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Protein Expression and Purification, ISSN 1046-5928, 11/2016, Volume 127, pp. 88 - 97
An immunogenic protein, enolase 2, was identified among the secreted excretory/secretory antigens (ESAs) from strain RH using immunoproteomics based on... 
Excretory/secretory antigens | Enolase 2 | Toxoplasma gondii | Enzyme activity | Immunoproteomic | ANTIBODIES | LOCALIZATION | ALPHA-ENOLASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | EXCRETED-SECRETED ANTIGENS | MAINTENANCE | INVASION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PLASMINOGEN-BINDING-PROTEIN | MOLECULAR-CLONING | PROTECTIVE ANTIGEN | LEISHMANIA | Toxoplasma - immunology | Antigens, Protozoan - immunology | Antigens, Protozoan - biosynthesis | Protozoan Proteins - pharmacology | Recombinant Proteins - biosynthesis | Toxoplasma - enzymology | Recombinant Proteins - isolation & purification | Antigens, Protozoan - pharmacology | Escherichia coli - metabolism | Antigens, Protozoan - isolation & purification | Phosphopyruvate Hydratase - isolation & purification | Immunization | Antibodies, Monoclonal, Murine-Derived - immunology | Antibodies, Protozoan - immunology | Recombinant Proteins - pharmacology | Phosphopyruvate Hydratase - immunology | Protozoan Proteins - biosynthesis | Phosphopyruvate Hydratase - biosynthesis | Animals | Recombinant Proteins - immunology | Phosphopyruvate Hydratase - pharmacology | Protozoan Proteins - isolation & purification | Mice | Mice, Inbred BALB C | Protozoan Proteins - immunology | Antigens | Enzymes | Immune response | Ionization | Analysis | Monoclonal antibodies | Mass spectrometry | Physiological aspects
Journal Article
Journal Article
Journal Article
PLoS ONE, ISSN 1932-6203, 11/2012, Volume 7, Issue 11, p. e49780
In the last decade, the search for new vaccines against canine visceral leishmaniasis has intensified. However, the pattern related to immune protection during... 
REAL-TIME PCR | TISSUE PARASITE DENSITY | NATURALLY INFECTED-DOGS | IMMUNE-RESPONSE | NITRIC-OXIDE PRODUCTION | LONG-LASTING PROTECTION | MULTIDISCIPLINARY SCIENCES | CANINE VISCERAL LEISHMANIASIS | SAND FLY SALIVA | CLINICAL STATUS | EXCRETED-SECRETED ANTIGENS | T-Lymphocyte Subsets - immunology | Antigens, Protozoan - immunology | Leishmaniasis Vaccines - immunology | Immunoglobulin G - blood | Male | Monocytes - metabolism | Monocytes - immunology | Antigens, CD - metabolism | Antibodies, Protozoan - blood | Leishmaniasis, Visceral - veterinary | Lymphocyte Activation - immunology | Immunity, Humoral | Dog Diseases - immunology | Immunoglobulin G - immunology | Female | Histocompatibility Antigens Class II - metabolism | Leishmania infantum - genetics | B-Lymphocytes - metabolism | Dog Diseases - parasitology | Dog Diseases - prevention & control | Cytokines - immunology | Cytokines - metabolism | Adjuvants, Immunologic | Antibodies, Protozoan - immunology | Parasite Load | Immunophenotyping | Saponins - immunology | Animals | B-Lymphocytes - immunology | Histocompatibility Antigens Class II - immunology | T-Lymphocyte Subsets - metabolism | Dogs | Leishmania infantum - immunology | Antigens | Medical research | Immunoglobulin G | Vaccination | Vaccines | B cells | Leishmaniasis | Transforming growth factors | T cells | Infection | Medicine, Experimental | Health aspects | Cell proliferation | CD8 antigen | Infections | Lymphocytes T | Parasites | Proteins | Parasitic diseases | Lymphocytes | Visceral leishmaniasis | Deoxyribonucleic acid--DNA | Spleen | Vector-borne diseases | Evaluation | Immunization | Cytokines | Salivary gland | CD4 antigen | Experimental infection | Immunogenicity | Lymphocytes B | γ-Interferon | Interleukin 10 | Interferon | Saliva | Deoxyribonucleic acid | DNA
Journal Article
Journal Article
PLoS Neglected Tropical Diseases, ISSN 1935-2727, 2016, Volume 10, Issue 5, p. e0004614
Preventive vaccination is a highly promising strategy for interrupting leishmaniasis transmission that can, additionally, contribute to elimination. A vaccine... 
REAL-TIME PCR | CUTANEOUS LEISHMANIASIS | FML-VACCINE | CANINE VISCERAL LEISHMANIASIS | DOUBLE-BLIND | GENE FAMILY | INFANTUM | INFECTION | EXCRETED-SECRETED ANTIGENS | PARASITOLOGY | TROPICAL MEDICINE | LIESP/QA-21 VACCINE | Antigens, Protozoan - immunology | Leishmaniasis Vaccines - immunology | Leishmaniasis, Visceral - prevention & control | Immunoglobulin G - blood | Leishmaniasis, Visceral - immunology | Protozoan Proteins - genetics | Th1 Cells - immunology | Leishmania infantum - physiology | Antibodies, Protozoan - blood | Leishmaniasis, Visceral - veterinary | Dog Diseases - immunology | Polymerase Chain Reaction | Female | Antigens, Protozoan - genetics | Leishmaniasis, Visceral - parasitology | Leishmania mexicana - immunology | Dog Diseases - parasitology | Dog Diseases - prevention & control | Macrophages - immunology | Disease Models, Animal | Adaptive Immunity | Leishmania mexicana - chemistry | Nitric Oxide - biosynthesis | Adjuvants, Immunologic | Parasite Load | Antigens, Surface - genetics | Leishmaniasis Vaccines - administration & dosage | Recombinant Proteins - administration & dosage | Bone Marrow - parasitology | Animals | Recombinant Proteins - immunology | Dogs | Leishmania infantum - immunology | Leishmaniasis Vaccines - genetics | Antigens, Surface - immunology | Immunity, Cellular | Leishmania mexicana - genetics | Interferon-gamma - biosynthesis | Protozoan Proteins - immunology | Antigens | Immune response | Leishmania | Genetic aspects | Genetic recombination | Health aspects | Parasites | Vaccines | Parasitic diseases | Tropical diseases | Life Sciences | Vaccinology | Immunology
Journal Article
Vaccine, ISSN 0264-410X, 2007, Volume 25, Issue 21, pp. 4301 - 4311
Abstract To investigate the vaccine potential of both the Toxoplasma GRA2 and GRA6 antigens, the full length recombinant proteins were produced in Escherichia... 
Allergy and Immunology | Protozoa | GRA6 | Toxoplasma gondii | Dense granule | GRA2 | Vaccination | MEDICINE, RESEARCH & EXPERIMENTAL | PARASITOPHOROUS VACUOLE | ESCHERICHIA-COLI | IMMUNOGLOBULIN-G ANTIBODIES | IMMUNOLOGY | CONGENITAL TOXOPLASMOSIS | EXCRETED-SECRETED ANTIGENS | protozoa | GONDII INFECTION | dense granule | INTERFERON-GAMMA | PREGNANT-WOMEN | VETERINARY SCIENCES | CIRCULATING ANTIGEN | DNA VACCINATION | vaccination | Toxoplasma - immunology | Antigens, Protozoan - immunology | Vaccines, Subunit - isolation & purification | Vaccines, Subunit - genetics | Humans | Lipid A - analogs & derivatives | Vaccines, Synthetic - immunology | Protozoan Proteins - genetics | Th1 Cells - immunology | Toxoplasmosis, Animal - prevention & control | Vaccines, Synthetic - isolation & purification | Antibodies, Protozoan - blood | Lipid A - immunology | Mice, Inbred CBA | Antigens, Protozoan - isolation & purification | Antigens, Protozoan - genetics | Brain - parasitology | Cytokines - immunology | Disease Models, Animal | Protozoan Vaccines - immunology | Adjuvants, Immunologic | Vaccines, Synthetic - genetics | Vaccines, Subunit - immunology | Animals | Escherichia coli - genetics | Protozoan Proteins - isolation & purification | Toxoplasmosis, Animal - immunology | Mice | Protozoan Proteins - immunology | Antigens | Analysis | Immunoglobulin G | Lipids | Health aspects | Recombinant proteins | Medical research | Parasites | Vaccines | Immune system | Toxoplasmosis, Animal | Brain | Vaccines, Subunit | Cytokines | Escherichia coli | Biochemistry, Molecular Biology | Antibodies, Protozoan | Protozoan Proteins | Lipid A | Toxoplasma | Antigens, Protozoan | Life Sciences | Th1 Cells | Protozoan Vaccines | Vaccines, Synthetic
Journal Article
PLoS ONE, ISSN 1932-6203, 11/2015, Volume 10, Issue 11, p. e0143063
Leishmania parasites and some molecules they secrete are known to modulate innate immune responses through effects on dendritic cells (DCs) and macrophages.... 
TYROSINE-PHOSPHATASE SHP-1 | CUTANEOUS LEISHMANIASIS | PROTECTIVE IMMUNITY | HOST IMMUNE-RESPONSE | MAJOR INFECTION | FACTOR-ALPHA PRODUCTION | MULTIDISCIPLINARY SCIENCES | EXCRETED-SECRETED ANTIGENS | NF-KAPPA-B | VISCERAL LEISHMANIASIS | T-CELLS | Humans | Protozoan Proteins - pharmacology | Down-Regulation - drug effects | Antigens, CD - metabolism | Up-Regulation - drug effects | Cell Differentiation - drug effects | Lipopolysaccharides - pharmacology | Dendritic Cells - drug effects | Interleukin-10 - biosynthesis | Cell Membrane - metabolism | Dendritic Cells - cytology | HLA-DR Antigens - immunology | Leishmania infantum - metabolism | Tumor Necrosis Factor-alpha - biosynthesis | Cell Membrane - drug effects | Dendritic Cells - metabolism | Immunologic Factors - pharmacology | Interferon-gamma - pharmacology | Interleukin-12 - biosynthesis | Physiological aspects | Causes of | Genetic aspects | Research | Leishmaniasis | Dendritic cells | CD40 antigen | CD86 antigen | Lymphocytes T | Parasites | Kinases | Macrophages | Lipopolysaccharides | Proteins | Cell activation | Parasitic diseases | Rodents | Elongation | Recombinant | Vector-borne diseases | Immune response | Cell survival | Maturation | Cytokines | Immunomodulation | Cultures | Interleukin 12 | CD80 antigen | Inflammation | T cell receptors | Tumor necrosis factor-α | Costimulator | Monocytes | Overexpression | γ-Interferon | Interleukin 10 | Interferon | Histocompatibility antigen HLA | Differentiation | Adenosine triphosphatase | Life Sciences
Journal Article