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2012, Methods in molecular biology, ISBN 1617794546, Volume 812., xi, 329
Book
2001, Methods in molecular biology, ISBN 9780896038325, Volume 177, xii, 336
The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect... 
Research | Yeast fungi | Methodology | Plasmids | Protein binding | Cytology | Cell Biology | Life Sciences
Book
2018, Methods in Molecular Biology, ISBN 1493978705, Volume 1794
eBook
1997, Advances in molecular biology, ISBN 9780195109382, xi, 344
Book
Methods in Molecular Biology, ISSN 1064-3745, 2016, Volume 1356, pp. 31 - 41
Many biological processes are regulated by protein-protein interactions, and the analysis of these interactions has been a productive endeavor contributing to... 
Candida albicans | HOG pathway | Protein-protein interaction | Yeast two-hybrid | Saccharomyces cerevisiae - metabolism | Protein Interaction Mapping - methods | Two-Hybrid System Techniques | Saccharomyces cerevisiae Proteins
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 4/2001, Volume 98, Issue 8, pp. 4569 - 4574
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1732, pp. 143 - 157
Mammalian AMP-activated protein kinase (AMPK) is a Ser/Thr protein kinase that acts as a crucial energy sensor in the cell. Since AMPK plays a key role in a... 
AMPK | Bait plasmid | Prey plasmid | Yeast two-hybrid | β-Galactosidase | Saccharomyces cerevisiae - metabolism | Protein Interaction Mapping - methods | Two-Hybrid System Techniques | AMP-Activated Protein Kinases - metabolism | Phosphorylation | Protein Subunits - metabolism | β-galactosidase | prey plasmid | yeast two-hybrid | bait plasmid
Journal Article
Nature methods, ISSN 1548-7091, 12/2014, Volume 11, Issue 12, pp. 1203 - 1206
Journal Article
Journal of Molecular Biology, ISSN 0022-2836, 03/2019, Volume 431, Issue 6, pp. 1160 - 1171
We applied a yeast-two-hybrid (Y2H) analysis to screen for ubiquitin variant (UbV) inhibitors of a human deubiquitinase (DUB), ubiquitin-specific protease 2... 
Mdm2 | yeast two-hybrid | ubiquitin | ubiquitin variants | Ubiquitin Proteasome System | ANTIBODIES | DUBS | STRATEGY | BIOCHEMISTRY & MOLECULAR BIOLOGY | PEPTIDE APTAMERS | USP2A | ENZYMES | RESISTANCE | TARGETS | SELECTION | REVEALS | Ubiquitin | Molecular genetics | Proteases | Analysis | Tryptophan | Fluorescence | Cells
Journal Article
Plant Physiology, ISSN 0032-0889, 9/2012, Volume 160, Issue 1, pp. 477 - 487
Mitogen-activated protein kinase (MAPK) cascades support the flow of extracellular signals to intracellular target molecules and ultimately drive a diverse... 
Proteins | Plant interaction | Yeasts | Cell growth | Social interaction | CDNA libraries | Fluorescence | Antibodies | CELL BIOLOGY AND SIGNAL TRANSDUCTION | Plants | Rice | VISUALIZATION | IMMUNITY | ARABIDOPSIS-THALIANA | PHOSPHORYLATION | MAP KINASE | GENE FAMILY | IDENTIFICATION | EXPRESSION | VECTORS | PROTEOMICS | PLANT SCIENCES | Phosphorylation | Proteome - genetics | Immunoprecipitation | Agrobacterium tumefaciens - genetics | Recombinant Fusion Proteins - metabolism | Protein Interaction Maps | Mitogen-Activated Protein Kinases - analysis | Oryza - genetics | Membrane Proteins - metabolism | Plant Proteins - metabolism | Gene Library | Membrane Proteins - genetics | Tobacco - metabolism | Enzyme Assays - methods | Proteome - analysis | Oryza - enzymology | Onions - metabolism | Plant Proteins - genetics | Protein Interaction Mapping - methods | Two-Hybrid System Techniques | High-Throughput Screening Assays | Agrobacterium tumefaciens - metabolism | Tobacco - genetics | Bacterial Proteins - metabolism | Mitogen-Activated Protein Kinases - genetics | Transcription Factors | Enzyme Activation | Proteome - metabolism | Luminescent Proteins - metabolism | Mitogen-Activated Protein Kinases - metabolism | Plant proteins | Research | Observations | Identification and classification | Interactomes | Mitogen-activated protein kinases
Journal Article
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 07/2018, Issue 7
Generating DNA-binding domain (DB)-bait strains for Gateway-compatible yeast two-hybrid (Y2H) screens involves three steps. The first is to generate an Entry... 
Proteins | Yeast | Screens | Deoxyribonucleic acid--DNA | Strain
Journal Article
FEBS Journal, ISSN 1742-464X, 11/2005, Volume 272, Issue 21, pp. 5391 - 5399
The two‐hybrid method detects the interaction of two proteins by their ability to reconstitute the activity of a split transcription factor, thus allowing the... 
computational methods | protein–protein interaction | Plasmodium falciparum | proteomics | yeast | BIOCHEMISTRY & MOLECULAR BIOLOGY | GENETIC SYSTEM | SACCHAROMYCES-CEREVISIAE | GENOME | CELLULAR NETWORKS | SPLIT-UBIQUITIN | IN-VIVO | PROTEIN-PROTEIN INTERACTIONS | protein-protein interaction | INTERACTION NETWORKS | SCALE DATA
Journal Article