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EMBO reports, ISSN 1469-221X, 06/2006, Volume 7, Issue 6, pp. 605 - 610
Recent developments in purification strategies, together with mass spectrometry (MS)‐based proteomics, have identified numerous in vivo protein complexes and suggest the existence of many others... 
tandem affinity purification | mass spectrometry | cap‐binding complex | scavenger decapping complex | exosome | Exosome | Mass spectrometry | Cap-binding complex | Scavenger decapping complex | Tandem affinity purification | cap-binding complex | RNA | BIOCHEMISTRY & MOLECULAR BIOLOGY | PURIFICATION | FRAMEWORK | PROTEIN-PROTEIN INTERACTIONS | LINK | CELL BIOLOGY | Fungal Proteins - chemistry | Saccharomyces cerevisiae - genetics | Protein Subunits - analysis | Proteome - chemistry | Protein Subunits - metabolism | Saccharomyces cerevisiae - metabolism | Multiprotein Complexes - metabolism | Protein Subunits - isolation & purification | Gene Deletion | Mass Spectrometry | Protein Structure, Quaternary | Isotope Labeling | Genes, Fungal | Saccharomyces cerevisiae Proteins - isolation & purification | Proteomics - methods | Fungal Proteins - isolation & purification | Dimerization | Saccharomyces cerevisiae - chemistry | Protein Interaction Mapping | Saccharomyces cerevisiae - cytology | Fungal Proteins - analysis | Multiprotein Complexes - chemistry | Chromatography, Affinity | Models, Biological | Saccharomyces cerevisiae Proteins - metabolism | Protein Binding | Protein Subunits - chemistry | Proteome - metabolism | Exoribonucleases - metabolism | Fungal Proteins - metabolism | Multiprotein Complexes - isolation & purification | Saccharomyces cerevisiae Proteins - chemistry | Proteins | Molecular biology | Spectrum analysis | Research methodology | Cells | Proteomics | Protein Subunits | Exoribonucleases | Multiprotein Complexes | Biochemistry, Molecular Biology | Proteome | Fungal Proteins | Life Sciences | Saccharomyces cerevisiae Proteins | Biomolecules | Saccharomyces cerevisiae | Scientific Report
Journal Article
Science (American Association for the Advancement of Science), ISSN 1095-9203, 2008, Volume 322, Issue 5898, pp. 104 - 110
Journal Article
The EMBO journal, ISSN 1460-2075, 2001, Volume 20, Issue 5, pp. 1173 - 1183
In Saccharomyces cerevisiae, Cdc13 has been proposed to mediate telomerase recruitment at telomere ends... 
protein‐protein interactions | DNA damage checkpoints | Cdc13 | Saccharomyces cerevisiae | telomere length | Telomere length | Protein-protein interactions | CATALYTIC SUBUNIT | DNA-POLYMERASE-ALPHA | BIOCHEMISTRY & MOLECULAR BIOLOGY | REVERSE-TRANSCRIPTASE | BINDING-PROTEIN | EST1 | SACCHAROMYCES-CEREVISIAE | protein-protein interactions | CELL BIOLOGY | YEAST | MAINTENANCE | IN-VIVO | Chromosomal Proteins, Non-Histone - isolation & purification | Saccharomyces cerevisiae - genetics | DNA-Binding Proteins - metabolism | Saccharomyces cerevisiae - metabolism | Cyclin B - genetics | Flow Cytometry | Saccharomyces cerevisiae Proteins - isolation & purification | Telomerase - metabolism | Telomere - metabolism | Fungal Proteins - isolation & purification | Genes, Essential - genetics | Telomere - genetics | Chromosomal Proteins, Non-Histone - metabolism | Cell Cycle Proteins - metabolism | Fungal Proteins - genetics | Saccharomyces cerevisiae Proteins - genetics | DNA-Binding Proteins - genetics | DNA-Binding Proteins - isolation & purification | Mutation - genetics | Precipitin Tests | Chromosomal Proteins, Non-Histone - genetics | Saccharomyces cerevisiae - cytology | Two-Hybrid System Techniques | Phenotype | Cell Cycle | Models, Biological | Alleles | Saccharomyces cerevisiae Proteins - metabolism | Protein Binding | Saccharomyces cerevisiae - enzymology | Cyclin B - metabolism | DNA Damage | Fungal Proteins - metabolism | Life Sciences | protein–protein interactions
Journal Article
Science (American Association for the Advancement of Science), ISSN 1095-9203, 2007, Volume 316, Issue 5821, pp. 106 - 109
.... We report a previously uncharacterized class of inositol pyrophosphate synthase and find it is identical to yeast Vip1 and Asp1 proteins, regulators of actin-related protein-2/3 (ARP 2/3) complexes... 
Inositols | Yeasts | Enzymes | Cell growth | Cell walls | B lymphocytes | Reports | Diphosphates | Nuclear magnetic resonance | High temperature | Cells | DICTYOSTELIUM | ACTIN | METABOLISM | MULTIDISCIPLINARY SCIENCES | TETRAKISPHOSPHATE | PHOSPHATES | ARP2/3 COMPLEX | MESSENGER-RNA EXPORT | POLYPHOSPHATES | SACCHAROMYCES-CEREVISIAE | PYROPHOSPHATES | Recombinant Fusion Proteins - isolation & purification | Schizosaccharomyces pombe Proteins - chemistry | Phosphorylation | Schizosaccharomyces - growth & development | Temperature | Cytoskeletal Proteins - genetics | Saccharomyces cerevisiae - genetics | Humans | Phosphotransferases (Phosphate Group Acceptor) - genetics | Molecular Sequence Data | Substrate Specificity | Cytoskeletal Proteins - isolation & purification | Chromatography, High Pressure Liquid | Recombinant Fusion Proteins - metabolism | Phosphotransferases (Phosphate Group Acceptor) - chemistry | Schizosaccharomyces - genetics | Phytic Acid - metabolism | Schizosaccharomyces pombe Proteins - metabolism | Actin-Related Protein 3 - metabolism | Conserved Sequence | Cytoskeletal Proteins - metabolism | Saccharomyces cerevisiae Proteins - isolation & purification | Phosphotransferases (Phosphate Group Acceptor) - metabolism | Phosphotransferases (Phosphate Group Acceptor) - isolation & purification | Inositol Phosphates - metabolism | Protein Structure, Tertiary | Amino Acid Sequence | Catalytic Domain | Schizosaccharomyces pombe Proteins - genetics | Cytoskeletal Proteins - chemistry | Recombinant Fusion Proteins - chemistry | Saccharomyces cerevisiae Proteins - genetics | Schizosaccharomyces pombe Proteins - isolation & purification | Sequence Alignment | Animals | Saccharomyces cerevisiae Proteins - metabolism | Saccharomyces cerevisiae - enzymology | Schizosaccharomyces - enzymology | Schizosaccharomyces - cytology | Saccharomyces cerevisiae Proteins - chemistry | Inositol phosphates | Research | Proteins | Cellular | Alterations | Regulators | Kinases | Metabolism
Journal Article
Molecular cell, ISSN 1097-2765, 2016, Volume 64, Issue 6, pp. 1135 - 1143
RNA polymerase I (Pol I) is a 14-subunit enzyme that solely synthesizes pre-ribosomal RNA. Recently, the crystal structure of apo Pol I gave unprecedented... 
rDNA transcription | cryoelectron microscopy | transcription | RNA polymerase I | Pol I | elongation complex | VISUALIZATION | CONSERVATION | INHIBITION | CRYSTAL-STRUCTURE | DNA | ELONGATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | TRANSCRIPTION | RESOLUTION | ARCHITECTURE | INITIATION COMPLEX | CELL BIOLOGY | Protein Multimerization | Crystallography, X-Ray | RNA Polymerase I - metabolism | Protein Subunits - metabolism | RNA - genetics | Protein Subunits - isolation & purification | Saccharomyces cerevisiae Proteins - isolation & purification | Protein Interaction Domains and Motifs | Binding Sites | RNA Polymerase I - genetics | Protein Subunits - genetics | RNA - metabolism | Protein Structure, Tertiary | Recombinant Proteins - metabolism | Amino Acid Sequence | Protein Conformation, alpha-Helical | Gene Expression | Models, Molecular | Recombinant Proteins - chemistry | Recombinant Proteins - genetics | DNA - metabolism | RNA Polymerase I - chemistry | Saccharomyces cerevisiae Proteins - genetics | RNA - chemistry | Protein Folding | Saccharomyces cerevisiae - chemistry | DNA - genetics | DNA - chemistry | Saccharomyces cerevisiae Proteins - metabolism | Protein Binding | Saccharomyces cerevisiae - enzymology | Protein Subunits - chemistry | RNA Polymerase I - isolation & purification | Saccharomyces cerevisiae Proteins - chemistry | Enzymes | Structure | Molecular biology | Ribosomal RNA | Crystals | Genetic transcription | RNA polymerases | Short
Journal Article
Protein expression and purification, ISSN 1046-5928, 2015, Volume 116, pp. 42 - 49
•We present a set of protein expression vectors to screen for optimal fusion tags... 
Scytovirin | SUMO system | Solubility enhancement tag | TonB | Protein ligation | Fusion proteins | Ulp1 protease | SPLICING ELEMENT | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | AFFINITY TAGS | BIOCHEMICAL RESEARCH METHODS | LIGATION | IN-VITRO | MULTIDOMAIN PROTEIN | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | RECOMBINANT PROTEINS | C-TERMINAL DOMAIN | INTEIN | Cyanobacteria - genetics | Cysteine Endopeptidases - chemistry | Recombinant Fusion Proteins - isolation & purification | Small Ubiquitin-Related Modifier Proteins - isolation & purification | Saccharomyces cerevisiae - genetics | Cyanobacteria - chemistry | Bacterial Proteins - chemistry | Helicobacter pylori - genetics | Lectins - isolation & purification | Small Ubiquitin-Related Modifier Proteins - genetics | Proteolysis | Base Sequence | Cloning, Molecular - methods | Carrier Proteins - chemistry | Saccharomyces cerevisiae Proteins - isolation & purification | Lectins - chemistry | Pseudomonas - genetics | Membrane Proteins - isolation & purification | Genetic Vectors - chemistry | Helicobacter pylori - chemistry | Membrane Proteins - genetics | Bacterial Proteins - genetics | Solubility | Recombinant Fusion Proteins - chemistry | Saccharomyces cerevisiae Proteins - genetics | Escherichia coli - chemistry | Genetic Vectors - genetics | Saccharomyces cerevisiae - chemistry | Carrier Proteins - genetics | Membrane Proteins - chemistry | Cysteine Endopeptidases - isolation & purification | Escherichia coli - genetics | Small Ubiquitin-Related Modifier Proteins - chemistry | Carrier Proteins - isolation & purification | Cysteine Endopeptidases - genetics | Recombinant Fusion Proteins - genetics | Lectins - genetics | Bacterial Proteins - isolation & purification | Pseudomonas - chemistry | Saccharomyces cerevisiae Proteins - chemistry | Proteins | Antiviral agents | Sumo | Proteases | Glutathione transferase | Escherichia coli | Nuclear magnetic resonance spectroscopy | Sugars | Protein binding
Journal Article
Proceedings of the National Academy of Sciences - PNAS, ISSN 0027-8424, 8/2007, Volume 104, Issue 35, pp. 13936 - 13941
Journal Article
Molecular cell, ISSN 1097-2765, 2007, Volume 26, Issue 1, pp. 131 - 143
The ubiquitin-mediated proteolysis of cyclin E plays a central role in cell-cycle progression, and cyclin E accumulation is a common event in cancer. Cyclin E... 
CELLCYCLE | CYCLIN-E | BIOCHEMISTRY & MOLECULAR BIOLOGY | DEPENDENT UBIQUITINATION | F-BOX PROTEINS | DEGRADATION | PROTEOLYSIS | INHIBITOR | HCDC4 | SACCHAROMYCES-CEREVISIAE | CHROMOSOMAL INSTABILITY | ENDOMETRIAL CANCER | CELL BIOLOGY | Phosphorylation | S-Phase Kinase-Associated Proteins - isolation & purification | Humans | Ubiquitin - metabolism | Molecular Sequence Data | Substrate Specificity | Structure-Activity Relationship | Threonine - metabolism | Cell Cycle Proteins - chemistry | S-Phase Kinase-Associated Proteins - chemistry | Saccharomyces cerevisiae - metabolism | Transfection | Cyclin-Dependent Kinase Inhibitor Proteins | Binding Sites | Dimerization | Protein Structure, Tertiary | Amino Acid Sequence | Cell Line | Phosphopeptides - chemistry | F-Box Proteins - metabolism | F-Box Proteins - chemistry | Cell Cycle Proteins - metabolism | SKP Cullin F-Box Protein Ligases - metabolism | Saccharomyces cerevisiae Proteins - genetics | S-Phase Kinase-Associated Proteins - metabolism | Ubiquitin-Protein Ligases - chemistry | Cyclin E - chemistry | Serine - metabolism | Saccharomyces cerevisiae - chemistry | Sequence Homology, Amino Acid | Models, Biological | Saccharomyces cerevisiae Proteins - metabolism | Phosphopeptides - metabolism | Protein Binding | Saccharomyces cerevisiae - enzymology | F-Box Proteins - isolation & purification | Saccharomyces cerevisiae Proteins - chemistry | NEOPLASMS | BASIC BIOLOGICAL SCIENCES | PHOSPHORYLATION | SUBSTRATES | DIMERIZATION | national synchrotron light source | IN VIVO | PEPTIDES | IN VITRO | YEASTS | LIGASES | PHOSPHATES | CELL CYCLE
Journal Article
Nature biotechnology, ISSN 1546-1696, 2003, Volume 21, Issue 2, pp. 163 - 170
... cytometry, obviating separate subcloning, expression, and purification steps and thereby expediting the isolation of novel affinity reagents... 
B-CELLS | FRAGMENTS | PERIPLASMIC EXPRESSION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | HIGH-AFFINITY | PHAGE DISPLAY | GENE REPERTOIRE | T-CELL RECEPTOR | SELECTION | PROTEINS | DIRECTED EVOLUTION | Flow Cytometry - methods | Microchemistry - methods | Saccharomyces cerevisiae - genetics | Humans | Immunoglobulin Variable Region - metabolism | Male | Immunoglobulin Variable Region - immunology | Peptide Library | Feasibility Studies | Polymerase Chain Reaction - methods | Recombinant Proteins - isolation & purification | Saccharomyces cerevisiae - metabolism | Microspheres | Cloning, Molecular | Female | Membrane Proteins - metabolism | Gene Expression Regulation, Fungal | Recombinant Proteins - metabolism | Membrane Proteins - isolation & purification | Saccharomyces cerevisiae - immunology | Membrane Proteins - genetics | Nanotechnology - methods | Cells, Cultured | Membrane Proteins - immunology | Recombinant Proteins - genetics | Immunoglobulin Fragments - immunology | Immunoglobulin Variable Region - isolation & purification | Recombinant Proteins - immunology | Immunoglobulin Fragments - isolation & purification | Immunoglobulin Fragments - genetics | Immunoglobulin Variable Region - genetics | Saccharomyces cerevisiae - isolation & purification | ANTIBODIES | STABILITY | SACCHAROMYCES CEREVISIAE | b-cells | ANTIGENS | gene repertoire | 60 APPLIED LIFE SCIENCES | T-cell receptor | selection | AFFINITY | proteins | PURIFICATION | YEASTS | SORTING | periplasmic expression | fragments | phage display, high-affinity | directed evolution
Journal Article
PloS one, ISSN 1932-6203, 2018, Volume 13, Issue 4, p. e0196632
Journal Article
The EMBO journal, ISSN 1460-2075, 2018, Volume 37, Issue 7, p. n/a
...Introduction The ribosome performs the essential task of decoding genetic information into proteins. While structures of the mature ribosomal subunits... 
ribosome | pre‐40S ribosome | ribosome assembly | cryo‐EM | ribosome biogenesis | cryo-EM | pre-40S ribosome | SITE | BIOCHEMISTRY & MOLECULAR BIOLOGY | KINASE | TRANSLATION INITIATION | MATURATION | CLEAVAGE | CELL BIOLOGY | YEAST | FULGIDUS RIO2 | BIOGENESIS | TRANSFER-RNA | 90S | Ribosomal Proteins - chemistry | Ribosome Subunits, Small, Eukaryotic - chemistry | Saccharomyces cerevisiae - genetics | Protein-Serine-Threonine Kinases - ultrastructure | Nuclear Proteins - ultrastructure | Saccharomyces cerevisiae - ultrastructure | Ribosomal Proteins - ultrastructure | Protein Domains | Saccharomyces cerevisiae Proteins - isolation & purification | Protein Interaction Domains and Motifs | Nuclear Proteins - genetics | Ribosome Subunits, Small, Eukaryotic - genetics | RNA Folding | Saccharomyces cerevisiae Proteins - ultrastructure | Ribosomal Proteins - genetics | RNA, Ribosomal - chemistry | RNA-Binding Proteins - chemistry | Ribosome Subunits, Small, Eukaryotic - ultrastructure | Saccharomyces cerevisiae Proteins - genetics | Nuclear Proteins - chemistry | Cryoelectron Microscopy | RNA, Ribosomal - ultrastructure | RNA-Binding Proteins - ultrastructure | Ribosomal Proteins - isolation & purification | Protein Conformation | Molecular Docking Simulation | Cytoplasm | Saccharomyces cerevisiae Proteins - chemistry | Yeast | Maturation | rRNA | Ribosomes | Docking | rRNA 20S | Electron microscopy | Ribonucleic acid--RNA | Assembly | Binding sites | Structural Biology | RNA Biology | Protein Biosynthesis & Quality Control
Journal Article