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1. Full Text Human marrow mesenchymal stem cell culture: Serum-free medium allows better expansion than classical α-MEM medium
by Meuleman, Nathalie and Tondreau, Tatiana and Delforge, Alain and Dejeneffe, Marielle and Massy, Martine and Libertalis, Mark and Bron, Dominique and Lagneaux, Laurence
European Journal of Haematology, ISSN 0902-4441, 2006, Volume 76, Issue 4, pp. 309 - 316
The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10-20% fetal calf serum (FCS)... 
Serum-free medium | Cell therapy | Expansion | Mesenchymal stem cells | PROGENITORS | serum-free medium | mesenchymal stem cells | cell therapy | BONE-MARROW | GROWTH | STROMAL CELLS | HEMATOLOGY | DIFFERENTIATE | expansion | Mesenchymal Stromal Cells - cytology | Bone Marrow Cells - cytology | Organic Chemicals | Humans | Cells, Cultured | Bone Marrow Cells - physiology | Culture Media, Serum-Free | Hematopoiesis - physiology | Cell Culture Techniques - methods | Mesenchymal Stromal Cells - physiology | Index Medicus
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2. Full Text Serum‐free complete medium, an alternative medium to mimic androgen deprivation in human prostate cancer cell line models
by Fiandalo, Michael V and Wilton, John H and Mantione, Krystin M and Wrzosek, Carol and Attwood, Kristopher M and Wu, Yue and Mohler, James L
The Prostate, ISSN 0270-4137, 02/2018, Volume 78, Issue 3, pp. 213 - 221
Background Almost all men who present with advanced prostate cancer (CaP) and some men who fail therapy for clinically localized CaP are treated with androgen... 
charcoal‐stripped media | DHT | mass spectrometry | prostate cancer | androgen deprivation | charcoal-stripped media | TESTOSTERONE | RECEPTOR | FETAL BOVINE SERUM | LNCAP MODEL | ENDOCRINOLOGY & METABOLISM | UROLOGY & NEPHROLOGY | DIHYDROTESTOSTERONE | PROGRESSION | Prostatic Neoplasms - metabolism | Tandem Mass Spectrometry | Humans | Cell Line, Tumor | Culture Media, Serum-Free | Male | Androgens - metabolism | Physiological aspects | Testosterone | Liquid chromatography | Mass spectrometry | Prostate cancer | Analysis | Cell culture | Media (culture) | Media | Mass spectroscopy | Metabolism | Androgens | Cell growth | Cell lines | Prostate | Charcoal | Index Medicus | Androgen deprivation
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3. Full Text Optimized survival of hippocampal neurons in B27‐supplemented neurobasal™, a new serum‐free medium combination
by Brewer, G.J and Brewer, G.J and Torricelli, J.R and Torricelli, J.R and Evege, E.K and Evege, E.K and Price, P.J and Price, P.J
Journal of Neuroscience Research, ISSN 0360-4012, 1993, Volume 35, Issue 5, pp. 567 - 576
We have systematically optimized the concentrations of 20 components of a previously published serum-free medium (Brewer and Cotman, Brain Res 494: 65-74,... 
toxicology | differentiation | long-term survival | low-density survival | low‐density survival | long‐term survival | DEFINED MEDIUM | LONG-TERM SURVIVAL | LOW-DENSITY SURVIVAL | GROWTH | TISSUE-CULTURE | TOXICOLOGY | DIFFERENTIATION | ADVANTAGES | NEUROSCIENCES | Asparagine - pharmacology | Cell Survival - drug effects | Embryo, Mammalian | Glial Fibrillary Acidic Protein - analysis | Cells, Cultured | Rats | Culture Media, Serum-Free | Neurons - cytology | Hippocampus - cytology | Rats, Sprague-Dawley | Cell Division - drug effects | Culture Media, Conditioned | Neuroglia - cytology | Animals | Time Factors | Kinetics | Neurons - drug effects | Index Medicus
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4. Full Text Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34+ and CD34- progenitors with distinct characteristics
by Chicha, Laurie and Feki, Anis and Boni, Alessandro and Irion, Olivier and Hovatta, Outi and Jaconi, Marisa
PLoS ONE, ISSN 1932-6203, 2011, Volume 6, Issue 2, pp. e14733 - e14733
Background: Differentiation of pluripotent stem cells in vitro provides a powerful means to investigate early developmental fates, including hematopoiesis. In... 
IMMUNOGENICITY | MULTIDISCIPLINARY SCIENCES | EXPANSION | HUMAN FIBROBLASTS | GROWTH-FACTOR | ANEMIA | FLT3 LIGAND | LINES | Myeloid Cells - cytology | Embryonic Stem Cells - metabolism | Antigens, CD34 - metabolism | Induced Pluripotent Stem Cells - drug effects | Induced Pluripotent Stem Cells - physiology | Humans | Myeloid Cells - physiology | Pluripotent Stem Cells - physiology | Cells, Cultured | Cell Size | Hematopoietic Stem Cells - metabolism | Embryonic Stem Cells - physiology | Cell Lineage - physiology | Animals | Cell Differentiation - drug effects | Hematopoietic Stem Cells - cytology | Pluripotent Stem Cells - drug effects | Culture Media - pharmacology | Hematopoietic Stem Cells - physiology | Myeloid Cells - metabolism | Cell Culture Techniques | Culture Media - chemistry | Culture Media, Serum-Free - pharmacology | Induced Pluripotent Stem Cells - cytology | Cell culture | Flow cytometry | Senescence | Laboratories | Oct-4 protein | Differentiation (biology) | Erythrocytes | Embryo cells | Stem cell transplantation | Leukocytes | Kinases | Assaying | Experiments | Blood | Thrombopoietin | Stem cell factor | Red blood cells | Allografts | Immunology | Cord blood | Hematopoiesis | Rodents | Fibroblasts | Bone marrow | Vascular endothelial growth factor | CD34 antigen | Medical research | Food sources | Colonies | Anemia | Fetuses | Gynecology | Runx1 protein | Cultures | Gene expression | Erythroid cells | Cell differentiation | Embryos | Obstetrics | Hemopoiesis | Endothelium | White blood cells | Pathology | Chemotherapy | Stem cells | Animal protein | Ligands | Pluripotency | Index Medicus
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5. Full Text The role of insulin in transdifferentiated hepatocyte proliferation and function in serum‐free medium
by Gu, Ce and Li, Panpan and Liu, Wei and Zhou, Yan and Tan, Wen‐Song
Journal of Cellular and Molecular Medicine, ISSN 1582-1838, 06/2019, Volume 23, Issue 6, pp. 4165 - 4178
Transdifferentiated hepatocytes are potential seeding cells for bioartificial liver (BAL) treatment, and it is important to obtain a sufficient number of... 
insulin | proliferation | serum‐free medium | hepatocyte function | BAL | transdifferentiated hiHeps | serum-free medium | SURVIVAL | MEDICINE, RESEARCH & EXPERIMENTAL | APOPTOSIS | LIPID-METABOLISM | CELL-CYCLE PROGRESSION | P53 | CELL BIOLOGY | PROMOTES PROLIFERATION | GLUCOSE | GROWTH | EXPRESSION | Albumin | Insulin | Analysis | Liver | TOR protein | Cell proliferation | AKT1 protein | Therapeutic applications | p53 Protein | Gene expression | Metabolism | Cyclin D1 | Seeding | Western blotting | Transfection | Hepatocytes | Artificial organs | Viability | Original
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6. Full Text Accelerated and safe expansion of human mesenchymal stromal cells in animal serum‐free medium for transplantation and regenerative medicine
by Lange, Claudia and Cakiroglu, Figen and Spiess, Andrej‐Nikolai and Cappallo‐Obermann, Heike and Dierlamm, Judith and Zander, Axel R
Journal of Cellular Physiology, ISSN 0021-9541, 10/2007, Volume 213, Issue 1, pp. 18 - 26
Human bone marrow mesenchymal stromal cells (hMSC) are currently investigated for a variety of therapeutic applications. However, most expansion protocols... 
Cell Proliferation | Cell Separation | Humans | Mesenchymal Stromal Cells - metabolism | Culture Media, Serum-Free | Gene Expression Profiling | Cell Culture Techniques - methods | Mesenchymal Stromal Cells - immunology | Regeneration | Mesenchymal Stromal Cells - cytology | Phenotype | Animals | Blood Platelets - metabolism | Karyotyping | Cell Differentiation | Safety | Mesenchymal Stem Cell Transplantation | Index Medicus
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7. Full Text Serum-free corneal organ culture medium (SFM) but not conventional minimal essential organ culture medium (MEM) protects human corneal endothelial cells from apoptotic and necrotic cell death
by Jäckel, Thekla and Knels, Lilla and Valtink, Monika and Funk, Richard H W and Engelmann, Katrin
British Journal of Ophthalmology, ISSN 0007-1161, 01/2011, Volume 95, Issue 1, pp. 123 - 130
AimTo evaluate the influence of organ culture media on corneal endothelial cell survival.MethodsThe human corneal endothelial cell line HCEC-12 was cultured in... 
SURVIVAL | STORAGE | OPHTHALMOLOGY | BCL-2 | SUPRAVITAL EXPOSURE | EXPRESSION | Immunohistochemistry | Cell Line | Cell Survival - drug effects | Endothelium, Corneal - cytology | Apoptosis - drug effects | Humans | Cells, Cultured | Organ Preservation Solutions - pharmacology | Endothelium, Corneal - drug effects | Animals | Flow Cytometry | Culture Media, Serum-Free - pharmacology | Organ Culture Techniques | Culture media (Biology) | Cornea | Physiological aspects | Influence | Research | Organs, Culture of | Endothelium | Apoptosis | Flow cytometry | Transplants & implants | Antibiotics | Morphology | Metabolism | Experiments | Index Medicus
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8. Full Text Ultrastructure of bovine embryos developed from in vitro–matured and –fertilized oocytes: Comparative morphological evaluation of embryos cultured either in serum‐free medium or in serum‐supplemented medium
by Abe, Hiroyuki and Yamashita, Shoko and Itoh, Takehiro and Satoh, Takeshi and Hoshi, Hiroyoshi
Molecular Reproduction and Development, ISSN 1040-452X, 07/1999, Volume 53, Issue 3, pp. 325 - 335
The ultrastructure of bovine embryos developed from in vitro‐matured and ‐fertilized oocytes, cocultured with bovine cumulus/granulosa cells either in a... 
lipid droplet | development | morphology | embryo culture | Development | Lipid droplet | Embryo culture | Morphology | BIOCHEMISTRY & MOLECULAR BIOLOGY | DEVELOPMENTAL BIOLOGY | COCULTURE | CELL BIOLOGY | SUPPORT | IN-VITRO | REPRODUCTIVE BIOLOGY | DEFINED MEDIUM | SYSTEMS | INVITRO DEVELOPMENT | Oocytes - ultrastructure | Blastocyst - ultrastructure | Animals | Fertilization in Vitro | Zygote - ultrastructure | Cattle | Culture Media | Culture Media, Serum-Free | Female | Male | Morula - ultrastructure | Embryonic and Fetal Development | Index Medicus
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9. Full Text Proteomic Analysis of Human Dermal Fibroblast Conditioned Medium (DFCM)
by Maarof, Manira and Lokanathan, Yogeswaran and Ruszymah, Hj Idrus and Saim, Aminuddin and Chowdhury, Shiplu Roy
The Protein Journal, ISSN 1572-3887, 12/2018, Volume 37, Issue 6, pp. 589 - 607
Growth factors and extracellular matrix (ECM) proteins are involved in wound healing. Human dermal fibroblasts secrete wound-healing mediators in culture... 
Biochemistry, general | Chemistry | Wound healing | Conditioned medium | Secretory protein | Dermal fibroblast | Animal Anatomy / Morphology / Histology | Bioorganic Chemistry | Organic Chemistry | BIOCHEMISTRY & MOLECULAR BIOLOGY | SERUM-FREE MEDIUM | KERATINOCYTES | PROLIFERATION | IDENTIFICATION | SECRETED PROTEINS | CALCIUM | FIBRONECTIN | EXTRACELLULAR-MATRIX | GROWTH-FACTOR | CELL | Culture Media, Conditioned - analysis | Dermis - metabolism | Humans | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods | Fibroblasts - cytology | Proteomics - methods | Dermis - cytology | Proteome - metabolism | Fibroblasts - metabolism | Fibronectins | Proteins | Care and treatment | Tissue engineering | Analysis | Physiological aspects | Liquid chromatography | Skin | Mass spectrometry | Wounds and injuries | Enzyme-linked immunosorbent assay | Cell culture | Media (culture) | Leukocyte migration | Fibronectin | Cell adhesion & migration | Serum-free medium | Cell adhesion | Fibroblasts | Electrophoresis | Extracellular matrix | Conditioning | Bioinformatics | Growth factors | Sodium lauryl sulfate | Statistical analysis | Gel electrophoresis | Mass spectroscopy | Serum albumin | Environmental factors | Gene expression | Biological activity | Sodium dodecyl sulfate | Sodium | Ionization | Collagen | In vivo methods and tests | Cell migration | Index Medicus
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10. Full Text A novel chemically defined serum‐ and feeder‐free medium for undifferentiated growth of porcine pluripotent stem cells
by Zhang, Xue and Xue, Binghua and Li, Yan and Wei, Renyue and Yu, Zhuoran and Jin, Junxue and Zhang, Yu and Liu, Zhonghua
Journal of Cellular Physiology, ISSN 0021-9541, 09/2019, Volume 234, Issue 9, pp. 15380 - 15394
Development and improvement of in vitro culture system supporting self‐renewal and unlimited proliferation of porcine pluripotent stem cells (pPSCs) is an... 
KOFL | serum‐free | pluripotent stem cells | feeder‐free | pig | feeder-free | serum-free | Swine | Phosphatases | Stem cells | Cell proliferation | Alkaline phosphatase | Cell culture | Oct-4 protein | Karyotypes | Fluorescence | Stem cell transplantation | Embryos | Optimization | Organic chemistry | Morphology | Green fluorescent protein | Culture | Pluripotency
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11. Full Text Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products
by Li, Yan and Powell, Sandra and Brunette, Elisa and Lebkowski, Jane and Mandalam, Ramkumar
Biotechnology and Bioengineering, ISSN 0006-3592, 09/2005, Volume 91, Issue 6, pp. 688 - 698
Human embryonic stem cells (hESCs) can serve as an unlimited cell source for cellular transplantation and tissue engineering due to their prolonged... 
Defined serum-free medium | Human embryonic stem cells | Expansion | Animal-free | PROGENITOR CELLS | animal-free | SELF-RENEWAL | defined serum-free medium | HUMAN BLASTOCYSTS | expansion | IN-VITRO | FEEDER LAYERS | CULTURE-SYSTEM | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GROWTH | HUMAN TELOMERASE | DIFFERENTIATION | human embryonic stem cells | HEMATOPOIETIC STEM | Growth Substances - administration & dosage | Culture Media, Serum-Free - chemistry | Humans | Cells, Cultured | Culture Media, Conditioned - pharmacology | Fibroblast Growth Factors - pharmacology | Stem Cells - cytology | Recombinant Proteins - pharmacology | Cell Culture Techniques - methods | Dose-Response Relationship, Drug | Animals | Laminin | Cell Differentiation - drug effects | Embryo, Mammalian - cytology | Growth Substances - pharmacology | Stem Cells - drug effects | Culture Media, Serum-Free - pharmacology | Embryos | Stem cells | Bioengineering | Feeders | Conditioning | Gene expression | Culture | Recombinant | Index Medicus
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12. Full Text Optimization of production of vesicular stomatitis virus (VSV) in suspension serum-free culture medium at high cell density
by Elahi, Seyyed Mehdy and Shen, Chun Fang and Gilbert, Rénald
Journal of Biotechnology, ISSN 0168-1656, 01/2019, Volume 289, pp. 144 - 149
During the last decade, oncolytic viruses such as vesicular stomatitis virus (VSV) have gained tremendous popularity as efficient vaccines for infectious... 
Oncolytic viruses | Vesicular stomatitis virus (VSV) | Fed-batch process | Suspension culture | Stable cell line | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | VACCINE | INFECTION | CANDIDATE | VECTORS | Cell Line | Green Fluorescent Proteins | Animals | Virus Replication | Cell Count | Humans | Cercopithecus aethiops | Culture Media, Serum-Free | Vesiculovirus - physiology | Fluorescence | Stomatitis | Specific gravity | Health aspects | Communicable diseases | Index Medicus
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13. Full Text Heparin Promotes the Growth of Human Embryonic Stem Cells in a Defined Serum-Free Medium
by Miho K. Furue and Jie Na and Jamie P. Jackson and Tetsuji Okamoto and Mark Jones and Duncan Baker and Ryu-Ichiro Hata and Harry D. Moore and J. Denry Sato and Peter W. Andrews
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 9/2008, Volume 105, Issue 36, pp. 13409 - 13414
A major limitation in developing applications for the use of human embryonic stem cells (HESCs) is our lack of knowledge of their responses to specific cues... 
Feeder cells | Antigens | Cell growth | Stem cells | Collagens | Cell lines | Cultured cells | Heparin | Embryonic stem cells | Pluripotent stem cells | Feeder-free | Defined serum-free culture | CARCINOMA-CELLS | MAINTAIN PLURIPOTENCY | MULTIDISCIPLINARY SCIENCES | MOUSE | SELF-RENEWAL | LEUKEMIA INHIBITORY FACTOR | MONOCLONAL-ANTIBODY | HUMAN BLASTOCYSTS | CULTURE | defined serum-free culture | FEEDER LAYERS | feeder-free | DIFFERENTIATION | Cell Line | Cell Proliferation | Embryonic Stem Cells - cytology | Embryonic Stem Cells - drug effects | Signal Transduction | Humans | Culture Media, Serum-Free | Fibroblast Growth Factors - pharmacology | Cell Culture Techniques - methods | Heparin - pharmacology | Cell culture | Cell research | Properties | Growth | Carbohydrates | Rodents | Index Medicus | Biological Sciences
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