Articles

Search Articles

Advanced search
Help

Catalogue

Articles

Databases

X
Search Filters
Format Format
Subjects Subjects
Subjects Subjects
X
Sort by Item Count (A-Z)
Filter by Count
thermococcus - enzymology (420) 420
index medicus (311) 311
biochemistry & molecular biology (289) 289
molecular sequence data (219) 219
amino acid sequence (218) 218
microbiology (210) 210
enzymes (199) 199
thermococcus - genetics (193) 193
escherichia-coli (184) 184
archaeal proteins - metabolism (158) 158
archaeal proteins - genetics (141) 141
enzyme stability (141) 141
temperature (133) 133
archaeal proteins - chemistry (129) 129
kinetics (125) 125
pyrococcus-furiosus (121) 121
recombinant proteins - metabolism (121) 121
substrate specificity (118) 118
biotechnology & applied microbiology (115) 115
cloning, molecular (115) 115
crystal-structure (114) 114
purification (114) 114
archaea (112) 112
hot temperature (108) 108
proteins (103) 103
models, molecular (101) 101
hyperthermophilic archaeon (100) 100
sequence homology, amino acid (97) 97
recombinant proteins - chemistry (96) 96
crystallography, x-ray (94) 94
analysis (88) 88
biophysics (88) 88
recombinant proteins - genetics (85) 85
base sequence (81) 81
biotechnology (80) 80
escherichia coli - genetics (80) 80
research (80) 80
sequence alignment (80) 80
hydrogen-ion concentration (76) 76
gene expression (75) 75
bacteria (74) 74
protein conformation (74) 74
cloning (72) 72
physiological aspects (71) 71
dna (63) 63
expression (63) 63
thermococcus (63) 63
recombinant proteins - isolation & purification (60) 60
life sciences (59) 59
dna-directed dna polymerase - metabolism (55) 55
molecular weight (55) 55
binding sites (54) 54
protein structure, tertiary (54) 54
thermococcus-litoralis (54) 54
enzyme (53) 53
gene (53) 53
escherichia coli (50) 50
phylogeny (50) 50
thermococcus - metabolism (49) 49
genetic aspects (48) 48
mutation (48) 48
crystals (47) 47
polymerase chain reaction (47) 47
hyperthermophile (46) 46
amino acids (45) 45
biochemical research methods (44) 44
chemical properties (44) 44
crystallography (44) 44
biochemistry (43) 43
catalysis (43) 43
crystal structure (42) 42
crystallization (41) 41
dna-directed dna polymerase - genetics (41) 41
genes (40) 40
biochemical-characterization (39) 39
protein (39) 39
archaea - enzymology (38) 38
catalytic domain (38) 38
structure (38) 38
protein folding (37) 37
sequence (37) 37
dna-directed dna polymerase - chemistry (36) 36
hydrolysis (35) 35
metabolism (34) 34
protein binding (34) 34
pyrococcus furiosus - enzymology (34) 34
thermostability (34) 34
bacterial proteins - chemistry (33) 33
biochemistry, general (33) 33
e coli (33) 33
sulfolobus-solfataricus (33) 33
bacteriology (32) 32
dna polymerase (32) 32
dna, archaeal - genetics (32) 32
thermodynamics (32) 32
complex (31) 31
glycoside hydrolases - metabolism (31) 31
identification (31) 31
active-site (30) 30
bacterial proteins - metabolism (30) 30
more...
Library Location Library Location
Language Language
Publication Date Publication Date
Click on a bar to filter by decade
Slide to change publication date range


1. Full Text TK1656, a thermostable l-asparaginase from Thermococcus kodakaraensis, exhibiting highest ever reported enzyme activity
by Chohan, Shahid Mahmood and Rashid, Naeem
Journal of Bioscience and Bioengineering, ISSN 1389-1723, 10/2013, Volume 116, Issue 4, pp. 438 - 443
Two -asparaginase homologs, TK1656 and TK2246, have been found in the genome of The gene encoding TK1656 consists of 984 nucleotides corresponding to a... 
Unfolding | Thermococcus kodakaraensis | l-Asparaginase | Structural stability | Thermal stability | L-Asparaginase | FOOD SCIENCE & TECHNOLOGY | ESCHERICHIA-COLI | CANCER | THERAPY | AFFINITY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PURIFICATION | ACRYLAMIDE | LEUKEMIA | ERWINIA-CAROTOVORA | Asparaginase - genetics | Amino Acid Sequence | Protein Denaturation - drug effects | Temperature | Asparaginase - metabolism | Biocatalysis | Ammonia - metabolism | Protein Unfolding - drug effects | Thermococcus - genetics | Guanidine - pharmacology | Asparaginase - isolation & purification | Enzyme Stability | Molecular Sequence Data | Asparaginase - chemistry | Thermococcus - enzymology | Escherichia coli - genetics | Asparaginase - drug effects | Asparagine - metabolism | Cloning, Molecular | Aspartic Acid - metabolism | Kinetics | Urea - pharmacology | Hydrogen-Ion Concentration | Enzymes | Exhibitions | Genomics
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
2. Full Text Directed evolution of thermotolerant malic enzyme for improved malate production
by Morimoto, Yumi and Honda, Kohsuke and Ye, Xiaoting and Okano, Kenji and Ohtake, Hisao
Journal of Bioscience and Bioengineering, ISSN 1389-1723, 02/2014, Volume 117, Issue 2, pp. 147 - 152
The directed evolution of the thermotolerant NADP(H)-dependent malic enzyme from was conducted to alter the cofactor preference of the enzyme from NADP(H) to... 
Directed evolution | Reaction specificity | Cofactor preference | [formula omitted] fixation | Malic enzyme | fixation | HCO | COENZYME | ACID | MECHANISM | CONVERSION | REGENERATION | FOOD SCIENCE & TECHNOLOGY | HCO3- fixation | PATHWAY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | CARBON-DIOXIDE | FIXATION | PIGEON | Malate Dehydrogenase - metabolism | Biocatalysis | Gene Library | Oxidation-Reduction | Substrate Specificity | Directed Molecular Evolution | Enzyme Assays | Malate Dehydrogenase - genetics | Point Mutation - genetics | Thermococcus - enzymology | Malates - metabolism | Protein Engineering | Pyruvic Acid - metabolism | Kinetics | Malate Dehydrogenase - chemistry | NADP - metabolism | NAD - metabolism | Lactates | Enzymes | Gene mutations | Analysis
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
3. Full Text Hyperthermophilic carbamate kinase stability and anabolic in vitro activity at alkaline pH
by Hennessy, James E and Latter, Melissa J and Fazelinejad, Somayeh and Philbrook, Amy and Bartkus, Daniel M and Kim, Hye-Kyung and Onagi, Hideki and Oakeshott, John G and Scott, Colin and Alissandratos, Apostolos and Easton, Christopher J
Applied and Environmental Microbiology, ISSN 0099-2240, 02/2018, Volume 84, Issue 3
Carbamate kinases catalyze the conversion of carbamate to carbamoyl phosphate, which is readily transformed into other compounds. Carbamate forms spontaneously... 
Hyperthermophiles | Ammonia | Carbamate kinase | Bioremediation | Carbamoyl phosphate | Carbon dioxide | carbon dioxide | bioremediation | carbamate kinase | CO2 | ammonia | DECOMPOSITION | MICROBIOLOGY | MECHANISMS | ORGANIZATION | PHOSPHATE SYNTHETASE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ENZYMES | BIOSYNTHESIS | ARCHAEON PYROCOCCUS-FURIOSUS | carbamoyl phosphate | ARGININE | hyperthermophiles | CARBAMOYLTRANSFERASE | Carbamyl Phosphate - metabolism | Temperature | Pyrococcus furiosus - metabolism | Clostridium tetani - enzymology | Thermococcus - genetics | Phosphotransferases (Carboxyl Group Acceptor) - metabolism | Carbamates - metabolism | Clostridium tetani - genetics | Thermococcus - enzymology | Catalysis | Alkalies - metabolism | Enterococcus faecalis - enzymology | Pyrococcus furiosus - enzymology | Ammonia - metabolism | Enterococcus faecalis - genetics | Enzyme Stability | Models, Molecular | Clostridium tetani - metabolism | Pyrococcus furiosus - genetics | Enterococcus faecalis - metabolism | Protein Conformation | Kinetics | Thermococcus - metabolism | Anabolic Agents - metabolism | Hydrogen-Ion Concentration | Analysis | Physiological aspects | Carbamates | Thermococcus | Hydrogen-ion concentration | Adenosine triphosphate | Phosphates | Enzymes | Stability | High temperature | Kinases | pH effects | Substrates | Thermophiles | Hydrogen ions | Acetyl phosphate | Alkalinity | Carbon capture and storage
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
4. Full Text Conformational Analysis of Processivity Clamps in Solution Demonstrates that Tertiary Structure Does Not Correlate with Protein Dynamics
by Fang, Jing and Nevin, Philip and Kairys, Visvaldas and Venclovas, Česlovas and Engen, John R and Beuning, Penny J
Structure, ISSN 0969-2126, 04/2014, Volume 22, Issue 4, pp. 572 - 581
The relationship between protein sequence, structure, and dynamics has been elusive. Here, we report a comprehensive analysis using an in-solution experimental... 
MOLECULAR-DYNAMICS | PCNA | CELL NUCLEAR ANTIGEN | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | EXCHANGE-MASS-SPECTROMETRY | DNA-POLYMERASE-III | CELL BIOLOGY | BIOPHYSICS | SLIDING-CLAMP | TRANSITIONS | HYDROGEN-EXCHANGE | Arabidopsis - enzymology | DNA Polymerase III - genetics | Species Specificity | Humans | Protein Multimerization | Bacteriophage T4 - chemistry | Half-Life | Thermococcus - enzymology | Bacteriophage T4 - enzymology | Protein Structure, Tertiary | Gene Expression | Arabidopsis - chemistry | Escherichia coli - enzymology | Protein Structure, Secondary | Thermococcus - chemistry | Recombinant Proteins - chemistry | Recombinant Proteins - genetics | Escherichia coli - chemistry | Molecular Dynamics Simulation | Protein Folding | Saccharomyces cerevisiae - chemistry | DNA Polymerase III - chemistry | Protein Binding | Saccharomyces cerevisiae - enzymology | Structural Homology, Protein | Proteins | Hydrogen | Dynamic structural analysis | Correlation | Correlation analysis | Dynamics | Helices | Clamps | Three dimensional | hydrogen exchange mass spectrometry | DNA replication | alpha-helix
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
5. Hyperthermophilic enzymes
by Kelly, Robert M., 1953 and Adams, Michael W. W., 1954
2001, Methods in enzymology, ISBN 0121822311, Volume 330, 331, < 1-2 > v.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymologyis one of the most highly respected publications in the field of... 
Enzymes | Effect of heat on | Microorganisms | Effect of temperature on | Proteolytic enzymes | Thermophilic microorganisms
Book
Details down arrow
6. Full Text TK1299, a highly thermostable NAD(P)H oxidase from Thermococcus kodakaraensis exhibiting higher enzymatic activity with NADPH
by Nisar, Muhammad Atif and Rashid, Naeem and Bashir, Qamar and Gardner, Qurra-tul-Ann Afza and Shafiq, Muhammad Hassan and Akhtar, Muhammad
Journal of Bioscience and Bioengineering, ISSN 1389-1723, 07/2013, Volume 116, Issue 1, pp. 39 - 44
Seven nicotinamide adenine dinucleotide oxidase homologs have been found in the genome of The gene encoding one of them, TK1299, consisted of 1326 nucleotides,... 
Hyperthermophile | Thermococcus kodakaraensis | Thermostable | Cloning | NAD(P)H oxidase | Circular dichroism | OXIDATIVE STRESS | FOOD SCIENCE & TECHNOLOGY | OXIDOREDUCTASE | DISULFIDE REDUCTASE | ARCHAEON | PYROCOCCUS-FURIOSUS | METABOLISM | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ANAEROBIC HYPERTHERMOPHILES | FLAVOPROTEINS | GENE CLONING | INSIGHTS | NADPH Oxidases - chemistry | Amino Acid Sequence | Temperature | Oxidation-Reduction | Thermococcus - genetics | Enzyme Stability | NADPH Oxidases - metabolism | Molecular Sequence Data | Dinitrocresols - analysis | Sequence Homology, Amino Acid | Thermococcus - enzymology | Escherichia coli - genetics | NADPH Oxidases - genetics | NADP - metabolism | Hydrogen-Ion Concentration
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
7. Full Text Next-generation sequencing-based analysis of reverse transcriptase fidelity
by Yasukawa, Kiyoshi and Iida, Kei and Okano, Hiroyuki and Hidese, Ryota and Baba, Misato and Yanagihara, Itaru and Kojima, Kenji and Takita, Teisuke and Fujiwara, Shinsuke
Biochemical and Biophysical Research Communications, ISSN 0006-291X, 10/2017, Volume 492, Issue 2, pp. 147 - 153
In this study, we devised a simple and rapid method to analyze fidelity of reverse transcriptase (RT) using next-generation sequencing (NGS). The method... 
Fidelity | cDNA | Reverse transcriptase | NGS | Error rate | HIV-1 | RNA | BIOCHEMISTRY & MOLECULAR BIOLOGY | DNA-POLYMERASE | DNA/RNA HELICASE | SITE-DIRECTED MUTAGENESIS | ACCURACY | BIOPHYSICS | MUTATIONS | PCR | Moloney murine leukemia virus - genetics | Thermococcus - genetics | DNA, Complementary - genetics | HIV Reverse Transcriptase - genetics | HIV-1 - genetics | DNA-Directed DNA Polymerase - genetics | Thermococcus - enzymology | Base Sequence | Reverse Transcriptase Polymerase Chain Reaction - methods | RNA-Directed DNA Polymerase - chemistry | RNA-Directed DNA Polymerase - genetics | HIV-1 - enzymology | High-Throughput Nucleotide Sequencing - methods | Moloney murine leukemia virus - enzymology | DNA polymerases | Analysis | Leukemia
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
8. Full Text One-Pot Biosynthesis of High-Concentration α-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes
by Zhou, Wei and You, Chun and Ma, Hongwu and Ma, Yanhe and Zhang, Y.-H. Percival
Journal of Agricultural and Food Chemistry, ISSN 0021-8561, 03/2016, Volume 64, Issue 8, pp. 1777 - 1783
Hydroperoxide lyase (HPL, E.C. 4.1.2.) is the major enzyme in the biosynthesis of natural volatile aldehydes and alcohols in plants, however, little was known... 
4-glucanotransferase | cascade enzyme biocatalysis | enzyme cocktail | α-glucose 1-phosphate | isoamylase | α-glucan phosphorylase | SUCROSE PHOSPHORYLASE | FOOD SCIENCE & TECHNOLOGY | ESCHERICHIA-COLI | suppressive subtractive hybridization (SSH) | PYROCOCCUS-FURIOSUS | SULFOLOBUS-TOKODAII | ARCHAEON THERMOCOCCUS-LITORALIS | Camellia sinensis | MALTODEXTRIN-PHOSPHORYLASE | hydroperoxide lyase | GLUCAN PHOSPHORYLASE | AGRICULTURE, MULTIDISCIPLINARY | Ectropis obliqua | LEUCONOSTOC-MESENTEROIDES | CHEMISTRY, APPLIED | THERMOSTABLE 4-ALPHA-GLUCANOTRANSFERASE | THERMUS-THERMOPHILUS | induced defense response | Phosphorylases - chemistry | Biocatalysis | Sulfolobus - enzymology | Thermococcus - chemistry | Archaeal Proteins - chemistry | Bacterial Proteins - chemistry | Glucosephosphates - chemistry | Hot Temperature | Thermotoga maritima - enzymology | Starch - chemistry | Sulfolobus - chemistry | Thermococcus - enzymology | Isoamylase - chemistry | Glucosyltransferases - chemistry | Thermotoga maritima - chemistry | Hydrogen-Ion Concentration
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
9. Full Text Novel Families of Archaeo-Eukaryotic Primases Associated with Mobile Genetic Elements of Bacteria and Archaea
by Kazlauskas, Darius and Sezonov, Guennadi and Charpin, Nicole and Venclovas, ÄŒeslovas and Forterre, Patrick and Krupovic, Mart
Journal of Molecular Biology, ISSN 0022-2836, 03/2018, Volume 430, Issue 5, pp. 737 - 750
Cellular organisms in different domains of life employ structurally unrelated, non-homologous DNA primases for synthesis of a primer for DNA replication.... 
DNA replication | structural modeling | evolution | helicases | Thermococcus plasmids | STRANDED-DNA | MCM-LIKE HELICASE | ORIGIN | REPLICATION PROTEINS | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | PROTEIN FAMILIES | POLYMERASE | PLASMID RSF1010 | WEB SERVER | LARGE DNA VIRUSES | Amino Acid Sequence | Catalytic Domain | Interspersed Repetitive Sequences | Thermococcus - genetics | DNA Primase - metabolism | Models, Molecular | DNA Replication | DNA Primase - classification | Archaea - enzymology | Bacteria - genetics | DNA Helicases - metabolism | Sequence Alignment | Eukaryota - genetics | Protein Domains | Bacteria - enzymology | Protein Conformation | Archaea - genetics | Eukaryota - enzymology | Evolution, Molecular | Life Sciences | NHEJ, non-homologous end joining | LigD, ligase D | HTH, helix-turn-helix | MGEs, mobile genetic elements | pVOG, prokaryotic virus orthologous groups | ZnBD, Zn-binding domain | WH, winged-helix | AEPs, archaeo-eukaryotic primases | PriCT-2, Primase-C Terminal-1 | PriCT-1 | DUF, domain of unknown function
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
10. Full Text Loading and activation of DNA replicative helicases: the key step of initiation of DNA replication
by Li, Yan and Araki, Hiroyuki
Genes to Cells, ISSN 1356-9597, 04/2013, Volume 18, Issue 4, pp. 266 - 277
Evolution has led to diversification of all living organisms from a common ancestor. Consequently, all living organisms use a common method to duplicate their... 
MCM2-7 HELICASE | THERMOCOCCUS KODAKARENSIS | BACILLUS-SUBTILIS | BUDDING YEAST | ORIGIN RECOGNITION COMPLEX | ESCHERICHIA-COLI | IN-VIVO | GENETICS & HEREDITY | STAPHYLOCOCCUS-AUREUS | SINGLE-STRANDED-DNA | SACCHAROMYCES-CEREVISIAE | CELL BIOLOGY | DNA, Bacterial - metabolism | DNA, Bacterial - biosynthesis | Humans | DNA Replication | Archaea - enzymology | Bacteria - genetics | DNA Helicases - metabolism | Animals | Eukaryota - genetics | Replication Origin | Bacteria - enzymology | Bacterial Proteins - metabolism | Archaea - genetics | DNA-Directed DNA Polymerase - metabolism | Eukaryota - enzymology | DNA Helicases - genetics | Evolution, Molecular | Genetic research | DNA replication | DNA | Proteins | Deoxyribonucleic acid--DNA | Reviews
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
11. Full Text Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates
by Güixens-Gallardo, Pedro and Hocek, Michal and Perlíková, Pavla
Bioorganic & Medicinal Chemistry Letters, ISSN 0960-894X, 01/2016, Volume 26, Issue 2, pp. 288 - 291
A simple and elegant method for inhibition of non-templated nucleotide addition by DNA polymerases and for following DNA 3′-heterogeneity in enzymatic DNA... 
DNA polymerases | Nucleotide addition | Primer extension | Oligonucleotides | Twisted intercalating nucleic acid | SUGAR RECOGNITION | CHEMISTRY, MEDICINAL | CHEMISTRY, ORGANIC | TRIPHOSPHATES | ARCHAEON | APTAZYMES | ELECTROCHEMICAL DETECTION | AMPLIFICATION | BEARING | END | APTAMERS | PCR | Biotin - chemistry | DNA - genetics | DNA - chemistry | Geobacillus stearothermophilus - enzymology | Pyrenes - chemistry | Thermococcus - enzymology | Thermus - enzymology | DNA-Directed DNA Polymerase - chemistry | DNA-Directed DNA Polymerase - genetics | Intercalating Agents - chemistry | Trityl Compounds - chemistry | Pyrococcus | DNA
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
12. Full Text Thermophilic archaeal enzymes and applications in biocatalysis
by Littlechild, Jennifer A
Biochemical Society Transactions, ISSN 0300-5127, 02/2011, Volume 39, Issue 1, pp. 155 - 158
Thermophilic enzymes have advantages for their use in commercial applications and particularly for the production of chiral compounds to produce optically pure... 
Immobilization | Biocatalysis | Thermophile | Archaea | PYRROLIDONE CARBOXYL PEPTIDASE | immobilization | THERMOCOCCUS-LITORALIS | X-RAY DIFFRACTION | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | SULFOLOBUS-SOLFATARICUS | THERMOSTABLE L-AMINOACYLASE | biocatalysis | thermophile | ALCOHOL-DEHYDROGENASE | AEROPYRUM-PERNIX | PYROCOCCUS-HORIKOSHII | HALOACID DEHALOGENASE | Alcohol Dehydrogenase - metabolism | Amidohydrolases - metabolism | Archaeal Proteins - metabolism | Hydrolases - metabolism | Green Chemistry Technology | Aminohydrolases - chemistry | Archaeal Proteins - chemistry | Models, Molecular | Transaminases - chemistry | Sulfolobus solfataricus - enzymology | Amidohydrolases - chemistry | Archaea - enzymology | Thermococcus - enzymology | Transaminases - metabolism | Aeropyrum - enzymology | Aminohydrolases - metabolism | Protein Conformation | Hydrolases - chemistry | Alcohol Dehydrogenase - chemistry
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
13. Full Text Exploration of extremophiles for high temperature biotechnological processes
by Elleuche, Skander and Schäfers, Christian and Blank, Saskia and Schröder, Carola and Antranikian, Garabed
Current Opinion in Microbiology, ISSN 1369-5274, 2015, Volume 25, pp. 113 - 119
Graphical abstract 
Pathology | CLONING | RECOMBINANT | ENZYMES | SULFOLOBUS-SOLFATARICUS | BIOCHEMICAL-CHARACTERIZATION | MICROBIOLOGY | EXPRESSION | PROTEASE | THERMOCOCCUS-KODAKARENSIS KOD1 | THERMUS-THERMOPHILUS HB27 | ALPHA-AMYLASE | Enzymes - chemistry | Biotechnology | Metagenomics | Enzymes - economics | Cellulases - metabolism | Enzymes - isolation & purification | Bacteria - enzymology | Industrial Microbiology - methods | Hot Temperature | Archaea - enzymology | Enzymes - metabolism | Peptide Hydrolases - metabolism
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
14. Full Text Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase
by Okano, Hiroyuki and Katano, Yuta and Baba, Misato and Fujiwara, Ayako and Fujiwara, Shinsuke and Hidese, Ryota and Yanagihara, Itaru and Hayashi, Tsukasa and Kojima, Kenji and Takita, Teisuke and Yasukawa, Kiyoshi
Enzyme and Microbial Technology, ISSN 0141-0229, 01/2017, Volume 96, pp. 111 - 120
Detection of mRNA is a valuable method for monitoring the specific gene expression. In this study, we devised a novel cDNA synthesis method using three... 
Helicase | Taguchi’s method | Reverse transcriptase | DNA polymerase | cDNA synthesis | Taguchi's method | MURINE LEUKEMIA-VIRUS | AMPLIFICATION | REDUCTION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | BACTERIAL FAMILY | INCREASE | OPTIMIZATION | PRIMER | MUTATIONS | PCR | Recombinant Proteins - metabolism | Moloney murine leukemia virus - genetics | Gene Expression | Temperature | Oligonucleotide Array Sequence Analysis | RNA - analysis | RNA-Directed DNA Polymerase - metabolism | Thermococcus - genetics | DNA, Complementary - genetics | Enzyme Stability | Recombinant Proteins - genetics | DNA-Directed DNA Polymerase - genetics | RNA - genetics | Thermococcus - enzymology | Base Sequence | Protein Engineering | RNA-Directed DNA Polymerase - genetics | DNA, Complementary - biosynthesis | RNA Helicases - genetics | DNA-Directed DNA Polymerase - metabolism | Gram-Negative Anaerobic Straight, Curved, and Helical Rods - enzymology | Gram-Negative Anaerobic Straight, Curved, and Helical Rods - genetics | DNA Helicases - genetics | Moloney murine leukemia virus - enzymology | DNA polymerases | Enzymes | RNA | DNA | Leukemia | Genetically modified organisms | Genetic research | Genetic engineering | Gene expression
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
15. Full Text Family B DNA polymerase from a hyperthermophilic archaeon Pyrobaculum calidifontis: Cloning, characterization and PCR application
by Ali, Syed Farhat and Rashid, Naeem and Imanaka, Tadayuki and Akhtar, Muhammad
Journal of Bioscience and Bioengineering, ISSN 1389-1723, 2011, Volume 112, Issue 2, pp. 118 - 123
The 2352 bp gene coding for 783 amino acid family B DNA polymerase from was cloned and expressed in . Expression of the gene resulted in the production of -Pol... 
Polymerase chain reaction | Hyperthermophile | DNA polymerase | Pyrobaculum calidifontis | Exonuclease activity | Archaea | THERMOCOCCUS-LITORALIS | FOOD SCIENCE & TECHNOLOGY | THERMUS-AQUATICUS | REPLICATION | ENZYME | ALIGNMENT | FIDELITY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | EXPRESSION | Recombinant Proteins - metabolism | Amino Acid Sequence | Pyrobaculum - enzymology | Half-Life | Recombinant Proteins - genetics | DNA-Directed DNA Polymerase - chemistry | DNA-Directed DNA Polymerase - genetics | Recombinant Proteins - isolation & purification | Escherichia coli - genetics | Cloning, Molecular | Escherichia coli - metabolism | Polymerase Chain Reaction | Pyrobaculum - genetics | DNA-Directed DNA Polymerase - metabolism | Proteins | DNA polymerases | Enzymes | Denaturation | Cloning | DNA
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights
16. Full Text An in vitro enzyme system for the production of myo-inositol from starch
by Fujisawa, Tomoko and Fujinaga, Shohei and Atomi, Haruyuki
Applied and Environmental Microbiology, ISSN 0099-2240, 08/2017, Volume 83, Issue 16
We developed an in vitro enzyme system to produce myo-inositol from starch. Four enzymes were used, maltodextrin phosphorylase (MalP), phosphoglucomutase... 
Hyperthermophiles | Thermococcus | Maltodextrin phosphorylase | Archaea | myo-inositol | PHOSPHOGLUCOMUTASE | TRANSFORMATION | THERMOCOCCUS-KODAKARAENSIS | ARCHAEOGLOBUS-FULGIDUS | ESCHERICHIA-COLI | MICROBIOLOGY | archaea | COMPLETE GENOME SEQUENCE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | maltodextrin phosphorylase | BIOSYNTHESIS | PURIFICATION | SP-NOV | hyperthermophiles | THERMOTOGA-MARITIMA | Archaeal Proteins - metabolism | Phosphoric Monoester Hydrolases - genetics | Inositol - metabolism | Intramolecular Lyases - metabolism | Archaeal Proteins - chemistry | Enzyme Stability | Starch - metabolism | Archaeoglobus fulgidus - enzymology | Starch - chemistry | Intramolecular Lyases - genetics | Thermococcus - enzymology | Inositol - chemistry | Archaeal Proteins - genetics | Phosphoric Monoester Hydrolases - metabolism | Intramolecular Lyases - chemistry | NAD - metabolism | Phosphoric Monoester Hydrolases - chemistry | Usage | Toxicity testing | Genetic aspects | Research | In vitro | Phosphates | Enzymes | Heat treatment | Potatoes | Starch | Inositol-3-phosphate synthase | Effects | Myo-Inositol-1 (or 4)-monophosphatase | Cells | Phosphorylase | Phosphoglucomutase | Proteins | NAD | Amylose | E coli | Maltodextrin | Pullulanase
Journal Article
Details down arrow
Digital rights down arrow
loading Loading Rights