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1. Full Text Expression of recombinant antibodies
by Frenzel, André and Hust, Michael and Schirrmann, Thomas
Frontiers in Immunology, ISSN 1664-3224, 2013, Volume 4, pp. 217 - 217
Recombinant antibodies are highly specific detection probes in research, diagnostics, and have emerged over the last two decades as the fastest growing class... 
Fungi | Yeast | Mammalian cell | Insect cells | Recombinant antibody | Procaryotes | Transgenic organisms | procaryotes | mammalian cell | FV-FC FUSIONS | SINGLE-CHAIN ANTIBODY | HUMAN MONOCLONAL-ANTIBODY | transgenic organisms | IMMUNOLOGY | yeast | HIGH-LEVEL EXPRESSION | fungi | VARIABLE-FRAGMENT SCFV | FED-BATCH CULTURE | TRANSIENT GENE-EXPRESSION | NON-HODGKINS-LYMPHOMA | LINKED IMMUNOSORBENT ASSAYS | recombinant antibody | insect cells | PEPTIDYLPROLYL CIS,TRANS-ISOMERASE FKPA | transgenic plants | Transgenic Animals | Escherichia coli | Filamentous fungi
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2. Full Text PEAQ: Versatile expression vectors for easy and quick transient expression of heterologous proteins in plants
by Sainsbury, Frank and Thuenemann, Eva C and Lomonossoff, George P
Plant Biotechnology Journal, ISSN 1467-7644, 09/2009, Volume 7, Issue 7, pp. 682 - 693
Agro-infiltration of leaf tissue with binary vectors harbouring a sequence of interest is a rapid method of expressing proteins in plants. It has recently been... 
GATEWAY | Molecular farming | Binary vector | Cowpea mosaic virus | Transient expression | TRANSFORMATION | ANTIBODIES | SYSTEM | VIRUS | binary vector | VIRAL VECTORS | PLANT SCIENCES | AGROBACTERIUM-TUMEFACIENS | transient expression | molecular farming | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | BIOLOGY | Plants, Genetically Modified - metabolism | Plants, Genetically Modified - genetics | Plants, Genetically Modified - growth & development | 3' Untranslated Regions - genetics | Comovirus - physiology | Comovirus - genetics | Genetic Vectors - physiology | Models, Genetic | Genetic Vectors - genetics | Polypeptides | Histidine | Plants | RNA | Recombinant proteins | Index Medicus
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3. Full Text Expression of the β‐glucosidase gene Pgβglu‐1 underpins natural resistance of white spruce against spruce budworm
by Mageroy, Melissa H and Parent, Geneviève and Germanos, Gaby and Giguère, Isabelle and Delvas, Nathalie and Maaroufi, Halim and Bauce, Éric and Bohlmann, Joerg and Mackay, John J
The Plant Journal, ISSN 0960-7412, 01/2015, Volume 81, Issue 1, pp. 68 - 80
Periodic outbreaks of spruce budworm ( SBW ) affect large areas of ecologically and economically important conifer forests in North America, causing tree... 
transcriptome profiling | glucosyl hydrolase | Picea glauca | insect resistance | conifer forest health | acetophenone biosynthesis | WEEVIL | TRANSIENT EXPRESSION | ARABIDOPSIS-THALIANA | CRYSTAL-STRUCTURE | SUBCELLULAR-LOCALIZATION | PATTERNS | MOUNTAIN PINE-BEETLE | PLANT DEFENSES | PLANT SCIENCES | NORWAY SPRUCE | SUBSTRATE-SPECIFICITY | Protein Structure, Tertiary | Picea - enzymology | Plant Proteins - physiology | Picea - physiology | Models, Molecular | Molecular Sequence Data | Gene Expression Profiling | Herbivory | beta-Glucosidase - chemistry | beta-Glucosidase - physiology | Plant Proteins - genetics | Animals | Plant Proteins - chemistry | beta-Glucosidase - genetics | Gene Expression Regulation, Plant | Picea - genetics | Moths - physiology | beta-Glucosidase - metabolism | Plant Proteins - metabolism | Larva - physiology | Acetophenones - metabolism | Disease Resistance - genetics | Index Medicus | Original
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4. Full Text Over‐expression of secreted proteins from mammalian cell lines
by Dalton, Annamarie C and Barton, William A
Protein Science, ISSN 0961-8368, 05/2014, Volume 23, Issue 5, pp. 517 - 525
Secreted mammalian proteins require the development of robust protein over‐expression systems for crystallographic and biophysical studies of protein function.... 
receptor | protein expression | mammalian cell culture | secreted protein | Protein expression | Secreted protein | Mammalian cell culture | Receptor | TRANSIENT TRANSFECTION | HIGH-LEVEL | SYSTEM | BIOCHEMISTRY & MOLECULAR BIOLOGY | INSECT CELLS | N-GLYCOSYLATION | FUSION PARTNERS | CULTURE | RECOMBINANT PROTEINS | GENE-EXPRESSION | PICHIA-PASTORIS | Recombinant Proteins - metabolism | Cell Line | Up-Regulation | Humans | Baculoviridae - genetics | Recombinant Proteins - genetics | Insecta - genetics | Cell Culture Techniques - methods | Genetic Vectors - genetics | Yeasts - genetics | Animals | Transfection | Insecta - cytology | Proteins | Genetic engineering | Cells | Genes | Rodents | Index Medicus | Reviews
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5. Full Text Expression profile of microRNAs in young stroke patients
by Tan, Kay Sin and Armugam, Arunmozhiarasi and Sepramaniam, Sugunavathi and Lim, Kai Ying and Setyowati, Karolina Dwi and Wang, Chee Woon and Jeyaseelan, Kandiah
PLoS ONE, ISSN 1932-6203, 11/2009, Volume 4, Issue 11, pp. e7689 - e7689
Background: The methods currently available for diagnosis and prognosis of cerebral ischaemia still require further improvements. Micro-RNAs (small non-coding... 
BIOMARKERS | HEART | DISEASES | MULTIDISCIPLINARY SCIENCES | TRANSIENT FOCAL ISCHEMIA | GENE-EXPRESSION | RATS | BRAIN | BLOOD | Biomarkers - metabolism | Humans | Middle Aged | Male | MicroRNAs - biosynthesis | Gene Expression Profiling | Reverse Transcriptase Polymerase Chain Reaction | Brain - metabolism | Stroke - metabolism | MicroRNAs - analysis | Adolescent | Brain - pathology | Adult | Female | Principal Component Analysis | Ischemia - pathology | Cluster Analysis | Prognosis | MicroRNA | Medical examination | Ischemia | Analysis | Atherosclerosis | Development and progression | Stroke patients | Blood | Erythropoiesis | Traumatic brain injury | Heart attacks | Profiling | Disease | Identification methods | Insulin-like growth factors | Biochemistry | Adipocytes | Angiogenesis | Cell growth | Coding | Rodents | Peripheral blood | Physiology | Bioindicators | Diagnosis | Heart failure | Stroke | Diabetes mellitus | MiRNA | Principal components analysis | Gene expression | Patients | Medicine | Ribonucleic acids | MicroRNAs | Arteriosclerosis | Biomarkers | Hypoxia | Insulin resistance | Diabetes | Cancer | Veins & arteries | Index Medicus
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6. Full Text A high‐throughput transient expression system for rice
by Page, Mike T and Parry, Martin A.J and Carmo‐Silva, Elizabete
Plant, Cell & Environment, ISSN 0140-7791, 07/2019, Volume 42, Issue 7, pp. 2057 - 2064
Rice is an important global crop and represents a vital source of calories for many food insecure regions. Efforts to improve this crop by improving yield,... 
Oryza sativa | synthetic biology | transient expression | confocal microscopy | high‐throughput | Golden Gate | protoplasts | cellular localisation | carboxysome | Rice | high-throughput | TRANSFORMATION | PROTEIN | PLANTA | PLANT SCIENCES | DNA | GENES | Gene expression | Genetic engineering | Climatic changes | Transformation | Nutrient deficiency | Cloning | Modular systems | Crops | Protoplasts | Transgenic plants | Calories | Climate change | Transformations | Transgenes | Protein interaction | Crop yield | Cassettes | Technical Report
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7. Full Text Minocycline and hypothermia for reperfusion injury after focal cerebral ischemia in the rat—Effects on BBB breakdown and MMP expression in the acute and subacute phase
by Nagel, Simon and Su, Ying and Horstmann, Solveig and Heiland, Sabine and Gardner, Humphrey and Koziol, Jim and Martinez-Torres, Francisco J and Wagner, Simone
Brain Research, ISSN 0006-8993, 2007, Volume 1188, Issue 1, pp. 198 - 206
Abstract Reperfusion injury is a complication of recanalization therapies after focal cerebral ischemia. The disruption of the blood–brain barrier (BBB) caused... 
Neurology | MCAO/R | MRI | Focal cerebral ischemia | Minocycline | Metalloproteinase | BBB breakdown | Hypothermia | ARTERY OCCLUSION | metalloproteinase | MILD HYPOTHERMIA | BLOOD-BRAIN-BARRIER | NEUROSCIENCES | DAMAGE | MATRIX METALLOPROTEINASES | IN-VITRO | MATRIX-METALLOPROTEINASE-9 | ACUTE STROKE | minocycline hypothermia | KNOCKOUT | focal cerebral ischemia | TRANSIENT | Neuroprotective Agents - therapeutic use | Infarction, Middle Cerebral Artery - physiopathology | Rats, Wistar | Minocycline - pharmacology | Blood-Brain Barrier - physiopathology | Male | Infarction, Middle Cerebral Artery - therapy | Metalloproteases - metabolism | Brain Edema - prevention & control | Brain Ischemia - enzymology | Anti-Bacterial Agents - therapeutic use | Matrix Metalloproteinase 9 - metabolism | Neuroprotective Agents - pharmacology | Time Factors | Minocycline - therapeutic use | Hypothermia, Induced - methods | Intracranial Hemorrhages - prevention & control | Disease Models, Animal | Intracranial Hemorrhages - physiopathology | Reperfusion Injury - therapy | Acute Disease | Matrix Metalloproteinase 2 - metabolism | Reperfusion Injury - enzymology | Brain Ischemia - therapy | Rats | Brain Edema - therapy | Brain Ischemia - physiopathology | Blood-Brain Barrier - drug effects | Disease Progression | Blood-Brain Barrier - metabolism | Magnetic Resonance Imaging | Animals | Brain Edema - physiopathology | Intracranial Hemorrhages - therapy | Reperfusion Injury - physiopathology | Anti-Bacterial Agents - pharmacology | Infarction, Middle Cerebral Artery - enzymology | Cerebral ischemia | Reperfusion injury | Index Medicus
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8. Full Text Recognizing action units for facial expression analysis
by Tian, Y.-I and Kanade, T and Cohn, J.F
IEEE Transactions on Pattern Analysis and Machine Intelligence, ISSN 0162-8828, 02/2001, Volume 23, Issue 2, pp. 97 - 115
Most automatic expression analysis systems attempt to recognize a small set of prototypic expressions, such as happiness, anger, surprise, and fear. Such... 
Gold | Image analysis | Eyes | Face recognition | Prototypes | Humans | Mouth | Image sequence analysis | Transient analysis | Facial features | AU combinations | multistate face and facial component models | RECOGNITION | EMOTION | action units | neural network | FACES | COMPUTER SCIENCE, ARTIFICIAL INTELLIGENCE | TEMPLATES | ENGINEERING, ELECTRICAL & ELECTRONIC | MOTION | facial expression analysis | OPTICAL-FLOW | computer vision | facial action coding system | Measurement | Usage | Machine vision | Neural networks | Machine learning | Intelligent devices | Design and construction | Facial expression | Methods | Tracking | Facial | Images | Mathematical models | Furrows | Recognition | Computer vision
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9. Full Text Comparison of microbial and transient expression (tobacco plants and plant‐cell packs) for the production and purification of the anticancer mistletoe lectin viscumin
by Gengenbach, Benjamin B and Keil, Linda L and Opdensteinen, Patrick and Müschen, Catherine R and Melmer, Georg and Lentzen, Hans and Bührmann, Jens and Buyel, Johannes F
Biotechnology and Bioengineering, ISSN 0006-3592, 09/2019, Volume 116, Issue 9, pp. 2236 - 2249
Cancer is the leading cause of death in industrialized countries. Cancer therapy often involves monoclonal antibodies or small‐molecule drugs, but... 
transient protein expression | cancer therapy | molecular farming | plant‐cell pack | recombinant viscumin | design of experiments | A-CHAIN | CRYSTAL-STRUCTURE | TOXIN | GLYCOSYLATION | PREDICTIVE MODELS | plant-cell pack | RECOMBINANT | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ALBUM | PROTEINS | FREE RICIN | HARVEST INDEX | Care and treatment | Biological products | Escherichia coli | Lectins | Monoclonal antibodies | Comparative analysis | Cells | Cancer | Carbohydrates | Plants (botany) | Purification | Polypeptides | Capillary electrophoresis | Chains | Mammals | Mammalian cells | Microorganisms | Tobacco | Inclusion bodies | Electrophoresis | Pharmaceuticals | Index Medicus
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10. Full Text Efficient transient protein expression in tomato cultivars and wild species using agroinfiltration-mediated high expression system
by Hoshikawa, Ken and Fujita, Satoshi and Renhu, Na and Ezura, Kentaro and Yamamoto, Tsuyoshi and Nonaka, Satoko and Ezura, Hiroshi and Miura, Kenji
Plant Cell Reports, ISSN 0721-7714, 1/2019, Volume 38, Issue 1, pp. 75 - 84
The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and wild species with high level of protein... 
Life Sciences | Biotechnology | Transient protein expression | Plant Biochemistry | Agroinfiltration | Tomato | Plant Sciences | Cell Biology | MICRO-TOM | DEPENDENT RNA-POLYMERASE | DNA METHYLATION | REGENERATION | PROTOCOL | PLANT SCIENCES | AGROBACTERIUM | RESISTANCE | OPTIMIZATION | ACCUMULATION | GENETIC-TRANSFORMATION | 4-Aminobutyrate transaminase | Transaminase | Adaptability | Innovations | Gene expression | Transgenic plants | Weight | Fruits | Plant cells | Proteins | Leaves | γ-Aminobutyric acid | Green fluorescent protein | Protein expression | Tomatoes | Species | Cultivars | Index Medicus
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11. Full Text TRPM3 expression in mouse retina
by Brown, R. Lane and Xiong, Wei-Hong and Peters, James H and Tekmen-Clark, Merve and Strycharska-Orczyk, Iwona and Reed, Brian T and Morgans, Catherine W and Duvoisin, Robert M
PLoS ONE, ISSN 1932-6203, 02/2015, Volume 10, Issue 2, pp. e0117615 - e0117615
Transient receptor potential (TRP) channels constitute a large family of cation permeable ion channels that serve crucial functions in sensory systems by... 
BIPOLAR CELLS | CHANNEL | PATHWAY | MULTIDISCIPLINARY SCIENCES | GANGLION-CELLS | LIGHT | PANCREATIC BETA-CELLS | RECEPTOR | NEIBANK PROJECT | IDENTIFICATION | SEQUENCE TAG ANALYSIS | Electroretinography | Retina - drug effects | Cricetinae | Gene Expression | Retina - metabolism | Cricetulus | Mice, Transgenic | Retinal Ganglion Cells - metabolism | Recombinant Fusion Proteins - metabolism | Protein Transport | Animals | Calcium Signaling - drug effects | Protein Isoforms | Pregnenolone - pharmacology | Recombinant Fusion Proteins - genetics | TRPM Cation Channels - genetics | Mice | TRPM Cation Channels - metabolism | Retinal Ganglion Cells - drug effects | CHO Cells | Immunohistochemistry | Sulfates | Neurosciences | Calcium | Science | Fluorescence | Retina | Environmental changes | Pregnenolone | Depolarization | Calcium signalling | Transient receptor potential proteins | Signal transduction | Bipolar cells | Physiology | Ion channels | Calcium (intracellular) | Neurons | Calcium channels (voltage-gated) | Sensory systems | Calcium imaging | Pharmacology | C-Terminus | Retinal ganglion cells | Acids | Pregnenolone sulfate | Mefenamic acid | Neural networks | Information processing | Sulfate | Immunofluorescence | Index Medicus
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12. Full Text Identification of critical cysteine sites in brassinosteroid‐insensitive 1 and novel signaling regulators using a transient expression system
by Huang, Guozhong and Sun, Jianhang and Bai, Jiaojiao and Han, Yufang and Fan, Fenggui and Wang, Shuangfeng and Zhang, Yingying and Zou, Yanmin and Han, Zhifu and Lu, Dongping
New Phytologist, ISSN 0028-646X, 05/2019, Volume 222, Issue 3, pp. 1405 - 1419
The plant hormones brassinosteroids ( BR s) modulate plant growth and development. Cysteine (Cys) residues located in the extracellular domain of a protein are... 
disulfide‐forming cysteine residues | pattern‐triggered immunity compromised receptor‐like cytoplasmic kinase | ) | PCRK | transient system | EMS | Arabidopsis thaliana | BR | ‐ | BES | 1 | insensitive 1 | 1/2 | bri1 | Suppressor 1 | BRI | brassinosteroid | CYTOPLASMIC KINASE | COMPLEX | bri1-EMS-Suppressor 1 (BES1) | BRI1 | PSEUDOMONAS-SYRINGAE | PLANT SCIENCES | BR-insensitive 1 (BRI1) | pattern-triggered immunity compromised receptor-like cytoplasmic kinase (PCRK) 1 | disulfide-forming cysteine residues | GENE-EXPRESSION | brassinosteroid (BR) | RECEPTOR-LIKE PROTEIN | ENDOPLASMIC-RETICULUM | BAK1 | PLANT IMMUNE | INNATE IMMUNITY | Cysteine | Analysis | Cellular signal transduction | Disease susceptibility | Plant hormones | Cystine | Protein binding | Steroids | Residues | Phosphorylation | Regulators | Disulfide bonds | Alanine | Stabilizing | Superhelical DNA | Protoplasts | Innate immunity | BRI1 protein | Brassinosteroids | Identification | Hormones | Leucine | Dephosphorylation | Extracellular | Immunity | Proteins | Signaling | Plant growth | Brassinolide | Protein structure
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