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Structure (London), ISSN 0969-2126, 06/2012, Volume 20, Issue 6, pp. 967 - 976
Journal Article
Journal Article
Proceedings of the National Academy of Sciences - PNAS, ISSN 0027-8424, 6/2006, Volume 103, Issue 25, pp. 9428 - 9433
Journal Article
Protein expression and purification, ISSN 1046-5928, 2007, Volume 53, Issue 1, pp. 40 - 50
With demand increasing for the production of many different proteins for biophysical or biochemical analyses, rapid methods are needed for the cloning, expression and purification of native recombinant proteins... 
EPL | SUMO | LIC | Ligation independent cloning | Structural genomics | Subtractive purification | Expressed protein ligation | Biochemistry & Molecular Biology | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Biochemical Research Methods | Science & Technology | Cysteine Endopeptidases - chemistry | Recombinant Fusion Proteins - isolation & purification | Small Ubiquitin-Related Modifier Proteins - isolation & purification | Luciferases - metabolism | Ataxin-3 | Nuclear Proteins - isolation & purification | Saccharomyces cerevisiae - genetics | Substrate Specificity | Green Fluorescent Proteins - genetics | Recombinant Fusion Proteins - metabolism | Restriction Mapping | Nucleic Acid Amplification Techniques | Small Ubiquitin-Related Modifier Proteins - genetics | Nerve Tissue Proteins - chemistry | Cysteine Endopeptidases - metabolism | Genome, Fungal | Cloning, Molecular | Escherichia coli - metabolism | Polymerase Chain Reaction | Repressor Proteins - isolation & purification | Green Fluorescent Proteins - chemistry | Nuclear Proteins - genetics | Genes, Reporter | Repressor Proteins - metabolism | Green Fluorescent Proteins - isolation & purification | Protein Structure, Tertiary | Green Fluorescent Proteins - metabolism | Repressor Proteins - chemistry | Small Ubiquitin-Related Modifier Proteins - metabolism | Repressor Proteins - genetics | Nuclear Proteins - metabolism | Recombinant Fusion Proteins - chemistry | DNA, Fungal | Nuclear Proteins - chemistry | Nerve Tissue Proteins - genetics | Saccharomyces cerevisiae - chemistry | Nerve Tissue Proteins - metabolism | Escherichia coli - genetics | Plasmids | Cysteine Endopeptidases - genetics | Nerve Tissue Proteins - isolation & purification | Saccharomyces cerevisiae Proteins - metabolism | Chromatography, Gel | Genetic Vectors | Histidine - chemistry | Glutamic Acid - chemistry | Saccharomyces cerevisiae Proteins - chemistry | Escherichia coli | Cloning | Index Medicus | structural genomics | expressed protein ligation | subtractive purification
Journal Article
PloS one, ISSN 1932-6203, 03/2016, Volume 11, Issue 3, pp. e0150239 - e0150239
...*) allows stringent streptavidin affinity purification of proximal proteins. This socalled BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP... 
Science & Technology - Other Topics | Multidisciplinary Sciences | Science & Technology | Recombinant Fusion Proteins - isolation & purification | Humans | Trans-Activators - isolation & purification | RNA, Messenger - metabolism | Carbon-Nitrogen Ligases - isolation & purification | Recombinant Fusion Proteins - metabolism | Carbon-Nitrogen Ligases - genetics | Protein Interaction Maps | Tandem Mass Spectrometry | Cloning, Molecular | Escherichia coli - metabolism | Trans-Activators - genetics | Chromatography, Liquid | Repressor Proteins - isolation & purification | Repressor Proteins - metabolism | Cell Line | Biotinylation | Carbon-Nitrogen Ligases - metabolism | Immunoprecipitation - methods | Repressor Proteins - genetics | Escherichia coli Proteins - metabolism | Transcription Factors - genetics | Escherichia coli Proteins - isolation & purification | Nonsense Mediated mRNA Decay | Transcription Factors - metabolism | Carrier Proteins - genetics | Protein Interaction Mapping - methods | Carrier Proteins - metabolism | Escherichia coli - genetics | Carrier Proteins - isolation & purification | Escherichia coli Proteins - genetics | Recombinant Fusion Proteins - genetics | Trans-Activators - metabolism | Transcription Factors - isolation & purification | HeLa Cells | Usage | Biotin | Liquid chromatography | Research | Protein-protein interactions | Post-transcription | Phosphorylation | Immunoprecipitation | Peptides | Protein purification | mRNA turnover | Mass spectroscopy | Biochemistry | Nonsense-mediated mRNA decay | Kinases | Machinery | Chromatography | Machinery and equipment | Proteins | Chemistry | E coli | Streptavidin | Scientific imaging | Protein interaction | Mass spectrometry | Index Medicus
Journal Article