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1. Full Text High-precision isothermal titration calorimetry with automated peak-shape analysis
by Keller, Sandro and Vargas, Carolyn and Zhao, Huaying and Piszczek, Grzegorz and Brautigam, Chad A and Schuck, Peter
Analytical Chemistry, ISSN 0003-2700, 06/2012, Volume 84, Issue 11, pp. 5066 - 5073
Isothermal titration calorimetry (ITC) is a powerful classical method that enables researchers in many fields to study the thermodynamics of molecular... 
CHEMISTRY, ANALYTICAL | SOLUBILIZATION | COMPLEX | COOPERATIVITY | RECONSTITUTION | Carbonic Anhydrase II - analysis | Mesylates - analysis | Thermodynamics | Animals | Cattle | Flow Injection Analysis - statistics & numerical data | Least-Squares Analysis | Hot Temperature | Automation, Laboratory | Calorimetry - methods | Measurement | Usage | Isothermal titration calorimetry | Analysis | Enthalpy | Decomposition (Chemistry) | Chemical reactions | Parameter estimation | Molecular chemistry | Stochastic models | Index Medicus | protein interactions | isothermal titration microcalorimetry | binding enthalpy | singular value decomposition
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2. Full Text Revisiting the enzymatic kinetics of pepsin using isothermal titration calorimetry
by Luo, Qi and Chen, Dongxin and Boom, Remko M and Janssen, Anja E.M
Food Chemistry, ISSN 0308-8146, 12/2018, Volume 268, pp. 94 - 100
Pepsin is the first protease that food proteins encounter in the digestive tract. However, most of the previous studies on the enzymatic kinetics of pepsin... 
Bovine serum albumin | Pepsin | Isothermal titration calorimetry | Enzymatic kinetics | Gastric digestion | OPA method | MECHANISM | FOOD SCIENCE & TECHNOLOGY | WHEY-PROTEIN | HYDROLYSIS | DIGESTION | IN-VITRO | NUTRITION & DIETETICS | CHEMISTRY, APPLIED | IONIC-STRENGTH | BINDING | DIGESTIBILITY | FOOD | Hydrolysis | Kinetics | Pepsin A - metabolism | Peptides | Pepsin A - pharmacokinetics | Calorimetry - methods | Albumin | Calorimetry | Proteases | Proteolysis | Index Medicus | Levensmiddelenproceskunde | VLAG | Leerstoelgroep Levensmiddelenproceskunde | Food Process Engineering
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3. Full Text Enzyme kinetics in crowded solutions from isothermal titration calorimetry
by Maximova, Ksenia and Wojtczak, Jakub and Trylska, Joanna
Analytical Biochemistry, ISSN 0003-2697, 02/2019, Volume 567, pp. 96 - 105
Isothermal titration calorimetry (ITC) is a universal technique that directly measures the heat absorbed or released in a process. ITC is typically used to... 
Macromolecular crowding | Trypsin | Isothermal titration calorimetry | Enzyme kinetics | CHEMISTRY, ANALYTICAL | ASSAY | BIOCHEMISTRY & MOLECULAR BIOLOGY | ALPHA-CHYMOTRYPSIN | BIOCHEMICAL RESEARCH METHODS | PARAMETERS | TRYPSIN-CATALYZED HYDROLYSIS
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4. Full Text Characterizing Drug-Polymer Bead Interactions Using Isothermal Titration Calorimetry
by Swaine, Tanya S and Garcia, Pedro and Tang, Yiqing and Lewis, Andrew L and Parkes, Gareth and Waters, Laura J
Journal of Pharmaceutical Sciences, ISSN 0022-3549, 2019, Volume 108, Issue 5, pp. 1772 - 1778
Isothermal titration calorimetry was used to investigate thermodynamic and kinetic binding interactions between 4 clinically relevant drugs: doxorubicin (Dox),... 
polymer | drug-eluting beads | stoichiometry | binding | isothermal titration calorimetry | doxorubicin | CHEMISTRY, MEDICINAL | CHEMOEMBOLIZATION | ELUTING BEADS | EFFICACY | RELEASE | CHEMISTRY, MULTIDISCIPLINARY | DELIVERY | IN-VITRO EVALUATION | DC BEAD | IRINOTECAN | PHARMACOLOGY & PHARMACY | POLYETHYLENE-GLYCOL
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5. Full Text Bayesian analysis of isothermal titration calorimetry for binding thermodynamics
by Nguyen, Trung Hai and Rustenburg, Ariën S and Krimmer, Stefan G and Zhang, Hexi and Clark, John D and Novick, Paul A and Branson, Kim and Pande, Vijay S and Chodera, John D and Minh, David D.L
PLoS ONE, ISSN 1932-6203, 09/2018, Volume 13, Issue 9, pp. e0203224 - e0203224
Isothermal titration calorimetry (ITC) is the only technique able to determine both the enthalpy and entropy of noncovalent association in a single experiment.... 
RAPID MEASUREMENT | DESIGN | LIGAND-BINDING | RNA | RECOGNITION | MULTIDISCIPLINARY SCIENCES | STATISTICAL ERROR | INHIBITOR BINDING | ASSOCIATION | LEAST-SQUARES | PROTONATION | Bayesian statistical decision theory | Thermodynamics | Usage | Enthalpy | Analysis | Isothermal titration calorimetry | Research | Binding | Uncertainty | Data analysis | Laboratories | Titration calorimetry | Data processing | Entropy | Decomposition | Biology | Heat measurement | Regression analysis | Proteins | Standard data | Calorimetry | Ligands | Titration | Bayesian analysis | Binding sites | Index Medicus
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6. Full Text Critique of methods for estimating heats in isothermal titration calorimetry
by Tellinghuisen, Joel
Analytical Biochemistry, ISSN 0003-2697, 12/2018, Volume 563, pp. 79 - 86
Isothermal titration calorimetry data recorded on a MicroCal/Malvern VP-ITC instrument for water-water blanks and for dilution of aqueous solutions of BaCl and... 
ITC | Variance function analysis | Isothermal titration calorimetry | Estimation precision | Weighted least squares | CALIBRATION | CHEMISTRY, ANALYTICAL | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | BIOCHEMICAL REACTIONS | PARAMETERS | LEAST-SQUARES | INSTRUMENT | STATISTICAL ERROR | VANT HOFF | Least-Squares Analysis | Thermodynamics | Temperature | Algorithms | Hot Temperature | Calorimetry - methods | Executives | Analysis | Calorimetry | Noise control | Methods
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7. Full Text Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
by Mazzei, Luca and Ciurli, Stefano and Zambelli, Barbara
Methods in Enzymology, ISSN 0076-6879, 2016, Volume 567, pp. 215 - 236
Isothermal titration calorimetry (ITC) is a technique that measures the heat released or absorbed during a chemical reaction as an intrinsic probe to... 
Enzymatic catalysis | Lactate dehydrogenase | Urease | Isothermal titration calorimetry | Enzymatic inhibition | LACTATE-DEHYDROGENASE | CHEMISTRY, ANALYTICAL | INHIBITION | KINETICS | NI2 | SUBSTRATE | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | CHEMISTRY, PHYSICAL | BINDING | L-Lactate Dehydrogenase - metabolism | Urease - metabolism | Catalysis | Kinetics | Calorimetry - methods | Index Medicus
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8. Full Text Measuring Enzymatic Stability by Isothermal Titration Calorimetry
by Chan, WKD and Mason, M and Hansen, LD and Kenealey, JD
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN 1940-087X, 03/2019, Issue 145
This work demonstrates a new method for measuring the stability of enzyme activity by isothermal titration calorimetry (ITC). The peak heat rate observed after... 
Food Technology | Investigative Techniques | MULTIDISCIPLINARY SCIENCES | Enzyme Assays | Industry | Food Industry | isothermal titration calorimetry | Technology | invertase | Analytical | and Agriculture | differential scanning calorimetry | enzyme activity | Issue 145 | enzyme immobilization | Chemistry Techniques | Calorimetry | Diagnostic and Therapeutic Techniques and Equipment | Agriculture | lactase | Environmental Sciences
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9. Full Text Isothermal titration calorimetry in a polymeric microdevice
by Jia, Yuan and Zhang, Zhixing and Su, Chao and Lin, Qiao
Microfluidics and Nanofluidics, ISSN 1613-4982, 05/2017, Volume 21, Issue 5, p. 1
Microelectromechanical systems (MEMS) technology can enable the integration of isothermal titration calorimetry (ITC) on a single chip for direct thermodynamic... 
Isothermal titration calorimetry (ITC) | Biomolecular reactions systems | Polymeric microdevice | Thermodynamic characterization | INSTRUMENTS & INSTRUMENTATION | CONSTANTS | PHYSICS, FLUIDS & PLASMAS | NANOSCIENCE & NANOTECHNOLOGY | Microelectromechanical systems | Fabrication | Receptors | Thermal power | Solutions | Ligands | Calorimetry | Thermal conductivity | Titration | Polymers | Reliability
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10. Full Text Measuring Rapid Time-Scale Reaction Kinetics Using Isothermal Titration Calorimetry
by Di Trani, Justin M and Moitessier, Nicolas and Mittermaier, Anthony K
Analytical Chemistry, ISSN 0003-2700, 07/2017, Volume 89, Issue 13, pp. 7022 - 7030
Isothermal titration calorimetry (ITC) is a powerful tool for acquiring both thermodynamic and kinetic data for biological interactions including molecular... 
PROLYL OLIGOPEPTIDASE | CHEMISTRY, ANALYTICAL | CONSTANTS | ENZYME-KINETICS | MICROCALORIMETRY | MECHANISM | COMPENSATION | HYDROLYSIS | PARAMETERS | INHIBITORS | BINDING | Molecular dynamics | Usage | Trypsin | Research | Isothermal titration calorimetry | Chemical reaction, Rate of | Measurement | Enzymes | Balances (scales) | Complications | Measuring instruments | Prolyl oligopeptidase | Titration calorimetry | Heat flow | Time measurement | Heat measurement | Experiments | Empirical analysis | Oligopeptidase | Reaction kinetics | Calorimetry | Titration | Heat transmission | Kinetics | Catalysis | Heat transfer
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11. Full Text Isothermal titration calorimetry of membrane proteins — Progress and challenges
by Rajarathnam, Krishna and Rösgen, Jörg
BBA - Biomembranes, ISSN 0005-2736, 01/2014, Volume 1838, Issue 1, pp. 69 - 77
Integral membrane proteins, including G protein-coupled receptors (GPCR) and ion channels, mediate diverse biological functions that are crucial to all aspects... 
Thermodynamics | Isothermal titration calorimetry (ITC) | Affinity | Drug discovery | Enthalpy | Membrane proteins | LIGAND-BINDING | RECOGNITION | MECHANISM | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | METAL-BINDING | CXCR1 RECEPTOR | INTERLEUKIN-8 | TRANSPORTER YIIP | BIOPHYSICS | DISSOCIATION | Ligands | Membrane Proteins - chemistry | Detergents - chemistry | Calorimetry - methods | Calorimetry | G proteins | Protein binding
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12. Full Text Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro
by Lin, Kui and Lin, Kui and Wu, Geng and Wu, Geng
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2019, Volume 1893, pp. 257 - 272
In the study of the Hippo signal transduction pathway, protein-protein interactions are often explored, because various proteins such as MOB1, NDR1/NDR2, and... 
Association constant | Dissociation constant | ITC | Binding affinity | Isothermal titration calorimetry | Protein–protein interaction | Models, Theoretical | Thermodynamics | Algorithms | Proteins - metabolism | Protein Binding | Calorimetry | Proteins - chemistry | In Vitro Techniques
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13. Full Text Isothermal Titration Calorimetry Measurements of Metal Ions Binding to Proteins
by Quinn, Colette F and Carpenter, Margaret C and Croteau, Molly L and Wilcox, Dean E
Methods in Enzymology, ISSN 0076-6879, 2016, Volume 567, pp. 3 - 21
ITC measurements involving metal ions are susceptible to a number of competing reactions (oxidation, precipitation, and hydrolysis) and coupled reactions... 
Proteins | Binding enthalpy | Buffer competition | Chelation titration | Isothermal titration calorimetry | Metal ions | CHEMISTRY, ANALYTICAL | COPPER(II) BINDING | CONSTANTS | ZN2+ BINDING | THERMODYNAMIC ANALYSIS | BIOCHEMISTRY & MOLECULAR BIOLOGY | CARBONIC-ANHYDRASE II | COMPLEXES | BIOCHEMICAL RESEARCH METHODS | CHEMISTRY, PHYSICAL | COORDINATION PROPERTIES | FERRIC IONS | GLUTATHIONE | SITES | Protein Binding | Calorimetry | Metals - metabolism | Proteins - metabolism | Index Medicus
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14. Full Text Characterization of Membrane Protein Interactions by Isothermal Titration Calorimetry
by Situ, Alan J and Schmidt, Thomas and Mazumder, Parichita and Ulmer, Tobias S
Journal of Molecular Biology, ISSN 0022-2836, 10/2014, Volume 426, Issue 21, pp. 3670 - 3680
Understanding the structure, folding, and interaction of membrane proteins requires experimental tools to quantify the association of transmembrane (TM)... 
protein–protein interaction | transmembrane association | membrane proteins | isothermal titration calorimetry | NMR spectroscopy | protein-protein interaction | HELIX-HELIX INTERACTIONS | STABILITY | BIOCHEMISTRY & MOLECULAR BIOLOGY | ENERGETICS | DIMERIZATION | MICELLES | LIPID-BILAYERS | PHOSPHORUS NMR | FREE-ENERGY | TRANSMEMBRANE HELIX | PHOSPHOLIPID BICELLES | Protein Structure, Tertiary | Temperature | Magnetic Resonance Spectroscopy | Protein Structure, Secondary | Humans | Phospholipids - chemistry | Protein Folding | Calorimetry - methods | Membranes - metabolism | Thermodynamics | Lipids - chemistry | Membrane Proteins - chemistry | Protein Binding | Lipid Bilayers - chemistry | Binding Sites | Platelet Glycoprotein GPIIb-IIIa Complex - chemistry | Calorimetry | Nuclear magnetic resonance spectroscopy | Chemical properties | Membrane proteins | Integrins | Index Medicus
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15. Full Text Kinetics of trypsin-catalyzed hydrolysis determined by isothermal titration calorimetry
by Maximova, Ksenia and Trylska, Joanna
Analytical Biochemistry, ISSN 0003-2697, 10/2015, Volume 486, pp. 24 - 34
Isothermal titration calorimetry (ITC) was applied to determine enzymatic activity and inhibition. We measured the Michaelis–Menten kinetics for... 
Benzamidine | Trypsin | Isothermal titration calorimetry | Casein | Enzyme kinetics | α1-Antitrypsin | SYSTEM | CHEMISTRY, ANALYTICAL | CONSTANTS | MECHANISM | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | PARAMETERS | alpha 1-Antitrypsin | INHIBITION | ENZYME-KINETICS | BINDING | Biocatalysis | Trypsin Inhibitors - pharmacology | Models, Molecular | Benzamidines - pharmacology | Trypsin - metabolism | Calorimetry - methods | Hydrolysis | Thermodynamics | Animals | Cattle | Phenylmethylsulfonyl Fluoride - pharmacology | alpha 1-Antitrypsin - pharmacology | Protein Conformation | Kinetics | Trypsin - chemistry | Calorimetry | Index Medicus
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16. Full Text Ion binding to transport proteins using isothermal titration calorimetry
by Liu, Shian and Lockless, Steve W
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1684, pp. 289 - 303
Isothermal titration calorimetry (ITC) is an emerging, label-free technology used to measure ligand binding to membrane proteins. This technology utilizes a... 
Isothermal titration calorimetry | Ion binding | Equilibrium | Transporter | Channel | Protein Binding | Thermodynamics | Calorimetry | Carrier Proteins - metabolism | Hot Temperature | Ions - metabolism | Index Medicus
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17. Full Text A microfabrication-based approach to quantitative isothermal titration calorimetry
by Wang, Bin and Jia, Yuan and Lin, Qiao
Biosensors and Bioelectronics, ISSN 0956-5663, 04/2016, Volume 78, pp. 438 - 446
Isothermal titration calorimetry (ITC) directly measures heat evolved in a chemical reaction to determine equilibrium binding properties of biomolecular... 
Differential calorimetry | Isothermal titration calorimetry | Binding isotherm | Microfabrication | Biomolecular interaction | ELECTROCHEMISTRY | CALIBRATION | CHEMISTRY, ANALYTICAL | CONSTANTS | DRUG DESIGN | SENSOR | NANOSCIENCE & NANOTECHNOLOGY | BIOMOLECULES | MICROCALORIMETER | THERMODYNAMICS | BIOPHYSICS | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | THERMAL CHARACTERIZATION | Thermodynamics | Cytidine - chemistry | Barium Compounds - chemistry | Crown Ethers - chemistry | Biosensing Techniques | Protein Binding | Calorimetry | Ligands | Chlorides - chemistry | Ribonuclease, Pancreatic - chemistry | Microtechnology | Crown ethers | Analysis | Ribonuclease | Microfluidics | Biomedical engineering | Yuan (China) | Index Medicus | Binding | Titration calorimetry | Biomolecules | Devices | Design analysis | Thermoelectricity
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18. Full Text Thermodynamic study of complexation of Zn(II)/L (L− = acetate, indomethacin and diclofenac anions) by isothermal titration calorimetry
by Rodríguez-Laguna, Norma and Reyes-García, Luis Ignacio and Pacheco-Gómez, Raúl and Flores, Raúl and Rojas-Hernández, Alberto and Gómez-Balderas, Rodolfo
Journal of Thermal Analysis and Calorimetry, ISSN 1388-6150, 5/2019, Volume 136, Issue 4, pp. 1701 - 1709
In this work, a thermodynamic study of the Zn(II)/L systems (L− = acetate, AcO− or indomethacin anion, Indo− or diclofenac anion, Dic−) in ethanolic solution... 
Polymer Sciences | Thermodynamic formation constants | Chemistry | Analytical Chemistry | Measurement Science and Instrumentation | Physical Chemistry | Zn(II)-diclofenac | Zn(II)-indomethacin | Inorganic Chemistry | Isothermal titration calorimetry | Zn(II)-NSAIDs | CHEMISTRY, ANALYTICAL | CONSTANTS | CU(II) | CHEMISTRY, PHYSICAL | COPPER | INHIBITION | THERMODYNAMICS | NONSTEROIDAL ANTIINFLAMMATORY DRUGS | TENOXICAM | METAL-COMPLEXES | Thermal properties | Thermodynamics | Calorimetry | Indomethacin | Analysis | Stoichiometry | Complexation | Anions | Enthalpy | Titration calorimetry | Ions | Titration | Nonsteroidal anti-inflammatory drugs | Heat measurement | Thermodynamic properties | Functional groups | Diclofenac
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19. Full Text Thermochemical Measurements of Cation Exchange in CdSe Nanocrystals Using Isothermal Titration Calorimetry
by Jharimune, Suprita and Sathe, Ajay A and Rioux, Robert M
Nano Letters, ISSN 1530-6984, 11/2018, Volume 18, Issue 11, pp. 6795 - 6803
Among the various reported post synthetic modifications of colloidal nanocrystals, cation exchange (CE) is one of the most promising and versatile approaches... 
thermodynamics | isothermal titration calorimetry | nanocrystal size | capping ligands | CdSe nanocrystals | cation exchange | PHYSICS, CONDENSED MATTER | ENERGY | PHYSICS, APPLIED | LIGAND-BINDING | EQUILIBRIUM-CONSTANTS | COMPENSATION | MATERIALS SCIENCE, MULTIDISCIPLINARY | CHEMISTRY, PHYSICAL | NANOSCIENCE & NANOTECHNOLOGY | CHEMISTRY, MULTIDISCIPLINARY | ENTHALPY | OXIDE NANOPARTICLES | ENTROPY TITRATION | GROWTH | SURFACE
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