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1. Full Text Plaque assay for murine norovirus
by Gonzalez-Hernandez, Mariam B and Bragazzi Cunha, Juliana and Wobus, Christiane E
Journal of visualized experiments : JoVE, ISSN 1940-087X, 2012, Issue 66, pp. e4297 - 6
Murine norovirus (MNV) is the only member of the Norovirus genus that efficiently grows in tissue culture (1, 2). Cell lysis and cytopathic effect (CPE) are... 
Norovirus | Microbiology | Murine norovirus | Virology | Infection | Medicine | Immunology | MNV | Issue 66 | Murine macrophages | Plaque assay | Molecular biology | RAW 264.7 cells | Molecular Biology | MULTIDISCIPLINARY SCIENCES | norovirus | plaque assay | murine norovirus | murine macrophages | Mice | Animals | Macrophages - virology | Norovirus - growth & development | Viral Plaque Assay - methods | Macrophages - cytology
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2. Full Text Enumeration of bacteriophages by double agar overlay plaque assay
by Kropinski, Andrew M and Mazzocco, Amanda and Waddell, Thomas E and Lingohr, Erika and Johnson, Roger P
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2009, Volume 501, pp. 69 - 76
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In... 
Bacteriophages - isolation & purification | Agar | Viral Plaque Assay - methods | Bacteriophages - growth & development | Viral Plaque Assay - instrumentation
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3. Full Text Detection of Murine Norovirus 1 by Using Plaque Assay, Transfection Assay, and Real-Time Reverse Transcription-PCR before and after Heat Exposure
by Leen Baert and Christiane E. Wobus and Els Van Coillie and Larissa B. Thackray and Johan Debevere and Mieke Uyttendaele
Applied and Environmental Microbiology, ISSN 0099-2240, 01/2008, Volume 74, Issue 2, pp. 543 - 546
Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit... 
INACTIVATION | FELINE CALICIVIRUS | MODEL SYSTEM | VIRUS | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | RT-PCR | INFECTIVITY | MICROBIOLOGY | CELL-CULTURE | SHELLFISH | HYBRIDIZATION | WATER | Temperature | Caliciviridae Infections - virology | Gastroenteritis - virology | Hot Temperature | Animals | Norovirus - growth & development | RNA, Viral - genetics | Transfection - methods | Viral Plaque Assay - methods | Reverse Transcriptase Polymerase Chain Reaction - methods | Mice | RNA, Viral - metabolism | Norovirus - genetics | Polymerase chain reaction | Usage | Norovirus | Analysis | Genetic aspects | Research | Genetic transcription | Methods
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4. Full Text Development and application of a higher throughput RSV plaque assay by immunofluorescent imaging
by Wen, Zhiyun and Citron, Mike and Bett, Andrew J and Espeseth, Amy S and Vora, Kalpit A and Zhang, Lan and DiStefano, Daniel J
Journal of Virological Methods, ISSN 0166-0934, 01/2019, Volume 263, pp. 88 - 95
Viral plaque assays are important tools in the development and evaluation of new antiviral drugs or vaccines in both preclinical and clinical research. While... 
Automation | RSV | Drug evaluation | Higher throughput plaque assay | VIROLOGY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | BIOCHEMICAL RESEARCH METHODS | RESPIRATORY SYNCYTIAL VIRUS | Optical Imaging | Reproducibility of Results | Sigmodontinae - immunology | Humans | Respiratory Syncytial Viruses - immunology | Drug Evaluation | Neutralization Tests | Antibodies, Viral - blood | Respiratory Syncytial Virus Infections - immunology | Viral Vaccines - administration & dosage | Algorithms | Animals | Viral Plaque Assay - methods | Respiratory Syncytial Viruses - growth & development | Sigmodontinae - virology | Cell Line, Tumor | Female | Respiratory Syncytial Virus Infections - virology | Viral Vaccines - immunology | Antibodies, Neutralizing - blood | Disease Models, Animal | High-Throughput Screening Assays - methods | Viral antibodies | Antiviral agents | Medical research | Communicable diseases | Medicine, Experimental | Antibodies | Health aspects
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5. Full Text New low-viscosity overlay medium for viral plaque assays
by Matrosovich, Mikhail and Matrosovich, Tatyana and Garten, Wolfgang and Klenk, Hans-Dieter
Virology Journal, ISSN 1743-422X, 08/2006, Volume 3, Issue 1, pp. 63 - 63
Background: Plaque assays in cell culture monolayers under solid or semisolid overlay media are commonly used for quantification of viruses and antiviral... 
VIROLOGY | Influenza A virus - growth & development | Viscosity | Cell Line | Methylcellulose | Animals | Viral Plaque Assay - methods | Humans | Cellulose | Dogs | Agar | Culture Media - chemistry | Influenza A virus - classification
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6. Full Text Infectious viral quantification of chikungunya virus—virus plaque assay
by Kaur, Parveen and Lee, Regina Ching Hua and Chu, Justin Jang Hann
Methods in Molecular Biology, ISSN 1064-3745, 2016, Volume 1426, pp. 93 - 103
The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous... 
Carboxymethyl cellulose | Virus titration | Crystal violet | Aquacide | Plaque assays | Chikungunya virus | Cell Line | Animals | Viral Plaque Assay - methods | Chikungunya virus - growth & development | Viral Load - methods
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7. Full Text Development and optimization of a direct plaque assay for trypsin-dependent human metapneumovirus strains
by Xu, Jiuyang and Zhang, Yu and Williams, John V
Journal of Virological Methods, ISSN 0166-0934, 09/2018, Volume 259, pp. 1 - 9
Human metapneumovirus (HMPV) is a non-segmented, negative strand RNA virus belonging to the family , previously a subfamily of . It is a leading cause of lower... 
Quantification | Plaque assay | Human metapneumovirus | CELLS | TRACT DISEASE | BIOCHEMICAL RESEARCH METHODS | PNEUMONIA REQUIRING HOSPITALIZATION | CHILDREN | PATHOGENESIS | NEUTRALIZATION ASSAY | CLONED CDNA | RECOVERY | VIROLOGY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | INFECTION | RESPIRATORY SYNCYTIAL VIRUS | Viral Plaque Assay - methods | Metapneumovirus - growth & development | Paramyxoviridae Infections - virology | Trypsin - metabolism | Viral Load - methods | Humans | Viral antibodies | Medical colleges | Trypsin | Antibodies | Respiratory tract diseases | Children | Diseases
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8. Full Text Assessment of plaque assay methods for alphaviruses
by Juarez, Diana and Long, Kanya C and Aguilar, Patricia and Kochel, Tadeusz J and Halsey, Eric S
Journal of Virological Methods, ISSN 0166-0934, 01/2013, Volume 187, Issue 1, pp. 185 - 189
â–º Three plaque assay methods were evaluated for alphavirus detection. â–º Mayaro virus, VEE virus, Una virus, and WEE virus were tested. â–º The solid method... 
Alphavirus | Plaque assay | Carboxymethylcellulose | Agarose | BIOCHEMICAL RESEARCH METHODS | AGAR | REGION | DENGUE | TRANSMISSION | VIROLOGY | MAYARO-VIRUS | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PERU | VENEZUELAN-EQUINE-ENCEPHALITIS | REDUCTION NEUTRALIZATION | Alphavirus Infections - diagnosis | Cricetinae | Alphavirus - immunology | Humans | Cercopithecus aethiops | Neutralization Tests | Antibodies, Viral - blood | Alphavirus Infections - virology | Animals | Encephalitis Virus, Western Equine - immunology | Viral Plaque Assay - methods | Encephalitis Virus, Venezuelan Equine - immunology | Mice | Vero Cells | Antibodies, Neutralizing - blood
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9. Full Text A rapid, simple, and accurate plaque assay for human respiratory syncytial virus (HRSV)
by Kim, Kyung Sook and Kim, Ah-Ra and Piao, Ying and Lee, Ju-Hie and Quan, Fu-Shi
Journal of Immunological Methods, ISSN 0022-1759, 07/2017, Volume 446, pp. 15 - 20
Plaque assays of human respiratory syncytial virus (HRSV) are time-consuming, requiring 4 to 7 days for plaque formation and several hours for dye staining.... 
Human respiratory syncytial virus | Immunostaining | Plaque assay | PROTECTION | PARTICLES | BIOCHEMICAL RESEARCH METHODS | IMMUNOLOGY | BALB/C MICE | PATHOGENESIS | RESPONSES | RSV | FUSION PROTEIN | STRAIN | CELL | Immunohistochemistry - methods | Viral Plaque Assay - methods | Time Factors | Respiratory Syncytial Virus, Human - immunology | Humans | Respiratory Syncytial Virus, Human - isolation & purification | Hep G2 Cells | Monoclonal antibodies | Medicine, Experimental | Medical colleges | Medical research | Health aspects | Biomedical engineering
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10. Full Text Simplified dengue virus microwell plaque assay using an automated quantification program
by Boonyasuppayakorn, Siwaporn and Suroengrit, Aphinya and Srivarangkul, Pimsiri and Yuttithamnon, Wanchalerm and Pankaew, Saran and Saelee, Thanaphon and Prompetchara, Eakachai and Salakij, Saran and Bhattarakosol, Parvapan
Journal of Virological Methods, ISSN 0166-0934, 11/2016, Volume 237, pp. 25 - 31
The plaque assay is essential for virion quantitation but the classic protocol requires considerable efforts. A simplified dengue 96-well plaque assay with... 
VIROLOGY | RNA | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | DRUG DISCOVERY | COMPOUND | BIOCHEMICAL RESEARCH METHODS | REPLICON SYSTEM | INFECTION | INHIBITORS | FLAVIVIRUSES | IDENTIFICATION | Cell Line | Viral Plaque Assay - methods | Data Accuracy | Humans | Dengue Virus - physiology | Dengue Virus - isolation & purification | Dengue - virology | Automation, Laboratory - methods | Dengue - diagnosis | Viral Plaque Assay - instrumentation | Mechanical engineering | Dengue viruses | Antiviral agents | Drug discovery
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11. Full Text Comparison of the plaque assay and 50% tissue culture infectious dose assay as methods for measuring filovirus infectivity
by Smither, Sophie J and Lear-Rooney, Calli and Biggins, Julia and Pettitt, Jamie and Lever, Mark S and Olinger, Gene G
Journal of Virological Methods, ISSN 0166-0934, 11/2013, Volume 193, Issue 2, pp. 565 - 571
Two common methods of quantifying filovirus infectivity, a plaque assay and 50% cell culture infectious dose (TCID ) endpoint dilution assay, were compared.... 
Tissue culture infectious dose | Filoviruses | Counting | Assays | Plaque | SURVIVAL | VIRUS | BIOCHEMICAL RESEARCH METHODS | EBOLA | PATHOGENESIS | AEROSOL | VIROLOGY | HEMORRHAGIC-FEVER | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | Filoviridae - growth & development | Reproducibility of Results | Animals | Viral Plaque Assay - methods | Sensitivity and Specificity | Cercopithecus aethiops | Microbial Viability | Filoviridae - physiology | Viral Load - methods | Vero Cells | Virus Cultivation - methods
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12. Full Text Viral concentration determination through plaque assays: Using traditional and novel overlay systems
by Baer, Alan and Kehn-Hall, Kylene
Journal of Visualized Experiments, ISSN 1940-087X, 11/2014, Issue 93, p. e52065
Plaque assays remain one of the most accurate methods for the direct quantification of infectious virons and antiviral substances through the counting of... 
Viral quantification | Influenza | Venezuelan equine encephalitis | Crystal violet staining | Plaque assay | Cellular overlays | Serial dilutions | Avicel | Agarose | Rift valley fever virus | Issue 93 | Virology | Rift Valley fever virus | CANINE KIDNEY-CELLS | VERO | MULTIDISCIPLINARY SCIENCES | Crystal Violet Staining | REPLICATION | Venezuelan Equine Encephalitis | Cellular Overlays | ESTABLISHED LINE | Viral Quantification | INFLUENZA-VIRUS | MDCK | Plaque Assay | Serial Dilutions | Viruses - isolation & purification | Carboxymethylcellulose Sodium | Animals | Viral Plaque Assay - methods | Humans | Cellulose
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13. Full Text Quantification of RSV infectious particles by plaque assay and immunostaining assay
by Jorquera, Patricia A and Tripp, Ralph A
Methods in Molecular Biology, ISSN 1064-3745, 2016, Volume 1442, pp. 33 - 40
One of the most commonly used approaches for determining the quantity of infectious RSV particles in a given sample is the plaque assay. RSV infectious... 
HEp-2 cells | RSV | VERO cells | F protein | Plaque assay | Immunostaining | G protein | Cell Line, Tumor - virology | Immunoassay | Animals | Antibodies, Viral - metabolism | Vero Cells - virology | Viral Plaque Assay - methods | Virus Replication | Humans | Respiratory Syncytial Viruses - growth & development | Respiratory Syncytial Viruses - immunology | Cercopithecus aethiops | Respiratory Syncytial Viruses - pathogenicity
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14. Full Text Detection of zika virus in desiccated mosquitoes by real-time reverse transcription PCR and plaque assay
by Burkhalter, Kristen L and Savage, Harry M
Emerging Infectious Diseases, ISSN 1080-6040, 04/2017, Volume 23, Issue 4, pp. 680 - 681
We assayed Zika virus-infected mosquitoes stored at room temperature for <30 days for live virus by using plaque assay and virus RNA by using real-time reverse... 
Aedes - virology | Animals | Viral Plaque Assay - methods | Desiccation | Humans | Reverse Transcriptase Polymerase Chain Reaction - methods | Real-Time Polymerase Chain Reaction - methods | Cercopithecus aethiops | Female | Zika Virus - isolation & purification | RNA, Viral - isolation & purification | Vero Cells | INFECTIOUS DISEASES | RNA | HOLDING CONDITIONS | POOLS | IMMUNOLOGY | VENEZUELAN EQUINE ENCEPHALITIS | WEST-NILE | Polymerase chain reaction | Mosquitoes | Usage | Research | Genetic transcription | mosquitoes | viruses | Dispatch | Zika virus | Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay | virus detection | virus persistence in mosquitoes | plaque assay | arboviruses | Culicidae | cold chain | virus RNA | real-time reverse transcription PCR | Vero cells | Zika viruses
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15. Full Text Detection of viable murine norovirus using the plaque assay and propidium-monoazide-combined real-time reverse transcription-polymerase chain reaction
by Lee, Minhwa and Seo, Jina and Seo, Dong Joo and Oh, Hyejin and Jeon, Su Been and Ha, Sang-Do and Myoung, Jinjong and Choi, Changsun and Choi, In-Soo
Journal of Virological Methods, ISSN 0166-0934, 09/2015, Volume 221, pp. 57 - 61
Human norovirus (HuNoV) is the most common cause of gastroenteritis worldwide. The lack of a virus culture system makes it difficult to determine the viability... 
Viable | Propidium monoazide | Plaque assay | Murine norovirus | Ethidium monoazide | VIRUS | BIOCHEMICAL RESEARCH METHODS | VIROLOGY | HEPATITIS | DISCRIMINATION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | EXTRACTION METHODS | INFECTION | DIFFERENTIATION | PCR | WATER | Norovirus - physiology | Cell Line | Temperature | Norovirus - isolation & purification | Propidium - metabolism | Real-Time Polymerase Chain Reaction - methods | Microbial Viability | Virology - methods | Animals | Macrophages - virology | Viral Plaque Assay - methods | Propidium - analogs & derivatives | Reverse Transcriptase Polymerase Chain Reaction - methods | Mice | Azides - metabolism | Polymerase chain reaction | Genetic transcription | RNA | Zoonoses
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16. Full Text A double layer plaque assay using spread plate technique for enumeration of bacteriophage MS2
by Cormier, Jiemin and Janes, Marlene
Journal of Virological Methods, ISSN 0166-0934, 02/2014, Volume 196, pp. 86 - 92
Bacteriophage MS2 is used widely as a model organism to estimate pathogenic virus survival in various environments, and is usually quantified by plaque assay.... 
Plaque assay | Plaque size | Bacteriophage MS2 | Spread plate | Clarity | Consistency | Levivirus - isolation & purification | Reproducibility of Results | Viral Plaque Assay - methods | Culture Media - chemistry | Viral Load - methods | TRANSFORMATION | PENTOSE-PHOSPHATE PATHWAY | COLIPHAGES | ESCHERICHIA-COLI | BIOCHEMICAL RESEARCH METHODS | MS2 | INACTIVATION | FELINE CALICIVIRUS | VIROLOGY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | DISINFECTION | TRANSCRIPTION-PCR ASSAYS | WATER SAMPLES
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17. Full Text Immunostained plaque assay for detection and titration of rabies virus infectivity
by Park, Jun-Sun and Um, Jihye and Choi, Young-Ki and Lee, Yeong Seon and Ju, Young Ran and Kim, Su Yeon
Journal of Virological Methods, ISSN 0166-0934, 02/2016, Volume 228, pp. 21 - 25
The fluorescent antibody test (FAT) is the most commonly used method for detection of the rabies virus (RV). The plaque assay can only be applied to fixed RVs,... 
Plaque assay | Detection | Rabies virus | Immunostaining | PATHOGENICITY | VIROLOGY | CER CELLS | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | SEROGROUP VIRUSES | BIOCHEMICAL RESEARCH METHODS | MICE | GLYCOPROTEIN | Microbial Sensitivity Tests | Rabies virus - isolation & purification | Rabies virus - pathogenicity | Animals | Viral Plaque Assay - methods | Immunoassay - methods | Mice | Rabies - diagnosis | Rabies virus - growth & development | Neutralization Tests - methods | Antibodies, Viral - blood | Rabies | Zoonoses
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18. Full Text Enumeration of bacteriophages using the small drop plaque assay system
by Mazzocco, Amanda and Waddell, Thomas E and Lingohr, Erika and Johnson, Roger P
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2009, Volume 501, pp. 81 - 85
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology.... 
Bacteriophages - isolation & purification | Bacteriophages - growth & development | Viral Plaque Assay - methods
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19. Full Text Survival of adenovirus types 2 and 41 in surface and ground waters measured by a plaque assay
by Rigotto, C and Hanley, K and Rochelle, P.A and De Leon, R and Barardi, C.R.M and Yates, M.V
Environmental Science and Technology, ISSN 0013-936X, 05/2011, Volume 45, Issue 9, pp. 4145 - 4150
To manage artificial recharge systems, it is necessary to understand the inactivation process of microorganisms within aquifers so that requirements regarding... 
RIVER WATER | ENTEROVIRUSES | ENVIRONMENTAL SCIENCES | ENGINEERING, ENVIRONMENTAL | SWIMMING POOL | CELL CULTURE | COASTAL | OUTBREAK | TAP WATER | CARCINOMA | NESTED PCR PROCEDURE | VIRUSES | Adenoviridae Infections - virology | Fresh Water - virology | Temperature | Humans | Cell Line, Tumor | Viral Plaque Assay | Adenoviridae - physiology | Water Supply | Water Microbiology
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20. Full Text Standardization of the filovirus plaque assay for use in preclinical studies
by Shurtleff, Amy C and Biggins, Julia E and Keeney, Ashley E and Zumbrun, Elizabeth E and Bloomfield, Holly A and Kuehne, Ana and Audet, Jennifer L and Alfson, Kendra J and Griffiths, Anthony and Olinger, Gene G and Bavari, Sina and Assay Working Grp and Grp FANG and Filovirus Animal Nonclinical Group (FANG) Assay Working Group
Viruses, ISSN 1999-4915, 2012, Volume 4, Issue 12, pp. 3511 - 3530
The filovirus plaque assay serves as the assay of choice to measure infectious virus in a cell culture, blood, or homogenized tissue sample. It has been in use... 
Ebolavirus | Marburg virus | Vero | Marburgvirus | Filovirus | Plaque assay | Ebola | NONHUMAN-PRIMATES | marburgvirus | POSTEXPOSURE PROTECTION | GUINEA-PIGS | filovirus | MARBURG VIRUSES | CHALLENGE | ebolavirus | VIROLOGY | plaque assay | EBOLA-VIRUS | Viral Plaque Assay - standards | Marburgvirus - growth & development | Animals | Viral Plaque Assay - methods | Marburgvirus - isolation & purification | Cercopithecus aethiops | Viral Load - standards | Ebolavirus - growth & development | Viral Load - methods | Vero Cells | Ebolavirus - isolation & purification
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