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science & technology (7) 7
sortase-mediated ligation (7) 7
aminoacyltransferases - metabolism (6) 6
life sciences & biomedicine (6) 6
bacterial proteins - metabolism (5) 5
cysteine endopeptidases - metabolism (5) 5
aminoacyltransferases - genetics (4) 4
bacterial proteins - genetics (4) 4
biochemistry & molecular biology (4) 4
cysteine endopeptidases - genetics (4) 4
enzymes (4) 4
physical sciences (4) 4
amino acid sequence (3) 3
aminoacyltransferases - chemistry (3) 3
bacterial proteins - chemistry (3) 3
catalysis (3) 3
chemistry (3) 3
cysteine endopeptidases - chemistry (3) 3
peptides (3) 3
peptides - metabolism (3) 3
proteins - chemistry (3) 3
sortase (3) 3
animals (2) 2
applied microbiology (2) 2
bacteria (2) 2
biochemistry, general (2) 2
biological and medical sciences (2) 2
biotechnology (2) 2
biotechnology & applied microbiology (2) 2
cancer (2) 2
chemistry, medicinal (2) 2
chemistry, organic (2) 2
chemotherapy (2) 2
chromatography, high pressure liquid (2) 2
expressed protein ligation (2) 2
fluorescence (2) 2
fundamental and applied biological sciences. psychology (2) 2
general aspects (2) 2
genetic aspects (2) 2
immobilization techniques (2) 2
life sciences (2) 2
mass spectrometry (2) 2
methods. procedures. technologies (2) 2
microbiology (2) 2
molecular structure (2) 2
pharmacology & pharmacy (2) 2
physiological aspects (2) 2
protein engineering (2) 2
protein semisynthesis (2) 2
proteins (2) 2
proteins - chemical synthesis (2) 2
research (2) 2
sortase-mediated protein ligation (2) 2
sortase‐mediated ligation (2) 2
substrate specificity (2) 2
ab:antibody (1) 1
acrylamide (1) 1
adc (1) 1
adhesins, bacterial - metabolism (1) 1
adhesion (1) 1
alkylation (1) 1
analysis (1) 1
anthracycline (1) 1
antibodies, monoclonal - chemistry (1) 1
antibodies, monoclonal - immunology (1) 1
antibodies, monoclonal - isolation & purification (1) 1
antibodies, monoclonal - pharmacology (1) 1
antibody–drug conjugate (1) 1
antikörper (1) 1
auristatin (1) 1
bacteria, pathogenic (1) 1
bacterial adhesin (1) 1
bacterial adhesion (1) 1
bacteriological techniques - methods (1) 1
bacteriology (1) 1
biological activity (1) 1
biophysics (1) 1
biotechnological microorganisms (1) 1
biotechnology - methods (1) 1
block copolymers (1) 1
buildings (1) 1
catalytic domain (1) 1
cell membrane - chemistry (1) 1
cellular biology (1) 1
chemical synthesis (1) 1
chemistry techniques, synthetic (1) 1
chemistry, analytical (1) 1
chromatography, reverse-phase (1) 1
click ligation (1) 1
copper-free click chemistry (1) 1
copper‐free click chemistry (1) 1
diels–alder ligation (1) 1
directed evolution (1) 1
drug compounding (1) 1
electron microscopy (1) 1
engineering (1) 1
escherichia coli - genetics (1) 1
fc receptors (1) 1
fluorescent microspheres (1) 1
fret reporter (1) 1
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1. Full Text Sortase-mediated ligation of purely artificial building blocks
by Dai, Xiaolin and Mate, Diana M and Glebe, Ulrich and Garakani, Tayebeh Mirzaei and Körner, Andrea and Schwaneberg, Ulrich and Böker, Alexander
Polymers, ISSN 2073-4360, 02/2018, Volume 10, Issue 2, p. 151
Nanoparticles | Enzymes | Sortase-mediated ligation | Block copolymers | Polymer Science | Physical Sciences | Science & Technology | Matrix assisted | Buildings | Photon correlation spectroscopy | Electron microscopy | Silicon dioxide | Reaction products | Acrylamide | Ionization | Polyethylene glycol | Catalysis | Polymers | Chemical synthesis | Mass spectrometry | block copolymers | enzymes | nanoparticles | sortase-mediated ligation
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2. Full Text Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation
by Proft, Thomas and Proft, Thomas
Biotechnology letters, ISSN 0141-5492, 1/2010, Volume 32, Issue 1, pp. 1 - 10
Life Sciences | Biochemistry, general | Neoglycoconjugates | Biotechnology | Microbiology | Sortase-mediated ligation | Applied Microbiology | Protein circularisation | Protein conjugation | Protein immobilisation | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biological and medical sciences | General aspects | Immobilization techniques | Methods. Procedures. Technologies | Cysteine Endopeptidases - chemistry | Aminoacyltransferases - chemistry | Glycoconjugates - metabolism | Staphylococcus aureus - enzymology | Peptides - chemistry | Aminoacyltransferases - genetics | Bacterial Proteins - chemistry | Bacterial Proteins - genetics | Glycoproteins - metabolism | Glycopeptides - metabolism | Biotechnology - methods | Cysteine Endopeptidases - metabolism | Peptides - metabolism | Proteins - metabolism | Models, Biological | Cysteine Endopeptidases - genetics | Aminoacyltransferases - metabolism | Bacterial Proteins - metabolism | Proteins - chemistry | Post-translational modification | Usage | Physiological aspects | Genetic aspects | Biotechnological microorganisms | Research | Microbial biotechnology | Peptidyl transferases | Proteins | Gram-positive bacteria | Peptides | Index Medicus
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3. Full Text Peptibodies: An elegant solution for a long‐standing problem
by Cavaco, Marco and Castanho, Miguel A. R. B and Neves, Vera
Peptide science (Hoboken, N.J.), ISSN 2475-8817, 01/2018, Volume 110, Issue 1, pp. e23095 - n/a
sortase‐mediated protein ligation | peptibody | peptide‐Fc fusion protein | streamlined‐expressed protein ligation | copper‐free click chemistry | copper-free click chemistry | peptide-Fc fusion protein | streamlined-expressed protein ligation | sortase-mediated protein ligation | Biochemistry & Molecular Biology | Biophysics | Life Sciences & Biomedicine | Science & Technology | Neonates | Fc receptors | Peptides | Immunoglobulin G | Amino acid sequence | Genetic engineering | Recombinant DNA | Fusion protein | Biological activity
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4. Full Text The use of sortase-mediated ligation for the immobilisation of bacterial adhesins onto fluorescence-labelled microspheres: a novel approach to analyse bacterial adhesion to host cells
by Wu, Si and Proft, Thomas
Biotechnology letters, ISSN 0141-5492, 11/2010, Volume 32, Issue 11, pp. 1713 - 1718
Life Sciences | Biochemistry, general | Fluorescent microspheres | Biotechnology | Microbiology | Sortase-mediated ligation | Applied Microbiology | Bacterial adhesin | Host cell binding | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biological and medical sciences | General aspects | Immobilization techniques | Methods. Procedures. Technologies | Bacterial Adhesion | Adhesins, Bacterial - metabolism | Microspheres | Gram-Positive Bacteria - physiology | Bacteriological Techniques - methods | Fluorescence | Protein Binding | Aminoacyltransferases - metabolism | Bacteria, Pathogenic | Physiological aspects | Bacteria | Genetic aspects | Research | Adhesion | Pathology | Enzymes | Cellular biology | Bacteriology | Index Medicus
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5. Full Text Engineering sortase A by screening a second‐generation library using phage display
by Schmohl, Lena and Bierlmeier, Jan and Gerth, Fabian and Freund, Christian and Schwarzer, Dirk
Journal of peptide science, ISSN 1075-2617, 07/2017, Volume 23, Issue 7-8, pp. 631 - 635
sortase‐mediated ligation | protein engineering | sortase A | phage display | directed evolution | sortase-mediated ligation | Cysteine Endopeptidases - metabolism | Peptides - metabolism | Protein Engineering - methods | Cysteine Endopeptidases - genetics | Aminoacyltransferases - genetics | Bacterial Proteins - genetics | Peptides - genetics | Substrate Specificity | Aminoacyltransferases - metabolism | Bacterial Proteins - metabolism | Peptide Library | Mutation - genetics | Proteins | Chemotherapy | Cancer | Engineering | Phage display | Directed evolution | Phages | Libraries | Selectivity | Sortase | Mutants | Index Medicus
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6. Full Text Semisynthesis and initial characterization of sortase A mutants containing selenocysteine and homocysteine
by Schmohl, Lena and Wagner, Felix Roman and Schümann, Michael and Krause, Eberhard and Schwarzer, Dirk
Bioorganic & medicinal chemistry, ISSN 0968-0896, 06/2015, Volume 23, Issue 12, pp. 2883 - 2889
Protein semisynthesis | Selenocysteine | Homocysteine | Sortase-mediated ligation | Sortase | Expressed protein ligation | Biochemistry & Molecular Biology | Physical Sciences | Chemistry | Chemistry, Organic | Life Sciences & Biomedicine | Pharmacology & Pharmacy | Chemistry, Medicinal | Science & Technology | Cysteine Endopeptidases - chemistry | Selenocysteine - metabolism | Aminoacyltransferases - genetics | Bacterial Proteins - chemistry | Homocysteine - metabolism | Alkylation | Homocysteine - genetics | Solid-Phase Synthesis Techniques | Cysteine Endopeptidases - metabolism | Protein Engineering | Catalysis | Transformation, Genetic | Amino Acid Sequence | Aminoacyltransferases - chemistry | Catalytic Domain | Selenocysteine - chemistry | Bacterial Proteins - genetics | Protein Folding | Selenocysteine - genetics | Escherichia coli - genetics | Cysteine Endopeptidases - genetics | Aminoacyltransferases - metabolism | Bacterial Proteins - metabolism | Homocysteine - chemistry | Mutation | Index Medicus
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7. Full Text Chemo‐enzymatic three‐fragment assembly of semisynthetic proteins
by Schmohl, Lena and Schwarzer, Dirk
Journal of peptide science, ISSN 1075-2617, 02/2014, Volume 20, Issue 2, pp. 145 - 151
sortase‐mediated ligation | phosphopantetheine transferase | protein semisynthesis | FRET reporter | sortase-mediated ligation | Biochemistry & Molecular Biology | Physical Sciences | Chemistry | Chemistry, Analytical | Life Sciences & Biomedicine | Science & Technology | Peptide Fragments - chemistry | Peptide Fragments - metabolism | Chemistry Techniques, Synthetic | Cysteine Endopeptidases - metabolism | Aminoacyltransferases - metabolism | Bacterial Proteins - metabolism | Molecular Structure | Proteins - chemistry | Proteins - chemical synthesis | Fluorescence | Enzymes | Chemotherapy | Peptides | Analysis | Cancer | Index Medicus
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8. Full Text Identification of sortase substrates by specificity profiling
by Schmohl, Lena and Bierlmeier, Jan and von Kügelgen, Nicolai and Kurz, Leonie and Reis, Pascal and Barthels, Fabian and Mach, Pia and Schutkowski, Mike and Freund, Christian and Schwarzer, Dirk
Bioorganic & medicinal chemistry, ISSN 0968-0896, 09/2017, Volume 25, Issue 18, pp. 5002 - 5007
Streptococcus pyogenes | Sortase-mediated ligation | Staphylococcus aureus | Sortase | Protein labeling | Biochemistry & Molecular Biology | Physical Sciences | Chemistry | Chemistry, Organic | Life Sciences & Biomedicine | Pharmacology & Pharmacy | Chemistry, Medicinal | Science & Technology | Recombinant Proteins - metabolism | Amino Acid Sequence | Aminoacyltransferases - genetics | Bacterial Proteins - genetics | Spectrometry, Fluorescence | Substrate Specificity | Chromatography, High Pressure Liquid | Recombinant Proteins - isolation & purification | Cysteine Endopeptidases - metabolism | Peptides - metabolism | Staphylococcus - enzymology | Cysteine Endopeptidases - genetics | Mass Spectrometry | Aminoacyltransferases - metabolism | Bacterial Proteins - metabolism | Peptides - analysis | Enzymes | Bacteria | Catalysis | Glycine | Genomics | Staphylococcus aureus infections | Index Medicus
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9. Full Text Chemical Synthesis and Biological Function of Lipidated Proteins
by Yang, Aimin and Zhao, Lei and Wu, Yao-Wen
12/2014, Topics in Current Chemistry, ISBN 3319191853, Volume 362
Rab | GPI anchor | Diels–Alder ligation | Protein lipidation | Ras | Rheb | Peptide synthesis | Click ligation | MIC ligation | Sortase-mediated protein ligation | Expressed protein ligation | Cell Membrane - chemistry | Proteins - physiology | Lipids - chemistry | Animals | Membrane Proteins - chemistry | Membrane Proteins - physiology | Protein Conformation | Proteins - chemistry | Proteins - chemical synthesis
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10. Full Text Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody–Drug Conjugates
by Gébleux, Rémy and Briendl, Manfred and Grawunder, Ulf and Beerli, Roger R
06/2019, Methods in Molecular Biology, ISBN 9781493995455, Volume 2012
ADC | Anthracycline | Antibody–drug conjugate | Maytansine | SMAC | Auristatin | Site-specific conjugation | Sortase A-mediated ligation | Transpeptidation | Sortase-mediated antibody conjugation | Cysteine Endopeptidases - chemistry | Aminoacyltransferases - chemistry | Antibodies, Monoclonal - pharmacology | Humans | Bacterial Proteins - chemistry | Antibodies, Monoclonal - isolation & purification | Chromatography, High Pressure Liquid | Immunoconjugates - chemistry | Immunoconjugates - immunology | Chromatography, Reverse-Phase | Immunoconjugates - pharmacology | Animals | Drug Compounding | Molecular Structure | Antibodies, Monoclonal - chemistry | Antibodies, Monoclonal - immunology | Immunoconjugates - isolation & purification | Index Medicus
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