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1991, Practical approach series., ISBN 9780199631414, xx, 257
Book
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 6/2010, Volume 107, Issue 26, pp. 12034 - 12039
Site-directed mutagenesis in higher plants remains a significant technical challenge for basic research and molecular breeding. Here, we demonstrate... 
Polymerase chain reaction | Mutagenesis | DNA | Genomics | Site directed mutagenesis | Genomes | Plants | Genetic mutation | Zinc | Seedlings | Nonhomologous end joining | Ku80 | Mutation | dsDNA breaks | SEQUENCES | MULTIDISCIPLINARY SCIENCES | DOUBLE-STRAND BREAKS | RESTRICTION ENZYMES | PLANT HORMONE ABA | CLEAVAGE | SUGAR | mutation | REPAIR | GENE | nonhomologous end joining | HOMOLOGOUS RECOMBINATION | RICE | Recombinant Proteins - metabolism | Amino Acid Sequence | Promoter Regions, Genetic | Arabidopsis Proteins - genetics | Mutagenesis, Site-Directed | Genes, Plant | Molecular Sequence Data | Recombinant Proteins - genetics | DNA Repair - genetics | Transcription Factors - genetics | DNA, Plant - metabolism | DNA Breaks, Double-Stranded | Deoxyribonucleases - metabolism | Arabidopsis - metabolism | Arabidopsis - genetics | Arabidopsis Proteins - metabolism | DNA Helicases - metabolism | Deoxyribonucleases - genetics | Base Sequence | Plants, Genetically Modified | Protein Engineering | DNA Helicases - genetics | DNA, Plant - genetics | Arabidopsis thaliana | Gene mutations | Plant genetics | Genetic aspects | Zinc finger proteins | Research | Properties | Identification and classification | Proteins | Flowers & plants | Gene expression | DNA repair | Cells | Index Medicus | Data processing | ABI4 gene | Glucose | Gene deletion | Promoters | Homozygotes | Plant breeding | Somatic cells | Site-directed mutagenesis | Point mutation | Nuclease | Biological Sciences
Journal Article
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, ISSN 1422-0067, 02/2019, Volume 20, Issue 4, p. 916
Journal Article
MOLECULES, ISSN 1420-3049, 01/2019, Volume 24, Issue 1
The -glycoside hydrolases (LXYL-P1-1 and LXYL-P1-2) from Lentinula edodes (strain M95.33) can specifically hydrolyze 7--xylosyl-10-deacetyltaxol (XDT) to form... 
site-directed mutagenesis | XYLANASE | glucosidase | xylosidase | STABILITY | STRATEGY | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, MULTIDISCIPLINARY | BIOCATALYSIS | molecular docking | EVOLUTION | 7-BETA-XYLOSYL-10-DEACETYLTAXOL | GEOBACILLUS-STEAROTHERMOPHILUS | BACILLUS-SUBTILIS | TAXOL | catalytic efficiency
Journal Article
Journal of Agricultural and Food Chemistry, ISSN 0021-8561, 05/2019, Volume 67, Issue 19, pp. 5587 - 5595
Maltooligosyltrehalose synthase (MTSase) is a key enzyme in trehalose production. MTSase from Arthrobacter ramosus has poor thermostability, limiting its... 
site-directed mutagenesis | Arthrobacter ramosus | maltooligosyltrehalose synthase | thermostability | directed evolution | TREHALOSE | STABILITY | FOOD SCIENCE & TECHNOLOGY | MODEL | ENZYME | METABOLISM | AGRICULTURE, MULTIDISCIPLINARY | MUTATIONS | CHEMISTRY, APPLIED | Index Medicus
Journal Article
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2011, Volume 700, pp. 107 - 124
Journal Article
Journal Article
by Mei, XJ and Li, MS and Yang, Y and Liu, M and Mao, HY and Zhang, ML and Cao, MJ and Liu, GM
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, ISSN 0021-8561, 05/2019, Volume 67, Issue 17, pp. 4958 - 4966
The mud crab (Scylla paramamosain) is widely consumed but can cause a severe food allergic reaction. To reduce allergenicity to arginine kinase (AK),... 
site-directed mutagenesis | DIAGNOSIS | DESIGN | RECOGNITION | CRYSTAL-STRUCTURE | FOOD ALLERGY | FOOD SCIENCE & TECHNOLOGY | arginine kinase | SHRIMP ALLERGEN | peptide aptamers | PATHOGENESIS | EPITOPE | reducing allergenicity | Scylla paramamosain | AGRICULTURE, MULTIDISCIPLINARY | CHEMISTRY, APPLIED | SENSE | EPIDEMIOLOGY | Index Medicus
Journal Article
Biochemical Journal, ISSN 0264-6021, 03/2015, Volume 466, Issue 2, pp. 253 - 262
Dye-decolorizing peroxidase (DyP) of Auricularia auriculajudae has been expressed in Escherichia coli as a representative of a new DyP family, and subjected to... 
QM/MM | Site-directed mutagenesis | EPR spectroscopy | Molecular docking | Catalytic protein radical | Dye-decolorizing peroxidase | site-directed mutagenesis | LIGNIN PEROXIDASE | dye-decolorizing peroxidase | BIOCHEMISTRY & MOLECULAR BIOLOGY | CRYSTAL-STRUCTURES | RHODOCOCCUS-JOSTII RHA1 | PLEUROTUS-ERYNGII | catalytic protein radical | TYROSINE RADICALS | SUBSTRATE-INTERACTION-SITES | molecular docking | CANDIDUM DEC 1 | QM/MM METHODS | VERSATILE-PEROXIDASE | HETEROLOGOUS EXPRESSION | Fungal Proteins - chemistry | Hemeproteins - genetics | Tryptophan - chemistry | Basidiomycota - enzymology | Peroxidases - chemistry | Hemeproteins - chemistry | Surface Properties | Peroxidases - metabolism | Binding Sites | Tyrosine - chemistry | Recombinant Proteins - metabolism | Biocatalysis | Mutagenesis, Site-Directed | Oxidation-Reduction | Free Radicals - chemistry | Hemeproteins - metabolism | Models, Molecular | Recombinant Proteins - chemistry | Mutant Proteins - metabolism | Fungal Proteins - genetics | Molecular Dynamics Simulation | Mutant Proteins - chemistry | Coloring Agents - metabolism | Protein Conformation | Molecular Docking Simulation | Peroxidases - genetics | Amino Acid Substitution | Coloring Agents - chemistry | Fungal Proteins - metabolism | Index Medicus | TNM, tetranitromethane | WT, wild-type | RB5, Reactive Black 5 | NBS, N-bromosuccinimide | QM, quantum mechanics | hfcc, hyperfine coupling constant | VP, versatile peroxidase | MM, molecular mechanics | PELE, Protein Energy Landscape Exploration | ABTS, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) | LRET, long-range electron transfer | LiP, lignin peroxidase | DyP, dye-decolorizing peroxidase | VA, veratryl alcohol | RB19, Reactive Blue 19 | DMP, 2,6-dimethoxyphenol
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 08/2013, Volume 2013, Issue 8, pp. 738 - 742
Unlike traditional site-directed mutagenesis, this protocol requires only a single PCR step using full plasmid amplification to generate point mutants. The... 
Point Mutation | Promoter Regions, Genetic | Mutagenesis, Site-Directed - methods | Plasmids | Open Reading Frames | Polymerase Chain Reaction - methods
Journal Article
Journal of Biotechnology, ISSN 0168-1656, 09/2019, Volume 303, pp. 72 - 79
Carboxylic acid reductases (CARs) catalyze ATP- and NADPH-dependent reduction of carboxylic acids to corresponding aldehydes. Although successful applications... 
Protein stability | Carboxylic acid reductase | Site-directed mutagenesis | Mycobacterium abscessus | Succinic acid reduction | DOMAIN | PROTEIN-PRODUCTION | ESCHERICHIA-COLI | IDENTIFICATION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | BIOSYNTHESIS | ENZYMES | ARYL-ALDEHYDE OXIDOREDUCTASE | STRAIN | Index Medicus
Journal Article