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Toxicology in Vitro, ISSN 0887-2333, 2011, Volume 25, Issue 1, pp. 257 - 266
A stable luciferase reporter gene assay was developed based on the thyroid hormone responsive rat pituitary tumor GH3 cell line that constitutively expresses... 
Endocrine disruption | PCB metabolite | Bisphenol A | Amiodarone | Thyroid hormone receptor | PBDE | TRANSCRIPTIONAL ACTIVITY | ENDOCRINE DISRUPTOR | ENVIRONMENTAL CHEMICALS | TETRABROMOBISPHENOL-A | CULTURED GH1 CELLS | PITUITARY-TUMOR-CELLS | GROWTH-HORMONE | BROMINATED FLAME RETARDANTS | TOXICOLOGY | HALOGENATED AROMATIC-HYDROCARBONS | POLYCHLORINATED-BIPHENYLS | Microchemistry - methods | Cell Count | Receptors, Thyroid Hormone - antagonists & inhibitors | Endocrine Disruptors - pharmacology | Receptors, Thyroid Hormone - genetics | Transfection | Triiodothyronine - metabolism | Toxicity Tests - methods | Genes, Reporter | High-Throughput Screening Assays - methods | Luciferases, Firefly - metabolism | Cell Survival - drug effects | Limit of Detection | Reproducibility of Results | Response Elements | Environmental Pollutants - pharmacology | Rats | Endocrine Disruptors - analysis | Receptors, Thyroid Hormone - metabolism | Animals | Receptors, Thyroid Hormone - agonists | Plasmids | Thyroxine - metabolism | Cell Line, Tumor | Luciferases, Firefly - genetics | Environmental Pollutants - analysis | Index Medicus | Acetic acid | growth-hormone | pituitary-tumor-cells | cultured gh1 cells | transcriptional activity | brominated flame retardants | tetrabromobisphenol-a | halogenated aromatic-hydrocarbons | polychlorinated-biphenyls | endocrine disruptor | environmental chemicals
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2019, Volume 1943, pp. 227 - 239
Investigation of nanoparticle-mediated nucleic acid delivery is a key step for the development of nucleic acid-based nanotherapeutics. Using luciferases as... 
Gene delivery | Lipoplex nanoparticles | In vitro transfection | Nucleic acid delivery | Polyplex nanoparticles | Recombinant firefly luciferase | BCA assay | Firefly luciferase assay buffer | Luminescence assay | Index Medicus
Journal Article
Methods in Cell Biology, ISSN 0091-679X, 2017, Volume 142, pp. 89 - 99
Journal Article
Journal of Immunological Methods, ISSN 0022-1759, 10/2011, Volume 373, Issue 1-2, pp. 229 - 239
A cell-based assay has been developed for the quantification of the activity of TNFα antagonists based on human erythroleukemic K562 cells transfected with a... 
TNF-alpha antagonists | Crohn's disease | Neutralizing antibodies | Cell-based assays | Rheumatoid arthritis | Reporter-gene | CYTOKINES | BIOCHEMICAL RESEARCH METHODS | IMMUNOLOGY | BIOAVAILABILITY | BIOASSAY | TUMOR-NECROSIS-FACTOR | IMMUNOGENICITY | THERAPEUTIC PROTEINS | BIOLOGICAL-ACTIVITY | INTERFERONS | INFLIXIMAB | ETANERCEPT | Antibodies, Neutralizing - analysis | Gene Expression Regulation, Enzymologic - drug effects | Luciferases - metabolism | Luciferases, Renilla - genetics | Arthritis, Rheumatoid - blood | Humans | Recombinant Fusion Proteins - metabolism | Luciferases - genetics | Antibodies, Monoclonal - blood | Dose-Response Relationship, Drug | Serum - immunology | Antibodies, Neutralizing - immunology | Infliximab | Luciferases, Renilla - metabolism | Arthritis, Rheumatoid - drug therapy | Antibodies, Monoclonal, Humanized - blood | Antibodies, Monoclonal, Humanized - pharmacology | Tumor Necrosis Factor-alpha - immunology | Culture Media - chemistry | Antibodies, Monoclonal - immunology | Luciferases, Firefly - metabolism | Antibodies, Monoclonal - pharmacology | Regulatory Sequences, Nucleic Acid - genetics | Tumor Necrosis Factor-alpha - pharmacology | Animals | NF-kappa B - genetics | Crohn Disease - blood | Crohn Disease - drug therapy | Culture Media - pharmacology | K562 Cells | Recombinant Fusion Proteins - genetics | Luciferases, Firefly - genetics | Adalimumab | Antibodies, Monoclonal, Humanized - immunology | Antibodies, Neutralizing - blood | Tumor Necrosis Factor-alpha - antagonists & inhibitors | Serum - chemistry | Index Medicus
Journal Article
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2009, Volume 485, pp. 395 - 405
Neutralizing antibody (NAb) assays for human immunodeficiency virus (HIV) are used to study the immune response in infected individuals, to examine monoclonal... 
Reporter gene | HIV vaccines | Neutralizing antibodies | Assay standardization | Pseudovirus | Cell Line | Animals | Luciferases - metabolism | Neutralization Tests - standards | HIV Antibodies - immunology | Humans | HIV - immunology | Neutralization Tests - methods | Genes, Reporter | Index Medicus
Conference Proceeding
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2009, Volume 486, pp. 109 - 123
Journal Article
Journal Article
Conference Proceeding
by Wu, LH and Zhang, WY and Qiu, X and Wang, CR and Liu, YF and Wang, ZW and Yu, Y and Ye, RD and Zhang, Y
MOLECULES, ISSN 1420-3049, 10/2018, Volume 23, Issue 10
Corydalis yanhusuo W. T. Wang (C. yanhusuo) has been traditionally used for drug addiction and pain relief in China. In our previous study, we showed that the... 
QUATERNARY ALKALOIDS | Corydalis yanhusuoW. T. Wang | ACTIVATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | luciferase reporter gene assay | PURIFICATION | DEHYDROCORYDALINE | D-1 receptor | FLIPR | ANTINOCICEPTION | CHEMISTRY, MULTIDISCIPLINARY
Journal Article
Journal Article
Chemosphere, ISSN 0045-6535, 2007, Volume 66, Issue 3, pp. 474 - 479
Many pesticides possess hormonal activity and have thus been classified as endocrine disruptors. Pyrethroids are commonly used pesticides worldwide, but little... 
Fenvalerate | Endocrine disruptor | Permethrin | Cypermethrin | Androgen receptor | 3-Phenoxybenzoic acid | FACTORY-WORKERS | cypermethrin | fenvalerate | SPERMATOZOA | ENVIRONMENTAL ESTROGENS | ENVIRONMENTAL SCIENCES | androgen receptor | 3-phenoxybenzoic acid | permethrin | CHEMICALS | endocrine disruptor | IN-VITRO ASSAYS | WILDLIFE | INSECTICIDES | Luciferases - metabolism | Nitriles - pharmacology | Humans | Imidazolidines - metabolism | Imidazolidines - pharmacology | Permethrin - metabolism | Pyrethrins - pharmacology | Recombinant Fusion Proteins - metabolism | Flutamide - metabolism | Luciferases - genetics | Flutamide - chemistry | Dexamethasone - pharmacology | Transfection | Dihydrotestosterone - pharmacology | Imidazolidines - chemistry | Molecular Structure | Response Elements - genetics | Pesticides - metabolism | Pyrethrins - metabolism | Cell Line | Cell Survival - drug effects | Nitriles - metabolism | Permethrin - chemistry | Permethrin - pharmacology | Androgen Antagonists - pharmacology | Flutamide - pharmacology | Pyrethrins - chemistry | Animals | Receptors, Androgen - genetics | Nitriles - chemistry | Pesticides - chemistry | Recombinant Fusion Proteins - genetics | Cell Proliferation - drug effects | Pesticides - pharmacology | Genetic research | Micropollutants | Insecticidal plants | Hormones, Sex | Flutamide | Chemicals | Index Medicus
Journal Article
Journal Article