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index medicus (277) 277
rna-guided endonuclease (275) 275
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humans (130) 130
cas9 (124) 124
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crispr-cas systems (109) 109
genomes (109) 109
genomics (106) 106
biochemistry & molecular biology (103) 103
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genome editing (86) 86
zinc-finger nucleases (86) 86
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genome (70) 70
homologous recombination (69) 69
crispr/cas9 (66) 66
crispr-cas systems - genetics (65) 65
article (63) 63
cell biology (60) 60
deoxyribonucleic acid--dna (59) 59
biotechnology & applied microbiology (58) 58
gene editing (58) 58
dna (57) 57
in-vivo (57) 57
mutation (56) 56
gene editing - methods (54) 54
rna-guided endonucleases (54) 54
mutagenesis (50) 50
proteins (50) 50
targeted mutagenesis (50) 50
genes (48) 48
plant sciences (48) 48
rna (48) 48
analysis (47) 47
pluripotent stem-cells (44) 44
research (44) 44
rna, guide - genetics (44) 44
genetics & heredity (43) 43
clustered regularly interspaced short palindromic repeats (42) 42
base sequence (41) 41
endonucleases - metabolism (41) 41
gene targeting (40) 40
gene-expression (40) 40
crispr-cas9 (39) 39
multidisciplinary sciences (39) 39
clustered regularly interspaced short palindromic repeats - genetics (38) 38
genetic engineering - methods (38) 38
genetic research (38) 38
methods (38) 38
specificity (38) 38
double-strand breaks (37) 37
system (37) 37
endonucleases - genetics (34) 34
gene expression (34) 34
genetic aspects (34) 34
biotechnology (33) 33
gene therapy (33) 33
biochemical research methods (32) 32
cpf1 (32) 32
dna cleavage (32) 32
plants (32) 32
one-step generation (31) 31
review (31) 31
cleavage (30) 30
ribonucleic acid--rna (30) 30
crystal-structure (29) 29
editing (29) 29
genetics (29) 29
enzymes (28) 28
escherichia-coli (28) 28
gene (28) 28
mice (28) 28
cas system (27) 27
bacterial proteins - genetics (26) 26
hepatitis-b-virus (26) 26
microbiology (26) 26
bacterial proteins - metabolism (25) 25
crispr-associated proteins - genetics (25) 25
dna binding proteins (25) 25
rna, guide - metabolism (25) 25
transcription (25) 25
structural basis (24) 24
arabidopsis (23) 23
endonuclease (23) 23
cas systems (22) 22
crispr-associated proteins - metabolism (22) 22
genome engineering (22) 22
immune-system (22) 22
nucleotide sequence (22) 22
sequence-specific control (22) 22
streptococcus-thermophilus (22) 22
biology (21) 21
mammalian-cells (21) 21
molecular sequence data (21) 21
plant genetics (21) 21
staphylococcus-aureus cas9 (21) 21
complex (20) 20
dna sequencing (20) 20
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1. Full Text Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
by Stella, S and Alcon, P and Montoya, G
NATURE, ISSN 0028-0836, 06/2017, Volume 546, Issue 7659, pp. 559 - 559
Cpf1 is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool(1,)2. Here we provide insight into its DNA-targeting mechanism by... 
REFINEMENT | SYSTEM | BACTERIA | SPECIFICITIES | RECOGNITION | MODELS | MULTIDISCIPLINARY SCIENCES | RNA-GUIDED ENDONUCLEASE | PHENIX | CAS9 | CRISPR/CAS | RNA, Guide - genetics | RNA, Guide - metabolism | Models, Molecular | Substrate Specificity | Crystallography, X-Ray | Gram-Positive Bacteria - enzymology | Acidaminococcus - enzymology | DNA - metabolism | Endonucleases - metabolism | Gene Editing | Endonucleases - chemistry | DNA - genetics | Base Pairing | Adenosine Triphosphate - metabolism | Francisella - enzymology | Protein Engineering | Protein Domains | Lysine - metabolism | DNA Cleavage | Methods | Nucleotide sequencing | DNA sequencing
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2. Full Text Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells
by Kim, Daesik and Kim, Jungeun and Hur, Junho K and Been, Kyung Wook and Yoon, Sun-Heui and Kim, Jin-Soo
Nature Biotechnology, ISSN 1087-0156, 01/2016, Volume 34, Issue 8, pp. 863 - 868
Programmable clustered regularly interspaced short palindromic repeats (CRISPR) Cpf1 endonucleases are single-RNA-guided (crRNA) enzymes that recognize... 
TALENS | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | OFF-TARGET CLEAVAGE | RIBONUCLEOPROTEINS | DIGENOME-SEQ | SITES | RNA-GUIDED ENDONUCLEASES | CRISPR-CAS SYSTEM | VECTORS | NUCLEASES | Clustered Regularly Interspaced Short Palindromic Repeats - physiology | Endonucleases - genetics | Humans | Bacterial Proteins - genetics | Substrate Specificity | Base Pair Mismatch | Endonucleases - metabolism | CRISPR-Cas Systems - genetics | Genome, Human - genetics | Chromosome Mapping - methods | Protein Binding | Bacterial Proteins - metabolism | Enzyme Activation | Binding Sites
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3. Full Text Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity
by Stella, Stefano and Mesa, Pablo and Thomsen, Johannes and Paul, Bijoya and Alcón, Pablo and Jensen, Simon B and Saligram, Bhargav and Moses, Matias E and Hatzakis, Nikos S and Montoya, Guillermo
Cell, ISSN 0092-8674, 12/2018, Volume 175, Issue 7, pp. 1856 - 1871.e21
Cas12a, also known as Cpf1, is a type V-A CRISPR-Cas RNA-guided endonuclease that is used for genome editing based on its ability to generate specific dsDNA... 
CRISPR-Cas | single-molecule FRET | genome editing | protein-RNA-DNA complex | cryo-EM | CYTOCHROME-P450 OXIDOREDUCTASE | COMPLEX | STATES | SINGLE | BIOCHEMISTRY & MOLECULAR BIOLOGY | CPF1 | RNA-GUIDED ENDONUCLEASE | CRYO-EM | MICROSCOPY | CLEAVAGE | CELL BIOLOGY | Thermodynamics | Enzymes | Nucleases | RNA | Analysis | Genomics | Catalysis
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4. Full Text Salient Features of Endonuclease Platforms for Therapeutic Genome Editing
by Certo, Michael T and Morgan, Richard A
Molecular Therapy, ISSN 1525-0016, 03/2016, Volume 24, Issue 3, pp. 422 - 429
Emerging gene-editing technologies are nearing a revolutionary phase in genetic medicine: precisely modifying or repairing causal genetic defects. This may... 
MEDICINE, RESEARCH & EXPERIMENTAL | HUMAN-CELLS | DOUBLE-STRAND BREAKS | ZINC-FINGER NUCLEASES | TAL-EFFECTOR NUCLEASES | OFF-TARGET MUTATIONS | CRISPR-CAS NUCLEASES | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | IN-VIVO | GENETICS & HEREDITY | HOMOLOGY-DIRECTED REPAIR | RNA-GUIDED ENDONUCLEASE | HOMING ENDONUCLEASES | Genetic Engineering - methods | Gene Transfer Techniques | Transduction, Genetic | History, 20th Century | Humans | Endonucleases - metabolism | Genetic Vectors - genetics | Gene Editing - history | Animals | Gene Editing - methods | Genetic Engineering - history | Genome | Genetic Therapy - methods | Review
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5. Full Text Detection of treatment-resistant infectious HIV after genome-directed antiviral endonuclease therapy
by De Silva Feelixge, Harshana S and Stone, Daniel and Pietz, Harlan L and Roychoudhury, Pavitra and Greninger, Alex L and Schiffer, Joshua T and Aubert, Martine and Jerome, Keith R
Antiviral Research, ISSN 0166-3542, 02/2016, Volume 126, pp. 90 - 98
Incurable chronic viral infections are a major cause of morbidity and mortality worldwide. One potential approach to cure persistent viral infections is via... 
Resistance | Reverse transcriptase | Zinc finger nuclease | Endonuclease | HEPATITIS-B-VIRUS | HUMAN-PAPILLOMAVIRUS | DRUG-RESISTANCE | HUMAN T-CELLS | IN-VITRO | VIROLOGY | CRISPR/CAS9 SYSTEM | CERVICAL-CANCER CELLS | RNA-GUIDED ENDONUCLEASE | PHARMACOLOGY & PHARMACY | PROVIRAL DNA | ZINC-FINGER-NUCLEASES | Anti-HIV Agents - pharmacology | Phosphoproteins - pharmacology | Humans | Drug Resistance, Viral | HIV Protease - genetics | Molecular Sequence Data | Reverse Transcriptase Inhibitors - pharmacology | HIV Reverse Transcriptase - genetics | Exodeoxyribonucleases - pharmacology | Endonucleases - metabolism | Phosphoproteins - metabolism | Gene Products, pol - genetics | Base Sequence | HEK293 Cells | Cell Line | Gene Products, pol - metabolism | Virus Replication - drug effects | Zinc Fingers | Transduction, Genetic | HIV-1 - drug effects | HIV Infections - genetics | HIV Infections - virology | HIV-1 - genetics | HIV Reverse Transcriptase - metabolism | Exodeoxyribonucleases - metabolism | HIV Infections - therapy | HIV Infections - drug therapy | Mutation | DNA, Viral - genetics | HIV Protease - metabolism | Antiviral agents | Nucleases | Genomics | Mortality | T cells | HIV (Viruses) | Virus diseases | Proteases | HIV testing | DNA polymerases | Zinc finger proteins | Drug therapy | Health aspects | endonuclease | zinc finger nuclease | reverse transcriptase | resistance
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6. Full Text RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?
by Gasiunas, Giedrius and Siksnys, Virginijus
Trends in Microbiology, ISSN 0966-842X, 2013, Volume 21, Issue 11, pp. 562 - 567
Highlights • Cas9 functions as an RNA-directed double-stranded DNA endonuclease. • Cas9 is easily programmable using customizable RNAs. • Cas9 is a promising... 
Internal Medicine | gene targeting | RNA-guided DNA cleavage | molecular tools | clustered regularly interspaced short palindromic repeat (CRISPR) | Molecular tools | Gene targeting | Clustered regularly interspaced short palindromic repeat (CRISPR) | RECOMBINATION | SPECIFICITY | BIOCHEMISTRY & MOLECULAR BIOLOGY | NEISSERIA-MENINGITIDIS | MICROBIOLOGY | ZINC-FINGER NUCLEASES | STREPTOCOCCUS-THERMOPHILUS | CLEAVAGE DOMAIN | IMMUNE-SYSTEM | HOMING ENDONUCLEASES | TRIPLEX-FORMING OLIGONUCLEOTIDE | RESTRICTION-ENDONUCLEASE | Genetic Engineering - methods | Recombinant Proteins - metabolism | Deoxyribonuclease I - metabolism | Recombination, Genetic | Clustered Regularly Interspaced Short Palindromic Repeats | Recombinant Proteins - genetics | DNA - metabolism | Molecular Biology - methods | RNA - metabolism | Nucleases | RNA | Genomics | DNA
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7. Full Text Bacterial CRISPR/Cas DNA endonucleases: A revolutionary technology that could dramatically impact viral research and treatment
by Kennedy, Edward M and Cullen, Bryan R
Virology, ISSN 0042-6822, 2015, Volume 479, pp. 213 - 220
Abstract CRISPR/Cas systems mediate bacterial adaptive immune responses that evolved to protect bacteria from bacteriophage and other horizontally transmitted... 
Infectious Disease | CRISPR/Cas | Gene editing | Antiviral gene therapy | Sequence specific DNA cleavage | HEPATITIS-B-VIRUS | THERAPEUTIC STRATEGY | HUMAN-CELLS | GENE-REGULATION | HUMAN-PAPILLOMAVIRUS | ZINC-FINGER NUCLEASES | ADAPTIVE IMMUNITY | VIROLOGY | IN-VIVO | RNA-GUIDED ENDONUCLEASE | EFFECTOR NUCLEASES | Recombinant Proteins - metabolism | Dependovirus - genetics | Humans | Bacterial Proteins - genetics | Recombinant Proteins - genetics | Bacteria - genetics | Molecular Biology - methods | Host-Pathogen Interactions | Endodeoxyribonucleases - genetics | Clustered Regularly Interspaced Short Palindromic Repeats | Endodeoxyribonucleases - metabolism | Bacteria - enzymology | Bacterial Proteins - metabolism | Genetic Vectors | Proteins | Genetic research | Molecular genetics | Bacterial genetics | Analysis | DNA | HUMAN POPULATIONS | BACTERIOPHAGES | GENE THERAPY | MUTAGENESIS | RNA | HERPES SIMPLEX | PHENOTYPE | IN VIVO | ANIMAL CELLS | CLEAVAGE | 60 APPLIED LIFE SCIENCES | INACTIVATION | STREPTOCOCCUS | ENDONUCLEASES | HEPATITIS | GENES | SCREENING | CRISPR | Cas
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8. Full Text Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9
by Yang, Hui and Patel, Dinshaw J and Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Molecular Cell, ISSN 1097-2765, 07/2017, Volume 67, Issue 1, pp. 117 - 127.e5
Prokaryotic CRISPR-Cas adaptive immune systems utilize sequence-specific RNA-guided endonucleases to defend against infection by viruses, bacteriophages, and... 
endonuclease | genome editing | trans-activating crRNA | CRISPR RNA | target DNA cleavage | RuvC | CRISPR-Cas | Cas9 | anti-CRISPR protein | AcrIIA4 | COMPLEX | BACTERIOPHAGE | STRUCTURAL BASIS | DNA RECOGNITION | BIOCHEMISTRY & MOLECULAR BIOLOGY | BACTERIAL IMMUNE-SYSTEM | RESISTANCE | RNA-GUIDED ENDONUCLEASE | CAS SYSTEMS | PROTEINS | CLEAVAGE | CELL BIOLOGY | CRISPR-Associated Proteins - chemistry | Endonucleases - genetics | RNA, Guide - genetics | RNA, Guide - metabolism | Bacterial Proteins - chemistry | Structure-Activity Relationship | Endonucleases - metabolism | RNA, Guide - chemistry | Clustered Regularly Interspaced Short Palindromic Repeats | Escherichia coli - metabolism | Nucleic Acid Conformation | Binding Sites | Binding, Competitive | CRISPR-Associated Proteins - genetics | Bacterial Proteins - genetics | Models, Molecular | DNA - metabolism | Gene Editing | Endonucleases - chemistry | DNA - genetics | DNA - chemistry | Escherichia coli - genetics | CRISPR-Associated Proteins - metabolism | CRISPR-Cas Systems | Protein Binding | Bacterial Proteins - metabolism | Protein Conformation | Mutation | Proteins | Nucleases | RNA | Genomics | Crystals | Structure | Health aspects | DNA
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9. Full Text Engineered CRISPR-Cas9 nuclease with expanded targeting space
by Nishimasu, Hiroshi and Shi, Xi and Ishiguro, Soh and Gao, Linyi and Hirano, Seiichi and Okazaki, Sae and Noda, Taichi and Abudayyeh, Omar O and Gootenberg, Jonathan S and Mori, Hideto and Oura, Seiya and Holmes, Benjamin and Tanaka, Mamoru and Seki, Motoaki and Hirano, Hisato and Aburatani, Hiroyuki and Ishitani, Ryuichiro and Ikawa, Masahito and Yachie, Nozomu and Zhang, Feng and Nureki, Osamu
Science, ISSN 0036-8075, 09/2018, Volume 361, Issue 6408, pp. 1259 - 1262
The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genomeediting tool. However, the widely used Streptococcus pyogenes Cas9 enzyme... 
DNA CLEAVAGE | VARIANTS | RECOGNITION | STRUCTURAL BASIS | MULTIDISCIPLINARY SCIENCES | CPF1 | RNA-GUIDED ENDONUCLEASE | CAS9 | ALTERED PAM SPECIFICITIES | Endonucleases - genetics | Humans | Bacterial Proteins - chemistry | Bacterial Proteins - genetics | CRISPR-Cas Systems | HEK293 Cells | Protein Engineering | Crystallography, X-Ray | CRISPR-Associated Protein 9 | Gene Editing | Endonucleases - chemistry | Genetic research | Enzymes | Nucleases | RNA | Structure | Cells | Crystals | CRISPR | Target recognition | Streptococcus | Nucleotide sequence | Activation-induced cytidine deaminase | Genomes | Nuclease | Endonuclease | Ribonucleic acid--RNA | Deoxyribonucleic acid--DNA | Crystal structure
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10. Full Text The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair
by Jasin, Maria and Haber, James E
DNA Repair, ISSN 1568-7864, 08/2016, Volume 44, pp. 6 - 16
DNA double-strand breaks (DSBs) are dangerous lesions that if not properly repaired can lead to genomic change or cell death. Organisms have developed several... 
HO endonuclease | I-SceI endonuclease | Homologous recombination | Gene editing | CRISPR-Cas9 | Double-strand break repair | Nonhomologous end-joining | Homology-directed repair | I-SCEI | ZINC-FINGER NUCLEASES | SACCHAROMYCES-CEREVISIAE | MAMMALIAN-CELLS | CHROMOSOMAL TRANSLOCATION FORMATION | DNA END RESECTION | INDUCED REPLICATION | EMBRYONIC STEM-CELLS | GENETICS & HEREDITY | RNA-GUIDED ENDONUCLEASE | TOXICOLOGY | Endonucleases - genetics | Humans | Endonucleases - metabolism | DNA Breaks, Double-Stranded | Deoxyribonucleases, Type II Site-Specific - genetics | DNA End-Joining Repair | DNA Mismatch Repair | Clustered Regularly Interspaced Short Palindromic Repeats | Endodeoxyribonucleases - metabolism | RNA-Directed DNA Polymerase - genetics | Recombinational DNA Repair | RNA-Directed DNA Polymerase - metabolism | Transcription Activator-Like Effector Nucleases - metabolism | Bacterial Proteins - genetics | DNA - metabolism | Saccharomyces cerevisiae Proteins - genetics | Transcription Activator-Like Effector Nucleases - genetics | DNA - genetics | Deoxyribonucleases, Type II Site-Specific - metabolism | Endodeoxyribonucleases - genetics | CRISPR-Cas Systems | Saccharomyces cerevisiae Proteins - metabolism | Bacterial Proteins - metabolism | Zinc Fingers - genetics | Gene Editing - methods | Democratization | Genetic research | RNA | Cell death | Genes | Biotechnology | Nucleases | Anopheles | Developmental biology | homologous recombination | nonhomologous end-joining | homology-directed repair | gene editing
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11. Full Text The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit
by Murugan, Karthik and Babu, Kesavan and Sundaresan, Ramya and Rajan, Rakhi and Sashital, Dipali G
Molecular Cell, ISSN 1097-2765, 10/2017, Volume 68, Issue 1, pp. 15 - 25
CRISPR-Cas systems defend prokaryotes against bacteriophages and mobile genetic elements and serve as the basis for revolutionary tools for genetic... 
C2c2 | genome editing | C2c1 | CRISPR-Cas | Cpf1 | RNA-guided DNA cleavage | Cas13 | Cas9 | Cas12 | RNA-guided RNA cleavage | PAM RECOGNITION | IMMUNITY | DNA CLEAVAGE | STRUCTURAL BASIS | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | R-LOOP COMPLEX | CPF1 | RNA-GUIDED ENDONUCLEASE | GENOME-WIDE SPECIFICITIES | NUCLEASES | CELL BIOLOGY | Endonucleases - genetics | Bacteriophages - growth & development | RNA, Guide - genetics | RNA, Guide - metabolism | Humans | Bacterial Proteins - chemistry | Bacterial Proteins - genetics | Models, Molecular | Endonucleases - metabolism | Bacteria - genetics | Endonucleases - chemistry | Bacteria - immunology | Endonucleases - classification | Genetic Engineering | CRISPR-Cas Systems | Clustered Regularly Interspaced Short Palindromic Repeats | Protein Domains | Bacterial Proteins - metabolism | Protein Conformation | Transcription, Genetic | Bacterial Proteins - classification | Gene Editing - methods | Genome | Bacteria - virology | Genetic engineering | Genetically modified organisms | Phylogeny | Nucleases | Transposons | RNA | Analysis | Genomics
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