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thermococcus - enzymology (426) 426
science & technology (411) 411
life sciences & biomedicine (383) 383
biochemistry & molecular biology (241) 241
thermococcus - genetics (201) 201
molecular sequence data (176) 176
amino acid sequence (175) 175
microbiology (165) 165
enzymes (141) 141
archaeal proteins - metabolism (137) 137
archaeal proteins - genetics (128) 128
archaeal proteins - chemistry (112) 112
enzyme stability (112) 112
kinetics (101) 101
temperature (99) 99
recombinant proteins - metabolism (97) 97
cloning, molecular (92) 92
substrate specificity (91) 91
models, molecular (90) 90
archaea (88) 88
biological and medical sciences (87) 87
fundamental and applied biological sciences. psychology (87) 87
biotechnology & applied microbiology (85) 85
crystallography, x-ray (85) 85
hot temperature (83) 83
biotechnology (82) 82
biophysics (80) 80
sequence homology, amino acid (76) 76
recombinant proteins - chemistry (74) 74
analysis (70) 70
base sequence (70) 70
proteins (67) 67
recombinant proteins - genetics (67) 67
protein conformation (65) 65
research (64) 64
sequence alignment (64) 64
gene expression (63) 63
escherichia coli - genetics (62) 62
thermococcus (62) 62
hydrogen-ion concentration (61) 61
bacteriology (60) 60
physical sciences (58) 58
thermococcus - metabolism (53) 53
bacteria (51) 51
binding sites (51) 51
dna-directed dna polymerase - metabolism (51) 51
physiological aspects (51) 51
life sciences (50) 50
dna (48) 48
recombinant proteins - isolation & purification (48) 48
space life sciences (46) 46
mutation (44) 44
escherichia coli (43) 43
polymerase chain reaction (42) 42
biochemical research methods (41) 41
protein structure, tertiary (41) 41
dna-directed dna polymerase - genetics (39) 39
phylogeny (39) 39
crystal structure (38) 38
dna-directed dna polymerase - chemistry (38) 38
crystals (37) 37
molecular weight (37) 37
genetic aspects (36) 36
amino acids (35) 35
biochemistry (35) 35
chemical properties (35) 35
crystallography (35) 35
dna polymerase (34) 34
thermococcus - chemistry (34) 34
catalysis (33) 33
chemistry (33) 33
hyperthermophile (33) 33
protein binding (33) 33
crystallization (32) 32
thermococcus - growth & development (32) 32
dna polymerases (31) 31
dna, archaeal - genetics (31) 31
catalytic domain (30) 30
genes (30) 30
protein folding (30) 30
structure (30) 30
research article (28) 28
miscellaneous (26) 26
protein structure, secondary (26) 26
sequence analysis, dna (26) 26
thermococcus kodakaraensis (26) 26
genetics (25) 25
mutagenesis, site-directed (25) 25
biochemistry, general (24) 24
circular dichroism (24) 24
e coli (24) 24
escherichia coli - metabolism (24) 24
archaeal proteins - isolation & purification (23) 23
cell biology (23) 23
deoxyribonucleic acid--dna (23) 23
dna replication (23) 23
genes, archaeal (23) 23
hydrolysis (23) 23
phosphates (23) 23
thermodynamics (23) 23
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1. Full Text TK1656, a thermostable l-asparaginase from Thermococcus kodakaraensis, exhibiting highest ever reported enzyme activity
by Chohan, Shahid Mahmood and Rashid, Naeem
Journal of bioscience and bioengineering, ISSN 1389-1723, 10/2013, Volume 116, Issue 4, pp. 438 - 443
Unfolding | Thermococcus kodakaraensis | l-Asparaginase | Structural stability | Thermal stability | L-Asparaginase | Food Science & Technology | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biotechnology | Biological and medical sciences | Asparaginase - genetics | Amino Acid Sequence | Protein Denaturation - drug effects | Temperature | Asparaginase - metabolism | Biocatalysis | Ammonia - metabolism | Protein Unfolding - drug effects | Thermococcus - genetics | Guanidine - pharmacology | Asparaginase - isolation & purification | Enzyme Stability | Molecular Sequence Data | Asparaginase - chemistry | Thermococcus - enzymology | Escherichia coli - genetics | Asparaginase - drug effects | Asparagine - metabolism | Cloning, Molecular | Aspartic Acid - metabolism | Kinetics | Urea - pharmacology | Hydrogen-Ion Concentration | Enzymes | Exhibitions | Genomics | Index Medicus
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2. Full Text Hyperthermophilic carbamate kinase stability and anabolic in vitro activity at alkaline pH
by Hennessy, James E and Latter, Melissa J and Fazelinejad, Somayeh and Philbrook, Amy and Bartkus, Daniel M and Kim, Hye-Kyung and Onagi, Hideki and Oakeshott, John G and Scott, Colin and Alissandratos, Apostolos and Easton, Christopher J
Applied and environmental microbiology, ISSN 0099-2240, 02/2018, Volume 84, Issue 3
Hyperthermophiles | Ammonia | Carbamate kinase | Bioremediation | Carbamoyl phosphate | Carbon dioxide | Life Sciences & Biomedicine | Microbiology | Biotechnology & Applied Microbiology | Science & Technology | Carbamyl Phosphate - metabolism | Temperature | Pyrococcus furiosus - metabolism | Clostridium tetani - enzymology | Thermococcus - genetics | Phosphotransferases (Carboxyl Group Acceptor) - metabolism | Carbamates - metabolism | Clostridium tetani - genetics | Thermococcus - enzymology | Catalysis | Alkalies - metabolism | Enterococcus faecalis - enzymology | Pyrococcus furiosus - enzymology | Ammonia - metabolism | Enterococcus faecalis - genetics | Enzyme Stability | Models, Molecular | Clostridium tetani - metabolism | Pyrococcus furiosus - genetics | Enterococcus faecalis - metabolism | Protein Conformation | Kinetics | Thermococcus - metabolism | Anabolic Agents - metabolism | Hydrogen-Ion Concentration | Analysis | Physiological aspects | Carbamates | Thermococcus | Hydrogen-ion concentration | Adenosine triphosphate | Phosphates | Enzymes | Stability | High temperature | Kinases | pH effects | Substrates | Thermophiles | Hydrogen ions | Acetyl phosphate | Alkalinity | Carbon capture and storage | Index Medicus
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3. Full Text Conformational Analysis of Processivity Clamps in Solution Demonstrates that Tertiary Structure Does Not Correlate with Protein Dynamics
by Fang, Jing and Nevin, Philip and Kairys, Visvaldas and Venclovas, Česlovas and Engen, John R and Beuning, Penny J
Structure (London), ISSN 0969-2126, 04/2014, Volume 22, Issue 4, pp. 572 - 581
Biochemistry & Molecular Biology | Biophysics | Life Sciences & Biomedicine | Science & Technology | Cell Biology | Arabidopsis - enzymology | DNA Polymerase III - genetics | Species Specificity | Humans | Protein Multimerization | Bacteriophage T4 - chemistry | Half-Life | Thermococcus - enzymology | Bacteriophage T4 - enzymology | Protein Structure, Tertiary | Gene Expression | Arabidopsis - chemistry | Escherichia coli - enzymology | Protein Structure, Secondary | Thermococcus - chemistry | Recombinant Proteins - chemistry | Recombinant Proteins - genetics | Escherichia coli - chemistry | Molecular Dynamics Simulation | Protein Folding | Saccharomyces cerevisiae - chemistry | DNA Polymerase III - chemistry | Protein Binding | Saccharomyces cerevisiae - enzymology | Structural Homology, Protein | Proteins | Hydrogen | Index Medicus | Dynamic structural analysis | Correlation | Correlation analysis | Dynamics | Helices | Clamps | Three dimensional | hydrogen exchange mass spectrometry | DNA replication | alpha-helix | PCNA
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4. Full Text Directed evolution of thermotolerant malic enzyme for improved malate production(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
by Morimoto Yumi and Honda Kohsuke and Ye Xiaoting and Okano Kenji and Ohtake Hisao
Journal of bioscience and bioengineering, ISSN 1389-1723, 02/2014, Volume 117, Issue 2, pp. 147 - 152
fixation | Directed evolution | Reaction specificity | Cofactor preference | HCO | Malic enzyme | Food Science & Technology | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biotechnology | Protein engineering | Biological and medical sciences | Methods. Procedures. Technologies | Malate Dehydrogenase - metabolism | Biocatalysis | Gene Library | Oxidation-Reduction | Substrate Specificity | Directed Molecular Evolution | Enzyme Assays | Malate Dehydrogenase - genetics | Point Mutation - genetics | Thermococcus - enzymology | Malates - metabolism | Protein Engineering | Pyruvic Acid - metabolism | Kinetics | Malate Dehydrogenase - chemistry | NADP - metabolism | NAD - metabolism | Lactates | Enzymes | Gene mutations | Analysis | Index Medicus
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5. Full Text One-Pot Biosynthesis of High-Concentration α‑Glucose 1‑Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes
by Zhou, Wei and You, Chun and Ma, Hongwu and Ma, Yanhe and Zhang, Y.-H. Percival
Journal of agricultural and food chemistry, ISSN 0021-8561, 03/2016, Volume 64, Issue 8, pp. 1777 - 1783
4-glucanotransferase | cascade enzyme biocatalysis | enzyme cocktail | α-glucose 1-phosphate | isoamylase | α-glucan phosphorylase | Physical Sciences | Chemistry | Life Sciences & Biomedicine | Chemistry, Applied | Food Science & Technology | Agriculture, Multidisciplinary | Agriculture | Science & Technology | Phosphorylases - chemistry | Biocatalysis | Sulfolobus - enzymology | Thermococcus - chemistry | Archaeal Proteins - chemistry | Bacterial Proteins - chemistry | Glucosephosphates - chemistry | Hot Temperature | Thermotoga maritima - enzymology | Starch - chemistry | Sulfolobus - chemistry | Thermococcus - enzymology | Isoamylase - chemistry | Glucosyltransferases - chemistry | Thermotoga maritima - chemistry | Hydrogen-Ion Concentration | Index Medicus
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6. Full Text Next-generation sequencing-based analysis of reverse transcriptase fidelity
by Yasukawa, Kiyoshi and Iida, Kei and Okano, Hiroyuki and Hidese, Ryota and Baba, Misato and Yanagihara, Itaru and Kojima, Kenji and Takita, Teisuke and Fujiwara, Shinsuke
Biochemical and biophysical research communications, ISSN 0006-291X, 10/2017, Volume 492, Issue 2, pp. 147 - 153
Fidelity | cDNA | Reverse transcriptase | NGS | Error rate | Biochemistry & Molecular Biology | Biophysics | Life Sciences & Biomedicine | Science & Technology | Moloney murine leukemia virus - genetics | Thermococcus - genetics | DNA, Complementary - genetics | HIV Reverse Transcriptase - genetics | HIV-1 - genetics | DNA-Directed DNA Polymerase - genetics | Thermococcus - enzymology | Base Sequence | Reverse Transcriptase Polymerase Chain Reaction - methods | RNA-Directed DNA Polymerase - chemistry | RNA-Directed DNA Polymerase - genetics | HIV-1 - enzymology | High-Throughput Nucleotide Sequencing - methods | Moloney murine leukemia virus - enzymology | DNA polymerases | RNA | Analysis | Leukemia | Index Medicus
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7. Full Text TK1299, a highly thermostable NAD(P)H oxidase from Thermococcus kodakaraensis exhibiting higher enzymatic activity with NADPH
by Nisar, Muhammad Atif and Rashid, Naeem and Bashir, Qamar and Gardner, Qurra-tul-Ann Afza and Shafiq, Muhammad Hassan and Akhtar, Muhammad
Journal of bioscience and bioengineering, ISSN 1389-1723, 07/2013, Volume 116, Issue 1, pp. 39 - 44
Hyperthermophile | Thermococcus kodakaraensis | Thermostable | Cloning | NAD(P)H oxidase | Circular dichroism | Food Science & Technology | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biotechnology | Biological and medical sciences | NADPH Oxidases - chemistry | Amino Acid Sequence | Temperature | Oxidation-Reduction | Thermococcus - genetics | Enzyme Stability | NADPH Oxidases - metabolism | Molecular Sequence Data | Dinitrocresols - analysis | Sequence Homology, Amino Acid | Thermococcus - enzymology | Escherichia coli - genetics | NADPH Oxidases - genetics | NADP - metabolism | Hydrogen-Ion Concentration | Index Medicus
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8. Full Text Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates
by Güixens-Gallardo, Pedro and Hocek, Michal and Perlíková, Pavla
Bioorganic & medicinal chemistry letters, ISSN 0960-894X, 01/2016, Volume 26, Issue 2, pp. 288 - 291
DNA polymerases | Nucleotide addition | Primer extension | Oligonucleotides | Twisted intercalating nucleic acid | Physical Sciences | Chemistry | Chemistry, Organic | Life Sciences & Biomedicine | Pharmacology & Pharmacy | Chemistry, Medicinal | Science & Technology | Biotin - chemistry | DNA - genetics | DNA - chemistry | Geobacillus stearothermophilus - enzymology | Pyrenes - chemistry | Thermococcus - enzymology | Thermus - enzymology | DNA-Directed DNA Polymerase - chemistry | DNA-Directed DNA Polymerase - genetics | Intercalating Agents - chemistry | Trityl Compounds - chemistry | Pyrococcus | DNA | Index Medicus
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9. Full Text Synthetic evolutionary origin of a proofreading reverse transcriptase
by Ellefson, Jared W and Gollihar, Jimmy and Shroff, Raghav and Shivram, Haridha and Iyer, Vishwanath R and Ellington, Andrew D
Science (American Association for the Advancement of Science), ISSN 0036-8075, 06/2016, Volume 352, Issue 6293, pp. 1590 - 1593
Science & Technology - Other Topics | Multidisciplinary Sciences | Science & Technology | Protein Structure, Tertiary | Models, Molecular | Templates, Genetic | Directed Molecular Evolution | Phylogeny | RNA - chemistry | RNA - genetics | Exonucleases - chemistry | Thermococcus - enzymology | DNA Mutational Analysis | DNA Mismatch Repair | RNA-Directed DNA Polymerase - chemistry | RNA-Directed DNA Polymerase - genetics | DNA, Complementary - biosynthesis | RNA-Directed DNA Polymerase - classification | Evolution, Molecular | Pyrococcus furiosus - enzymology | RNA sequencing | Genetic transcription | Nucleotide sequencing | Observations | Methods | DNA sequencing | Proteins | Enzymes | DNA polymerase | Ribonucleic acid--RNA | Index Medicus | Origins | Ribonucleic acids | Biological evolution | Evolutionary | Deoxyribonucleic acid
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10. Full Text ATP-free biosynthesis of a high-energy phosphate metabolite fructose 1,6-diphosphate by in vitro metabolic engineering
by Wang, Wei and Liu, Meixia and You, Chun and Li, Zhimin and Zhang, Yi-Heng Percival
Metabolic engineering, ISSN 1096-7176, 07/2017, Volume 42, pp. 168 - 174
Fructose 1,6-diphosphate | In vitro metabolic engineering | Sugar phosphate | Enzyme cocktail | Hyperthermophilic enzyme | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Metabolic Engineering | Thermococcus - genetics | Bacterial Proteins - genetics | Thermotoga maritima - genetics | Thermotoga maritima - enzymology | Fructosediphosphates - genetics | Thermus thermophilus | Thermococcus - enzymology | Escherichia coli - genetics | Fructosediphosphates - biosynthesis | Escherichia coli - metabolism | Archaeal Proteins - genetics | Bacterial Proteins - biosynthesis | Archaeal Proteins - biosynthesis | Phosphates | Enzymes | Metabolites | Fine chemicals | Manufacturing costs | Precipitation (Meteorology) | Catalysis | Glucose | Fructose | Dextrose | Index Medicus
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11. Full Text Improving Polymerase Activity with Unnatural Substrates by Sampling Mutations in Homologous Protein Architectures
by Dunn, Matthew R and Otto, Carine and Fenton, Kathryn E and Chaput, John C
ACS chemical biology, ISSN 1554-8929, 05/2016, Volume 11, Issue 5, pp. 1210 - 1219
Life Sciences & Biomedicine | Biochemistry & Molecular Biology | Science & Technology | Amino Acid Sequence | Tetroses - metabolism | Bacteria - metabolism | Bacteria - chemistry | Mutagenesis, Site-Directed | Thermococcus - chemistry | Thermococcus - genetics | Models, Molecular | Substrate Specificity | Nucleic Acids - metabolism | DNA-Directed DNA Polymerase - chemistry | Tetroses - chemistry | Bacteria - genetics | DNA-Directed DNA Polymerase - genetics | Nucleic Acids - chemistry | Thermococcus - enzymology | Databases, Protein | Bacteria - enzymology | Protein Conformation | DNA-Directed DNA Polymerase - metabolism | Mutation | Thermococcus - metabolism | Index Medicus
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12. Full Text Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea
by Ishino, Sonoko and Nishi, Yuki and Oda, Soichiro and Uemori, Takashi and Sagara, Takehiro and Takatsu, Nariaki and Yamagami, Takeshi and Shirai, Tsuyoshi and Ishino, Yoshizumi
Nucleic acids research, ISSN 0305-1048, 03/2016, Volume 44, Issue 7, pp. 2977 - 2986
Life Sciences & Biomedicine | Biochemistry & Molecular Biology | Science & Technology | Archaeal Proteins - metabolism | Recombinant Proteins - metabolism | Archaeal Proteins - isolation & purification | Substrate Specificity | Base Pair Mismatch | Hot Temperature | Endodeoxyribonucleases - isolation & purification | Recombinant Proteins - isolation & purification | Endodeoxyribonucleases - genetics | Thermococcus - enzymology | Endodeoxyribonucleases - metabolism | Archaeal Proteins - genetics | Mutation | Proliferating Cell Nuclear Antigen - metabolism | DNA Cleavage | Pyrococcus furiosus - enzymology | Index Medicus | NAR Breakthrough
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