Journal of Chromatography A, ISSN 0021-9673, 2006, Volume 1101, Issue 1, pp. 1 - 24
Affinity chromatography has the reputation of a more expensive and less robust than other types of liquid chromatography. Furthermore, the technique is...
Affinity ligand | Microprotein scaffold | Rational design | Enzyme purification | Affinity chromatography | Protein purification | Dye-ligand | Proteomics | Ligand design | Bioinformatics | Triazine scaffold | Combinatorial library | rational design | affinity chromatography | microprotein scaffold | CHEMISTRY, ANALYTICAL | NEUTROPHIL ELASTASE INHIBITORS | proteomics | BETA-GALACTOSE DEHYDROGENASE | BIOCHEMICAL RESEARCH METHODS | protein purification | DNA-BINDING PROTEINS | triazine scaffold | RAY CRYSTAL-STRUCTURE | BOIDINII FORMATE DEHYDROGENASE | dye-ligand | enzyme purification | BIOMIMETIC DYE-LIGANDS | CIBACRON BLUE F3GA | BACTERIAL RECEPTOR DOMAIN | affinity ligand | bioinformatics | MITOCHONDRIAL MALATE-DEHYDROGENASE | LIVER ALCOHOL-DEHYDROGENASE | ligand design | combinatorial library | Blood Coagulation Factors - isolation & purification | Peptide Library | Proteins - isolation & purification | Glucose Oxidase - isolation & purification | Kallikreins - isolation & purification | Galactose Dehydrogenases - isolation & purification | Glycoproteins - isolation & purification | Prions - isolation & purification | Triazines - chemistry | Pancreatic Elastase - isolation & purification | Chromatography, Affinity - methods | Protein Structure, Tertiary | alpha 1-Antitrypsin - isolation & purification | Computational Biology - methods | Staphylococcal Protein A - isolation & purification | L-Lactate Dehydrogenase - isolation & purification | Aldehyde Oxidoreductases - isolation & purification | DNA-Binding Proteins - isolation & purification | Combinatorial Chemistry Techniques | SELEX Aptamer Technique | Ligands | Antibodies - isolation & purification | Bacterial Proteins - isolation & purification | Proinsulin - isolation & purification | Coloring Agents - chemistry | Peptides | Enzymes | Liquid chromatography
Affinity ligand | Microprotein scaffold | Rational design | Enzyme purification | Affinity chromatography | Protein purification | Dye-ligand | Proteomics | Ligand design | Bioinformatics | Triazine scaffold | Combinatorial library | rational design | affinity chromatography | microprotein scaffold | CHEMISTRY, ANALYTICAL | NEUTROPHIL ELASTASE INHIBITORS | proteomics | BETA-GALACTOSE DEHYDROGENASE | BIOCHEMICAL RESEARCH METHODS | protein purification | DNA-BINDING PROTEINS | triazine scaffold | RAY CRYSTAL-STRUCTURE | BOIDINII FORMATE DEHYDROGENASE | dye-ligand | enzyme purification | BIOMIMETIC DYE-LIGANDS | CIBACRON BLUE F3GA | BACTERIAL RECEPTOR DOMAIN | affinity ligand | bioinformatics | MITOCHONDRIAL MALATE-DEHYDROGENASE | LIVER ALCOHOL-DEHYDROGENASE | ligand design | combinatorial library | Blood Coagulation Factors - isolation & purification | Peptide Library | Proteins - isolation & purification | Glucose Oxidase - isolation & purification | Kallikreins - isolation & purification | Galactose Dehydrogenases - isolation & purification | Glycoproteins - isolation & purification | Prions - isolation & purification | Triazines - chemistry | Pancreatic Elastase - isolation & purification | Chromatography, Affinity - methods | Protein Structure, Tertiary | alpha 1-Antitrypsin - isolation & purification | Computational Biology - methods | Staphylococcal Protein A - isolation & purification | L-Lactate Dehydrogenase - isolation & purification | Aldehyde Oxidoreductases - isolation & purification | DNA-Binding Proteins - isolation & purification | Combinatorial Chemistry Techniques | SELEX Aptamer Technique | Ligands | Antibodies - isolation & purification | Bacterial Proteins - isolation & purification | Proinsulin - isolation & purification | Coloring Agents - chemistry | Peptides | Enzymes | Liquid chromatography
Journal Article
Biotechnology Advances, ISSN 0734-9750, 09/2015, Volume 33, Issue 5, pp. 435 - 456
In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of...
Multimeric enzymes | IMAC | Controlled immobilization | Chimeric proteins | Covalent attachment | Enzyme stabilization | Ionic exchange | PENICILLIN-G ACYLASE | MULTIPOINT COVALENT ATTACHMENT | REVERSIBLE IMMOBILIZATION | SITE-DIRECTED MUTAGENESIS | SALT-INDUCED IMMOBILIZATION | PANCREATIC RIBONUCLEASE-A | SELECTIVE ADSORPTION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GLYOXYL-AGAROSE | ION AFFINITY-CHROMATOGRAPHY | CHITIN-BINDING DOMAIN | Biotechnology | Enzymes, Immobilized - chemistry | Enzymes, Immobilized - metabolism | Chromatography, Ion Exchange | Proteins - metabolism | Enzyme Stability | Enzymes, Immobilized - isolation & purification | Proteins - chemistry | Proteins - isolation & purification | Enzymes | Viral antibodies | Antibodies | Index Medicus | Covalence | Immobilization | Strategy | Affinity | Joining | Silicates
Multimeric enzymes | IMAC | Controlled immobilization | Chimeric proteins | Covalent attachment | Enzyme stabilization | Ionic exchange | PENICILLIN-G ACYLASE | MULTIPOINT COVALENT ATTACHMENT | REVERSIBLE IMMOBILIZATION | SITE-DIRECTED MUTAGENESIS | SALT-INDUCED IMMOBILIZATION | PANCREATIC RIBONUCLEASE-A | SELECTIVE ADSORPTION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GLYOXYL-AGAROSE | ION AFFINITY-CHROMATOGRAPHY | CHITIN-BINDING DOMAIN | Biotechnology | Enzymes, Immobilized - chemistry | Enzymes, Immobilized - metabolism | Chromatography, Ion Exchange | Proteins - metabolism | Enzyme Stability | Enzymes, Immobilized - isolation & purification | Proteins - chemistry | Proteins - isolation & purification | Enzymes | Viral antibodies | Antibodies | Index Medicus | Covalence | Immobilization | Strategy | Affinity | Joining | Silicates
Journal Article
1998, 2nd., ISBN 0471186260, x, 695
Book
Journal of Chromatography B, ISSN 1570-0232, 2007, Volume 848, Issue 1, pp. 48 - 63
Antibody purification seems to be safely ensconced in a platform, now well-established by way of multiple commercialized antibody processes. However, natural...
Membrane chromatography | PAT | Purification | Protein precipitation | Viral clearance | Protein crystallization | Manufacturing facilities | Antibody | Industrial | Centrifugation | Protein A | Chromatography | Alternatives to protein A | Membrane filtration | Future | Aqueous two-phase separation | Monoclonal | Large scale | Disposable technology | Depth filtration | CHEMISTRY, ANALYTICAL | PROTEIN-A | membrane filtration | protein precipitation | monoclonal | LARGE-SCALE | TRANSGENIC GOAT MILK | manufacturing facilities | protein crystallization | THERAPEUTIC PROTEINS | purification | chromatography | membrane chromatography | protein A | CELL-CULTURE | disposable technology | antibody | BIOCHEMICAL RESEARCH METHODS | alternatives to protein A | A AFFINITY-CHROMATOGRAPHY | centrifugation | large scale | industrial | viral clearance | future | TANGENTIAL FLOW FILTRATION | RECOMBINANT PROTEINS | aqueous two-phase separation | MOVING-BED CHROMATOGRAPHY | depth filtration | MONOCLONAL-ANTIBODIES | Chromatography, Affinity - trends | Protein Engineering - methods | Antibodies - chemistry | Ligands | Centrifugation - methods | Centrifugation - trends | Antibodies - isolation & purification | Forecasting | Protein Engineering - trends | Chromatography, Affinity - methods | Viral antibodies | Antibodies
Membrane chromatography | PAT | Purification | Protein precipitation | Viral clearance | Protein crystallization | Manufacturing facilities | Antibody | Industrial | Centrifugation | Protein A | Chromatography | Alternatives to protein A | Membrane filtration | Future | Aqueous two-phase separation | Monoclonal | Large scale | Disposable technology | Depth filtration | CHEMISTRY, ANALYTICAL | PROTEIN-A | membrane filtration | protein precipitation | monoclonal | LARGE-SCALE | TRANSGENIC GOAT MILK | manufacturing facilities | protein crystallization | THERAPEUTIC PROTEINS | purification | chromatography | membrane chromatography | protein A | CELL-CULTURE | disposable technology | antibody | BIOCHEMICAL RESEARCH METHODS | alternatives to protein A | A AFFINITY-CHROMATOGRAPHY | centrifugation | large scale | industrial | viral clearance | future | TANGENTIAL FLOW FILTRATION | RECOMBINANT PROTEINS | aqueous two-phase separation | MOVING-BED CHROMATOGRAPHY | depth filtration | MONOCLONAL-ANTIBODIES | Chromatography, Affinity - trends | Protein Engineering - methods | Antibodies - chemistry | Ligands | Centrifugation - methods | Centrifugation - trends | Antibodies - isolation & purification | Forecasting | Protein Engineering - trends | Chromatography, Affinity - methods | Viral antibodies | Antibodies
Journal Article
Nature Methods, ISSN 1548-7091, 2013, Volume 10, Issue 8, pp. 730 - +
Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any...
INTERACTION NETWORK | COMPLEX | TANDEM | TRANSCRIPTION MACHINERY REVEALS | PROTEIN-INTERACTION PARTNERS | KINASE | BIOCHEMICAL RESEARCH METHODS | IDENTIFICATION | STATISTICAL-MODEL | Protein Interaction Mapping - methods | Humans | Proteins - analysis | Proteomics - methods | Mass Spectrometry - methods | Chromatography, Affinity - methods | Databases, Factual | Technology application | Usage | Records and correspondence | Mass spectrometry | Proteomics | Proteins | Peptides | Kinases | Bioinformatics | Open source software
INTERACTION NETWORK | COMPLEX | TANDEM | TRANSCRIPTION MACHINERY REVEALS | PROTEIN-INTERACTION PARTNERS | KINASE | BIOCHEMICAL RESEARCH METHODS | IDENTIFICATION | STATISTICAL-MODEL | Protein Interaction Mapping - methods | Humans | Proteins - analysis | Proteomics - methods | Mass Spectrometry - methods | Chromatography, Affinity - methods | Databases, Factual | Technology application | Usage | Records and correspondence | Mass spectrometry | Proteomics | Proteins | Peptides | Kinases | Bioinformatics | Open source software
Journal Article
Nature Methods, ISSN 1548-7091, 01/2011, Volume 8, Issue 1, pp. 70 - 73
We present 'significance analysis of interactome' (SAINT), a computational tool that assigns confidence scores to protein-protein interaction data generated...
YEAST | LABEL-FREE | PROTEIN-INTERACTION NETWORKS | QUANTITATIVE PROTEOMICS | BIOCHEMICAL RESEARCH METHODS | REVEALS | Protein Interaction Mapping - methods | Proteins - metabolism | Computer Simulation | Mass Spectrometry | Computational Biology | Probability | Protein Binding | Proteins - isolation & purification | Chromatography, Affinity - methods | Usage | Research | Mass spectrometry | Protein-protein interactions | Applications software | Protein binding | Computational mathematics | Biochemistry | Research methodology | Proteomics
YEAST | LABEL-FREE | PROTEIN-INTERACTION NETWORKS | QUANTITATIVE PROTEOMICS | BIOCHEMICAL RESEARCH METHODS | REVEALS | Protein Interaction Mapping - methods | Proteins - metabolism | Computer Simulation | Mass Spectrometry | Computational Biology | Probability | Protein Binding | Proteins - isolation & purification | Chromatography, Affinity - methods | Usage | Research | Mass spectrometry | Protein-protein interactions | Applications software | Protein binding | Computational mathematics | Biochemistry | Research methodology | Proteomics
Journal Article
1990, Methods in enzymology, ISBN 0121820831, Volume 182., xxix, 894
Book
Journal of Chromatography A, ISSN 0021-9673, 2012, Volume 1221, pp. 57 - 70
This article reviews technology trends in antibody purification. Section discusses non-chromatography methods, including precipitation, liquid–liquid...
Non-chromatographic | Chromatographic | Remedial purification | Antibody purification | CHARGE INDUCTION CHROMATOGRAPHY | CHEMISTRY, ANALYTICAL | METAL-AFFINITY-CHROMATOGRAPHY | BIOCHEMICAL RESEARCH METHODS | AQUEOUS 2-PHASE EXTRACTION | HYDROPHOBIC INTERACTION CHROMATOGRAPHY | MIXED-MODE CHROMATOGRAPHY | PROTEIN-A CHROMATOGRAPHY | EXPANDED-BED ADSORPTION | SIZE-EXCLUSION CHROMATOGRAPHY | HUMAN MONOCLONAL-ANTIBODIES | CATION-EXCHANGE CHROMATOGRAPHY | Viral antibodies | Antibodies | Precipitation (Meteorology) | Chromatography
Non-chromatographic | Chromatographic | Remedial purification | Antibody purification | CHARGE INDUCTION CHROMATOGRAPHY | CHEMISTRY, ANALYTICAL | METAL-AFFINITY-CHROMATOGRAPHY | BIOCHEMICAL RESEARCH METHODS | AQUEOUS 2-PHASE EXTRACTION | HYDROPHOBIC INTERACTION CHROMATOGRAPHY | MIXED-MODE CHROMATOGRAPHY | PROTEIN-A CHROMATOGRAPHY | EXPANDED-BED ADSORPTION | SIZE-EXCLUSION CHROMATOGRAPHY | HUMAN MONOCLONAL-ANTIBODIES | CATION-EXCHANGE CHROMATOGRAPHY | Viral antibodies | Antibodies | Precipitation (Meteorology) | Chromatography
Journal Article
1989, ISBN 3527261842, xi, 502
Book
1974, Methods in enzymology, ISBN 0121818977, Volume 34., 810
Book
Methods in Molecular Biology, ISSN 1064-3745, 2019, Volume 1876, pp. 111 - 124
A major hurdle in the studies of nitrogenase, one of the most complicated metalloenzymes known to date, is to obtain large amounts of intact, active proteins....
Anaerobic protein purification | MoFe protein | Nitrogenase | Size-exclusion chromatography (SEC) | Gel filtration | Fe–S reconstitution | Immobilized metal-affinity chromatography (IMAC) | VFe protein | Fe protein | Weak anion-exchange chromatography (WAEC) | Heterologous expression
Anaerobic protein purification | MoFe protein | Nitrogenase | Size-exclusion chromatography (SEC) | Gel filtration | Fe–S reconstitution | Immobilized metal-affinity chromatography (IMAC) | VFe protein | Fe protein | Weak anion-exchange chromatography (WAEC) | Heterologous expression
Journal Article
Journal of Chromatography A, ISSN 0021-9673, 08/2014, Volume 1355, pp. 1 - 14
Research on RNA has led to many important biological discoveries and improvement of therapeutic technologies. From basic to applied research, many procedures...
Amino acids | Purification | RNA | Affinity chromatography | Isolation methods | CHEMISTRY, ANALYTICAL | POTENTIAL APPLICATION | RAPID PURIFICATION | BIOCHEMICAL RESEARCH METHODS | NUCLEIC-ACIDS | PERFORMANCE LIQUID-CHROMATOGRAPHY | LARGE-SCALE PREPARATION | RECOMBINANT RNA | MESSENGER-RNA | RNA AFFINITY TAGS | CLINICAL-APPLICATIONS | SUPERCOILED PLASMID DNA | Histidine | Ribonucleic acids | Chemical engineering | Ligands | Biological | Chromatography
Amino acids | Purification | RNA | Affinity chromatography | Isolation methods | CHEMISTRY, ANALYTICAL | POTENTIAL APPLICATION | RAPID PURIFICATION | BIOCHEMICAL RESEARCH METHODS | NUCLEIC-ACIDS | PERFORMANCE LIQUID-CHROMATOGRAPHY | LARGE-SCALE PREPARATION | RECOMBINANT RNA | MESSENGER-RNA | RNA AFFINITY TAGS | CLINICAL-APPLICATIONS | SUPERCOILED PLASMID DNA | Histidine | Ribonucleic acids | Chemical engineering | Ligands | Biological | Chromatography
Journal Article