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1. Full Text Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes
by Balzarotti, Francisco and Eilers, Yvan and Gwosch, Klaus C and Gynnå, Arvid H and Westphal, Volker and Stefani, Fernando D and Elf, Johan and Hell, Stefan W and Science for Life Laboratory, SciLifeLab and Teknisk-naturvetenskapliga vetenskapsområdet and Biologiska sektionen and Uppsala universitet and Institutionen för cell- och molekylärbiologi and Molekylär systembiologi
Science, ISSN 0036-8075, 02/2017, Volume 355, Issue 6325, pp. 606 - 612
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX... 
DISTANCE MEASUREMENTS | LOCALIZATION ANALYSIS | STIMULATED-EMISSION | FLUOROPHORES | PROTEIN | DNA ORIGAMI | MULTIDISCIPLINARY SCIENCES | NANOSCOPY | DYNAMICS | MICROSCOPY | SINGLE MOLECULES | DNA - chemistry | Luminescent Proteins - analysis | Microscopy, Fluorescence - methods | Nanotechnology - methods | Photons | Ribosome Subunits, Small, Bacterial - chemistry | Single Molecule Imaging - methods | Escherichia coli - chemistry | Optical Imaging - methods | Observations | Methods | Imaging systems | Proteins | Ribosomal subunits | Diffraction | Microscopy | E coli | Macromolecules | Fluorophores | Fluorescence | Stochasticity | Localization | Emissions | Tracking | Emitters | Excitation | Position (location) | Biological Sciences | Biofysik | Naturvetenskap | Natural Sciences | Biophysics | Biologiska vetenskaper
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2. Full Text Bright photoactivatable fluorophores for single-molecule imaging
by Grimm, Jonathan B and English, Brian P and Choi, Heejun and Muthusamy, Anand K and Mehl, Brian P and Dong, Peng and Brown, Timothy A and Lippincott-Schwartz, Jennifer and Liu, Zhe and Lionnet, Timothée and Lavis, Luke D
Nature Methods, ISSN 1548-7091, 12/2016, Volume 13, Issue 12, pp. 985 - 988
Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable... 
CELLS | LOCALIZATION MICROSCOPY | CHEMICAL BIOLOGY | SUPERRESOLUTION MICROSCOPY | IN-VIVO | RHODAMINE | BIOCHEMICAL RESEARCH METHODS | FLUORESCENT PROTEINS | PHOTOLYSIS | SOFTWARE | GENERAL-METHOD | Fluorescent Dyes - chemical synthesis | Humans | Cell Membrane Permeability | Fluorescent Dyes - metabolism | Small Molecule Libraries - metabolism | Fluorescent Dyes - radiation effects | Recombinant Fusion Proteins - metabolism | Photochemical Processes | Photochemistry - methods | Light | Molecular Structure | Cell Culture Techniques | Fluorescent Dyes - chemistry | Small Molecule Libraries - chemical synthesis | Small Molecule Libraries - radiation effects | Spectrometry, Fluorescence | Single Molecule Imaging - methods | Spectrophotometry, Ultraviolet | Small Molecule Libraries - chemistry | Animals | Staining and Labeling | Cell Line, Tumor | Ligands | Mice | Embryonic Stem Cells | COS Cells | Microscopy, Fluorescence | Diagnosis, Fluoroscopic | Molecular diagnostic techniques | Research | Molecules | Medical imaging | Dyes | Microscopy
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3. Full Text Imaging proteins at the single-molecule level
by Longchamp, Jean-Nicolas and Rauschenbach, Stephan and Abb, Sabine and Escher, Conrad and Latychevskaia, Tatiana and Kern, Klaus and Fink, Hans-Werner
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 02/2017, Volume 114, Issue 7, pp. 1474 - 1479
Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and... 
Preparative mass spectrometry | Single protein imaging | Microscopy | Low-energy electron holography | Structural biology | low-energy electron holography | NATIVE MASS-SPECTROMETRY | single protein imaging | preparative mass spectrometry | MULTIDISCIPLINARY SCIENCES | CHARGE RETENTION | TRANSMISSION ELECTRON-MICROSCOPY | CYTOCHROME-C | X-RAY LASER | LOW-ENERGY ELECTRONS | POINT-SOURCE | COHERENT DIFFRACTION | ION-BEAM DEPOSITION | microscopy | structural biology | Serum Albumin, Bovine - ultrastructure | Single Molecule Imaging - methods | Static Electricity | Hemoglobins - ultrastructure | Single Molecule Imaging - instrumentation | Animals | Vacuum | Cattle | Cytochromes c - ultrastructure | Spectrometry, Mass, Electrospray Ionization - methods | Graphite | Holography - methods | Proteins - ultrastructure | Electrons | Holography - instrumentation | Biological Sciences | Physical Sciences
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4. Full Text Ultra-High Resolution 3D Imaging of Whole Cells
by Huang, Fang and Sirinakis, George and Allgeyer, Edward S and Schroeder, Lena K and Duim, Whitney C and Kromann, Emil B and Phan, Thomy and Rivera-Molina, Felix E and Myers, Jordan R and Irnov, Irnov and Lessard, Mark and Zhang, Yongdeng and Handel, Mary Ann and Jacobs-Wagner, Christine and Lusk, C. Patrick and Rothman, James E and Toomre, Derek and Booth, Martin J and Bewersdorf, Joerg
Cell, ISSN 0092-8674, 08/2016, Volume 166, Issue 4, pp. 1028 - 1040
Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior... 
FLUORESCENCE MICROSCOPY | SUPERRESOLUTION MICROSCOPY | DIFFRACTION-LIMIT | SINGLE-MOLECULE LOCALIZATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | DEPENDENT ABERRATIONS | CILIARY TRANSITION ZONE | SYNAPTONEMAL COMPLEX | SAMPLE DRIFT CORRECTION | LIVE CELLS | OPTICAL NANOSCOPY | CELL BIOLOGY | COP-Coated Vesicles - ultrastructure | Cytological Techniques - methods | Male | Single Molecule Imaging - methods | Microscopy, Fluorescence - instrumentation | Synaptonemal Complex - ultrastructure | Cytological Techniques - instrumentation | Single Molecule Imaging - instrumentation | Animals | Bacteriophages - ultrastructure | Microscopy, Fluorescence - methods | Spermatocytes - ultrastructure | Mice | Golgi Apparatus - ultrastructure | Fluorescence | Medical colleges | Developmental biology | Cells | Biomedical engineering | Information management | Resource
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5. Full Text Fluorescent Biosensors Based on Single-Molecule Counting
by Ma, Fei and Li, Ying and Tang, Bo and Zhang, Chun-Yang
Accounts of Chemical Research, ISSN 0001-4842, 09/2016, Volume 49, Issue 9, pp. 1722 - 1730
Biosensors for highly sensitive, selective, and rapid quantification of specific biomolecules make great contributions to biomedical research, especially... 
MULTIPLEX DETECTION | CANCER-CELLS | ISOTHERMAL AMPLIFICATION | PROTEIN | ASSAY | HISTONE-MODIFYING ENZYMES | DOT-BASED NANOSENSOR | RESONANCE ENERGY-TRANSFER | SENSITIVE DETECTION | CHEMISTRY, MULTIDISCIPLINARY | MICRORNA | Fluorescent Dyes - chemistry | RNA - analysis | Humans | Fluorescence | Proteins - analysis | Single Molecule Imaging - methods | Protein Modification, Translational | Enzymes - analysis | Quantum Dots - chemistry | DNA - analysis | Biosensing Techniques - methods | Fluorescence Resonance Energy Transfer - methods | Proteins - chemistry
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6. Full Text Improved maize reference genome with single-molecule technologies
by Jiao, Yinping and Peluso, Paul and Shi, Jinghua and Liang, Tiffany and Stitzer, Michelle C and Wang, Bo and Campbell, Michael S and Stein, Joshua C and Wei, Xuehong and Chin, Chen-Shan and Guill, Katherine and Regulski, Michael and Kumari, Sunita and Olson, Andrew and Gent, Jonathan and Schneider, Kevin L and Wolfgruber, Thomas K and May, Michael R and Springer, Nathan M and Antoniou, Eric and McCombie, W. Richard and Presting, Gernot G and McMullen, Michael and Ross-Ibarra, Jeffrey and Dawe, R. Kelly and Hastie, Alex and Rank, David R and Ware, Doreen
Nature, ISSN 0028-0836, 06/2017, Volume 546, Issue 7659, pp. 524 - 527
Complete and accurate reference genomes and annotations provide fundamental tools for characterization of genetic and functional variation(1). These resources... 
POPULATION | READ ALIGNMENT | PLANT GENOMES | ANNOTATIONS | MULTIDISCIPLINARY SCIENCES | COMPLEXITY | TRANSPOSABLE ELEMENTS | QUALITY-CONTROL | MAKER-P | DIFFERENTIATION | LTR RETROTRANSPOSONS | Zea mays - genetics | Genome, Plant - genetics | Molecular Sequence Annotation | RNA, Messenger - genetics | RNA, Messenger - analysis | Phylogeny | Single Molecule Imaging - methods | DNA, Intergenic - genetics | Reference Standards | Centromere - genetics | Genes, Plant - genetics | DNA Transposable Elements - genetics | Contig Mapping | Crops, Agricultural - genetics | Sorghum - genetics | Chromosomes, Plant - genetics | High-Throughput Nucleotide Sequencing - methods | Optics and Photonics | Corn | Genetic aspects | Gene expression | Observations
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7. Full Text Single-Molecule Imaging of GPCR Interactions
by Calebiro, Davide and Sungkaworn, Titiwat
Trends in Pharmacological Sciences, ISSN 0165-6147, 02/2018, Volume 39, Issue 2, pp. 109 - 122
G protein-coupled receptors (GPCRs) constitute the largest family of membrane receptors and are of great interest as pharmacological targets. Although the... 
dimerization | GPCR | nanodomains | single-molecule microscopy | labeling | PROTEIN-COUPLED RECEPTOR | SUPERRESOLUTION MICROSCOPY | CRYSTAL-STRUCTURE | RESONANCE ENERGY-TRANSFER | PHARMACOLOGY & PHARMACY | METABOTROPIC GLUTAMATE RECEPTORS | TERNARY COMPLEX MODEL | CONFORMATIONAL DYNAMICS | CRYO-EM STRUCTURE | BETA-ADRENERGIC RECEPTOR | LIVING CELLS | Animals | Receptors, G-Protein-Coupled - metabolism | Signal Transduction | Humans | Protein Binding | Single Molecule Imaging - methods | Receptors, G-Protein-Coupled - chemistry
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8. Full Text Quantitative mass imaging of single biological macromolecules
by Young, Gavin and Hundt, Nikolas and Cole, Daniel and Fineberg, Adam and Andrecka, Joanna and Tyler, Andrew and Olerinyova, Anna and Ansari, Ayla and Marklund, Erik G and Collier, Miranda P and Chandler, Shane A and Tkachenko, Olga and Allen, Joel and Crispin, Max and Billington, Neil and Takagi, Yasuharu and Sellers, James R and Eichmann, Cédric and Selenko, Philipp and Frey, Lukas and Riek, Roland and Galpin, Martin R and Struwe, Weston B and Benesch, Justin L. P and Kukura, Philipp and Teknisk-naturvetenskapliga vetenskapsområdet and Uppsala universitet and Institutionen för kemi - BMC and Biokemi and Kemiska sektionen
Science, ISSN 0036-8075, 04/2018, Volume 360, Issue 6387, pp. 423 - 427
The cellular processes underpinning life are orchestrated by proteins and their interactions. The associated structural and dynamic heterogeneity, despite... 
LABEL-FREE | PROTEIN | ACTIN | SPECTROSCOPY | MOLECULE | MULTIDISCIPLINARY SCIENCES | HIGH-RESOLUTION | TRACKING | MECHANISMS | MICROSCOPY | AGGREGATION | Interferometry - methods | Humans | Actins - chemistry | Polymerization | Spatio-Temporal Analysis | Amyloidogenic Proteins - chemistry | Proteins - chemistry | Single Molecule Imaging - methods | Microscopy, Interference - methods | Mass Spectrometry - methods | Protein Aggregation, Pathological | Measurement | Physiological aspects | Mass (Physics) | Macromolecules | Microscope and microscopy | Methods | Amyloidogenesis | Light scattering | Glycoprotein | Molecular dynamics | Crosslinking | Lipids | Mass spectroscopy | Proteins | Microscopy | Actin | Weight reduction | Biomolecules | Kinetics | Protein interaction | Mass spectrometry | Sugars | Interferometry | Biological Sciences | Biofysik | Fysikalisk kemi | Biophysics | Chemistry with specialization in Biophysics | Chemical Sciences | Analytical Chemistry | Naturvetenskap | Kemi | Physical Chemistry | Biologiska vetenskaper | Natural Sciences | Analytisk kemi | Kemi med inriktning mot biofysik
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9. Full Text Real-time imaging of DNA loop extrusion by condensin
by Ganji, Mahipal and Shaltiel, Indra A and Bisht, Shveta and Kim, Eugene and Kalichava, Ana and Haering, Christian H and Dekker, Cees
Science, ISSN 0036-8075, 2018, Volume 360, Issue 6384, pp. 102 - 105
It has been hypothesized that SMC protein complexes such as condensin and cohesin spatially organize chromosomes by extruding DNA into large loops. We directly... 
ORGANIZATION | CHROMOSOME | COMPACTION | MECHANISM | SINGLE-MOLECULE | MULTIDISCIPLINARY SCIENCES | SMC COMPLEXES | Hydrolysis | DNA - chemistry | Multiprotein Complexes - chemistry | Time Factors | Adenosine Triphosphatases - chemistry | Single Molecule Imaging - methods | Adenosine Triphosphate - chemistry | Nucleic Acid Conformation | DNA-Binding Proteins - chemistry | Saccharomyces cerevisiae Proteins - chemistry | Cellular proteins | DNA | Imaging systems | Genetic research | Genetic aspects | Research | Methods | Yeast | Energy use | Base pairs | Genomes | Reels | Real time | Condensin | Anchors | Extrusion rate | Imaging | Extrusion | Cohesin | Chromosomes | ATP | Adenosine triphosphate | Deoxyribonucleic acid--DNA
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10. Full Text The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells
by Horvathova, Ivana and Voigt, Franka and Kotrys, Anna V and Zhan, Yinxiu and Artus-Revel, Caroline G and Eglinger, Jan and Stadler, Michael B and Giorgetti, Luca and Chao, Jeffrey A
Molecular Cell, ISSN 1097-2765, 11/2017, Volume 68, Issue 3, pp. 615 - 625.e9
RNA degradation plays a fundamental role in regulating gene expression. In order to characterize the spatiotemporal dynamics of RNA turnover in single cells,... 
P-bodies | RNAi | single-molecule | Xrn1 | live-cell imaging | fluorescence microscopy | mRNA decay | DEADENYLATION | DECAY | DECAPPING ENZYME | LIVE-CELL | BIOCHEMISTRY & MOLECULAR BIOLOGY | GENE-EXPRESSION | DEGRADATION | BODY FORMATION | PROTEINS | STRESS | TRANSLATION DYNAMICS | CELL BIOLOGY | Argonaute Proteins - metabolism | Argonaute Proteins - genetics | Humans | RNA, Messenger - genetics | Gene Expression Regulation | Microscopy, Video | Single Molecule Imaging - methods | RNA Stability | RNA, Messenger - metabolism | Transfection | Models, Genetic | HeLa Cells | Kinetics | Genes, Reporter | Microscopy, Fluorescence | Gene expression | Messenger RNA | Fluorescence | Detectors | Index Medicus
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11. Full Text Single-Molecule Imaging Reveals a Collapsed Conformational State for DNA-Bound Cohesin
by Stigler, Johannes and Çamdere, Gamze Ö and Koshland, Douglas E and Greene, Eric C
Cell Reports, ISSN 2211-1247, 05/2016, Volume 15, Issue 5, pp. 988 - 998
Cohesin is essential for the hierarchical organization of the eukaryotic genome and plays key roles in many aspects of chromosome biology. The conformation of... 
Schizosaccharomyces pombe Proteins - chemistry | Chromosomal Proteins, Non-Histone - metabolism | Cell Cycle Proteins - metabolism | Nucleosomes - metabolism | DNA - metabolism | Single Molecule Imaging - methods | Protein Transport | Cell Cycle Proteins - chemistry | Schizosaccharomyces - metabolism | Models, Biological | Base Sequence | AT Rich Sequence - genetics | Schizosaccharomyces pombe Proteins - metabolism | Protein Binding | Molecular Motor Proteins - metabolism | Protein Conformation | Porosity | Diffusion | Chromosomal Proteins, Non-Histone - chemistry
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12. Full Text Intranucleus Single-Molecule Imaging in Living Cells
by Shao, Shipeng and Xue, Boxin and Sun, Yujie
Biophysical Journal, ISSN 0006-3495, 07/2018, Volume 115, Issue 2, pp. 181 - 189
Many critical processes occurring in mammalian cells are stochastic and can be directly observed at the single-molecule level within their physiological... 
RNA-POLYMERASE-II | TRANSCRIPTION FACTOR-BINDING | BIOPHYSICS | MESSENGER-RNA | LIVE-CELL | EMBRYONIC STEM-CELLS | DYNAMICS | MICROSCOPY | MAMMALIAN-CELLS | NUCLEOCYTOPLASMIC TRANSPORT | GENERAL-METHOD | Animals | Cell Nucleus - metabolism | Single Molecule Imaging - methods | Cell Survival | Humans | Cells | Genetic transcription | Biophysical s
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