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Methods in Molecular Biology, ISSN 1064-3745, 2014, Volume 1149, pp. 185 - 191
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2017, Volume 1615, pp. 59 - 64
Protein function is generally dependent on its subcellular localisation. In Gram-negative bacteria such as Escherichia coli, a protein can be targeted to five... 
Freeze and thaw | Subcellular localisation | Osmotic shock | Peptidoglycan | Protein solubilisation | Spheroplast | Membrane | Bacterial Proteins - metabolism | Gram-Negative Bacteria - chemistry | Escherichia coli - chemistry | Bacterial Proteins - chemistry | Cell Fractionation - methods | Solubility
Journal Article
JOURNAL OF PROTEOME RESEARCH, ISSN 1535-3893, 01/2020, Volume 19, Issue 1, pp. 75 - 84
We found that nuclear envelopes stabilize against surfactants in the presence of ethylene glycol (EG). We, therefore, developed a novel subcellular... 
phosphoproteomics | LOCALIZATION | subcellular fractionation | ASSISTED-LASER-DESORPTION/IONIZATION | PHOSPHORYLATION | TISSUE | COMPLEXES | BIOCHEMICAL RESEARCH METHODS | phase transfer surfactant | EXTRACTION | ethylene glycol | ENRICHMENT | CELL | EXPRESSION | CD44 | mass spectrometry-compatible method
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2017, Volume 1468, pp. 1 - 9
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1664, pp. 233 - 243
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2017, Volume 1531, pp. 101 - 109
Journal Article
Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.], 2015, Volume 69, pp. 3.1.1 - 3.1.22
This unit provides both a theoretical and a practical background to all the techniques associated with the application of differential and density gradient... 
density gradient technology | instrumentation | differential centrifugation | Animals | Humans | Cell Fractionation - methods | Centrifugation, Density Gradient - methods | Organelles - metabolism | Subcellular Fractions - metabolism | Triiodobenzoic Acids - chemistry
Journal Article
Book Chapter
Methods in Molecular Biology, ISSN 1064-3745, 02/2007, Volume 357, pp. 73 - 85
Conference Proceeding
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2019, Volume 1941, pp. 201 - 223
Subcellular fractionation methods permit the isolation, purification, and/or enrichment of specific cellular compartments from complex tissue samples.... 
Forward trafficking pathway | Density gradient separation | Differential centrifugation | Triton X-100 solubilization | Subcellular localization
Journal Article
BMC Research Notes, ISSN 1756-0500, 2012, Volume 5, Issue 1, pp. 513 - 513
We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic... 
Subcellular fractionation | Skeletal muscle | Western blotting | Cell Line | Mice | Animals | Muscle, Skeletal - cytology | Subcellular Fractions | Mice, Inbred C57BL | Proteins | Muscles | Analysis | Musculoskeletal system | Cell culture | Health sciences | Fractionation | Mitochondria | Colleges & universities | Protocol | Experiments
Journal Article