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Nature Structural & Molecular Biology, ISSN 1545-9993, 12/2009, Volume 16, Issue 12, pp. 1237 - 1243
The spliceosome is a ribonucleoprotein machine that removes introns from pre-mRNA in a two-step reaction. To investigate the catalytic steps of splicing, we... 
2ND REACTION | PRP2 | ACTIVATION | PROTEIN | BIOCHEMISTRY & MOLECULAR BIOLOGY | COMPLEXES | PRE-MESSENGER-RNA | CELL BIOLOGY | BIOPHYSICS | DEPENDENT ATPASE | INTERACTS | SLU7 | CATALYTIC STEPS | Adenosine Triphosphatases - isolation & purification | RNA Helicases - metabolism | DEAD-box RNA Helicases - isolation & purification | RNA Splicing Factors | Adenosine Triphosphatases - metabolism | Ribonucleoprotein, U5 Small Nuclear - metabolism | Ribonucleoprotein, U2 Small Nuclear - isolation & purification | Spliceosomes - ultrastructure | RNA, Fungal - metabolism | Saccharomyces cerevisiae - metabolism | Spliceosomes - metabolism | Ribonucleoprotein, U2 Small Nuclear - metabolism | Image Processing, Computer-Assisted | Microscopy, Electron - methods | Models, Biological | Saccharomyces cerevisiae Proteins - metabolism | Saccharomyces cerevisiae Proteins - isolation & purification | RNA, Fungal - isolation & purification | DEAD-box RNA Helicases - metabolism | RNA Helicases - isolation & purification | Ribonucleoprotein, U5 Small Nuclear - isolation & purification | Ribonucleoproteins, Small Nuclear - isolation & purification | Ribonucleoproteins, Small Nuclear - metabolism | Ribonucleoproteins | Physiological aspects | Genetic aspects | Research | Structure | Brewer's yeast | Health aspects | RNA splicing | Proteins | Catalysis | Mutation | Molecular structure | Molecular biology | Index Medicus
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1649, pp. 419 - 426
Identification of physiological target RNAs and protein interactors bound to RNA-binding proteins is a key prerequisite to understand the underlying mechanisms... 
Gradient fractionation | Barentsz | Immunoprecipitation | Staufen2 | Neuronal RNA granules | Differential centrifugation | Brain - metabolism | Microspheres | Ribonucleoproteins - isolation & purification | Animals | Chemical Fractionation | Centrifugation | Neurons - metabolism | Ribonucleoproteins - metabolism | Molecular Biology - methods | Index Medicus
Journal Article
Nucleic Acids Research, ISSN 0305-1048, 2/2009, Volume 37, Issue 3, pp. 891 - 902
Journal Article
Methods, ISSN 1046-2023, 04/2017, Volume 118-119, pp. 93 - 100
We describe a tandem RNA isolation procedure (TRIP) that enables purification of formed messenger ribonucleoprotein (mRNP) complexes. The procedure relies on... 
Post-transcriptional regulation | Antisense oligonucleotides | RNA-binding protein | Ribonucleoprotein complex | RNA-protein interaction | Non-coding RNA | LOCALIZATION | INTERACTING FACTORS | RIBONUCLEOPROTEIN COMPLEXES | LIVING YEAST | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | BOUND PROTEOME | APTAMER | IDENTIFICATION | TRANSCRIPTS | BINDING PROTEINS | AFFINITY PURIFICATION TECHNIQUE | RNA-Binding Proteins - genetics | RNA, Messenger - isolation & purification | Saccharomyces cerevisiae - genetics | Humans | Oligodeoxyribonucleotides - chemistry | RNA, Messenger - metabolism | Saccharomyces cerevisiae - metabolism | Microspheres | Ultraviolet Rays | Oligodeoxyribonucleotides - genetics | Base Sequence | HEK293 Cells | Ribonucleoproteins - genetics | Oligoribonucleotides, Antisense - metabolism | Binding Sites | Oligodeoxyribonucleotides - metabolism | Streptavidin - chemistry | Blotting, Western - methods | Biotinylation | Caenorhabditis elegans - metabolism | Caenorhabditis elegans - genetics | RNA, Messenger - genetics | Ribonucleoproteins - metabolism | Oligoribonucleotides, Antisense - chemistry | Animals | Oligoribonucleotides, Antisense - genetics | Protein Binding | RNA-Binding Proteins - metabolism | Antisense RNA | Genetic engineering | Messenger RNA | Protein binding | Index Medicus
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