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Journal Article
Journal Article
Journal Article
Amino Acids, ISSN 0939-4451, 05/2013, Volume 44, Issue 5, pp. 1329 - 1336
Journal Article
Protein Science, ISSN 0961-8368, 06/2004, Volume 13, Issue 6, pp. 1489 - 1502
7‐Azatryptophan (AW), a noncoded isostere of tryptophan (W), possesses interesting spectral properties. In particular, the presence of a nitrogen atom at... 
FPLC, fast protein liquid chromatography | PEG, polyethylene glycol | thrombin | HM2, hirudin variant isolated from the leech Hirudinaria manillensis | UV, ultraviolet | HOBt, 1‐hydroxybenzotriazole | folding | TEA, triethylamine | ESI, electrospray ionization | β‐ME, β‐mercaptoethanol | a.m.u., atomic mass units | Y3W, synthetic analog of the hirudin HM2 fragment 1–47 in which Tyr 3 has been replaced by tryptophan | AW, (L)‐7‐azatryptophan | Cha, cyclohexylalanine | DTT, dithiothreitol | Fmoc, 9‐fluorenyl‐methyl‐oxycarbonyl | spectroscopy | Y3AW, synthetic analog of the hirudin HM2 fragment 1–47 in which Tyr 3 has been replaced by L‐7‐azatryptophan | NMR, nuclear magnetic resonance | anticoagulants | Fmoc‐ONSu, 9‐fluorenyl‐methyloxycarbonyl‐O‐N‐hydroxy‐succinimide | HBTU, 2–(1H‐benzotriazol‐1‐yl‐)‐1,1,3,3‐tetrameth‐yluronium hexafluorophosphate | EDT, ethanedithiol | TOF, time of flight | FT‐IR, Fourier transformed infrared | W, (L)‐tryptophan | BSA, bovine serum albumin | 7AI, 7‐azaindole | noncoded amino acids | RP, reverse‐phase | ChCl, choline chloride | FLEC, (+)‐1–(9‐fluorenyl)ethylchloroformate | MES, morpholinoethanesulfonic acid | 7‐azatryptophan | hirudin | HPLC, high‐pressure liquid chromatography | CD, circular dichroism | TFA, trifluoroacetic acid | Anticoagulants | Spectroscopy | Noncoded amino acids | 7-azatryptophan | Thrombin | Folding | Hirudin | EXCITED-STATE TAUTOMERIZATION | ACTIVE-SITE | PROTEIN-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | NUCLEAR-MAGNETIC-RESONANCE | BIOSYNTHETIC INCORPORATION | HUMAN ALPHA-THROMBIN | CHEMICAL-SYNTHESIS | STAPHYLOCOCCAL NUCLEASE | AFFINITY-CHROMATOGRAPHY | TRYPTOPHAN ANALOGS | Hirudins - metabolism | Amino Acid Sequence | Hirudins - chemistry | Models, Molecular | Molecular Sequence Data | Spectrometry, Fluorescence | Chromatography, High Pressure Liquid | Tryptophan - metabolism | Protein Folding | Leeches | Molecular Probes - analysis | Thermodynamics | Animals | Molecular Probes - metabolism | Tryptophan - analogs & derivatives | Protein Binding | Thrombin - metabolism | Tryptophan - analysis
Journal Article
Journal Article
Journal Article