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Journal of Microbiological Methods, ISSN 0167-7012, 03/2019, Volume 158, pp. 86 - 92
The introduction of chromosomal mutations into the genome using λRed-mediated recombineering includes two consecutive steps—the insertion of an antibiotic... 
Counterselection | Recombineering | λRed-mediated genome engineering
Journal Article
BioTechniques, ISSN 0736-6205, 09/2018, Volume 65, Issue 3, pp. 159 - 162
A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In ,... 
SecA1 | tetracycline promoter | ClpP | recombineering | promoter replacement
Journal Article
Virology, ISSN 0042-6822, 03/2019, Volume 529, pp. 186 - 194
Human Cytomegalovirus (HCMV) is an opportunistic pathogen that causes substantial disease in neonates and immunocompromised individuals. Reverse genetic... 
CRISPR | Cytomegalovirus | Cas9 | HCMV | Recombineering | VIROLOGY | HOMOLOGY-DIRECTED REPAIR | INFECTION | EFFICIENCY
Journal Article
Progress in Biophysics and Molecular Biology, ISSN 0079-6107, 10/2019, Volume 147, pp. 33 - 46
While much of this volume focuses on mammalian DNA repair systems that are directly involved in genome stability and cancer, it is important to still be... 
Single-stranded DNA binding protein | Exonuclease | Single-strand annealing | DNA recombination | Homologous recombination | Recombineering
Journal Article
JOURNAL OF MICROBIOLOGICAL METHODS, ISSN 0167-7012, 03/2019, Volume 158, pp. 86 - 92
The introduction of chromosomal mutations into the E. coli genome using lambda Red-mediated recombineering includes two consecutive steps-the insertion of an... 
Counterselection | SYSTEM | RECOMBINATION | REPAIR | lambda Red-mediated genome engineering | BIOCHEMICAL RESEARCH METHODS | MICROBIOLOGY | Recombineering
Journal Article
Microbial Cell Factories, ISSN 1475-2859, 12/2019, Volume 18, Issue 1, pp. 1 - 15
Abstract Background Terpenoids are of high interest as chemical building blocks and pharmaceuticals. In microbes, terpenoids can be synthesized via the... 
MEP pathway | Metabolic control analysis | Recombineering | Isoprene | E. coli
Journal Article
Journal of Biological Chemistry, ISSN 0021-9258, 05/2010, Volume 285, Issue 20, pp. 15599 - 15610
CYP24A1 expression is up-regulated by 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) via a vitamin D receptor (VDR)/retinoid X receptor (RXR) heterodimer that... 
Gene Regulation | Transcription Coactivators | Transcription Enhancers | Signal Transduction | Histone Modification | Vitamin D | ChIP-seq | BAC Recombineering | ChIP-chip | Chromatin Immunoprecipitation (ChIP)
Journal Article
Journal Article
Journal Article
by Xin, YP and Mu, YL and Kong, J and Guo, TT
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, ISSN 0099-2240, 05/2019, Volume 85, Issue 9
Lactobacillus casei is a potential cell factory for the production of enzymes and bioactive molecules using episomal plasmids, which suffer from genetic... 
SYSTEM | repetitive integration | Lactobacillus casei | ANTIBIOTIC-RESISTANCE | MICROBIOLOGY | MODEL | targeted chromosomal location | DELETION | POSITION | recombineering system | Cre/loxP system | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | CONSTRUCTION | LACTOCOCCUS-LACTIS
Journal Article
Biotechnology Reports, ISSN 2215-017X, 12/2015, Volume 8, Issue C, pp. 24 - 35
The genetic modification of primary bacterial disease isolates is challenging due to the lack of highly efficient genetic tools. Herein we describe the... 
Bacterial pathogens | Recombineering | Genetic modification
Journal Article