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PLoS ONE, ISSN 1932-6203, 09/2017, Volume 12, Issue 9, p. e0184236
The ATP binding cassette (ABC) transporters Pdr11p and its paralog Aus1p are expressed under anaerobic growth conditions at the plasma membrane of the yeast... 
CELLS | CHOLESTEROL | MULTIDISCIPLINARY SCIENCES | HYDROLYSIS | NUCLEOTIDE-BINDING | ABC TRANSPORTERS | MULTIDRUG-RESISTANCE | PROTEINS | STEROL UPTAKE | P-GLYCOPROTEIN | LIPIDS | Gene Expression | Saccharomyces cerevisiae - genetics | Recombinant Proteins - chemistry | Saccharomyces cerevisiae Proteins - biosynthesis | Cell Membrane - genetics | Recombinant Proteins - genetics | Recombinant Proteins - biosynthesis | Saccharomyces cerevisiae Proteins - genetics | Vanadates - chemistry | Saccharomyces cerevisiae - chemistry | ATP-Binding Cassette Transporters - biosynthesis | ATP-Binding Cassette Transporters - isolation & purification | Cell Membrane - chemistry | Recombinant Proteins - isolation & purification | Saccharomyces cerevisiae - metabolism | ATP-Binding Cassette Transporters - chemistry | Liposomes - chemistry | ATP-Binding Cassette Transporters - genetics | Protein Domains | Saccharomyces cerevisiae Proteins - isolation & purification | Cell Membrane - metabolism | Saccharomyces cerevisiae Proteins - chemistry | Physiological aspects | Genetic aspects | Cell membranes | Research | Carrier proteins | Adenosine triphosphatase | Plasma | Baking yeast | Laboratories | Protein purification | Fluorescence | Lipids | Biochemistry | Genomes | Orthovanadate | pH effects | Proteins | Sterols | Physiology | ABC transporters | Phosphatidylserine | Binding | Growth conditions | Purification | Methionine | Anaerobic conditions | Cholesterol | Environmental science | Mutagenesis | Lysine | Green fluorescent protein | Scientific imaging | Liposomes | ATP | Mass spectrometry | Transporter
Journal Article
Nature, ISSN 0028-0836, 04/2018, Volume 556, Issue 7701, pp. 339 - 344
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2017, Volume 1567, pp. 33 - 42
The budding yeast Saccharomyces cerevisiae is an important model organism to study cellular structure and function. Due to its excellent accessibility to... 
Isolation and purification of mitochondria | Spheroplasts | Homogenization | Cellular fractionation | Yeast culture media | Saccharomyces cerevisiae - metabolism | Centrifugation, Density Gradient - methods | Mitochondria - metabolism | Mitochondria - ultrastructure | Cell Fractionation - methods
Journal Article
Nature Structural & Molecular Biology, ISSN 1545-9993, 12/2009, Volume 16, Issue 12, pp. 1237 - 1243
The spliceosome is a ribonucleoprotein machine that removes introns from pre-mRNA in a two-step reaction. To investigate the catalytic steps of splicing, we... 
2ND REACTION | PRP2 | ACTIVATION | PROTEIN | BIOCHEMISTRY & MOLECULAR BIOLOGY | COMPLEXES | PRE-MESSENGER-RNA | CELL BIOLOGY | BIOPHYSICS | DEPENDENT ATPASE | INTERACTS | SLU7 | CATALYTIC STEPS | Adenosine Triphosphatases - isolation & purification | RNA Helicases - metabolism | DEAD-box RNA Helicases - isolation & purification | RNA Splicing Factors | Adenosine Triphosphatases - metabolism | Ribonucleoprotein, U5 Small Nuclear - metabolism | Ribonucleoprotein, U2 Small Nuclear - isolation & purification | Spliceosomes - ultrastructure | RNA, Fungal - metabolism | Saccharomyces cerevisiae - metabolism | Spliceosomes - metabolism | Ribonucleoprotein, U2 Small Nuclear - metabolism | Image Processing, Computer-Assisted | Microscopy, Electron - methods | Models, Biological | Saccharomyces cerevisiae Proteins - metabolism | Saccharomyces cerevisiae Proteins - isolation & purification | RNA, Fungal - isolation & purification | DEAD-box RNA Helicases - metabolism | RNA Helicases - isolation & purification | Ribonucleoprotein, U5 Small Nuclear - isolation & purification | Ribonucleoproteins, Small Nuclear - isolation & purification | Ribonucleoproteins, Small Nuclear - metabolism | Ribonucleoproteins | Physiological aspects | Genetic aspects | Research | Structure | Brewer's yeast | Health aspects | RNA splicing | Proteins | Catalysis | Mutation | Molecular structure | Molecular biology
Journal Article
Current Biology, ISSN 0960-9822, 05/2014, Volume 24, Issue 10, pp. 1153 - 1159
Journal Article
EMBO reports, ISSN 1469-221X, 06/2006, Volume 7, Issue 6, pp. 605 - 610
Recent developments in purification strategies, together with mass spectrometry (MS)‐based proteomics, have identified numerous in vivo protein complexes and... 
tandem affinity purification | mass spectrometry | cap‐binding complex | scavenger decapping complex | exosome | Exosome | Mass spectrometry | Cap-binding complex | Scavenger decapping complex | Tandem affinity purification | cap-binding complex | BIOCHEMISTRY & MOLECULAR BIOLOGY | CELL BIOLOGY | MESSENGER-RNA DEGRADATION | YEAST | RING | PURIFICATION | FRAMEWORK | PROTEIN-PROTEIN INTERACTIONS | LINK | Fungal Proteins - chemistry | Saccharomyces cerevisiae - genetics | Protein Subunits - analysis | Proteome - chemistry | Protein Subunits - metabolism | Saccharomyces cerevisiae - metabolism | Multiprotein Complexes - metabolism | Protein Subunits - isolation & purification | Gene Deletion | Mass Spectrometry | Protein Structure, Quaternary | Isotope Labeling | Genes, Fungal | Saccharomyces cerevisiae Proteins - isolation & purification | Proteomics - methods | Fungal Proteins - isolation & purification | Dimerization | Saccharomyces cerevisiae - chemistry | Protein Interaction Mapping | Saccharomyces cerevisiae - cytology | Fungal Proteins - analysis | Multiprotein Complexes - chemistry | Chromatography, Affinity | Models, Biological | Saccharomyces cerevisiae Proteins - metabolism | Protein Binding | Protein Subunits - chemistry | Proteome - metabolism | Exoribonucleases - metabolism | Fungal Proteins - metabolism | Multiprotein Complexes - isolation & purification | Saccharomyces cerevisiae Proteins - chemistry | Proteins | Molecular biology | Spectrum analysis | Research methodology | Cells | Proteomics | Protein Subunits | Exoribonucleases | Multiprotein Complexes | Biochemistry, Molecular Biology | Proteome | Fungal Proteins | Life Sciences | Saccharomyces cerevisiae Proteins | Biomolecules | Saccharomyces cerevisiae | Scientific Report
Journal Article
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 8/2007, Volume 104, Issue 35, pp. 13936 - 13941
Eukaryotic membrane proteins are often difficult to produce in large quantities, which is a significant obstacle for further structural and biochemical... 
Proteins | Yeasts | Gels | Plasmids | Fluorescence | Cell membranes | Detergents | Solubilization | Membrane proteins | P branes | Structural genomics | GFP-based fusion technology | structural genomics | CRYSTAL-STRUCTURE | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | RECEPTOR | IDENTIFICATION | YEAST | COPPER UPTAKE | FUNCTIONAL EXPRESSION | PICHIA-PASTORIS | ENDOPLASMIC-RETICULUM | HETEROLOGOUS PROTEINS | Gene Expression Regulation, Fungal | Recombinant Proteins - metabolism | Membrane Proteins - isolation & purification | Membrane Proteins - genetics | Recombinant Proteins - genetics | Saccharomyces cerevisiae Proteins - genetics | Recombinant Fusion Proteins - metabolism | Recombinant Proteins - isolation & purification | Saccharomyces cerevisiae - metabolism | Microscopy, Confocal | Saccharomyces cerevisiae Proteins - metabolism | Saccharomyces cerevisiae Proteins - isolation & purification | Membrane Proteins - metabolism | Genes, Reporter | Evaluation | Eukaryotes | Genomics | Physiological aspects | Genetic aspects | Research | Chemical properties | Brewer's yeast | Structure | Biological Sciences | Membrane Proteins/genetics/isolation & purification/metabolism | Saccharomyces cerevisiae/metabolism | Microscopy; Confocal | Saccharomyces cerevisiae Proteins/genetics/isolation & purification/metabolism | Recombinant Fusion Proteins/metabolism | Recombinant Proteins/genetics/isolation & purification/metabolism | Gene Expression Regulation; Fungal | Genes; Reporter
Journal Article
Science, ISSN 0036-8075, 10/2008, Volume 322, Issue 5898, pp. 104 - 110
Journal Article