Genuardi, Elisa and Barbero, Daniela and Omedè, Paola and Barberio, Davide and Hajek, Roman and Vitolo, Umberto and Palumbo, Antonio and Cortelazzo, Sergio and Boccadoro, Mario and Inghirami, Giorgio and Ladetto, Marco
Journal of Molecular Diagnostics, ISSN 1525-1578, 11/2015, Volume 17, Issue 6, pp. 652 - 660
Real-time quantitative PCR (qPCR) is a well-established tool for minimal residual disease (MRD) detection in mature lymphoid malignancies. Despite remarkable... 
CONSOLIDATION | POLYMERASE-CHAIN-REACTION | DNA COPY NUMBER | MULTIPARAMETER FLOW-CYTOMETRY | QUANTIFICATION | BONE-MARROW | QUANTITATIVE PCR | MALIGNANCIES | PATHOLOGY | REARRANGEMENTS | TRANSPLANTATION | Lymphoma, Mantle-Cell - diagnosis | Reference Standards | Reproducibility of Results | Multiple Myeloma - diagnosis | Humans | Neoplasm, Residual - diagnosis | Sensitivity and Specificity | Lymphoma, Follicular - diagnosis | Real-Time Polymerase Chain Reaction - methods | Index Medicus
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13. Full Text Reference genes in real-time PCR
by Kozera, Bartłomiej and Rapacz, Marcin
Journal of Applied Genetics, ISSN 1234-1983, 11/2013, Volume 54, Issue 4, pp. 391 - 406
This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are... 
Life Sciences | Human Genetics | Housekeeping genes | Validation | Reference gene | Life Sciences, general | Microbial Genetics and Genomics | Selection | Gene expression | Animal Genetics and Genomics | Plant Genetics & Genomics | PHOTOSYNTHETIC APPARATUS | EXPRESSION ANALYSIS | SYSTEMATIC VALIDATION | INTERNAL CONTROL | REVERSE TRANSCRIPTION | QUANTITATIVE RT-PCR | POLYMERASE-CHAIN-REACTION | MESSENGER-RNA | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | ENDOGENOUS REFERENCE GENES | Reproducibility of Results | Genes, Plant | RNA, Ribosomal, 18S - genetics | Gene Expression Regulation | Real-Time Polymerase Chain Reaction - methods | Selection, Genetic | Plants - classification | Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - genetics | Genetic Variation | Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism | Reference Standards | Algorithms | Plants - genetics | Gene Expression Regulation, Plant | RNA, Ribosomal, 18S - metabolism | Plant Genetics • Review
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14. Full Text Statistical analysis of real-time PCR data
by Yuan, Joshua S and Reed, Ann and Chen, Feng and Stewart Jr, C. Neal
BMC Bioinformatics, ISSN 1471-2105, 02/2006, Volume 7, Issue 1, pp. 85 - 85
Background: Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time... 
BIOTECHNOLOGY & APPLIED MICROBIOLOGY | QUANTIFICATION | TRANSCRIPTION | BIOCHEMICAL RESEARCH METHODS | MATHEMATICAL & COMPUTATIONAL BIOLOGY | QUANTITATIVE RT-PCR | EXPRESSION | DNA Probes | Computational Biology - methods | Data Interpretation, Statistical | Reproducibility of Results | Oligonucleotide Array Sequence Analysis | Humans | Base Pair Mismatch | Gene Expression Profiling | Statistics as Topic | Models, Statistical | RNA - chemistry | Regression Analysis | Animals | Analysis of Variance | Models, Biological | Reverse Transcriptase Polymerase Chain Reaction - methods | Sensitivity and Specificity | Cloning, Molecular | Polymerase Chain Reaction | Programming Languages | Mice | Software | DNA, Complementary | Quality Control | Index Medicus
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15. Full Text Comparative Evaluation Between the RealStar Pneumocystis jirovecii PCR Kit and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR Kit for Detecting P. jirovecii in Non-HIV Immunocompromised Patients
by Huh, Hee Jae and Huh, Hee Jae and Lim, Kyoung Ree and Ki, Chang Seok and Ki, Chang-Seok and Huh, Kyungmin and Huh, Kyungmin and Shim, Hyang Jin and Song, Dong Joon and Song, Dong Joon and Kim, Yae-Jean and Kim, Yae Jean and Chung, Doo Ryeon and Chung, Doo Ryeon and Lee, Nam Yong and Lee, Nam Yong
Annals of laboratory medicine, ISSN 2234-3806, 03/2019, Volume 39, Issue 2, pp. 176 - 182
Background: Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for... 
RealStar Pneumocystis jirovecii PCR | Real-time PCR | Performance | AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR | Pneumocystis jirovecii | Pneumocystis pneumonia | DIAGNOSIS | ASSAY | MANAGEMENT | TIME PCR | QUANTITATIVE PCR | COLONIZATION | CARINII | PNEUMONIA | LAVAGE SPECIMENS | DIFFERENTIATION | MEDICAL LABORATORY TECHNOLOGY | Index Medicus | Original
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16. Full Text Comparative analysis between RQ‐PCR and digital‐droplet‐PCR of immunoglobulin/T‐cell receptor gene rearrangements to monitor minimal residual disease in acute lymphoblastic leukaemia
by Della Starza, Irene and Nunes, Vittorio and Cavalli, Marzia and De Novi, Lucia Anna and Ilari, Caterina and Apicella, Valerio and Vitale, Antonella and Testi, Anna Maria and Del Giudice, Ilaria and Chiaretti, Sabina and Foà, Robin and Guarini, Anna
British Journal of Haematology, ISSN 0007-1048, 08/2016, Volume 174, Issue 4, pp. 541 - 549
Real‐time quantitative polymerase chain reaction ( RQ ‐ PCR ) is a standardized tool for minimal residual disease ( MRD ) monitoring in acute lymphoblastic... 
ALL | gene rearrangement | quantitative | PCR | MRD | Quantitative PCR | Gene rearrangement | quantitative PCR | RISK | CLASSIFICATION | TIME QUANTITATIVE PCR | CHILDHOOD | DNA COPY NUMBER | CONCERTED ACTION | TRANSCRIPT | ABSOLUTE QUANTIFICATION | HEMATOLOGY | CLINICAL-SIGNIFICANCE | Clinical Laboratory Techniques | Humans | Polymerase Chain Reaction - methods | Genes, Immunoglobulin | Young Adult | Gene Rearrangement | Neoplasm, Residual - diagnosis | Sensitivity and Specificity | Adolescent | Polymerase Chain Reaction - standards | Adult | Genes, T-Cell Receptor | Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology | Methods | Genetic research | Comparative analysis | Genes | Index Medicus
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